id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-348161-757c51xw	Petrosova, A.	Development of a highly sensitive, field operable biosensor for serological studies of Ebola virus in central Africa	2007-03-26		.txt	text/plain	5428	264	48	We employed a photo immobilization methodology based on a photoactivatable electrogenerated poly(pyrrole-benzophenone) film deposited upon an indium tin oxide (ITO) modified conductive surface fiber-optic. The photochemically modified optical fibers were tested as an immunosensor for detection of antibodies against Ebola virus, in animal and human sera, by use of a coupled chemiluminescent reaction. In this study we present a newly developed optical immunosensor for detection of antibodies to Ebola virus strains Zaire and Sudan, by using a photoimmobilization methodology based on a photoactivable electrogenerated polymer film. The optical fibers coated with poly(pyrrole-benzophenone) were soaked in diluted solution containing inactivated Ebola virus antigen (approximately 7.5 g/ml, the concentration was determined by Micro BCA Protein assay kit, PIERCE) and irradiated with UV light. The calibration curve obtained from the optical fiber immunosensor and ELISA for the detection of anti-Ebola subtype Zaire antibodies.	./cache/cord-348161-757c51xw.txt	./txt/cord-348161-757c51xw.txt