id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-304498-ty41xob0	Denison, Mark R	Coronaviruses: An RNA proofreading machine regulates replication fidelity and diversity	2011-03-01		.txt	text/plain	7332	345	38	Genetic inactivation of exoN activity in engineered SArS-Cov and MHv genomes by alanine substitution at conserved De-D-D active site residues results in viable mutants that demonstrate 15-to 20-fold increases in mutation rates, up to 18 times greater than those tolerated for fidelity mutants of other rNA viruses. Genetic inactivation of exoN activity in engineered SArS-Cov and MHv genomes by alanine substitution at conserved De-D-D active site residues results in viable mutants that demonstrate 15-to 20-fold increases in mutation rates, up to 18 times greater than those tolerated for fidelity mutants of other rNA viruses. The high mutation rates of RNA viruses also render them particularly susceptible to repeated genetic bottleneck events during replication, transmission between hosts or spread within a host, resulting in progressive deviation from the consensus sequence associated with decreased viral fitness and sometimes extinction.	./cache/cord-304498-ty41xob0.txt	./txt/cord-304498-ty41xob0.txt