id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-349249-jwvz1ux2	Singh, Gagandeep	A Minimally Replicative Vaccine Protects Vaccinated Piglets Against Challenge With the Porcine Epidemic Diarrhea Virus	2019-10-22		.txt	text/plain	6553	314	46	To combine the safety and efficacy advantages of inactivated and attenuated PEDV vaccines, respectively, in this study, we tested the hypothesis that subjecting PEDV virions to heat treatment at 44°C for 10 min to reversibly unfold structural proteins, followed by exposure to RNAse to fragment the genome, would result in a vaccine preparation with intact viral structure/antigenicity but highly diminished replicative abilities. to that of the spike protein-specific Abs. Strong virus neutralizing Ab responses, were detected in animals vaccinated with the heat and RNAse treated virions but not in the pigs which received the irradiated viral vaccine. Although the heat and RNAse treated virus culture was amplified after 3 passages in cell culture (Figure 2) , the absence its detection by RT-qPCR (Figure 4) , or immunohistochemistry (Table 1 and Figure 5 ) and the lack of strong Ab responses to the non-structural NP (Figure 3) , in vaccinated pigs prior to challenge indicates that active vaccine viral replication was absent in the host or was undetectable by the techniques used.	./cache/cord-349249-jwvz1ux2.txt	./txt/cord-349249-jwvz1ux2.txt