id sid tid token lemma pos w9504x54149 1 1 in in ADP w9504x54149 1 2 - - PUNCT w9504x54149 1 3 situ situ NOUN w9504x54149 1 4 sensing sensing NOUN w9504x54149 1 5 of of ADP w9504x54149 1 6 pathogens pathogen NOUN w9504x54149 1 7 of of ADP w9504x54149 1 8 concern concern NOUN w9504x54149 1 9 in in ADP w9504x54149 1 10 waters water NOUN w9504x54149 1 11 is be AUX w9504x54149 1 12 a a DET w9504x54149 1 13 need need NOUN w9504x54149 1 14 for for ADP w9504x54149 1 15 water water NOUN w9504x54149 1 16 monitoring monitoring NOUN w9504x54149 1 17 agencies agency NOUN w9504x54149 1 18 . . PUNCT w9504x54149 2 1 the the DET w9504x54149 2 2 unpredictable unpredictable ADJ w9504x54149 2 3 , , PUNCT w9504x54149 2 4 rapid rapid ADJ w9504x54149 2 5 fluctuations fluctuation NOUN w9504x54149 2 6 in in ADP w9504x54149 2 7 indicator indicator NOUN w9504x54149 2 8 organism organism NOUN w9504x54149 2 9 ( ( PUNCT w9504x54149 2 10 escherichia escherichia PROPN w9504x54149 2 11 coliì¢ coliì¢ PROPN w9504x54149 2 12 ââ ââ PROPN w9504x54149 2 13 ' ' PUNCT w9504x54149 2 14 ec ec PROPN w9504x54149 2 15 ) ) PUNCT w9504x54149 2 16 concentrations concentration NOUN w9504x54149 2 17 renders render VERB w9504x54149 2 18 current current ADJ w9504x54149 2 19 standards standard NOUN w9504x54149 2 20 and and CCONJ w9504x54149 2 21 methods method NOUN w9504x54149 2 22 both both CCONJ w9504x54149 2 23 untimely untimely ADV w9504x54149 2 24 and and CCONJ w9504x54149 2 25 unrepresentative unrepresentative ADJ w9504x54149 2 26 of of ADP w9504x54149 2 27 the the DET w9504x54149 2 28 water water NOUN w9504x54149 2 29 body body NOUN w9504x54149 2 30 as as ADP w9504x54149 2 31 a a DET w9504x54149 2 32 whole whole NOUN w9504x54149 2 33 . . PUNCT w9504x54149 3 1 the the DET w9504x54149 3 2 use use NOUN w9504x54149 3 3 of of ADP w9504x54149 3 4 glucuronides glucuronide NOUN w9504x54149 3 5 with with ADP w9504x54149 3 6 a a DET w9504x54149 3 7 fluorescent fluorescent NOUN w9504x54149 3 8 label label NOUN w9504x54149 3 9 allows allow VERB w9504x54149 3 10 ec ec PROPN w9504x54149 3 11 detection detection NOUN w9504x54149 3 12 by by ADP w9504x54149 3 13 identifying identify VERB w9504x54149 3 14 ìä ìä PROPN w9504x54149 3 15 ü ü NOUN w9504x54149 3 16 - - NOUN w9504x54149 3 17 glucuronidase glucuronidase NOUN w9504x54149 3 18 ( ( PUNCT w9504x54149 3 19 gus gus PROPN w9504x54149 3 20 ) ) PUNCT w9504x54149 3 21 expressed express VERB w9504x54149 3 22 through through ADP w9504x54149 3 23 ec ec PROPN w9504x54149 3 24 's 's PART w9504x54149 3 25 natural natural ADJ w9504x54149 3 26 metabolic metabolic NOUN w9504x54149 3 27 processes process NOUN w9504x54149 3 28 . . PUNCT w9504x54149 4 1 this this DET w9504x54149 4 2 activity activity NOUN w9504x54149 4 3 can can AUX w9504x54149 4 4 be be AUX w9504x54149 4 5 quantified quantify VERB w9504x54149 4 6 in in ADP w9504x54149 4 7 a a DET w9504x54149 4 8 stop stop VERB w9504x54149 4 9 - - PUNCT w9504x54149 4 10 flow flow NOUN w9504x54149 4 11 sensor sensor NOUN w9504x54149 4 12 and and CCONJ w9504x54149 4 13 correlated correlate VERB w9504x54149 4 14 via via ADP w9504x54149 4 15 a a DET w9504x54149 4 16 quantification quantification NOUN w9504x54149 4 17 curve curve NOUN w9504x54149 4 18 back back ADV w9504x54149 4 19 to to ADP w9504x54149 4 20 initial initial ADJ w9504x54149 4 21 ec ec PROPN w9504x54149 4 22 concentrations concentration NOUN w9504x54149 4 23 . . PUNCT w9504x54149 5 1 a a DET w9504x54149 5 2 fluorescent fluorescent NOUN w9504x54149 5 3 labeled label VERB w9504x54149 5 4 glucuronide glucuronide NOUN w9504x54149 5 5 that that PRON w9504x54149 5 6 is be AUX w9504x54149 5 7 soluble soluble ADJ w9504x54149 5 8 and and CCONJ w9504x54149 5 9 highly highly ADV w9504x54149 5 10 fluorescent fluorescent ADJ w9504x54149 5 11 at at ADP w9504x54149 5 12 natural natural ADJ w9504x54149 5 13 water water NOUN w9504x54149 5 14 phs phs NOUN w9504x54149 5 15 is be AUX w9504x54149 5 16 also also ADV w9504x54149 5 17 necessary necessary ADJ w9504x54149 5 18 . . PUNCT w9504x54149 6 1 the the DET w9504x54149 6 2 current current ADJ w9504x54149 6 3 lack lack NOUN w9504x54149 6 4 of of ADP w9504x54149 6 5 understanding understanding NOUN w9504x54149 6 6 of of ADP w9504x54149 6 7 the the DET w9504x54149 6 8 factors factor NOUN w9504x54149 6 9 that that PRON w9504x54149 6 10 influence influence VERB w9504x54149 6 11 gus gus NOUN w9504x54149 6 12 synthesis synthesis NOUN w9504x54149 6 13 and and CCONJ w9504x54149 6 14 expression expression NOUN w9504x54149 6 15 in in ADP w9504x54149 6 16 natural natural ADJ w9504x54149 6 17 waters water NOUN w9504x54149 6 18 is be AUX w9504x54149 6 19 addressed address VERB w9504x54149 6 20 in in ADP w9504x54149 6 21 this this DET w9504x54149 6 22 dissertation dissertation NOUN w9504x54149 6 23 . . PUNCT w9504x54149 7 1 the the DET w9504x54149 7 2 information information NOUN w9504x54149 7 3 presented present VERB w9504x54149 7 4 here here ADV w9504x54149 7 5 increases increase VERB w9504x54149 7 6 the the DET w9504x54149 7 7 accuracy accuracy NOUN w9504x54149 7 8 of of ADP w9504x54149 7 9 an an DET w9504x54149 7 10 in in ADP w9504x54149 7 11 - - PUNCT w9504x54149 7 12 situ situ NOUN w9504x54149 7 13 optical optical ADJ w9504x54149 7 14 sensor sensor NOUN w9504x54149 7 15 . . PUNCT w9504x54149 8 1 this this DET w9504x54149 8 2 dissertation dissertation NOUN w9504x54149 8 3 reports report VERB w9504x54149 8 4 for for ADP w9504x54149 8 5 the the DET w9504x54149 8 6 first first ADJ w9504x54149 8 7 time time NOUN w9504x54149 8 8 the the DET w9504x54149 8 9 michaelis michaelis ADJ w9504x54149 8 10 - - ADJ w9504x54149 8 11 menten menten PROPN w9504x54149 8 12 kinetic kinetic ADJ w9504x54149 8 13 parameters parameter NOUN w9504x54149 8 14 of of ADP w9504x54149 8 15 gus gus NOUN w9504x54149 8 16 using use VERB w9504x54149 8 17 the the DET w9504x54149 8 18 substrate substrate NOUN w9504x54149 8 19 carboxyumbelliferyl carboxyumbelliferyl NOUN w9504x54149 8 20 - - PUNCT w9504x54149 8 21 ìä ìä PROPN w9504x54149 8 22 ü ü PROPN w9504x54149 8 23 - - NOUN w9504x54149 8 24 d d NOUN w9504x54149 8 25 - - ADJ w9504x54149 8 26 glucuronide glucuronide PROPN w9504x54149 8 27 ( ( PUNCT w9504x54149 8 28 cug cug NOUN w9504x54149 8 29 ) ) PUNCT w9504x54149 8 30 as as ADP w9504x54149 8 31 km=0.910 km=0.910 PROPN w9504x54149 8 32 , , PUNCT w9504x54149 8 33 vmax vmax PROPN w9504x54149 8 34 = = PROPN w9504x54149 8 35 0.041 0.041 NUM w9504x54149 8 36 , , PUNCT w9504x54149 8 37 vmax vmax PROPN w9504x54149 8 38 / / SYM w9504x54149 8 39 km km PROPN w9504x54149 8 40 0.045 0.045 NUM w9504x54149 8 41 mm mm PROPN w9504x54149 8 42 min-1 min-1 SPACE w9504x54149 8 43 ; ; PUNCT w9504x54149 8 44 optimal optimal ADJ w9504x54149 8 45 ph ph NOUN w9504x54149 8 46 is be AUX w9504x54149 8 47 6.5ìâå±1.0 6.5ìâå±1.0 NUM w9504x54149 8 48 ; ; PUNCT w9504x54149 8 49 optimal optimal ADJ w9504x54149 8 50 temperature temperature NOUN w9504x54149 8 51 is be AUX w9504x54149 8 52 38ìü 38ìü ADJ w9504x54149 8 53 ác ác NOUN w9504x54149 8 54 ; ; PUNCT w9504x54149 8 55 cug cug NOUN w9504x54149 8 56 hydrolysis hydrolysis NOUN w9504x54149 8 57 is be AUX w9504x54149 8 58 not not PART w9504x54149 8 59 significantly significantly ADV w9504x54149 8 60 affected affect VERB w9504x54149 8 61 by by ADP w9504x54149 8 62 heavy heavy ADJ w9504x54149 8 63 solvents solvent NOUN w9504x54149 8 64 suggesting suggest VERB w9504x54149 8 65 proton proton NOUN w9504x54149 8 66 transfer transfer NOUN w9504x54149 8 67 and and CCONJ w9504x54149 8 68 solvent solvent NOUN w9504x54149 8 69 addition addition NOUN w9504x54149 8 70 that that PRON w9504x54149 8 71 occur occur VERB w9504x54149 8 72 during during ADP w9504x54149 8 73 hydrolysis hydrolysis NOUN w9504x54149 8 74 are be AUX w9504x54149 8 75 not not PART w9504x54149 8 76 limiting limit VERB w9504x54149 8 77 steps step NOUN w9504x54149 8 78 ; ; PUNCT w9504x54149 8 79 gus gus PROPN w9504x54149 8 80 preferentially preferentially ADV w9504x54149 8 81 binds bind VERB w9504x54149 8 82 to to ADP w9504x54149 8 83 mug mug NOUN w9504x54149 8 84 in in ADP w9504x54149 8 85 comparison comparison NOUN w9504x54149 8 86 to to ADP w9504x54149 8 87 cug cug PROPN w9504x54149 8 88 . . PUNCT w9504x54149 9 1 additionally additionally ADV w9504x54149 9 2 , , PUNCT w9504x54149 9 3 it it PRON w9504x54149 9 4 is be AUX w9504x54149 9 5 found find VERB w9504x54149 9 6 fluorescent fluorescent NOUN w9504x54149 9 7 reagents reagent NOUN w9504x54149 9 8 facilitate facilitate VERB w9504x54149 9 9 the the DET w9504x54149 9 10 induction induction NOUN w9504x54149 9 11 of of ADP w9504x54149 9 12 gus gus PROPN w9504x54149 9 13 in in ADP w9504x54149 9 14 ec ec PROPN w9504x54149 9 15 ; ; PUNCT w9504x54149 9 16 increases increase NOUN w9504x54149 9 17 in in ADP w9504x54149 9 18 gus gus NOUN w9504x54149 9 19 activity activity NOUN w9504x54149 9 20 per per ADP w9504x54149 9 21 culturable culturable ADJ w9504x54149 9 22 ec ec PROPN w9504x54149 9 23 cell cell NOUN w9504x54149 9 24 in in ADP w9504x54149 9 25 marine marine ADJ w9504x54149 9 26 water water NOUN w9504x54149 9 27 is be AUX w9504x54149 9 28 likely likely ADJ w9504x54149 9 29 due due ADJ w9504x54149 9 30 to to ADP w9504x54149 9 31 breakdown breakdown NOUN w9504x54149 9 32 of of ADP w9504x54149 9 33 cell cell NOUN w9504x54149 9 34 wall wall NOUN w9504x54149 9 35 and and CCONJ w9504x54149 9 36 release release NOUN w9504x54149 9 37 of of ADP w9504x54149 9 38 intracellular intracellular ADJ w9504x54149 9 39 enzymes enzyme NOUN w9504x54149 9 40 ; ; PUNCT w9504x54149 9 41 ec ec PROPN w9504x54149 9 42 gus gus PROPN w9504x54149 9 43 expression expression NOUN w9504x54149 9 44 in in ADP w9504x54149 9 45 natural natural ADJ w9504x54149 9 46 waters water NOUN w9504x54149 9 47 does do AUX w9504x54149 9 48 not not PART w9504x54149 9 49 significantly significantly ADV w9504x54149 9 50 correlate correlate VERB w9504x54149 9 51 to to ADP w9504x54149 9 52 initial initial ADJ w9504x54149 9 53 physiochemical physiochemical ADJ w9504x54149 9 54 water water NOUN w9504x54149 9 55 parameters parameter NOUN w9504x54149 9 56 or or CCONJ w9504x54149 9 57 ec ec PROPN w9504x54149 9 58 concentrations concentration NOUN w9504x54149 9 59 . . PUNCT w9504x54149 10 1 benefits benefit NOUN w9504x54149 10 2 of of ADP w9504x54149 10 3 this this DET w9504x54149 10 4 research research NOUN w9504x54149 10 5 include include VERB w9504x54149 10 6 a a DET w9504x54149 10 7 better well ADJ w9504x54149 10 8 understanding understanding NOUN w9504x54149 10 9 of of ADP w9504x54149 10 10 gus gus NOUN w9504x54149 10 11 production production NOUN w9504x54149 10 12 in in ADP w9504x54149 10 13 natural natural ADJ w9504x54149 10 14 waters water NOUN w9504x54149 10 15 ; ; PUNCT w9504x54149 10 16 a a DET w9504x54149 10 17 better well ADJ w9504x54149 10 18 understanding understanding NOUN w9504x54149 10 19 of of ADP w9504x54149 10 20 gus gus NOUN w9504x54149 10 21 expression expression NOUN w9504x54149 10 22 in in ADP w9504x54149 10 23 typical typical ADJ w9504x54149 10 24 sensor sensor NOUN w9504x54149 10 25 growing grow VERB w9504x54149 10 26 conditions condition NOUN w9504x54149 10 27 ; ; PUNCT w9504x54149 10 28 the the DET w9504x54149 10 29 characterization characterization NOUN w9504x54149 10 30 of of ADP w9504x54149 10 31 a a DET w9504x54149 10 32 soluble soluble ADJ w9504x54149 10 33 fluorescent fluorescent NOUN w9504x54149 10 34 reagent reagent ADJ w9504x54149 10 35 ideal ideal NOUN w9504x54149 10 36 for for ADP w9504x54149 10 37 in in ADP w9504x54149 10 38 - - PUNCT w9504x54149 10 39 situ situ NOUN w9504x54149 10 40 optical optical ADJ w9504x54149 10 41 sensing sensing NOUN w9504x54149 10 42 ; ; PUNCT w9504x54149 10 43 the the DET w9504x54149 10 44 use use NOUN w9504x54149 10 45 of of ADP w9504x54149 10 46 an an DET w9504x54149 10 47 optical optical ADJ w9504x54149 10 48 sensor sensor NOUN w9504x54149 10 49 for for ADP w9504x54149 10 50 the the DET w9504x54149 10 51 detection detection NOUN w9504x54149 10 52 and and CCONJ w9504x54149 10 53 partial partial ADJ w9504x54149 10 54 quantification quantification NOUN w9504x54149 10 55 of of ADP w9504x54149 10 56 pathogen pathogen PROPN w9504x54149 10 57 indicator indicator PROPN w9504x54149 10 58 organisms organism NOUN w9504x54149 10 59 . . PUNCT