id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-306733-df36w6l7	Rosales-Mendoza, Sergio	What Does Plant-Based Vaccine Technology Offer to the Fight against COVID-19?	2020-04-14		.txt	text/plain	8591	420	39	Transient nuclear genome transformation Rapid production; high productivity; implemented at the industrial level Seed bank cannot be generated; requires purification of the antigen to eliminate toxic compounds from the host and ag-robacteria residues S protein; multiepitope vaccines A chimeric protein of GFP and amino acids 1-658 of the SARS-CoV-1 S protein (S1:GFP) was transiently expressed in tobacco leaves and stably transformed in tobacco and lettuce. No immunization assays were performed The SARS-CoV-1 N protein was transiently expressed in Nicotiana benthamiana, which induced in mice high levels of IgG1 and IgG2a and up regulation of IFN-γ and IL-10 in splenocytes. The precedents of SARS-CoV-1 and MERS antigens expressed in recombinant systems leading to the formation of VLPs constitute important guides for the topic of COVID-19 vaccine development. Thus, VLPs based on the main SARS-CoV-2 structural proteins is an attractive approach for vaccine development against coronavirus infections.	./cache/cord-306733-df36w6l7.txt	./txt/cord-306733-df36w6l7.txt