id author title date pages extension mime words sentences flesch summary cache txt cord-334771-uy3s6443 Rao, BL A large outbreak of acute encephalitis with high fatality rate in children in Andhra Pradesh, India, in 2003, associated with Chandipura virus 2004-09-09 .txt text/plain 3672 187 49 Samples obtained were: 54 blood samples, 22 throat swabs, ten CSF samples, and one brain aspirate from 55 patients with encephalitis; five blood samples and nine throat swabs from 13 fever cases; and ten blood samples and one throat swab from ten family contacts (including specimens from the brother and mother of a patient who Methods Cell lines and peripheral blood lymphocyte co-cultures were used to isolate the causative agent from clinical samples. The confirmed Chandipura virus encephalitis group consisted of individuals from whose samples we isolated the virus, viral RNA, or reactive IgM antibodies. The viruses isolated in different cell lines from clinical samples from patients with encephalitis were confirmed as Chandipura virus with various techniques including complement fixation, neutralisation test, and immunofluorescence assay. Moreover, the presence of Chandipura virus RNA in nine patients with encephalitis, all from samples obtained before day 4 after onset of illness, suggests an early viraemic phase of the infection process. ./cache/cord-334771-uy3s6443.txt ./txt/cord-334771-uy3s6443.txt