id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
cord-309275-soffxxqu	Li, Shuang	DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1	2020-10-17		.txt	text/plain	3962	223	52	title: DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1 KEY FINDINGS: Through human transcriptome array (HTA) we found the defensin alpha 1B (DEFA1B) expression was significantly increased within exosomes isolated from ZIKV infected A549 cells. Further studies demonstrated that DEFA1B interacted with the origin recognition complex 1 (ORC1) which is required to initiate DNA replication during the cell cycle and increased DEFA1B expression decreased the ORC1 level in the cell nuclei. SIGNIFICANCE: Together, our results demonstrated that the anti-ZIKV activity of DEFA1B can be mediated by exosomes, and DEFA1B interacts with ORC1 to retard cell cycles. Both qPCR ( Figure 3K ) and western blot ( Figure 3L ) data showed no significant changes of ZIKV replication between untreated HEK293T cells and cells with up-regulated DEFA1B group. Our data showed the ZIKV induced exosomes could be internalized into recipient cells and inhibit the cells' DNA replication to retard cell cycles.	./cache/cord-309275-soffxxqu.txt	./txt/cord-309275-soffxxqu.txt