id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-253616-7jyui5ca	Lai, Zheng-Zong	Harringtonine Inhibits Zika Virus Infection through Multiple Mechanisms	2020-09-07		.txt	text/plain	4969	286	48	To investigate the anti-ZIKV activity of harringtonine, we assessed the inhibition of virus infection in Vero cells with MOI = 0.01 under different concentrations of harringtonine for 48 h. Intracellular viral RNA levels, protein expression levels and virus progeny in supernatants were respectively determined by RT-qPCR, western blotting and fluorescent focus assay (FFA). The dose-dependent anti-ZIKV activities of harringtonine were observed to decrease viral RNA/protein production and progeny yield ( Figure 1B-D) , indicating that virus propagation was suppressed. Harringtonine (625 nM) was administered at different stages of ZIKV infection with MOI = 0.1, and then the levels of ZIKV RNA in cells, as well as virus titers in supernatants, were determined after 24 h treatment. Harringtonine (625 nM) was administered at different stages of ZIKV infection with MOI = 0.1, and then the levels of ZIKV RNA in cells, as well as virus titers in supernatants, were determined after 24 h treatment.	./cache/cord-253616-7jyui5ca.txt	./txt/cord-253616-7jyui5ca.txt