id	author	title	date	pages	extension	mime	words	sentences	flesch	summary	cache	txt
work_loeb6buttvevvfbcegmk472amm	R. Max Wynn	Biochemical Basis of Type IB (E1β) Mutations in Maple Syrup Urine Disease	2001	7	.pdf	application/pdf	7353	600	69	We have recently studied MSUD mutations in Israeli patients, in particular those from the non-Jewish Druze kindred. novel type IB mutation apparently disrupts �/�� subunit interactions, resulting in the formation of inactive E1 heterodimers, Temperature-dependent Folding and Assembly of Mutant E1—Cultures (1 liter in size) for the expression of His6-tagged wild-type and BCKD complex (20) was used to determine wild-type and mutant E1 Thermal Inactivation of Wild-type and MSUD Mutant E1 Proteins— 1. Elution profiles of wild-type and MSUD mutant E1 from Levels of both the wild-type E1� and the mutant E1� subunits expression of wild-type and mutant E1, followed by an overnight incubation at indicated temperatures. 4. Thermal inactivation of wild-type and MSUD mutant Wild-type and MSUD mutant E1 proteins at 32 Kinetic constants (kcat and Km) for wild-type and MSUD mutant E1 5. Denaturation of wild-type and mutant E1 proteins in Free energy changes for wild-type and MSUD mutant E1 in GdnHCl	./cache/work_loeb6buttvevvfbcegmk472amm.pdf	./txt/work_loeb6buttvevvfbcegmk472amm.txt