id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-326225-crtpzad7	Neill, John D.	Simultaneous rapid sequencing of multiple RNA virus genomes	2014-06-01		.txt	text/plain	3804	204	55	This procedure utilized primers composed of 20 bases of known sequence with 8 random bases at the 3′-end that also served as an identifying barcode that allowed the differentiation each viral library following pooling and sequencing. There is a wealth of information in these isolates, but up till now, it has been time consuming and expensive to sequence these viral genomes, often requiring sets of strain-specific primers for PCR amplification and sequencing. These primers were developed so that the 20 base known sequence was used for PCR amplification of the library as well as served as a barcode for identifying each viral library following pooling and sequencing. This virus, a BVDV 1b strain isolated from alpaca (GenBank accession JX297520.1; Table 2 , library 3, barcode 10), was assembled from Ion Torrent data and was found to have only 1 base difference from the sequence determined earlier (data not shown). One virus, library 1, barcode 9, had only 658 viral sequence reads but 94.4% of the genome was assembled.	./cache/cord-326225-crtpzad7.txt	./txt/cord-326225-crtpzad7.txt