id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-311410-lgqup9ug	Ayers, M.	A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses	2006-05-02		.txt	text/plain	3106	157	52	In this study we present the design and validation of a single tube RT-PCR assay using a pair of consensus primers for the detection of mosquito-borne flaviviruses. For specificity studies, several viral samples were used, including clinical samples found to contain CMV and EBV DNA by PCR testing, as described (Johnson et al., 2000) ; a clinical isolate of influenza virus from the 2004-2005 season, typed as H3 by sequencing of the hemagglutinin gene; echovirus 11 from the laboratory collection at the Hospital for Sick Children; hepatitis C virus RNA was obtained by in vitro transcription of the infectious clone pCV-H77C (Yanagi et al., 1997) (the clone was kindly provided by Dr. J. Coupled with sequencing, it could detect with great sensitivity and identify several mosquito-borne flaviviruses including WNV, Kunjin, SLE, YFV and dengue fever viruses.	./cache/cord-311410-lgqup9ug.txt	./txt/cord-311410-lgqup9ug.txt