id author title date pages extension mime words sentences flesch summary cache txt cord-315037-lmur80te Lin, Chien-Yu Increased Detection of Viruses in Children with Respiratory Tract Infection Using PCR 2020-01-15 .txt text/plain 4190 241 42 We performed a multiplex real-time polymerase chain reaction (PCR) to investigate the viral etiology in pediatric patients and compared the detection rates with those determined using traditional antigen tests and virus cultures. This study aims to detect respiratory viruses in children using PCR and to compare the detection power of this technique against that when using traditional antigen tests and virus cultures. For children with respiratory symptoms and with a clinical suspicion of virus infection, a test for RSV antigen test, human parainfluenza virus (PIV) type 3 antigen test, viral PCR for enterovirus, or viral cultures was prescribed by the judgment of pediatricians. The following multiplex PCR assays were performed for each sample to detect RNA/DNA of 15 respiratory viruses, including RSV A or B, FluA, FluB, human enterovirus (EV), MPV, human parainfluenza virus types 1-4, human rhinovirus (RV), coronavirus OC43/NL63/229E, human adenovirus (ADV), and human bocavirus (Boca). The present study demonstrates that PCR has higher detectability for respiratory viruses compared to traditional antigen tests and viral cultures. ./cache/cord-315037-lmur80te.txt ./txt/cord-315037-lmur80te.txt