id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-341303-1iayp8oa	McINTOSH, KENNETH	Immunofluorescence in Diagnostic Virology	2006-12-16		.txt	text/plain	2850	126	47	In the 1930s Parker and Muckenfuss first developed the complement fixation reaction using lymph mixed with antibody and a fresh source of complement.s I n fact, this became the procedure of choice for the laboratory diagnosis of smallpox before and even after the general use of tissue culture for viral isolation. Immunofluorescence (IF) was also recognized early as a potentially valuable tool for detection of viral antigen^,'.^.^ but its use as a procedure that could supplement and, at times, replace tissue culture in diagnostic laboratories has been only gradually gaining acceptance. IF has, of course, been used at several stages in the diagnosis of viral diseases: the measurement of antibody, particularly when there is some highly specific antigen in question, as with Epstein-Barr virus or cytomegalovirus; the identification of viruses in tissue culture; and the direct detection of viruses in body fluids.	./cache/cord-341303-1iayp8oa.txt	./txt/cord-341303-1iayp8oa.txt