id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-326719-p1ma4akz	Enjuanes, Luis	Virus-based vectors for gene expression in mammalian cells: Coronavirus	2003-12-31		.txt	text/plain	5923	282	52	Coronaviruses have several advantages as vectors over other viral expression systems: (1) coronaviruses are single-stranded RNA viruses that replicate within the cytoplasm without a DNA intermediary, making integration of the virus genome into the host cell chromosome unlikely, (2) these viruses have the largest RNA virus genome and, in principle, have room for the insertion of large foreign genes, (3) a pleiotropic secretory immune response is best induced by the stimulation of gut-associated lymphoid tissues, (4) the tropism of coronaviruses may be modified by manipulation of the spike (S) protein allowing engineering of the tropism of the vector, (5) non-pathogenic coronavirus strains infecting most species of interest (human, porcine, bovine, canine, feline, and avian) are available to develop expression systems, and (6) infectious coronavirus cDNA clones are available to design expression systems.	./cache/cord-326719-p1ma4akz.txt	./txt/cord-326719-p1ma4akz.txt