id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-352054-g7q2z4l5	Kolivoška, Viliam	Electrophoresis on a microfluidic chip for analysis of fluorescence‐labeled human rhinovirus	2007-11-15		.txt	text/plain	3710	190	55	Resolution of the sample constituents (virions, a contaminant present in all virus preparations, and excess dye) was improved upon adaptation of the separation conditions, mainly by adjusting the SDS concentration of the BGE. As the applied instrumentation (a commercial microdevice) utilized a red laser at l ex 630 nm for high-sensitivity detection, virus particles were fluorescence (FL)-labeled prior to analysis and preseparated from the excess of dye by size-exclusion chromatography (SEC). Separation from a contaminant, which also becomes labeled upon reaction of the virus sample with reactive Cy5, and from the excess free dye was improved by addition of SDS and varying its concentration in the BGE. Chip electrophoresis with the finally selected BGE (100 mM borate buffer, pH 8.3, containing 3.1 mM SDS), enabled an extremely rapid assessment of virus purity, and the investigation of bioaffinity reactions of labeled virus with a number of soluble artificial receptor fragments.	./cache/cord-352054-g7q2z4l5.txt	./txt/cord-352054-g7q2z4l5.txt