Notre Dame scientists develop largest developmental proteomic data set for any animal | News | Notre Dame News | University of Notre Dame Skip To Content Skip To Navigation Skip To Search University of Notre Dame Notre Dame News Experts ND in the News Subscribe About Us Home Contact Search Menu Home › News › Notre Dame scientists develop largest developmental proteomic data set for any animal Notre Dame scientists develop largest developmental proteomic data set for any animal Published: March 26, 2014 Author: Gene Stowe and Marissa Gebhard Xenopus laevis, or African clawed frog Now that the human genome is sequenced, University of Notre Dame researchers are focusing on the study of the proteome, which is the protein content of an organism, tissue or cell. Bioanalytical chemist Norman Dovichi and molecular biologist Paul Huber have successfully tracked the changing patterns of protein expression during early development of Xenopus laevis, or African clawed frog, embryos. They have developed the largest data set on developmental proteomics for any organism, and have included the single-cell zygote. Their research has uncovered an unexpected amount of discordance between the levels of messenger RNA (mRNA) and its corresponding protein. Their findings are published in Scientific Reports in an article titled, “Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development.” The Notre Dame team based in the Department of Chemistry and Biochemistry in the College of Science has identified and measured the levels of about 4,000 proteins, which exhibited patterns of expression that reflect key events during early Xenopus development. For example, the appearance of organ- and tissue-specific proteins, such as those found exclusively in cardiac muscle cells, accurately reflects imminent anatomical changes taking place in the embryo. The research could lead to insight into congenital birth defects that result from the misregulation of gene expression. The research also contradicted a widely held assumption that the levels of mRNA, which encodes proteins, would be directly related to protein levels. While that was true in most cases, there were a surprisingly high number of exceptions, demonstrating that the amounts of a particular protein can be controlled by multiple mechanisms. Because development takes place in well-defined stages outside the mother, Xenopus is a favored model. Embryogenesis can be easily monitored in real time; fate maps for organ development have been determined and major regulators of these processes have been identified and characterized, providing an abundance of tissue- and organ-specific markers to track embryo formation. Additionally, embryos develop rapidly, achieving a nearly fully developed nervous system within four days. “It’s easy to manipulate the embryos to mimic certain disease states, making Xenopus extremely valuable to biologists,” Huber said. “The collaborative, ground-breaking work of Norm Dovichi, Paul Huber and their team is crucial to helping us understand the complexity of life. We are proud of this important milestone,” said Greg Crawford, dean of the College of Science at the University of Notre Dame. Dovichi and Huber co-authored the article with Liangliang Sun, Michelle Bertke, Matthew Champion and Guijie Zhu. Contact: Norm Dovichi, 574-631-2778, ndovichi@nd.edu Posted In: Research Home Experts ND in the News Subscribe About Us Related October 05, 2022 Astrophysicists find evidence for the presence of the first stars October 04, 2022 NIH awards $4 million grant to psychologists researching suicide prevention September 29, 2022 Notre Dame, Ukrainian Catholic University launch three new research grants September 27, 2022 Notre Dame, Trinity College Dublin engineers join to advance novel treatment for cystic fibrosis September 22, 2022 Climate-prepared countries are losing ground, latest ND-GAIN index shows For the Media Contact Office of Public Affairs and Communications Notre Dame News 500 Grace Hall Notre Dame, IN 46556 USA Facebook Twitter Instagram YouTube Pinterest © 2022 University of Notre Dame Search Mobile App News Events Visit Accessibility Facebook Twitter Instagram YouTube LinkedIn