id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-315167-ph15z424	Goka, E. A.	Pan-human coronavirus and human bocavirus SYBR Green and TaqMan PCR assays; use in studying influenza A viruses co-infection and risk of hospitalization	2014-12-05		.txt	text/plain	3311	185	52	title: Pan-human coronavirus and human bocavirus SYBR Green and TaqMan PCR assays; use in studying influenza A viruses co-infection and risk of hospitalization This study investigated the association between influenza A viruses co-infection with hBoV and hCoV and severity and the sensitivity of a real-time polymerase chain reaction (RT-PCR) assay for identification of 15 coronaviruses. A series of 217 samples from patients aged 37.7 (SD ± 30.4)] with seasonal influenza A viruses (SeasFluA) identified between 06/2011 and 06/2012 in NW England were tested for hCoV and hBoV using RT-PCR. RESULTS: The limit of detection of hCoV RT-PCR assay was 2 copies/µl of human coronavirus RNA template, a sensitivity comparable to a previously published SYBR green assay for human coronaviruses. This study did not find a significant association between seasonal influenza A viruses co-infection with hCoV and hBoV and severe disease.	./cache/cord-315167-ph15z424.txt	./txt/cord-315167-ph15z424.txt