key: cord-0003420-8upypiev authors: Pan, Chao-Yang; Yagi, Shigeo; Padilla, Tasha; Ng, Terry Fei Fan; Marine, Rachel L.; Nix, W. Allan; Wadford, Debra A. title: Genome Sequences of Rhinovirus Genotype C56 Detected in Three Patients with Acute Respiratory Illness, California, 2016 to 2017 date: 2018-08-23 journal: Microbiol Resour Announc DOI: 10.1128/mra.00982-18 sha: 9fa81d8d9b302402c54cefce4885df1317658c13 doc_id: 3420 cord_uid: 8upypiev We report here two genome sequences of a newly designated rhinovirus genotype, RV-C56, which were obtained from respiratory specimens of three patients with acute respiratory illness in 2016 and 2017. To our knowledge, these sequences represent the first near-complete genomes for RV-C56 strains. R hinovirus C (RV-C) is one of three rhinovirus species of the Enterovirus (EV) genus in the Picornaviridae family of nonenveloped, single-stranded, positive-sense RNA viruses. RV-C was first described in 2006 from patients with influenza-like illness (1, 2) . RV-C has been associated with more severe respiratory illness than have RV-A and RV-B (3) and is also distinct from RV-A and RV-B in that RV-C is not culturable by conventional cell culture methods (4, 5) . The California Department of Public Health Viral and Rickettsial Disease Laboratory performs diagnostic testing for many respiratory viruses, including RVs and EVs, using real-time reverse transcription-PCR (RT-PCR) assays adapted from the U.S. Centers for Disease Control and Prevention protocols (6) . Recently, we detected an increasing number of specimens that tested positive for both EV and RV by these assays. Subsequent viral protein 1 (VP1) and genomic investigations identified RV-C from some specimens, including a recent study of RV-C47 (7). We generated complete protein-coding sequences (CDSs) of three RV-C strains. Nasopharyngeal swab specimens were clarified by centrifugation, 0.4-m filtration, and nuclease treatment prior to extraction using the NucliSENS easyMAG system (bioMérieux, Durham, NC, USA). Extracted RNA was treated with DNase, followed by random RT and subsequent PCR (7, 8) . Libraries were prepared using a Nextera XT kit (Illumina, San Diego, CA, USA) and sequenced (300-cycle paired-end run) on the MiSeq platform (Illumina). The next-generation sequencing (NGS) data were analyzed by an in-house NGS pipeline performing read quality control, de novo assembly, and BLAST analysis (9) . Final consensus genomes were inspected and annotated using Geneious version 11. Average read coverage ranged from 352-to 1,863-fold for the three RV-C genomes. The genome sequences were confirmed by the Picornaviridae Study Group of the International Committee for the Taxonomy of Viruses (10) as RV-C56. In one instance, twin 1-year-old siblings with a noticeable cough but no fever yielded nearly identical RV-C56 genomes, except for two synonymous nucleotide changes in VP3. We submitted one of the two sequences to GenBank as RV-C56 strain USA/2016/CA-RGDS-1002. We identified a second RV-C56 strain, USA/2017/CA-RGDS-1003, in a 31-year-old female with low-grade fever, shortness of breath, and productive cough who had returned from overseas travel approximately 1 day prior to the onset of the illness. The genome of RV-C56 strain USA/2017/CA-RGDS-1003 shared 99.3% nucleotide identity (NI) to strain USA/2016/CA-RGDS-1002. No complete CDS of RV-C56 existed prior to this report. Both California RV-C56 strains share 96% NI to a partial capsid sequence reported from Japan in 2014 (GenBank accession number LC004772) (11) . Both California RV-C56 strains shared Ͻ85% NI and Ͻ91% amino acid identity (AI) with VP1 genes from other RV-C genotypes. Compared to the closest full genomes of other RV-C types, the RV-C56 strain USA/2016/CA-RGDS-1002 sequence is missing 26 nucleotides (nt) in the 5= end and 11 nt in the 3= end. The rhinovirus polyprotein can be divided into one structural (P1-capsid) and two nonstructural (P2 and P3) regions. The polyprotein regions of the two RV-C56 genomes reported here all share Ͻ85% NI (P1, P2, and P3) and Ͻ92% AI (P1), Ͻ93% AI (P2), and Ͻ92% AI (P3) with other RV-C genotypes. Data availability. The genome sequences of RV-C56 strains USA/2016/CA-RGDS-1002 and USA/2017/CA-RGDS-1003 have been deposited in GenBank under the accession numbers MG950178 and MG950179, respectively. The contributions of the California Department of Public Health Viral and Rickettsial Disease Laboratory were supported in part by the Epidemiology and Laboratory Capacity for Infectious Diseases Cooperative Agreement number 6 NU50CK000410 from the U.S. Centers for Disease Control and Prevention. This work was partly funded by federal appropriations to the Centers for Disease Control and Prevention through the Advanced Molecular Detection Initiative line item. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the official position of the Centers for Disease Control and Prevention. 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