key: cord-284498-54j6ys8s
authors: Ihsanullah, Ihsanullah; Bilal, Muhammad; Naushad, Mu.
title: Coronavirus 2 (SARS-CoV-2) in water environments: Current status, challenges and research opportunities
date: 2020-10-16
journal: nan
DOI: 10.1016/j.jwpe.2020.101735
sha: 
doc_id: 284498
cord_uid: 54j6ys8s

The outbreak of COVID-19 has posed enormous health, social, environmental and economic challenges to the entire human population. Nevertheless, it provides an opportunity for extensive research in various fields to evaluate the fate of the crisis and combat it. The apparent need for imperative research in the biological and medical field is the focus of researchers and scientists worldwide. However, there are some new challenges and research opportunities in the field of water and wastewater treatment concerning the novel coronavirus 2 (SARS-CoV-2). This article briefly summarizes the latest literature reporting the presence of SARS-CoV-2 in water and wastewater/sewage. Furthermore, it highlights the challenges, potential opportunities and research directions in the water and wastewater treatment field. Some of the significant challenges and research opportunities are the development of standard techniques for the detection and quantification of SARS-CoV-2 in the water phase, assessment of favorable environments for its survival and decay in water; and development of effective strategies for elimination of the novel virus from water. Advancement in research in this domain will help to protect the environment, human health, and managing this type of pandemic in the future.

The current global pandemic of COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been growing briskly [1, 2] . Although the major transmission routes of SARS-CoV-2 are through respiratory droplets and direct contact [3] [4] [5] , recent studies have reported the presence of viral RNA of SARS-CoV-2 in untreated and treated wastewater and human feces [3, [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] . In most of the studies, the samples were collected from wastewater treatment plants (WWTPs), while one study reported in the presence of SARS-CoV-2 in wastewater from a cruise ship and commercial passenger aircraft [22] . All the reported samples were collected during the period from Janruary 2020 to July 2020. Recent studies conducted in J o u r n a l P r e -p r o o f the Netherlands and France confirmed that a reasonably high viral load of SARS-CoV-2 RNA is found in the sewage/wastewater [19, 23] .

The presence of SARS-CoV-2 in feces and municipal wastewater poses a severe threat to the environment due to its potential spread via these routes [24] [25] [26] [27] [28] . Therefore, there are serious concerns regarding the spread of SARS-CoV-2 through virus-laden aerosols-borne and fecal-oral routes [8, [29] [30] [31] [32] [33] [34] [35] [36] [37] [38] . It is important to take the necessary precautions to limit the spread of the virus in the environment [39] .

Nevertheless, it creates some new environmental challenges that demand an imperative need for research. Development of effective standard techniques for the detection and quantification of SARS-CoV-2 in water, assessment of the existing water purification technologies and development of novel advanced water treatment systems are major challenges and open research opportunities. Furthermore, careful surveillance of water and wastewater to be used as an early warning tool for such outbreaks in future, understanding the survival and decay mechanism of the novel virus in water and wastewater, analysis of potential pathways of SARS-CoV-2 into water bodies are other potential research opportunities for environmental researchers [40] [41] [42] [43] [44] . The major challenges and opportunities in the field of water and wastewater research are presented in Fig. 1 .

The objective of this article is to highlight the potential opportunities and challenges in the field of water research concerning the novel SARS-CoV-2 virus. The directions for future research to safeguard human health, environment, predict and manage this type of pandemic in the future, are also provided.

J o u r n a l P r e -p r o o f Numerous studies have reported the presence of pathogenic viruses that enter into the water bodies through different sources [45, 46] . Previous studies have demonstrated that SARS-CoV-2 can enter into the water bodies from hospital wastewater and sewage [5, [47] [48] [49] . The waste and wastewater discharged from the quarantine facilities, airports/seaports, residential buildings of infected humans and animals, are the potential sources of SARS-CoV-2 than can enter into the water bodies. The SARS-CoV-2 may also find its ways to groundwater through possible leaching and infiltrations of effluents from health care facilities, sewage, solid landfill and drainage water.

Another important source of viral contamination to the water environment is the leakage from sewage pipes. The possible sources and pathways of SARS-CoV-2 in water systems are depicted in Fig. 2 .

Due to the fast-growing number of patients worldwide, the virus concentration in wastewater/ sewage is expected to increase rapidly. The potential pathways of SARS-CoV-2 into wastewater are via shedding in the stool (for the viable virus), while through urine and stool (for the nonviable virus) [50] . A careful and extensive investigation of all the potential sources of water contamination by SARS-CoV-2 is needed that potentially includes wastewater dispatches from hospitals and greywater from washing personal protective equipment (PPE), surfaces and floors [51] . Table 1 summarizes the literature reporting the presence of SARS-CoV-2 RNA in wastewater, and untreated water in various parts of the world [3, 17, 19, 42] . The concentrations of SARS-CoV-2 RNA in untreated wastewater was in the range of 5.6 × 10 to 4.6 × 10 8 copies per liter [6, 7, 14, 16, 52, 53] . Some studies also reported the detection of SARS-CoV-2 in treated water samples [3, 16] . Previous reports suggested that SARS-CoV-2 survival in an aqueous environment is strongly dependent on the characteristics of water/wastewater [54] [55] [56] . The pH, temperature, presence of antagonistic bacteria, organic matter, sunlight and oxidants might affect the survival of SARS-CoV-2 in aqueous environmmnet [54, 57, 58] . A previous study reported that the inactivation of coronaviruses in the water was highly dependent on the level of organic matter, temperature, and presence of antagonistic bacteria [54] . The suspended solids and organic matter present in water can provide protection for viruses that adsorb to these particles, and they can survive up to several days [54] . However, some published reports suggested that coronaviruses are very sensitive to high temperature and oxidants, such as chlorine [57] .

Studies have reported that surrogate coronaviruses remained infectious in water and sewage for days to weeks [46] . A recent study estimated that the half-life of SARS-CoV-2 in wastewater is in the range of 4.8 and 7.2 h [44] , while another study reported that the virus causing COVID-19 could survive in untreated wastewater from hours to days [59] . It is essential to understand the stability and decay mechanism of SARS-CoV-2 in water and wastewater. The significant parameters that can predict the reduction kinetics need to be determined, to establish the favorable and unfavorable conditions for the survival of SARS-CoV-2 in aqueous environment. Detailed investigations of various water/wastewater samples with different characteristics are required to assess the potential exposure risk of water contaminated with this virus. Launching a monitoring program is vital to determine the fate of SARS-CoV-2 water cycle [49] . Theoretical and computational analysis might help to provide a basis for experimental research.

Until now, there is no evidence and enough data to confirm if the water and wastewater containing SARS-CoV-2 could be the potential source of its transmission. Although some studies predicted a low risk of SARS-CoV-2 transmission via wastewater (especially the treated water) [55, 60] , still extensive investigations need to be performed to validate these predictions and inial findings. The information regarding the viability of the closely related SARS-CoV-1 virus in wastewater may provide useful information about the survival of SARS-CoV-2 in an aqueous environment [54] .

As a precaution, the wastewater/sewage treatment plants should be considered as the potential transmission routes. A recent study demonstrated the quantitative microbial risk assessment (QMRA) approach to investigate the potential health risks of SARS-CoV-2 in sewage to WWTPs workers [61] . Results revealed that the viral loads in wastewater/sewage at the entrance of WWTPs were above the WHO benchmark of tolerable risk used for virus infection of 10 -3 [6, 7, 14, 16, 52, 53] . It is essential to evaluate bioaerosol and airborne particle risks for nearby communities and WWTP workers [62] . In general, it can be made mandatory for the WWTP workers that perform manual cleaning of screening use face masks and face shields.

The aerosol formation during wastewater treatment needs to be carefully examined as a potential source of virus transmission [62] . Water-related exposures may occur in communities with weak sewage infrastructure, or that use wastewater for irrigation [63] . Viral aerosols from a leaking sewage pipe at Amoy Gardens complex in Hong Kong were identified as the transmission route for coronaviruses during the SARS-CoV-1 outbreak in 2003 [47] .

The virus can also make its way into the drinking water distribution systems from the accidental contamination of drinking water with raw sewage and can enter individual homes. The presence J o u r n a l P r e -p r o o f of SARS-CoV-2 in water may also affect the water supply system by forming the biofilms that can compromise the water quality and endanger human health.

There is also an imperative need for research to investigate the fecal-oral transmission potential of SARS-CoV-2 [33, 64] . It is crucial to examine if the SARS-CoV-2-contaminated wastewater has adverse impacts on aquatic life, soil, and wildlife. This needs careful examinations, risk analysis and thorough investigations to reach a conclusion. Furthermore, monitoring and control measures are essential along the wastewater treatment route to avert coronavirus spread.

It is recommended that the WWTP managers and stakeholders develop risk management strategies for the protection of WWTP workers and nearby communities [61] . The disinfection steps in both WWTPs and drinking water plants need to be developed carefully. The quantitative disinfection kinetics need to be established, and an optimum dose for inactivation of SARS-CoV-2 need to be determined. Decentralized virus inactivation treatment for waster discharged from WWTPs and drinking water plants can also be beneficial in reducing the environmental load of SARS-CoV-2.

Detection and quantification of SARS-CoV-2 in water and wastewater is an essential but challenging step to track the infectious disease [50, 65] . Due to complex nature of wastewater matrix, it is essential to develop new biomarker extraction techniques as well as selective, sensitive, and cost-effective tools for the analysis of wastewater samples containing the novel virus [66] . To avoid exposure to dangerous virus SARS-CoV-2 and to protect the safety of the lab analysts, it is recommended to use virus surrogates instead of harmful SARS-CoV-2 [34] .

Wastewater-based epidemiology (WBE) has been recognized as a useful tool for evaluating, predicting and managing the disease outbreaks [40, 43, 44, 67] . The concept is mainly based upon the detection, extraction, and analysis of biological and chemical compounds (referred to as biomarkers). Recent studies have reported the successful detection of the novel virus in municipal wastewater and human stool [6, 7, 15, 42, 51, [68] [69] [70] . Surveillance of relative changes in concentrations of SARS-CoV-2 RNA at the inlet of WWTP over time can serve as a useful tool for early warning for virus spread in the population [19] . Both upstream sampling (i.e., at sewerage maintenance holes) and downstream sampling (i.e., at the WWTP) approach can be used; however, upstream sampling is more appropriate due to variability in downstream samples.

A GIS-based sewerage map and flow rates would aid in the selection of upstream sampling locations [71] . Quantification of SARS-CoV-2 RNA in wastewater settled solids in WWTPs may also be used as a reliable and sensitive target for WBE [72] .

Moreover, monitoring of international airports and seaports sewage would allow very early detection of the entrance of the virus into a country. It could also help the relevant authorities in deciding the implementation or removal of lockdown [34] . It is essential to develop an integrated wastewater surveillance programs by considering both privacy and inequality concerns of the public [71] .

Wurtzer et al. [73] performed the time-course quantitative analysis of SARS-CoV-2 in Paris sewage by RT-qPCR to investigate virus circulation in humans. However, these studies are at the initial stage, and a large pool of data from different parts of the world is necessary to develop a reliable and sensitive detection method for SARS-CoV-2 detection in wastewater/sewage [74] .

A multidisciplinary research approach, including engineers, microbiologists, chemists, and public health experts, will be productive to nurture more effective techniques for the quantification of SARS-CoV-2 in water. Since SARS-CoV-2 has a presumably short half-life in water, the detection technique must be valid for both viable and non-viable SARS-CoV-2 in water. The virus can be degraded into other products; it is essential to develop strategies that could use these degraded products as target materials for detection and quantification [50] .

The lack of standardized and optimized protocol for the detection and quantification of SARS-CoV-2 in wastewater is another major challenge [59, 75, 76] . This may lead to discrepancies in the results obtained by different laboratories, as indicated by a recent study [3] . Currently, the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) has been employed widely for detection of SARS-CoV-2 in water samples [77] .

There is an imperative need to develop a standard operating procedure for accurate detection and quantification SARS-CoV-2 in water and wastewater. Furthermore, a standard sampling procedure must be developed to extract/isolate, detect and quantify the virus accurately. This is an essential step for the development of commercial laboratories in various parts of the world that can accurately detect and quantify the virus with reproducible results.

Advancements in the field of microbiology might play a key role in developing a low cost, efficient method for detection and quantification of viral RNA [78] . Recently, various innovative detection techniques have been reported in the literature [79] [80] [81] . Novel nanomaterials-based sensors were useful for the detection of waterborne pathogens [82] . There is a research potential to develop techniques using a similar approach for the detection of SARS-CoV-2 by utilizing J o u r n a l P r e -p r o o f different novel nanomaterials. A reliable widely-accepted surveillance system needs to be developed for the accurate quantification of SARS-CoV-2 in water samples.

The persistence of SARS-CoV-2 in the water environment is assumed to be short due to the enveloped nature of the virus [49, 60, 83] . The half-life of SARS-CoV-2 in hospital wastewater is in the range of 4.8 and 7.2 h at 20 °C [44] . However, little is known about the vitality of SARS-CoV-2 in water [16] . Furthermore, the accurate survival period and concentration of SARS-CoV-2 in water are still indefinite, and these are open areas for research.

It is essential to understand the decay mechanism and breakdown products of SARS-CoV-2 in water and wastewater. It will not only help in developing effective techniques for accurate detection and quantification but also will be useful for proposing an efficient disinfection technique. A recent study reported the transcriptomic architecture of SARS-CoV-2 [84] . The SARS-CoV-2 may present in the water phase either in a viable state or in the non-viable form of viral debris [50] . However, the actual degradation of products and accurate decay mechanism of SARS-CoV-2 in water phase need to be explored yet.

The traditional treatment techniques for the removal of viruses from wastewater include sand filters, chlorine treatment, UV inactivation, ozone treatment, microbial treatment, membranes and pond systems [85] [86] [87] [88] [89] [90] . Though it is assumed that the current techniques used for wastewater treatment might be helpful to eliminate the novel virus; due to the global impact of this J o u r n a l P r e -p r o o f pandemic, experimental results are needed to validate this assumption [62, [91] [92] [93] . Likewise, experimental shreds of evidence are required to confirm the effectiveness of household filtration systems, chlorination, densification and boiling for the elimination of the novel virus from water.

It might be needed to upgrade the existing water and wastewater treatment systems or develop new treatment techniques to treat the SARS-CoV-2-contaminated water. The more stringent treatment above the current level is needed for virus reduction to ensure the safety of recycled water [94, 95] . WHO has highlighted guidelines on the safe management of water in July 2020 [62] . A recent study stated that the current disinfection strategy recommended by WHO might not be adequate to deactivate SARS-CoV-2 in water [96] . The existing wastewater treatment system might need necessary alternation or additional pretreatment steps to deactivate the SARS-CoV-2. It should also be investigated if the presence of SARS-CoV-2 in wastewater affect the treatment process for the removal of other pollutants.

Recent advancement in nanotechnology, biotechnology and material sciences have opened many doors of applications and exhibited tremendous potential in water purification [97] [98] [99] [100] [101] [102] . The development of effective techniques for the inactivation of SARS-CoV-2 is vital to limit its presence in wastewater and lessen its potential adverse effects on human health and the environment. Significant treatment steps are essential to upgrade both the wastewater and drinking treatment pants to eradicate SARS-CoV-2 or its RNA fragments.

Recent years have witnessed tremendous progress in the applications of various novel materials in water treatment [103] [104] [105] [106] . Nanomaterials and their composites might play a critical role in the development of an efficient method for the eradication of SARS-CoV-2 from water [106] [107] [108] [109] [110] . Advances in membranes systems provide an effective route for the removal of viruses from water [111] [112] [113] [114] [115] .

For drinking water, a potable water straw or UV based system can be developed to filter/kill the virus and to reduce the risk of waterborne viral infection [116] . A recent study reported that there is a negative correlation between sunlight UV dose and percent positive of SARS-CoV-2 [117] .

Other techniques such as oxidation, coagulation and photocatalytic killing of the virus must also be explored to determine the effective treatment option for the deactivation of SARS-CoV-2 in water.

The efficiency of emerging disinfection technologies for SARS-CoV-2 inactivation need to be appraised. The water distribution systems may potentially host the novel virus due to biofilm growth and presence of bacterial colonies. Innovations and improvements in the water distribution and plumbing systems are vital to minimize the transmission of SARS-CoV-2 through the water [118] . As a general rule, it is the combination of different techniques in primary, secondary and tertiary treatments that would allow enough virus removal to limit the possibility of environmental contamination. The SARS-CoV-2 pandemic should develop a global awareness that solutions have to be found for improving the wastewater treatment even in developing countries, due to the possibility of its spread through the aquatic environment from inadequately treated wastewater. The presence of SARS-CoV-2 in wastewater may have consequences for public health in developing countries with poor water and sewage infrastructure, and inadequate institutional treatment/disinfection facilities.

Future research in these lines is essential for the protection of public health and the environment.

In addition, robust policy intervention is essential to ensure reasonable compliance regarding the discharge of wastewater in various parts of the world.

J o u r n a l P r e -p r o o f One of the major environmental concern associated with COVID-19 pandemic is the excessive use of disinfectants (such as alcohol-based hand sanitizers and disinfecting soaps). The usage of disinfectants during the COVID-19 pandemic increases environment and energy footprints significantly. It is important to carefully monitor the wastewater for possible commination and mitigate the spread of SARS-CoV-2. A recent report reveals that 2000 tons of disinfectants are dispensed at in sewage systems in Wuhan City of China from January 29 to February 18, 2020 [119, 120] . These discharges not only poses a potential threat to the marine environment but can also contaminate drinking water resources. Another study estimated that excessive use of disinfectants and frequent handwashing could increase consumption of drinking water by 20% and lead to the generation of 15-18% more wastewater [121] .

The alcohol-based hand sanitizer is not only associated with some health issues, but it may also result in adverse impacts on the environment [122, 123] . A recent study highlighted that the extensive use of disinfectants poses a significant threat to urban wildlife [124] . These disinfectants may find their ways to reach water bodies and pollute them. It is, therefore, necessary to assess the exact hostile impacts of the disinfectants that discharge into water bodies and mitigate them to the maximum possible extent. In addition, the use of masks, gloves and other personal protective equipment (PPE) resulted in the generation of a massive amount of wastes in the environment. Strategies for proper management of waste must be developed to lessen its adverse environmental impacts.

In summary, besides severe threats to humanity, the ongoing pandemic offers the opportunity to environmental engineers and scientists to play a responsible role to control the potential spread Multidisciplinary research, including engineers, microbiologists, chemists, and public health experts are needed to address these challenges and to safeguard the aquatic environments. Figures   Fig. 1 . Potential challenges and opportunities in the field of water research 

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Postlockdown detection of SARS-CoV-2 RNA in the wastewater of Montpellier, France, One Heal

The authors declare that there is no conflict of interest during and after the study.The authors declare that there is no conflict of interest regarding the publication of this article J o u r n a l P r e -p r o o f

(February-April) 2020 Wastewater from WWTPsMilan and Rome (Italy) [17] April 2020 Wastewater from WWTPs Istanbul (Turkey) [18] June 2020 River water Quito (Ecuador) [20] (April-May) 2020 Aircraft and cruise ship wastewater Passenger aircraft flight from Los Angeles -Brisbane Hongkong -Brisbane New Delhi-Sydney Cruise ship (Australia) [22] March 2020Wastewater from the treatment facility Massachusetts (USA) [42] (February-March) 2020Sewage/Wastewater from WWTPs Netherlands [51] May 2020 Wastewater from WWTPs Gujarat (India) [53] April 2020Raw and treated wastewater samples from WWTPsMilano Metropolitan Area Italy) [60] (March-April) 2020 Wastewater from WWTPs Paris (France) [73] February 2020 Sewage from hospital sewage disinfection pool Hospital of Zhejiang University, China [92] May 2020 Wastewater from WWTPs, influent pump stations, or interceptor lines New York (USA) [125] (March-April) 2020 Wastewater from WWTPs Different localities in Israel [126] (February-April) 2020 Wastewater from WWTPs Valencia (Spain) [127] (March-May) 2020 Secondary-treated wastewater Yamanashi Prefecture (Japan) [128] (March-April) 2020 Wastewater from WWTPs Ishikawa and Toyama prefectures (Japan) [129] April 2020 Wastewater from WWTPs Ourense (Spain) [130] (May-June) 2020 Wastewater from WWTPs Jaipur (India) [131] (March-April) 2020Wastewater from the drainage of COVID-19 infected areas and quarantine centerVarious districts in Pakistan [132] (January-April) 2020 Wastewater from WWTPs Southern Louisiana (USA) [133] (April-June 2020) Untreated wastewater from WWTPs Czech Republic [134] April 2020 Wastewater from WWTPs North-Rhine Westphalia (Germany) [135] (May-July) 2020 Wastewater from WWTPs Montpellier (France) [136]