key: cord-259927-xh9cw9ao
authors: Papadopoulos, Nikolaos G.; Megremis, Spyridon; Kitsioulis, Nikolaos A.; Vangelatou, Olympia; West, Peter; Xepapadaki, Paraskevi
title: Promising approaches for the treatment and prevention of viral respiratory illnesses
date: 2017-07-21
journal: J Allergy Clin Immunol
DOI: 10.1016/j.jaci.2017.07.001
sha: 
doc_id: 259927
cord_uid: xh9cw9ao

Viral respiratory tract infections are the most common human ailments, leading to enormous health and economic burden. Hundreds of viral species and subtypes have been associated with these conditions, with influenza viruses, respiratory syncytial virus, and rhinoviruses being the most frequent and with the highest burden. When considering prevention or treatment of viral respiratory tract infections, potential targets include the causative pathogens themselves but also the immune response, disease transmission, or even just the symptoms. Strategies targeting all these aspects are developing concurrently, and several novel and promising approaches are emerging. In this perspective we overview the entire range of options and highlight some of the most promising approaches, including new antiviral agents, symptomatic or immunomodulatory drugs, the re-emergence of natural remedies, and vaccines and public health policies toward prevention. Wide-scale prevention through immunization appears to be within reach for respiratory syncytial virus and promising for influenza virus, whereas additional effort is needed in regard to rhinovirus, as well as other respiratory tract viruses.

Discuss this article on the JACI Journal Club blog: www.jacionline.blogspot.com.

The respiratory system is one of the main portals of entry for human pathogens. Although precise calculations are challenging because of methodology and inherent variability, the number of potentially infectious viruses we breathe every day can be in the range of many thousands. 1 Thus it is not surprising that viral respiratory tract infections (vRTI) are the most common human diseases, leading to enormous health and economic burden. 2 A wide variety of conditions fall within the spectrum of vRTIs. Many of these are by themselves major public health concerns: influenza, acute bronchiolitis, viral pneumonia, and common colds. Together with their downstream effects (ie, acute exacerbations of asthma and chronic obstructive pulmonary disease [COPD] ), all result in vast amounts of morbidity, mortality, and health care costs, including primary care visits, hospitalizations, and deaths but also inappropriate use of antibiotics, loss of productivity, and effects on quality of life. [3] [4] [5] Respiratory tract viruses have been isolated and characterized during the last century, starting from influenza virus (IFV) in the 1930s and followed by respiratory syncytial virus (RSV), coronaviruses, adenoviruses, and rhinoviruses in the 1950 to 1960s; nevertheless, ''new'' viruses or subtypes, such as human metapneumovirus or rhinovirus C, are still being identified. 6, 7 Even though several of these viruses are typically associated with a clinicopathologic entity (eg, IFV with influenza, RSV with bronchiolitis, and rhinovirus with the common cold), there is also extensive overlap, and it is often difficult to identify the etiologic agent based on clinical grounds alone. 8 Consequently, when considering prevention and treatment of vRTI, potential targets include specific pathogens, the immune response, disease transmission, or just symptoms.

Here we provide an overview of the options and highlight some of the most promising approaches in vRTI treatment, including symptomatic medication, immunomodulatory drugs, antiviral agents, and natural products, as well as in vRTI prevention, ranging from vaccines to immunostimulators and public health policies. This is a vast field, and thus we emphasize advances that might be relevant in tackling the virus-induced aspects of allergic disease, such as asthma exacerbations.

Most mild viral respiratory illnesses are managed symptomatically with over-the-counter medications, such as nasal decongestants, antipyretics/analgesics, antitussives, or expectorants, on which no major improvements are foreseen. Although generally well tolerated for short-term relief, some agents can have adverse effects, especially in young children. Therefore the US Food and Drug Administration has issued a warning against the use of over-the-counter cough and cold products in children younger than 4 years of age. 9 Furthermore, the use of decongestants should be minimized, especially in children, 10 whereas codeine has been restricted in children by the European Medical Agency since 2015. 11 Selective COX inhibitors, such as celecoxib and mesalazine, have been widely used in clinics for their antipyretic, analgesic, and antiinflammatory properties in patients with airway diseases, 12 whereas their combination with neuraminidase inhibitors (NAIs) has significantly improved the survival of IFV-infected mice. 13 Recent studies have revealed a new genus of specialized proresolving lipid mediators (SPMs), including lipoxins, resolvins, protectins, and maresins, enhancing anti-inflammatory, antiviral, and proresolving mechanisms. 14 Medications interfering with prostanoid and lipoxygenase biosynthesis and signaling, thus affecting resolution and SPM switching, such as aspirin and nonsteroidal anti-inflammatory drugs, have been suggested as potential agents modulating antiviral immunity, 15, 16 whereas several SPM resolution agonists are in clinical development programs.

Symptomatic relief can also be sought in severe cases. Noninvasive ventilation can reduce respiratory distress in patients with acute viral bronchiolitis. 17 Very recently, new devices delivering totally conditioned gas (378C at 100% relative humidity) through a very high-flow nasal cannula (up to 60 L/min) have been indicated for bronchiolitis mainly as rescue therapy to reduce the need for admission to the intensive care unit. 18 Immune and antiviral pathway modulators Although vRTIs are most often short-lived events, impaired antiviral clearance and/or activation of inflammatory pathways lead to important downstream complications, such as exacerbations of asthma or COPD. 19 The immune and antiviral mechanisms leading from infection to exacerbation have been scrutinized, 20 and medications targeting these pathways are being evaluated as promising candidates to reduce disease burden. Impaired interferon production has been observed in patients with various obstructive respiratory diseases, potentially contributing to enhanced susceptibility to and/or severity of virus-induced acute airway exacerbations. 21 Although inhaled IFN-b supplementation has not shown a clear effect in preventing virus-induced symptom worsening in patients with mild asthma, subanalysis in patients with severe asthma showed a protective effect. 22 Interestingly, in an experimental model exogenous administration of IFN-l1 induced a strong and more prolonged antiviral state than IFN-b. 23 Moreover, experimental studies in an allergic asthma model showed that IFN-l supplementation enhanced T H 1 immunity by inducing IFN-g and suppressing T H 2 and T H 17 responses through modulation of lung CD11c 1 dendritic cell function. 24, 25 Novel antibody-based drugs with antirhinovirus and immunomodulatory effects act through IFN-b induction and suppression of T H 2 responses in experimental models. 26 The prototype synthetic Toll-like receptor (TLR) 4 antagonist Eritoran (E5564) and anti-TLR4 IgG therapy have been shown to block IFV lethality in mice by suppressing lung pathology, clinical symptoms, and viral titers. 27, 28 Other innate immune receptors, such as TLR2, also have potential for host-targeted therapeutic approaches. 21 Interestingly, omalizumab, an anti-IgE mAb, prevents asthma exacerbations either by decreasing the duration and shedding of rhinovirus infection or by blocking the synergistic effect of rhinovirus infection on allergy. 29, 30 Because high-affinity IgE receptor (FcεRI) cross-linking on plasmacytoid dendritic cells reduces IFN-a responses after viral infections, it is plausible that omalizumab enhances virus-induced IFN-a production in asthmatic patients, thus limiting virus spreading and infection severity. 31 ''Severe cytokine storm,'' an entity associated with markedly higher levels of proinflammatory cytokines, has been associated with severe influenza infections; immunomodulatory agents have been proposed as potential therapeutic strategies. 32 Peroxisome proliferator-activated receptor g agonists (eg, rosiglitazone and pioglitazone) are critical regulators of inflammation and have been promising in improving the clinical outcome of severe influenza infections. 33 Their development slowed down from 2000 to 2005 because of possible cardiovascular side effects; however, in 2015, the US Food and Drug Administration lifted restrictions based on new safety data. 34 Moreover, sphingosine-1-phosphate receptor 1 agonists 1, which are located mainly on pulmonary endothelial cells, exhibit cytokine storm-blunting activity by suppressing both innate cellular and cytokine/chemokine responses, particularly when combined with antiviral agents. 35 There is increasing interest in the use of macrolides to treat or prevent virus-induced asthma exacerbations, although microbial resistance remains a major hurdle, and therefore they are not currently indicated. Early in vivo evidence suggested that azithromycin has anti-inflammatory and antiviral effects through induction of interferon-stimulated gene mRNA expression and reduced viral replication and release in patients with asthma and chronic obstructive lung disease. 36, 37 In a randomized clinical trial including wheezing preschool-aged children, early azithromycin administration significantly reduced the likelihood of a severe lower respiratory tract infection. 38 Novel macrolides (Mycobacterium avium complex 5) with anti-inflammatory, antibacterial, and, more importantly, interferon-augmenting activity in airway epithelium have been identified. 39 Finally, in vitro models have demonstrated that a 1 -antitrypsin exerts anti-inflammatory effects in airway epithelial cells from rhinovirus-infected patients with COPD, potentially through inhibition on caspase-1 activity, suggesting a 1 -antitrypsin as a potential anti-inflammatory agent. 40 Antivirals vRTIs are usually characterized by an acute and self-limiting course, which means that the peak of viral replication usually precedes or parallels the appearance of clinical symptoms. As a result, the time window from verification and/or typing of the pathogen, allowing a specific therapeutic intervention, is extremely narrow. Additional challenges need to be overcome, such as the structural variation of viral proteins, multiple genotypes, and high mutation rates. Accordingly, only a very limited number of specific antiviral drugs are currently licensed, and promising approaches mostly aim to control severe complications, reduce disease burden, or transmission. Antiviral strategies aim to block particular stages of the viral lytic cycle, including attachment and entry to the host cell, replication, transcription, and translation (Fig 1) . 41 In principle, preventing a viral pathogen from entering the host cell represents the ideal antiviral strategy because the virus is not allowed to ''hack'' the host: IFV NAIs have been successfully used to competitively bind the sialic acid-binding pocket of neuroaminidase and are good examples of this approach. Oseltamivir and zanamivir have been used as anti-flu therapies, 42 whereas laninamivir and peramivir show antiviral activity against wild-type but also against oseltamivir-resistant and NAI-resistant strains, respectively. 43, 44 The nonenveloped rhinoviruses use viral capsid structures to bind their receptors (intercellular adhesion molecule 1 [ICAM-1], low-density lipoprotein receptor, and cadherin-related family member 3). 45 Even though more than 50% of rhinovirus strains use ICAM-1 for cell entry, an ICAM-1 competitor, tremacamra, did not make it into the clinic despite initially promising results, 46 and no anti-ICAM-1 drugs are currently available.

Another strategy is to prevent capsid uncoating and further assembly of new virions. This strategy has been successfully used against IFV and severe acute respiratory syndrome (SARS)coronavirus, which use a class I fusion mechanism. 47 DAS181 (Fludase, NexBio, Inc, San Diego, Calif) is a fusion construct that cleaves the sialic acid receptors on host cells, and its antiviral spectrum includes IFV and parainfluenza viruses (PIVs). 48 Nonenveloped viruses, such as rhinovirus, release their genomes through a conformational shift of the capsid proteins accompanied by an expansion of the viral shell along with the opening of symmetry-related channels (pores) from which the genome is released (virus uncoating). 49, 50 Various capsid-binding compounds against rhinoviruses have been tested (R and WIN series) without ultimate success. 51 Pleconaril, BTA798 (vapendavir), and pocapavir (V-073) are still under clinical evaluation. 52 Of note, a major drawback of capsid binders is the rapid emergence of resistance. 52 Several fusion inhibitors are being developed for the treatment of RSV and have been reviewed elsewhere. 3, 53 Because of their limited coding capacity, viruses rely on the production of polyproteins that need to be cleaved into functional subunits by viral proteases. The enterovirus polyprotein is cleaved by a family of cysteine proteases, which are highly conserved among different subtypes but lack homology with human proteases. Unfortunately, after failed attempts with ruprintrivir (AG7088) and AG7404, which showed antiviral activity in vitro but not in vivo, no similar agents are being pursued currently. 52 The use of HIV protease inhibitors, such as lopinavir and ritonavir, in patients with SARS has not been associated with any proved benefit, although retrospective studies reported that severe outcomes (acute respiratory distress syndrome or death) occurred less often in those receiving a combination of lopinavir/ritonavir and ribavirin with corticosteroids. 54 Polymerase inhibitors (nucleoside/nucleotide analogs) act by leading to termination of the polynucleotide chain elongation. Ribavirin has been used for the treatment of severe RSV-related disease in high-risk infants and in combination with protease inhibitors in patients with SARS, but its use has been limited because of cost and unconfirmed efficacy toward severe outcomes. ALS-008176 is a promising orally bioavailable prodrug of the novel RSV replication inhibitor ALS-008112 (a cytidine nucleoside analogue), which inhibits RSV replication. 55 Other promising polymerase inhibitors include amiloride (competitive inhibitor of coxsackie virus B3 RNA polymerase) and GPC-N114 (multiple genera in Picornaviridae) but are still in the early stages. 52 Favipiravir (T-705) is an antiviral drug that selectively inhibits the RNA-dependent RNA polymerase of IFV, as well as several other viruses. 56 Umifenovir has been shown to inhibit various human respiratory RNA viruses, including several strains of IFV-A and IFV-B, RSV, PIV3, and rhinovirus B14. It also demonstrates inhibitory activity against other viruses, enveloped or not, responsible for emerging or globally prevalent infectious diseases. 57 Finally, a most promising but also challenging antiviral approach is through use of antisense oligonucleotides. Antisense oligonucleotides are single-stranded deoxyribonucleotide oligomers with a sequence complementary to a target mRNA transcript. Thus viral genomic RNA or viral mRNA can be targeted directly. Antisense technology and RNA interference have been experimentally explored in targeting measles virus, SARS-coronavirus, coxsackievirus, enteroviruses and rhinoviruses, PIV, human metapneumovirus, IFV, and RSV genomes. 58, 59 The RNA inhibition-based therapeutic that is furthest advanced in clinical development at this time is against RSV. 58 ALN-RSV01 is an unmodified, naked, small interfering RNA designed to inhibit the replication of RSV by interrupting the synthesis of the viral N protein. The sequence of the target is well conserved throughout naturally occurring RSV A and B genotypes. 58 In all, new antivirals are being explored continuously, particularly for life-threatening viruses, such as IFV (influenza) and RSV. Rhinoviruses, even though simple in terms of genome organization and protein coding, have proved extremely difficult to target, mostly because of their high diversity and immuneevading strategies but also to some extent to the underestimation of rhinovirus infection clinical consequences.

Within the past few years, scientific communities all over the world have shown renewed interest in the search for novel immune-stimulating or antiviral agents of plant origin for either treatment or prevention, often using ethnopharmacologic approaches. 60 Natural compounds are widely recognized as privileged structures trimmed by evolutionary processes to interact with macromolecular targets. Plants use a diverse set of biochemical pathways to generate several secondary metabolites representing ecosystem adaptations to help plants to survive various environmental stresses and protect them from infections and infestations. 61 The antiviral potential of plant extracts or compounds varies among viruses. 62 Natural compounds occupy an equally large and complex chemical space as synthetic compounds. In the case of antiviral agents, 80% of 46 entities registered in the last approximately 30 years (1981-2010) can be classified as natural product botanicals, synthetic but natural product mimics, natural product pharmacophores, or a combination of the latter 2. Oseltamivir, a success story in IFV drug synthesis, has its roots in nature: the abundant plant constituents quinic acid and shikimic acid are used as its starting materials. 63 A screening strategy was applied to investigate crude extracts from 260 plant species on their inhibiting potential toward NAIs of Clostridium perfringens. 64 Moreover, 14 bioactive compounds from Cleistocalyx operculatus buds were discovered by using an anti-IFV screening approach. 65 The Chinese Academy of Medical Sciences tested more than 10,000 plants. Among them, a pronounced neuroaminidase-inhibiting effect was observed for the herb extract of Elsholtzia rugulos. Some extracts from Agrimonia pilosa, 66 Echinacea purpurea, 67 and Prunus mume 68 or the multicomponent mixtures polyphenol fractions from Punica granatum 69 and secoiridoid glucosides from Ligustrum lucidum 70 have shown a significant reduction of virus-induced cytopathic effects and in general antiviral or anti-influenza activity.

A 10% to 20% risk reduction of common cold incidence with the use of Echinacea species supplements has been shown. 71 Moreover, a recent meta-analysis demonstrated benefit on long-term (2-4 months) prevention with Echinacea species on recurrent respiratory tract infections (RTIs). 72 Another promising compound is BNO 1016, a fixed combination of 5 herbal substances that significantly reduced symptoms and led to faster recovery in patients with acute viral rhinosinusitis. 73 Reported antiviral effects from natural products, regardless of whether obtained from clinical trials or empiric knowledge, can only give clues for further research. However, it appears that we are entering a new golden age of natural product drug discovery.

Prevention of viral respiratory illness is attempted by either avoiding exposure or strengthening immune defenses, either nonspecifically with immunostimulators or specifically with vaccines. Often, but not always, interventions are targeted toward high-risk groups for a particular infection (eg, RSV in infants and the elderly and IFV in patients with asthma).

A variety of compounds (of microbial, herbal, or synthetic origin) have been used and are still being developed as nonspecific immunostimulatory agents to enhance or modulate the immune response against respiratory pathogens in a preventive or sometimes also therapeutic context. The effectiveness of these agents is usually moderate, and therefore they are only used as secondary supportive measures. As such, however, their potential should not be underestimated.

Among several agents based on bacterial components (OM-85 BV, LW 50020, PMBL, D53, and RU 41740), OM-85 BV, a lyophilisate of water-soluble fractions of bacteria commonly detected in patients with RTIs, has been extensively studied, and a role in the prevention of both acute and recurrent RTIs has been shown. 74, 75 Mechanistic studies have confirmed pleiotropic immunomodulating effects on both innate and adaptive immunity. 76, 77 Pidotimod, a synthetic dipeptide molecule, induces a variety of immunomodulatory effects 78, 79 and has shown some efficacy in preventing RTIs, although this was not always confirmed. 80, 81 Probiotic supplementation has been shown to reduce the incidence, duration, and severity of upper respiratory tract infections through immune modulation 82 and in particular rhinovirus infection through altering nasal innate inflammatory responses. 83 Vitamin D (25-hydroxyvitamin D) has a modulatory role in host defense, inflammation, immunity, and epithelial repair after respiratory tract infections. 84 A recent meta-analysis has confirmed that vitamin D supplementation reduces the overall risk of acute respiratory tract infections. 85 Data from in vitro rhinovirus-infected human primary bronchial epithelial cells showed that exogenous vitamin D can reduce rhinovirus replication through increasing interferon and cathelicidin gene expression. 86 A significant amount of research is still dedicated to the efficacy of vitamin D supplementation, although not without controversy. Hopefully, specific indications will be consolidated soon.

Despite widespread use and a multitude of studies, the role of vitamins C or zinc supplements is still inconclusive in relation to their action against the common cold. 87 Interestingly, meditation and exercise might significantly contribute to the reduction of RTI burden, 88 suggesting that the immunostimulatory capacity of nonpharmacologic measures should also be considered.

The high transmission rate and epidemic nature of respiratory tract viruses indicate that effective public health measures to reduce transmission can have a substantial role in the overall prevention of these infections. A plethora of studies and metaanalyses delineated the important contribution of health policies in reducing transmission of epidemic respiratory tract viruses. In an elegant randomized control trial, an automated Web-based intervention that maximized handwashing intention was associated with fewer episodes of influenza-like illness, shorter duration of symptoms, and fewer antibiotic prescriptions in the intervention group. 89 Although similar results regarding handwashing have been confirmed in a Cochrane meta-analysis, 90 hand hygiene interventions in educational settings were not as unequivocally effective. 91, 92 Low adherence to hand hygiene recommendations was correlated with higher incidence of IFV infection among health care workers during the 2009 pandemic. 93 The use of face masks has been shown to be highly effective in the interruption of respiratory viral spread. 90 This has been demonstrated further in a cluster randomized trial in which a reduced odds ratio of influenza infection secondary attack was observed in the intervention group. 94 Face masks are now regularly worn in some communities, especially in Asia, but much less so in western societies. Taken together, it seems that public health measures might provide a valuable ally in decreasing the burden of respiratory tract infections in the community.

Both vaccines and mAbs (passive immunization) are relevant interventions. Vaccines for IFV, rhinovirus, and RSV were initially developed as long ago as the 1940s to 1960s, although with mixed success, mostly because of rapid virus evolution. Improved understanding of vaccine immunology and technologic developments place us now closer than ever to developing highly effective vaccines against the major respiratory tract viruses. mAb therapies to viral infections, such as EBV (rituximab) or RSV (palivizumab), provide passive immunization and are licensed, whereas similar agents targeting influenza and other viruses are in preclinical development. 95 Neutralizing antibodies can bind and inactivate viruses, inhibit viral cell entry (blocking receptor binding or conformational changes), prevent the release of virions from the cell, or modulate immune effector functions. 96, 97 Engineering and production strategies to produce antibody fragments, higher-affinity binding, and longer half-life are contributing to a lower overall cost for therapy, 95 although vaccines are still considered preferable in most cases. It is notable that effective neutralizing mAb epitopes can also inform the rational design of vaccines. 98 Different types of vaccines to respiratory viruses exist, and these are shown in Fig 2. Traditionally, either live attenuated or inactivated viruses are used. More recently, subunit vaccines made of detergent-disrupted whole viruses or purified viral proteins are also common. Furthermore, promising approaches use microparticle/nanoparticle material and recombinant technologies to produce broadly immunogenic, often self-adjuvanting, reproducible, and safe vaccine responses. 99 These delivery systems include synthetic polymers, virosomes, virus-like particles (VLPs), liposomes, lipid nanoparticles, proteins, emulsions, and immune-stimulating complexes. 22 Currently, naturally occurring particles are favored because of safety concerns, 100 even though synthetic polymers, such as poly lactic-co-glycolic acid, are in use, 101 and gold nanoparticles have shown promising results. 102 Self-assembling protein nanoparticles, such as ferritin cages and vaults, have also shown promising preclinical data. 103, 104 Layer-by-layer peptide-fabricated vaccine containing alternately charged poly-L-glutamic acid and poly-L-lysine layers with RSV peptides added have been efficacious in animals. 105 A virosomal adjuvanted vaccine composed of reconstituted IFV envelope, effectively removing the core proteins and RNA, has been available for years with excellent tolerability and efficacy. 106 Several VLP vaccines based on hepatitis B virus surface antigen have been approved for viral infections, such as human papilloma virus 107 and other microbes (eg, malaria 108 ), although an IFV candidate has not progressed. 109 Nevertheless these and other VLPs offer promise because of their valency, similar immune presentation to pathogens, and antigenic preservation. 110 Adjuvants form a vital part of many vaccines; however, only aluminum hydroxide and oil in water emulsions are currently approved. A number of novel adjuvants, such as microcrystalline tyrosine, Matrix M, pathogen-associated molecular patterns, and chitosan, are in development. [111] [112] [113] [114] DNA and RNA vaccines induce an immune response to the nucleic acid-encoded antigen. 115 Impressive results have been reported in animals for a single low-dose intradermal, nonreplicating DNA vaccine for RSV; however, whether this will translate effectively to human subjects is not yet known. 116 To enhance immunogenicity, RNA vaccines have been encapsulated in nanoparticles, achieving sterilizing immunity for Zika virus in mice, 117 as well as being incorporated into virus-based self-replicating constructs known as replicons. 118, 119 Active IFV vaccination already forms the core of the global strategy against severe seasonal and pandemic influenza. Trivalent and more recent quadrivalent vaccines are largely efficacious in healthy adults provided an adequate match between circulating and vaccine strains. 120 Higher-dose (60 mg) and MF59-adjuvanted vaccines are available for elderly patients. 121, 122 Similarly, pandemic vaccines can offer greater cross-clade protection because of the presence of improved (AS03 or MF59) adjuvants. 123 The current frontier of IFV vaccine development is ''universal'' vaccines (Table I) . Ideally, these would protect not only from circulating and pandemic strains but also from novel epitopes that might evolve in the future. Many such vaccines are currently in preclinical and early clinical stages.

Heterosubtypic cross-reactive antibodies to IFV-A against the hemagglutinin (HA) stalk 147 have been isolated from immune subjects, 148 leading to mAbs now in phase 2. 98,124 Similar multilineage HA-stalk antibodies to IFV-B have also been reported. 149 Other conserved proteins have also been targeted, and an anti-M2e antibody is in development. 150 Therefore passive immunization or postinfection treatment might soon become another tool to combat IFV. 125, 126, 151 HA-stalk and chimeric head/stalk-based vaccines have also shown encouraging preclinical results. 103, [152] [153] [154] A further vaccine strategy based on conserved epitopes in proteins, such as M1, NP, and PB1, involves induction of CD4 1 and CD8 1 T-cell immunity, 155 leading to development of a promising MVA viral vector vaccine. Other vaccines use multiepitope peptides to induce IFV-specific T-cell responses, reducing viral shedding in human subjects. 127, 133 Self-replicating RNA nanoparticles also encoding multiple proteins and hepatitis B virus-based VLPs expressing M2e and HA epitopes also appear promising. 156 There are currently no licensed vaccines and only 1 mAb (palivizumab) approved for the prevention of RSV infection. However, there are numerous candidates in clinical trials, as recently reviewed. 3 Suptavumab, an anti-F mAb, 157 has reached phase III trials in preterm infants. MEDI8897 offers 9-fold greater potency than palivizumab and has extended half-life in primates, suggesting a once per season dosing. 128 Candidate vaccines are based on live attenuated strains, subunit, vector, and nanoparticle technologies with a range of adjuvants. Chimeric and combination vaccines using expression vectors in VLPs show much promise. 158 Recent preclinical results exhibit effective neutralization of RSV. [159] [160] [161] [162] [163] The most advanced of these is the Novavax F-protein VLP nanoparticle vaccine with aluminum hydroxide adjuvant, which is in phase III for maternal vaccination. 129, 130 Transplacental transmission of neutralizing antibodies has been demonstrated in preclinical studies, although this has not conferred significant protection from RSV. 164 Recombinant DNA vaccines are also promising because of their apparent ability to induce a balanced T H 1/T H 2 response, with a broad IgG/IgA profile mimicking live RSV challenge. 165 Intranasal and oral vaccine formulations are now in the early stages of clinical studies. 166 FIG 2. Vaccine types. A, Live attenuated vaccines are grown in culture to make them less virulent but can have the problem of reversion. B, Inactivated vaccines are treated with UV or formaldehyde to crosslink proteins and make them nonviable. C, Proteins can be purified, extracted, or dissolved by using detergents. D, Naked nucleic acids are also used as vaccines. E, Nanoparticle vaccines encompass natural and synthetic materials. Membranes can be used to make liposomes to contain and deliver an antigen to a target cell. F, Viruses can have nucleic acid and core protein removed to form virosomes. G, Viral proteins, such as HA stalks or antigens, can be engineered onto immunogenic core proteins (eg, ferritin or vaults). This example is HA on ferritin adapted from PBD codes 3BVE and 5C0S. H, Viruses, such as the vaccinia virus Ankara, with coat proteins and genetic material removed can be engineered to express other antigens, such as influenza M2 ion channel protein. I, VLPs can be engineered to express antigens and naturally glycosylated proteins and have adjuvants incorporated into the coat. PAMP, Pathogen-associated molecular pattern. J, Synthetic nanoparticles made from polymers (polystyrene or poly lactic-co-glycolic acid), gold, or carbon nanotubes can have peptides adsorbed, admixed, or encapsulated. Ag, Antigen. Initial vaccination attempts 167 and more recent preclinical experiments show that inactivated rhinovirus vaccines are type specific and not cross-neutralizing. 8 However, although in animals 168 rhinovirus antibody responses might be weakly cross-neutralizing, data from human subjects suggest that responses are mainly misdirected to internal epitopes. 169 Understanding the full extent of rhinovirus diversity would probably be required to develop a panspecies vaccine.

Multiple strategies are being developed to reduce the burden of viral respiratory illnesses. It is likely that many of these strategies will find a relevant indication: antiviral strategies will most probably make sense in severe life-threatening situations or when a window of opportunity is clearly present, such as in specific virus seasons and susceptible populations. Ideally, prevention at a wide scale through immunization will be able to reduce the overall burden of respiratory infections with a huge effect. This appears to be within reach for RSV and IFV, whereas additional effort is needed toward rhinovirus. In the meantime, symptomatic and immunostimulatory measures provide relief, and they hold promise in relation to postviral reactive airway disease. Public health measures should be expanded because they can be critical in reducing the effect and contain potential epidemics. 

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Global epidemiology of non-influenza RNA respiratory viruses: data gaps and a growing need for surveillance

Lower respiratory tract infection caused by respiratory syncytial virus: current management and new therapeutics

Estimates of hospitalization attributable to influenza and RSV in the US during 1997-2009, by age and risk status

Asthma research in Europe: a transformative agenda for innovation and competitiveness

Influenza: exposing the true killer

Identification of new respiratory viruses in the new millennium

A polyvalent inactivated rhinovirus vaccine is broadly immunogenic in rhesus macaques

Over-the-counter medications: update on cough and cold preparations

limits and danger of ephedrine and pseudoephedrine as nasal decongestants

Comprehensive evidence-based review on European antitussives

Cyclooxygenase 2: its regulation, role and impact in airway inflammation

Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus

The resolution code of acute inflammation: novel pro-resolving lipid mediators in resolution

Targeted prostaglandin E2 inhibition enhances antiviral immunity through induction of type I interferon and apoptosis in macrophages

Role of the lipoxygenase pathway in RSV-induced alternatively activated macrophages leading to resolution of lung pathology

Non-invasive ventilation improves respiratory distress in children with acute viral bronchiolitis: a systematic review

High-flow warm humidified oxygen versus standard low-flow nasal cannula oxygen for moderate bronchiolitis (HFWHO RCT): an open, phase 4, randomised controlled trial

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Viruses and bacteria in acute asthma exacerbations-a GA(2) LEN-DARE systematic review

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The effect of inhaled IFN-beta on worsening of asthma symptoms caused by viral infections. A randomized trial

Efficacy of IFN-lambda1 to protect human airway epithelial cells against human rhinovirus 1B infection

IL-28A (IFN-lambda2) modulates lung DC function to promote Th1 immune skewing and suppress allergic airway disease

Host DNA released by NETosis promotes rhinovirus-induced type-2 allergic asthma exacerbation

Efficacy of novel antibody-based drugs against rhinovirus infection: in vitro and in vivo results

The TLR4 antagonist Eritoran protects mice from lethal influenza infection

The toll-like receptor 4 antagonist Eritoran protects mice from lethal filovirus challenge

Preseasonal treatment with either omalizumab or an inhaled corticosteroid boost to prevent fall asthma exacerbations

Omalizumab is associated with reduced acute severity of rhinovirus-triggered asthma exacerbation

Interferon at the crossroads of allergy and viral infections

The cytokine storm of severe influenza and development of immunomodulatory therapy

Immunomodulatory therapy for severe influenza

FDA Drug Safety Communication: FDA eliminates the Risk Evaluation and Mitigation Strategy (REMS) for rosiglitazone-containing diabetes medicines

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Azithromycin induces anti-viral responses in bronchial epithelial cells

Azithromycin induces anti-viral effects in cultured bronchial epithelial cells from COPD patients

Early administration of azithromycin and prevention of severe lower respiratory tract illnesses in preschool children with a history of such illnesses: a randomized clinical trial

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The anti-inflammatory effect of alpha-1 antitrypsin in rhinovirus-infected human airway epithelial cells

Current progress in antiviral strategies

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Drugs to cure avian influenza infection-multiple ways to prevent cell death

Viral uncoating is directional: exit of the genomic RNA in a common cold virus starts with the poly-(A) tail at the 3'-end

Picornavirus RNA is protected from cleavage by ribonuclease during virion uncoating and transfer across cellular and model membranes

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ALS-008176 for respiratory syncytial virus infection

Favipiravir (T-705), a novel viral RNA polymerase inhibitor

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Gene knockdown in human rhinovirus 1b using 2'-OMe-modified siRNAs results in the reactivation of the interferon response

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Longevity extension by phytochemicals

A review on antiviral activity of the Himalayan medicinal plants traditionally used to treat bronchitis and related symptoms

Influenza neuraminidase: a druggable target for natural products

Neuraminidase inhibitors from Reynoutria elliptica

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C-methylated flavonoids from Cleistocalyx operculatus and their inhibitory effects on novel influenza A (H1N1) neuraminidase

Anti-viral properties and mode of action of standardized Echinacea purpurea extract against highly pathogenic avian influenza virus (H5N1, H7N7) and swine-origin H1N1 (S-OIV)

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Pomegranate (Punica granatum) purified polyphenol extract inhibits influenza virus and has a synergistic effect with oseltamivir

In vitro evaluation of secoiridoid glucosides from the fruits of Ligustrum lucidum as antiviral agents

Echinacea for preventing and treating the common cold

Echinacea reduces the risk of recurrent respiratory tract infections and complications: a meta-analysis of randomized controlled trials

Herbal drug BNO 1016 is safe and effective in the treatment of acute viral rhinosinusitis

Impact of a mixed bacterial lysate (OM-85 BV) on the immunogenicity, safety and tolerability of inactivated influenza vaccine in children with recurrent respiratory tract infection

Clinical and immunological benefits of OM-85 bacterial lysate in patients with allergic rhinitis, asthma, and COPD and recurrent respiratory infections

OM-85 is an immunomodulator of interferon-beta production and inflammasome activity

Bacterial lysate increases the percentage of natural killer T cells in peripheral blood and alleviates asthma in children

Pidotimod: the state of art

Metabolomic profile of children with recurrent respiratory infections

Pidotimod for the prevention of acute respiratory infections in healthy children entering into daycare: A double blind randomized placebo-controlled study

Efficacy and safety of pidotimod in the prevention of recurrent respiratory infections in children: a multicentre study

Probiotics for preventing acute upper respiratory tract infections

Effect of probiotic on innate inflammatory response and viral shedding in experimental rhinovirus infection-a randomised controlled trial

Vitamin D modulation of innate immune responses to respiratory viral infections

Vitamin D supplementation to prevent acute respiratory tract infections: systematic review and meta-analysis of individual participant data

Vitamin D increases the antiviral activity of bronchial epithelial cells in vitro

Prevention and treatment of the common cold: making sense of the evidence

Meditation or exercise for preventing acute respiratory infection: a randomized controlled trial

An internet-delivered handwashing intervention to modify influenza-like illness and respiratory infection transmission (PRIMIT): a primary care randomised trial

Physical interventions to interrupt or reduce the spread of respiratory viruses

Effectiveness of hand hygiene interventions in reducing illness absence among children in educational settings: a systematic review and meta-analysis

Can a school-based hand hygiene program reduce asthma exacerbations among elementary school children?

Risk factors for influenza among health care workers during 2009 pandemic

The role of facemasks and hand hygiene in the prevention of influenza transmission in households: results from a cluster randomised trial

Monoclonal antibodies for prophylactic and therapeutic use against viral infections

The growth and potential of human antiviral monoclonal antibody therapeutics

Converting monoclonal antibody-based immunotherapies from passive to active: bringing immune complexes into play

Antibody recognition of a highly conserved influenza virus epitope

Harnessing nanoparticles for immunomodulation and vaccines

Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles

Current advances in research and clinical applications of PLGA-based nanotechnology

Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2 and H5N1 influenza A viruses

Hemagglutinin-stem nanoparticles generate heterosubtypic influenza protection

Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies

Layer-by-layer nanoparticle vaccines carrying the G protein CX3C motif protect against RSV infection and disease

Eleven years of Inflexal V-a virosomal adjuvanted influenza vaccine

Human papillomavirus and HPV vaccines: a review

AS01 malaria vaccine with or without a booster dose in infants and children in Africa: final results of a phase 3, individually randomised, controlled trial

M2e-based universal influenza A vaccine

Virus-like particles as a highly efficient vaccine platform: diversity of targets and production systems and advances in clinical development

Comparison of a novel microcrystalline tyrosine adjuvant with aluminium hydroxide for enhancing vaccination against seasonal influenza

Matrix M H5N1 vaccine induces cross-H5 clade humoral immune responses in a randomized clinical trial and provides protection from highly pathogenic influenza challenge in ferrets

Safety and immunogenicity of a recombinant M2e-flagellin influenza vaccine (STF2.4xM2e) in healthy adults

Chitosan-a versatile semi-synthetic polymer in biomedical applications

Genetic immunization is a simple method for eliciting an immune response

Development of an intradermal DNA vaccine delivery strategy to achieve single-dose immunity against respiratory syncytial virus

Modified mRNA vaccines protect against Zika virus infection

DNA and RNA-based vaccines: principles, progress and prospects

Replicon RNA viral vectors as vaccines

Transforming growth factor-beta promotes rhinovirus replication in bronchial epithelial cells by suppressing the innate immune response

Efficacy and safety of high-dose influenza vaccine in elderly adults: a systematic review and meta-analysis

Effectiveness of MF59-adjuvanted seasonal influenza vaccine in the elderly: a systematic review and meta-analysis

A systematic review and meta-analysis of cross-reactivity of antibodies induced by oil-in-water emulsion adjuvanted influenza H5N1 virus monovalent vaccines

Preclinical pharmacokinetics of MHAA4549A, a human monoclonal antibody to influenza A virus, and the prediction of its efficacious clinical dose for the treatment of patients hospitalized with influenza A

Efficacy and safety of treatment with an anti-m2e monoclonal antibody in experimental human influenza

Safety and upper respiratory pharmacokinetics of the hemagglutinin stalk-binding antibody VIS410 support treatment and prophylaxis based on population modeling of seasonal influenza A outbreaks

Priming by a novel universal influenza vaccine (Multimeric-001)-a gateway for improving immune response in the elderly population

A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants

Safety and immunogenicity of a Sf9 insect cell-derived respiratory syncytial virus fusion protein nanoparticle vaccine

Immunogenicity and safety of a respiratory syncytial virus fusion protein (RSV F) nanoparticle vaccine in older adults

Evaluation of the immunogenicity and safety of different doses and formulations of a broad spectrum influenza vaccine (FLU-v) developed by SEEK: study protocol for a single-center, randomized, double-blind and placebo-controlled clinical phase IIb trial

Caparr os-Wanderley W. Synthetic Influenza vaccine (FLU-v) stimulates cell mediated immunity in a double-blind, randomised, placebo-controlled phase I trial

A synthetic influenza virus vaccine induces a cellular immune response that correlates with reduction in symptomatology and virus shedding in a randomized phase Ib live-virus challenge in humans

A T cell-inducing influenza vaccine for the elderly: safety and immunogenicity of MVA-NP1M1 in adults aged over 50 years

A Phase IIA study to assess the safety and efficacy of a new influenza candidate vaccine MVA-NP1M1 in healthy adults-Flu002 Clinical Study Report

Evaluating the immunogenicity and safety of a BiondVax-developed universal influenza vaccine (Multimeric-001) either as a standalone vaccine or as a primer to H5N1 influenza vaccine

Back to the future: immunization with M-001 prior to trivalent influenza vaccine in 2011/12 enhanced protective immune responses against 2014/15 epidemic strain

Safety and immunogenicity of multimeric-001-a novel universal influenza vaccine

Implication of respiratory syncytial virus (RSV) F transgene sequence heterogeneity observed in Phase 1 evaluation of MEDI-534, a live attenuated parainfluenza type 3 vectored RSV vaccine

Phase-I study MEDI-534, of a live, attenuated intranasal vaccine against respiratory syncytial virus and parainfluenza-3 virus in seropositive children

A randomized, blinded, controlled, dose-ranging study of a respiratory syncytial virus recombinant fusion (F) nanoparticle vaccine in healthy women of childbearing age

Efficacy of motavizumab for the prevention of respiratory syncytial virus disease in healthy Native American infants: a phase 3 randomised double-blind placebo-controlled trial

Motavizumab for prophylaxis of respiratory syncytial virus in high-risk children: a noninferiority trial

Trivalency of a nanobody specific for the human respiratory syncytial virus fusion glycoprotein drastically enhances virus neutralization and impacts escape mutant selection

Efficacy, safety, and pharmacokinetics of a new 10% liquid intravenous immunoglobulin containing high titer neutralizing antibody to RSV and other respiratory viruses in subjects with primary immunodeficiency disease

Treatment with novel RSV Ig RI-002 controls viral replication and reduces pulmonary damage in immunocompromised Sigmodon hispidus

Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses

Broadly cross-reactive antibodies dominate the human B cell response against 2009 pandemic H1N1 influenza virus infection

Highly conserved protective epitopes on influenza B viruses

Human antibodies reveal a protective epitope that is highly conserved among human and nonhuman influenza A viruses

Tackling influenza with broadly neutralizing antibodies

Influenza virus vaccine based on the conserved hemagglutinin stalk domain

Chimeric hemagglutinin influenza virus vaccine constructs elicit broadly protective stalk-specific antibodies

Chimeric hemagglutinin constructs induce broad protection against influenza B virus challenge in the mouse model

Epitope specificity plays a critical role in regulating antibody-dependent cell-mediated cytotoxicity against influenza A virus

Influenza vaccine research funded by the European Commission FP7-Health-2013-Innovation-1 project

Antibodies to watch in 2017

Protective efficacy and immunogenicity of an adenoviral vector vaccine encoding the codon-optimized F protein of respiratory syncytial virus

Immunogenicity of RSV F DNA vaccine in BALB/c Mice

RSV fusion (F) protein DNA vaccine provides partial protection against viral infection

Chimeric virus-like particles containing a conserved region of the G protein in combination with a single peptide of the M2 protein confer protection against respiratory syncytial virus infection

Baculovirus-expressed virus-like particle vaccine in combination with DNA encoding the fusion protein confers protection against respiratory syncytial virus

Co-immunization with virus-like particle and DNA vaccines induces protection against respiratory syncytial virus infection and bronchiolitis

Respiratory syncytial virus: infection, detection, and new options for prevention and treatment

Immunogenicity and efficacy of codon optimized DNA vaccines encoding the F-protein of respiratory syncytial virus

Mucosal vaccines against respiratory syncytial virus

Prevention of colds by vaccination against a rhinovirus: a report by the scientific committee on common cold vaccines

Challenges in developing a cross-serotype rhinovirus vaccine

Misdirected antibody responses against an N-terminal epitope on human rhinovirus VP1 as explanation for recurrent RV infections