id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-311748-yr2ep7uf	Kahyaoglu, L. N.	11 New approaches in microbial pathogen detection	2013-12-31		.txt	text/plain	8020	381	48	In recent years, polymerase chain reaction (PCR)-based methods in particular, have become the gold standard for virus detection in food due to their high sensitivity, specifi city and potential to detect even a single virus particle (Bosch et al. In recent years, qRT-PCR has been widely used in food virology as the most promising nucleic acid detection method, since it offers several advantages over conventional RT-PCR, including high sensitivity, the possibility of simultaneous amplifi cation, detection and quantifi cation of the target nucleic acids in a single step, and with minimum risk of carry-over contamination through the use of a closed system (Mackay et al. The challenges associated with the detection of foodborne viruses, such as PCR inhibitors and low virus concentrations in foods, affect the effi ciency of realtime assay adversely, therefore, for process control (PC) an internal amplifi cation control (IAC), which is extracted and amplifi ed with the target sequence, is crucial in the evaluation of PCR and to prevent false negatives (Di Pasquale et al.	./cache/cord-311748-yr2ep7uf.txt	./txt/cord-311748-yr2ep7uf.txt