id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-004020-qtwcbn7m	Gao, Yaning	Identification of Novel Natural Products as Effective and Broad-Spectrum Anti-Zika Virus Inhibitors	2019-11-02		.txt	text/plain	7693	354	50	A combination of gossypol with any of the three natural products identified in this study, as well as with bortezomib, a previously reported anti-ZIKV compound, exhibited significant combinatorial inhibitory effects against three ZIKV human strains tested. Gossypol-treated ZIKV was incubated with Vero E6 cells at 37 • C for 1 h in the presence of DMEM containing serial dilutions of each of the other three natural products identified, such as curcumin, digitonin, and conessine, or anti-ZIKV compound control (bortezomib). Based on Table 1 , four "hit" natural products, including gossypol, curcumin, digitonin, and conessine ( Figure 2A -D), were selected, since they demonstrated inhibitory activity against ZIKV infection with no obvious cytotoxicity in Vero E6 cells when observed under a microscope. Since gossypol demonstrated the highest antiviral activity individually against all ZIKV strains tested, we next investigated the potential combinatorial effects of the combination of gossypol with three other natural products identified, namely curcumin, digitonin, and conessine, as well as anti-ZIKV compound control (bortezomib).	./cache/cord-004020-qtwcbn7m.txt	./txt/cord-004020-qtwcbn7m.txt