id sid tid token lemma pos pk02c823v13 1 1 the the DET pk02c823v13 1 2 objective objective NOUN pk02c823v13 1 3 of of ADP pk02c823v13 1 4 my my PRON pk02c823v13 1 5 first first ADJ pk02c823v13 1 6 project project NOUN pk02c823v13 1 7 was be AUX pk02c823v13 1 8 to to PART pk02c823v13 1 9 investigate investigate VERB pk02c823v13 1 10 the the DET pk02c823v13 1 11 interaction interaction NOUN pk02c823v13 1 12 of of ADP pk02c823v13 1 13 a a DET pk02c823v13 1 14 class class NOUN pk02c823v13 1 15 d d NOUN pk02c823v13 1 16 beta beta NOUN pk02c823v13 1 17 - - PUNCT pk02c823v13 1 18 lactamase lactamase NOUN pk02c823v13 1 19 with with ADP pk02c823v13 1 20 different different ADJ pk02c823v13 1 21 beta beta NOUN pk02c823v13 1 22 - - PUNCT pk02c823v13 1 23 lactams lactam NOUN pk02c823v13 1 24 to to PART pk02c823v13 1 25 explore explore VERB pk02c823v13 1 26 if if SCONJ pk02c823v13 1 27 this this DET pk02c823v13 1 28 family family NOUN pk02c823v13 1 29 of of ADP pk02c823v13 1 30 enzymes enzyme NOUN pk02c823v13 1 31 is be AUX pk02c823v13 1 32 diffusion diffusion NOUN pk02c823v13 1 33 - - PUNCT pk02c823v13 1 34 limited limited ADJ pk02c823v13 1 35 or or CCONJ pk02c823v13 1 36 not not PART pk02c823v13 1 37 . . PUNCT pk02c823v13 2 1 this this DET pk02c823v13 2 2 project project NOUN pk02c823v13 2 3 required require VERB pk02c823v13 2 4 isolation isolation NOUN pk02c823v13 2 5 and and CCONJ pk02c823v13 2 6 purification purification NOUN pk02c823v13 2 7 of of ADP pk02c823v13 2 8 the the DET pk02c823v13 2 9 oxa-10 oxa-10 PROPN pk02c823v13 2 10 beta beta NOUN pk02c823v13 2 11 - - PUNCT pk02c823v13 2 12 lactamase lactamase NOUN pk02c823v13 2 13 overexpressed overexpresse VERB pk02c823v13 2 14 in in ADP pk02c823v13 2 15 e. e. NOUN pk02c823v13 2 16 coli coli NOUN pk02c823v13 2 17 bl21 bl21 PROPN pk02c823v13 2 18 and and CCONJ pk02c823v13 2 19 the the DET pk02c823v13 2 20 determination determination NOUN pk02c823v13 2 21 of of ADP pk02c823v13 2 22 kcat kcat PROPN pk02c823v13 2 23 , , PUNCT pk02c823v13 2 24 km km PROPN pk02c823v13 2 25 , , PUNCT pk02c823v13 2 26 and and CCONJ pk02c823v13 2 27 vmax vmax PROPN pk02c823v13 2 28 parameters parameter NOUN pk02c823v13 2 29 for for ADP pk02c823v13 2 30 different different ADJ pk02c823v13 2 31 beta beta NOUN pk02c823v13 2 32 - - PUNCT pk02c823v13 2 33 lactam lactam NOUN pk02c823v13 2 34 antibiotics antibiotic NOUN pk02c823v13 2 35 at at ADP pk02c823v13 2 36 different different ADJ pk02c823v13 2 37 viscosities viscosity NOUN pk02c823v13 2 38 of of ADP pk02c823v13 2 39 the the DET pk02c823v13 2 40 reaction reaction NOUN pk02c823v13 2 41 buffer buffer NOUN pk02c823v13 2 42 . . PUNCT pk02c823v13 3 1 the the DET pk02c823v13 3 2 other other ADJ pk02c823v13 3 3 project project NOUN pk02c823v13 3 4 that that PRON pk02c823v13 3 5 i i PRON pk02c823v13 3 6 worked work VERB pk02c823v13 3 7 on on ADP pk02c823v13 3 8 required require VERB pk02c823v13 3 9 the the DET pk02c823v13 3 10 cloning cloning NOUN pk02c823v13 3 11 of of ADP pk02c823v13 3 12 blai blai PROPN pk02c823v13 3 13 gene gene NOUN pk02c823v13 3 14 into into ADP pk02c823v13 3 15 an an DET pk02c823v13 3 16 expression expression NOUN pk02c823v13 3 17 vector vector NOUN pk02c823v13 3 18 , , PUNCT pk02c823v13 3 19 overexpression overexpression NOUN pk02c823v13 3 20 of of ADP pk02c823v13 3 21 blai blai PROPN pk02c823v13 3 22 in in ADP pk02c823v13 3 23 e. e. PROPN pk02c823v13 3 24 coli coli PROPN pk02c823v13 3 25 bl21 bl21 PROPN pk02c823v13 3 26 , , PUNCT pk02c823v13 3 27 purification purification NOUN pk02c823v13 3 28 of of ADP pk02c823v13 3 29 this this DET pk02c823v13 3 30 protein protein NOUN pk02c823v13 3 31 . . PUNCT pk02c823v13 4 1 this this DET pk02c823v13 4 2 protein protein NOUN pk02c823v13 4 3 is be AUX pk02c823v13 4 4 a a DET pk02c823v13 4 5 gene gene NOUN pk02c823v13 4 6 repressor repressor NOUN pk02c823v13 4 7 , , PUNCT pk02c823v13 4 8 believed believe VERB pk02c823v13 4 9 to to PART pk02c823v13 4 10 be be AUX pk02c823v13 4 11 involved involve VERB pk02c823v13 4 12 in in ADP pk02c823v13 4 13 inducible inducible ADJ pk02c823v13 4 14 expression expression NOUN pk02c823v13 4 15 of of ADP pk02c823v13 4 16 beta beta NOUN pk02c823v13 4 17 - - PUNCT pk02c823v13 4 18 lactamases lactamase NOUN pk02c823v13 4 19 in in ADP pk02c823v13 4 20 s. s. PROPN pk02c823v13 4 21 aureus aureus PROPN pk02c823v13 4 22 . . PUNCT