id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309179-5hlatbqe	Bosch, Albert	New tools for the study and direct surveillance of viral pathogens in water	2008-05-26		.txt	text/plain	3352	165	36	Main difficulties to overcome for virus detection and characterisation in water samples encompass viral diversity, occurrence of low particle numbers, particularly in drinking water, and the technical challenges of virus assays. Nucleic acid amplification techniques are currently the most widely used methods for detection of viruses in 298 Environmental Biotechnology water, which also enable to gather information of the virus genotypes occurring in the environment, thus providing most relevant epidemiological information, particularly important for the implementation and follow-up of vaccination programmes [2, 3, 4 ] . Nevertheless, as stated above, no alternative to molecular detection analysis exists for highly health significant waterborne viruses such as human norovirus and hepatitis A virus. Development, evaluation, and standardization of a real-time TaqMan reverse transcription-PCR assay for quantification of hepatitis A virus in clinical and shellfish samples	./cache/cord-309179-5hlatbqe.txt	./txt/cord-309179-5hlatbqe.txt