id author title date pages extension mime words sentences flesch summary cache txt cord-304747-ojyxs3cp Gaynor, Anne M Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections 2007-05-04 .txt text/plain 6665 328 51 Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus–specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The virus was detected in the respiratory secretions from an additional 43 patients in two continents, and the complete genomes of multiple isolates were sequenced. In the early region, an unspliced open reading frame of 194 amino acids was detected that possibly encodes for the STAg. As the paradigm in other polyomaviruses is that STAg is expressed from a spliced message, analysis of potential splice sites revealed the presence of a putative splice donor sequence just one nucleotide 59 of the initially predicted The initial screen used primers targeting the VP2 region, which possessed less than 20% amino acid homology to JC and BK virus to minimize the possibility of cross reactivity with the known human polyomaviruses. ./cache/cord-304747-ojyxs3cp.txt ./txt/cord-304747-ojyxs3cp.txt