id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-342906-51296y8d	Li, Zhiping	Aerosolized avian influenza virus by laboratory manipulations	2012-08-06		.txt	text/plain	4489	230	46	Many of the routine procedures used to process influenza virus for laboratory research, such as centrifugation or mixing, have a high potential of producing aerosols [21] , and the particle load of each has been estimated to be up to 1-5 μm. To this end, this study was designed to monitor the presence of aerosolized H5N1 virus produced by normal procedures used to process the virus for experimental research and by the most frequently associated "accidents" for each, such as container breakage and accidental subcutaneous injection. None of the aerosol samples collected from any group at the time directly prior to processing of the experiment had detectable levels of H5N1, as evidenced by negative reverse transcription (Rt)-PCR and HA text results. Our results provide evidence that many of the laboratory techniques used to process influenza virus for experimental analysis produce aerosols and, thereby, represent significant risks of infection to laboratory personnel and potential spread beyond the laboratory.	./cache/cord-342906-51296y8d.txt	./txt/cord-342906-51296y8d.txt