id author title date pages extension mime words sentences flesch summary cache txt 10_1101-2021_02_10_430656 Zakeri, Mohsen A like-for-like comparison of lightweight-mapping pipelines for single-cell RNA-seq data pre-processing 2021 7 .pdf application/pdf 6557 568 64 A like-for-like comparison of lightweight-mapping pipelines for single-cell RNA-seq data pre-processing benchmark comparing the kallisto-bustools pipeline (2) for single-cell demonstrate that, when configured to match the computational complexity of kallisto-bustools as closely as possible, alevin-fry processes Alevin-fry (3) is a new pipeline for single-cell RNA-seq benchmarking STARsolo (9), kallisto-bustools (2) and alevin-fry (3), out new tools like alevin-fry for the pre-processing of single-cell data, (1), we have now created a simple-to-follow tutorial for speedoptimized single-cell pre-processing using alevin-fry (https:// by Booeshaghi and Pachter (1) change when a like-for-like comparison between alevin-fry and kallisto-bustools is carried out, we The time and memory used by the relevant steps of the alevin-fry and kallisto-bustools pipelines for pre-processing the 20 diverse tagged-end single-cell RNA-seq datasets used in (1). A comparison of the resulting count matrices obtained from alevin-fry and kallisto-bustools, as run in this manuscript, for the pbmc_10k_v3 dataset. peak memory than alevin-fry, with the kallisto-bustools pipeline using ./cache/10_1101-2021_02_10_430656.pdf ./txt/10_1101-2021_02_10_430656.txt