key: cord-0006455-p5z5l9cn authors: Zogheib, Elie; Nyga, Remy; Cornu, Marjorie; Sendid, Boualem; Monconduit, Julien; Jounieaux, Vincent; Maizel, Julien; Segard, Christine; Chouaki, Taïeb; Dupont, Hervé title: Prospective Observational Study on the Association Between Serum Mannose-Binding Lectin Levels and Severe Outcome in Critically Ill Patients with Pandemic Influenza Type A (H1N1) Infection date: 2017-12-22 journal: Lung DOI: 10.1007/s00408-017-0067-5 sha: d2a99f3617cea44e73c60249cf5fff5dc7c701c6 doc_id: 6455 cord_uid: p5z5l9cn BACKGROUND: Mannose-binding lectin (MBL) plays an important role in the innate immune response. In addition to activating the complement, MBL can induce cytokine production and contribute to a deleterious inflammatory response with severe A(H1N1)pdm09 virus infection. Our aim was to determine if serum MBL levels correlate with the risk of mortality in intensive care units (ICU) patients with A(H1N1)pdm09 infection. METHODS: Prospective observational study was performed in ICU patients with acute respiratory distress syndrome due to influenza A(H1N1)pdm09 virus. Demographic characteristics and severity indices were recorded at ICU admission. MBL was assayed from blood drawn at influenza diagnosis within 24–48 h following the ICU admission. Outcomes were compared according to MBL levels. Results are expressed as median and interquartile range. RESULTS: Serum MBL levels were studied in 27 patients (age: 56 [IQR 29] years) with severe A(H1N1)pdm09 infection and in 70 healthy controls. Median admission SAPSII and SOFA scores were 49 [IQR 26] and 12 [IQR 5], respectively. Mortality rate after a 30-day was 37%. MBL was significantly higher in non-survivors (3741 [IQR 2336] ng/ml) vs survivors (215 [IQR 1307] ng/ml), p = 0.006, as well as control group (1814 [IQR 2250] ng/ml), p = 0.01. In contrast, MBL levels in survivors group were significantly lower than the controls group (215 [IQR 1307] ng/ml vs. 1814 [IQR 2250] ng/ml, p = 0.005). MBL cut-off > 1870 ng/ml had a sensitivity of 80% and a specificity of 88.2% for mortality [AUC = 0.82 (95% CI 0.63–0.94)]. Kaplan–Meier analysis demonstrated a strong association between MBL levels and mortality (log-rank 7.8, p = 0.005). MBL > 1870 ng/ml was independently associated with mortality (HR = 8.7, 95% CI 1.2–29.1, p = 0.007). CONCLUSIONS: This study shows that baseline MBL > 1870 ng/ml is associated with higher mortality in ICU patients with severe A(H1N1)pdm09 infection. Severe primary influenza pneumonia can progress to moderate or to severe acute respiratory distress syndrome (ARDS) [1] . Since the 1918 influenza pandemic, ''swine fluA'' and, more recently, pandemic influenza A(H1N1)pdm09, led to a significant proportion of patients with ARDS [2, 3] . Pandemic influenza A(H1N1)pdm09 is associated with a severe outcome, especially in patients with risk factors such as obesity, metabolic syndrome, pregnancy and underlying comorbidities. Although effective treatments for influenza and its complications exist, identifying early predictive factors of poor outcome in ARDS patients with influenza A(H1N1)pdm09 infection remains relevant [4] . Some studies have shown that A(H1N1)pdm09 can cause a ''cytokine storm'' with an excessive inflammatory immune response, and this could be a key factor involved in the severe outcome with this virus flu [5] [6] [7] . Mannose-binding lectin (MBL) is a glycoprotein produced by the liver and belongs to the collectin subfamily of C-type lectins. MBL is a key component of innate immunity [8] [9] [10] , as it activates complement, promotes opsonophagocytosis, and induces the production of pro-inflammatory cytokines. In inflammatory conditions, MBL can leave the blood stream via vascular leakage. In an experimental study, Ling et al. showed that MBL wild-type mice had more severe lung disease with enhanced production of pro-inflammatory cytokines and chemokines than MBL knock-out mice during A(H1N1)pdm09 virus infection, suggesting that MBL may contribute to disease severity. In their study, MBL contributed to a deleterious inflammatory response by upregulating the proinflammatory cytokines [11] . Therefore, the aim of the present study was to determine whether levels of serum MBL are associated with mortality in critically ill patients with severe A(H1N1)pdm09 viral pneumonia. This was a prospective, observational study carried out in the adult intensive care units (ICU) of Amiens University Hospital, France, between September 2009 and March 2016 in all patients in whom influenza A(H1N1)pdm09 virus infection was diagnosed during or 1-week prior to the ICU stay. Diagnosis of influenza A(H1N1)pdm09 infection was based on the presence of influenza or influenza-like illness, pneumonia, bilateral pulmonary infiltrates, and a positive result on a real-time polymerase chain reaction (RT-PCR) tests for microbiological confirmation of H1N1 influenza. Demographic and clinical data at admission and during the ICU stay were recorded from the medical files of each patient and collected in a database to evaluate variables potentially associated with in-hospital mortality. Data baselines were recorded at admission. Mechanical ventilation, extracorporeal membrane oxygenation (ECMO) requirements, and the use of vasopressor drugs were noted during ICU stay. To determine illness severity, SAPSII and SOFA scoring systems were applied to all patients within 24 h of ICU admission. This study was approved by the Institutional Review Board for Human Subjects. Patients or relatives provided their informed consent. The serum MBL of an historical control group was used to compare the levels of survivors and non-survivors. Nasopharyngeal or bronchoalveolar lavage fluid (BALF) samples were tested for the presence of A(H1N1)pdm09 virus using a RT-PCR kit according to the manufacturer's instructions (xTAG RVP Fast assay, Luminex 97 Molecular Diagnostics, Theradiag TM ). When a patient met criteria for ARDS, the closest residual blood sample taken on the same day was obtained from the central hospital laboratory in the 24-48 h following the intubation or in the acute phase of the viral infection. MBL serum concentrations were assayed by an Oligomer ELISA kit (BioPorto Ò ) according to the manufacturer's instructions. The lower limit of detection of the assay was 0.02 ng/ml. Patient serum was diluted 1:100 in the diluent buffer and incubated in the wells of ELISA plates for 1 h. After three washes in buffer, the plates were incubated for 1 h at room temperature with a secondary peroxidase-conjugated anti-MBL antibody. After another three further washes, the plates were incubated for 30 min with 200 ll of tetramethylbenzidine (TMB) at room temperature in the dark. The reaction was stopped by the addition of 100 ll of a-ready-to-use stop solution before measuring optical density at 450/620 nm. Serum MBL was quantified using a calibration curve including eight readyto-use MBL standards with a range of 0-40 ng/ml. Although there is no universally accepted cut-off concentration, MBL deficiency is defined by a serum MBL concentration B 500 ng/ml. MBL deficiency is present in 20-30% of the population, making MBL deficiency the most common hereditary immunodeficiency, as also describe by Eisen et al. [12] [13] [14] . Results are expressed as median [interquartile range (IQR)] or number (percent). Survivors were compared with nonsurvivors using the Mann-Whitney U test for continuous variables and Chi 2 test for categorical variables with Yates' correction or Fisher's exact test if necessary. A correlation was searched between MBL and inflammatory parameters using spearman test. A receiver operating characteristic (ROC) curve was constructed to assess the impact of MBL levels on mortality. The Youden index (J) was used to detect the best MBL cutoff for survival analysis. Sensitivity, specificity, positive and negative predictive values, and positive and negative likelihood ratios were calculated for this cut-off. Kaplan-Meier analysis was performed to compare the outcome of groups according to the MBL cut-off. Survival curves were compared using a log-rank test. A multivariate Cox model (backward Wald) was constructed to identify independent factors associated with mortality in this population. Adjusted Hazard Ratios (aHR) and their 95% confidence intervals [95% CI] were calculated. A p value \ 0.05 was considered to be statistically significant. Statistical analyses were performed using PASW Statistics 18 (IBM Inc., Chicago, IL, USA) and MedCalc 16.4.3 software (MedCalc Software, Ostend, Belgium) softwares. Twenty-seven patients were admitted to the ICU with severe pneumonia and with a high probability of viral infection or a previously confirmed diagnosis (median age 56 [IQR 29] years, sex ratio 17 men/10 women). All patients had a confirmed diagnosis of influenza A(H1N1)pdm09 virus infection. They had signs of viral infection, including fever, myalgia, and cough, and were treated with oseltamivir (150 mg/day) for 7 days. All patients with pulmonary symptoms received empirical antimicrobial therapy with ceftriaxone and rovamycin or levofloxacin on admission and this was subsequently adapted to any documented bacterial infection if positive. (Fig. 1) . The ROC analysis for mortality is shown in Fig. 2 Pandemic influenza A(H1N1)pdm09 virus causes significant morbidity and mortality in humans. Some studies have shown that influenza A(H1N1)pdm09 infection can induce dysregulation of the immune system, such as cytokine storm and excessive inflammatory infiltrates, leading to lung injury [11] . Among these factors, excessive cytokine production is considered to be the key contributor in severe response during influenza A(H1N1)pdm09 virus infection [5] . MBL contributes to a deleterious inflammatory response by upregulating the pro-inflammatory response [11] . In our study, we determine the serum levels of MBL in critically ill patients with severe A(H1N1)pdm09 infection and analyzed the correlation between plasmatic MBL levels and the risk of mortality. In our study, a serum MBL level [ 1870 ng/ml was independently associated with mortality in ICU patients with influenza A(H1N1)pdm09 virus infection and therefore could predict an increased mortality at 30 days. MBL belongs to the collectin family and functions as a pattern-recognition molecule recognizing a wide range of pathogens. It plays an important role in innate immunity [10] . MBL may represent an important barrier to the establishment of influenza virus infection in epithelial cells. The antiviral activity of MBL against influenza viruses remains controversial. Previous in vitro studies have suggested that MBL exhibits anti-influenza virus function by direct neutralization after binding to mannoserich glycans on haemagglutinin (HA) and neuraminidase (NA) glycoproteins [15] [16] [17] . However, this needs to be balanced as the antiviral function of MBL may vary among different strains of influenza viruses, depending on the number of potential glycosylation sites on the viral haemagglutinin globular domain. MBL has been shown to inhibit influenza A seasonal H3N2 and seasonal H1N1 viruses through direct neutralization, indirect opsonization, and complement activation, but limited antiviral effect against H7N9 virus [18] . In contrast, MBL can bind to influenza A(H1N1)pdm09 virus but fails to inhibit its infection in human lung epithelial cell line. Ling et al. have demonstrated in an experimental study that upon A(H1N1)pdm09 infection, MBL is internalized with the virus into the cell, where it may associate with TLR3 to further amplify the NF-jB signaling and augment the cytokine production in the human lung epithelial cells (supplementary data). Their findings advocate the adverse immunomodulating role of MBL during A(H1N1)pdm09 BMI body mass index, SAPS II simplified acute physiology score, SOFA sequential organ failure assessment, COPD chronic obstructive pulmonary disease, ARDS acute respiratory distress syndrome, IA Invasive aspergillosis, MBL mannose-binding lectin, ECMO extra corporeal membrane oxygenation, WBC white blood cells, PMN polymorphonuclears infection by upregulating pro-inflammatory response [11] or may contribute to the pathogenesis of inflammatoryinduced vascular damage and organ failure, as observed in patients undergoing solid organ transplantation, leading to a harmful effect of an excessive MBL expression [19] . In our study, serum MBL concentrations observed were very variable from one patient to another, but clearly lower levels in the survivors group than the non-survivors group comparing to the control group (Fig. 1) . This heterogeneous distribution in the control group can be explained by individual variations in serum MBL levels that may reflect gene polymorphism ranging from (i) homozygotes with a mean MBL concentrations of 2660 ng/ ml (range 40-6790 ng/ml), (ii) heterozygotes with mean MBL concentrations of 830 ng/ml (range 0-4460 ng/ml, and (iii) compound heterozygotes and mutant homozygotes with mean MBL concentrations of 40 ng/ml (range 0-300 ng/ml). Genotype is one of the determinant factors of serum MBL concentrations. However, MBL levels vary significantly among individuals with the same genotype, and the serum MBL concentration may more accurately reflects downstream effector function than genotype. MBL levels do not vary with respect to age, sex, physical exercise, season, time of day, or liver function [16] [20] and are stable during storage at -80°C through a number of freeze-thaw cycles. Despite these differences in MBL concentrations due to genetic variations, the decrease in serum MBL levels due to MBL consumption during pathogen binding is compensated by an increase in MBL gene transcription during acute infection [12, 13] . Usually, MBL acts as a first line of innate immune response against microbial infections, and it may regulate pro-inflammatory cytokine and chemokine release from phagocytes in response to bacterial and fungal stimulations [21] [22] [23] . Many studies have shown that low serum MBL levels are associated with a susceptibility to bacterial, fungal and viral, and leads to a worse outcome [14, 24] . On the other side, sporadic reports have not found a clear [28] . Other glycoproteins are involved in the regulation of innate immunity and the antiviral responses such as SP-D. The high serum level of SP-D was described as a predictor of poorer outcomes in ICU patients with ARDS associated with influenza A(H1N1)pdm09 virus infection [29] . In our study, the serum MBL levels [ 1870 ng/ml were independently associated with mortality in critically ill patients with severe influenza A(H1N1)pdm09 virus infection. Moreover, the serum MBL levels are not affected by any respiratory chronic disease in contrast to SP-D which could be a prognostic biomarker of clinical exacerbations and COPD progression as presented by Sin et al. [30] . Our study has several limitations. The sample size was restricted by the study's focus on critically ill patients with virologically confirmed influenza A(H1N1)pdm09 virus. Therefore, we should be cautions with our interpretation of data. Another limitation is the absence of a control group of patients with ARDS associated with respiratory viruses. It would be of interest to determine whether MBL variations are related to others respiratory virus or not. As for all biomarkers, the range of MBL concentrations is very wide and it is difficult to know if a patient is in the group with a better prognosis or not. Larger studies should better define this range. Serum MBL levels [ 1870 ng/ml were independently associated with mortality in critically ill patients with severe influenza A(H1N1)pdm09 virus infection. This protein could be a potential early biomarker of prognosis. Future studies are required to confirm these results. 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The cooperation of the medical and paramedical staff of intensive care units of the University Hospital, Amiens, France, is gratefully acknowledged. Authors' contributions EZ, RN, TC, and HD contributed to the conception and design of the study. EZ, RN, and TC performed the acquisition of the data. HD performed the statistical analysis. EZ, RN, TC, JM, VJ, JM, CS, SB, and HD contributed to the analysis and interpretation of data, wrote and approved the final manuscript. EZ, RN, TC, JM, VJ, JM, CS, SB, and HD gave agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.Funding This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors. Conflict of interest All the authors declare that they have no conflict of interest.Ethical approval This study was approved by the Institutional Review Board for Human Subjects.Informed consent Patients or relatives provided their informed consent.