id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-314201-6njwigco	Maher-Sturgess, Sheryl L	Universal primers that amplify RNA from all three flavivirus subgroups	2008-01-24		.txt	text/plain	4625	253	54	Tanaka [3] published the first universal primer pair specific for mosquito borne flaviviruses in 1993; the YF1 and YF3 primers targeted the NS5/3'UTR of the genome and were based upon the six flavivirus sequences available at the time. In 2005 Gaunt and Gould designed a universal nested PCR, using six primers targeting the E gene, capable of amplifying cDNA from 60 flavivirus strains. In the present study, we identified conserved sites and developed a universal, non-nested primer pair that amplifies cDNA from each of the major subgroups of flaviviruses, and also TABV, under standard reaction conditions. Since the amplified products represent 8% of the genome, this is sufficient sequence to determine the species of the virus and thus potentially to identify unrecognised flaviviruses. Rapid subgroup identification of the flaviviruses using degenerate primer E-gene RT-PCR and site specific restriction enzyme analysis	./cache/cord-314201-6njwigco.txt	./txt/cord-314201-6njwigco.txt