key: cord-0009577-26yc50h3 authors: Dunn, Ewan F.; Connor, John H. title: HijAkt: The PI3K/Akt Pathway in Virus Replication and Pathogenesis date: 2012-02-13 journal: Prog Mol Biol Transl Sci DOI: 10.1016/b978-0-12-396456-4.00002-x sha: e2a3c218897f82841787e8caa3c96da8e560c9b7 doc_id: 9577 cord_uid: 26yc50h3 As obligate parasites of cellular processes, viruses must take over cellular macromolecular machinery. It is also becoming clear that viruses routinely control intracellular signaling pathways through the direct or indirect control of kinases and phosphatases. This control of cellular phosphoproteins is important to promote a variety of viral processes, from control of entry to nuclear function to the stimulation of viral protein synthesis. This review focuses on the takeover of the cellular phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathway by a variety of retroviruses, DNA viruses, and RNA viruses, highlighting the functions ascribed to virus activation of PI3K and Akt activity. This review also describes the role that the PI3K/Akt pathway plays in the host response, noting that it that can trigger anti- as well as proviral functions. Viral disease is one of the most significant human health concerns in modern medicine, manifesting as a broad range of acute infections, chronic diseases, and virus-associated malignancies. Viruses such as human immunodeficiency virus (HIV), hepatitis C, influenza, rotavirus, chikungunya, and dengue affect millions of people each year worldwide. The emergence of novel strains of H1N1 flu 1 and previously unrecognized pathogens such as severe acute respiratory syndrome (SARS) coronavirus 2 and SFTS bunyavirus 3, 4 has highlighted the threats of viral emergence and pandemic with which humanity regularly contends. This, along with the emergence of antiviral resistance among viruses such as influenza, highlights the need to better understand virus replication and the virus-host interaction to identify critical restriction points that can be targets for antiviral drug development and vaccine generation. One controlling aspect of viral pathogenesis and replication is their dependence on the cells that they infect. Unlike other microbial pathogens such as bacteria and fungi which are capable of autonomous growth, viruses are completely dependent on cellular processes for replication. It has long been recognized that viruses are dependent upon the use of cellular machinery for protein synthesis: nucleotide and protein building blocks, ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) polymerases, and membranes. More recently, it has become clear that viruses also rely upon intracellular communication. 5, 6 Particularly, viruses appear to be adept at capturing protein phosphorylation cascades for their own use. Most, if not all, viruses require protein phosphorylation in order to replicate. This would initially suggest that viruses would gain a replication edge by generally promoting protein phosphorylation. However, simply promoting all cellular protein phosphorylation is not likely to be advantageous to viruses, as the activity of some host kinases (and the resultant phosphorylation events) are known to antagonize viral replication. Thus, a nonspecific increase in phosphorylation would trigger an antiviral response. Viruses have a need for specificity in activating host kinases and phosphatases. Simply stated, viruses need to activate signaling pathways that promote their replication [7] [8] [9] while blocking or avoiding the activation of antiviral signaling pathways. 10 Controlling kinase activity can promote protein translation, 11 increase metabolic activity, drive cell division, and inhibit cellular apoptosis. All of these are advantageous for virus replication. In addition, many viruses produce virus proteins that require direct phosphorylation in order to properly function but make no kinase that will carry out this phosphorylation. 9, 10 This dictates that they must recruit kinase activity from their host. It is therefore not surprising that viruses have developed mechanisms to control cellular signaling through regulating kinase and phosphatase signaling cascades. What is surprising is the commonality of host pathways that are targeted. In theory, any number of kinases might be important for the replication of any particular virus, but this possibility of each virus using different kinases is not actually observed. Rather, as is emphasized below, one signaling pathway has emerged as a common player in the replication of many different viruses-the phosphatidylinositol-3-kinase (PI3K)/Akt phosphorylation cascade. The repeated identification of the PI3K/Akt pathway as a central player in virus replication has underscored the importance of this pathway as a central regulator of cell health and metabolism. As a central regulator of multiple cellar processes that control translation, metabolism, and cell death, active PI3K/Akt signaling can fulfill many viral ''needs.'' This is not to suggest that this signaling pathway only provides positive feedback for the virus. Studies of virus interaction with this pathway have also shown that the pathway is not simply a ''proviral'' kinase when activated, but that it also acts as part of the host response to viral infection. This suggests that PI3K/Akt signaling represents a two-faced player in interactions between the virus and cell, able to both promote viral replication and be an active part of the immune response that will eventually quash virus replication. This review will describe the different approaches that viruses have taken to dominate the PI3K/Akt signaling pathway. The viruses chosen in this review highlight the varied manner in which this pathway is manipulated and turned to the advantage of the invader, while also pointing out how the pathway also plays a role in host defense. The PI3K/Akt signal transduction cascade is a classical phosphorylation cascade that utilizes tyrosine, lipid, and serine-threonine phosphorylation to transduce external signals to internal responses (see Fig. 1 for a schematic diagram). Normally, signaling through this pathway is initiated by the stimulation of a receptor tyrosine kinase (RTK) by a cytokine or a growth factor at the cell surface. Activation of the RTK recruits and activates PI3K. PI3K is a heterodimer consisting of a p110 catalytic and a p85 regulatory subunit. 12 PI3K is responsible for converting phosphatidylinositol 4,5-bisphosphate (PIP2) to 3,4,5-triphosphorylated phosphoinositide (PIP3). PIP3-rich membrane domains serve as docking sites for proteins that contain a pleckstrin homology domain such as Akt. The recruitment of inactive Akt protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of Akt. 13 Akt's activating kinase, phosphoinositide-dependent protein kinase (PDK1) 14 , is also recruited to PIP3 microdomains. PDK1 phosphorylates Akt on threonine 308 (Thr308) of the exposed activation loop, activating Akt and leading to a second phosphorylation of Akt at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. 13, [15] [16] [17] Activated Akt can control the signaling of several key pathways supporting different types of cellular functions (Fig. 1) . The PI3/Akt signaling pathway promotes cell growth, cell survival, and tumorigenesis through the phosphorylation and inactivation of cell cycle kinase inhibitors such as p21 and the 1 . Overview of the PI3K/Akt pathway. The diagram illustrates the two subunits of PI3K (p85 and p110) bound to an activated receptor tyrosine kinase (classical examples are the epidermal growth factor and the insulin receptor). Active PI3K catalyzes the addition of a single phosphate to the 3 position of PIP4,5-bisphosphate to make PIP3. This triphosphorylated inositol serves as an affinity ligand for the pleckstrin homology domains of the Akt kinase PDK1 and Akt, with the binding of PIP3 by the Akt PH domain serving to uncover the activation domain of Akt. Phosphorylation by PDK1 occurs at position T308, and a second activating phosphorylation occurs at S473. Active Akt will phosphorylate various downstream protein targets that control cell growth (AMPK, p21, and GSK3 are illustrated) and translational control (mTOR and its downstream effectors p70S6K and 4E-BP1 are illustrated) and act to suppress apoptosis (inhibitory phosphorylation of BAD and FOXO1 are illustrated). inactivation of transcription factors that can inhibit cell cycle progression and promote apoptosis such as the forkhead family of proteins (e.g., FOXO1 18 ). Akt also can block the actions of metabolically repressive kinases such as AMPK 19 and so can drive the up regulation of metabolic activity in the cell. 20 Akt also promotes cellular translation through GSK3 phosphorylation and the activation of mammalian target of rapamycin complex 1 (mTORC1), a kinase complex that activates ribosomal S6 kinase and inhibits the translational repressor eIF4E-BP1 (4E-BP1). 21 Akt activation of mTORC1 is indirect. Akt phosphorylates and represses the hamartin and tuberin complex (TSC1 and TSC2 22 ). Inhibition of TSC1/TSC2 allows Rheb-mediated activation of mTORC1 complex kinase activity. MTORC1 phosphorylates and activates the ribosomal protein S6 kinase (p70S6K) and inactivates the translation suppressor 4EBP1, leading to increased translation. Akt activity antagonizes apoptotic signaling. This is accomplished through direct actions such as inhibiting pro-apoptotic factors (e.g., phosphorylation and inhibition of BAD, 23 a pro-apoptotic member of the BCL-2 family) and through more indirect actions, such as the activation of the transcription factor nuclear factor kappa B (NF-kB) 24 and, as mentioned above, the inactivation of FOXO1. With actions that influence multiple phosphoprotein cascades involved in different core aspects of cell function, PI3K/Akt signaling pathway is a prime candidate for a ''hub'' kinase whose activation can have a diverse set of consequences, all of which are centered on promoting cell survival and increasing the metabolic capacity of the cell. With the central nature of the PI3K/Akt pathway in controlling cell functions, it stands to reason that the control of such a signaling ''hub'' would greatly benefit an invading virus that is dependent upon cellular functions. Extensive research supports this hypothesis, finding viral domination of this pathway in various contexts. 5, 25, 26 To illustrate the different ways in which viruses attack and control the pathway, this review will explain different examples of how viruses co-opt the host signaling apparatus through the PI3K/Akt pathway for selfish gain. The review will also highlight how viral activation of this pathway can contribute to the host's response to viral infection. This review will classify virus interactions with the PI3K/Akt pathway based on the aspect(s) of PI3K/ Akt signaling from which each virus benefits. This approach is complementary to other reviews which have taken the approach of classifying the interaction based on virus families. 5, 26 The first examples presented here highlight viruses that are suggested to have ''simple'' interaction paradigms. For the purpose of this review, a ''simple'' interaction is one where the virus or virus family appears to activate the PI3K/ Akt pathway to benefit from just one aspect of its cellular control, such as the promotion of cell cycle progression, or the inhibition of apoptosis. Following this, examples of viruses that benefit from several of the outcomes of PI3K/Akt signaling will be discussed. A. Simple Paradigms of Viruses That Interact with PI3K/Akt 1. VIRAL PROTEINS THAT USE MOLECULAR MIMICRY TO ACTIVATE THE PI3K/AKT SIGNALING AXIS Several viral proteins are known to directly activate the PI3K/Akt signaling axis through molecular mimicry or replacement (Fig. 2) . These viral proteins helped in elucidating the initial understanding of the PI3K/Akt signaling cascade. The paradigm example v-akt was originally described as a retroviral oncogene encoded by the AKT8 mouse transforming retrovirus. 27 The kinase was described as a fusion protein with the viral gag (matrix protein). It was quickly recognized that the protein had a cellular homolog, 28 which was named c-akt. This protein was later determined to be the same as the cellular kinases that had been characterized under the names of PKB 29 and RAC-PK. 30 Though the role of v-akt in the life cycle of the AKT8 virus was never established, v-akt is capable of transforming cells, suggesting that the virus utilized the v-akt protein to drive cell cycle progression. The finding that a cellular kinase had been essentially directly incorporated into the viral genome as a fusion protein with the viral gag protein was the first demonstration of the importance of this kinase for viral replication. The control of the PI3K/Akt signaling pathway by molecular replacement extends to several other viruses, notably avian sarcoma viruses. The AV16 sarcoma virus encodes for a PI3K homolog, named vP3K, 31 which encodes a homolog of the cellular catalytic subunit of PI3K (p110). Like v-Akt, vP3K acts to provide constitutively active PI4,5-bisphosphate kinase activity without the need for the p85 regulatory subunit, generating high levels of PIP3. This serves to activate Akt, 32 which phosphorylates and inactivates the FOXO1 transcription repressor 33 and promotes cellular translation through the phosphorylation of 4E-BP1. 34 These actions remove a cell-cycle brake, allowing proliferative signals to dominate. A slightly different approach to activation of the PI3K/Akt pathway activation is taken by the CT10 and AV1 viruses. These viruses each encode a homolog to the cellular Crk protein. [35] [36] [37] vCrk activates the PI3K/Akt signaling pathway through the recruitment of PI3K to the focal adhesion kinase. 38 This activity leads to the transformation of vCrk-infected cells 39 presumably by mechanisms similar to those described for vP3K (activation of cellular translation and inactivation of transcription factors such as FOXO1). The diagram illustrates steps at which viral proteins either replace or actively modulate Akt signaling. Shown are the vPI3K (p110 subunit) produced by the avian retrovirus A16, and the vAkt protein produced by the mammalian AKT8 retrovirus which directly activates Akt signaling. Also shown is vCrk, which stimulates Akt signaling in a focal adhesion kinase (FAK)-dependent manner. Illustrated on the right-hand side of the figure is the SV40 small t (smt) protein. The smt protein binds the PP2A/C heterodimer and inactivates Akt dephosphorylation, providing an indirect but potent activation of Akt kinase activity. The actions of all these proteins can drive cell proliferation in a manner that is dependent on Aktphosphorylated proteins. In all of these examples, a consistent theme is evident-retroviruses have repeatedly captured host proteins that can activate the PI3K/Akt pathway. It is thought that the cell proliferation induced by these proteins is critical for the replication of these viruses, as the breakdown of the nuclear envelope during the cell cycle gives these retroviruses access to cellular DNA and thereby allows the virus to integrate within this DNA. Further, proliferating cells are more likely to activate retroviral gene expression to stimulate virus production. However, this may not be the only role for the PI3K/Akt pathway in the retrovirus life cycle, and more recent research into viruses, such as HIV, suggests that the virus may benefit from inducing Akt activity to prevent premature apoptosis. 40 Control of Phosphatase Activity Viruses have also adopted approaches of inducing PI3K/Akt pathway signaling by attacking phosphatase activity. As is true in most kinase cascades, the activity of Akt signaling can be decreased through phosphatase-mediated dephosphorylation of Akt activation domains. Conversely, kinase signaling can be potentiated by decreasing phosphatase activity. For PI3K/Akt signaling, the cellular phosphatase PP2A is at least partially responsible for the dephosphorylation and inactivation of Akt. 41, 42 By decreasing the activity of PP2A, more Akt will remain in the active state. This approach to activating Akt signaling has been adopted by the genus Polyomaviridae. Simian virus 40 (SV40) is the prototype example of a polyomavirus that stimulates Akt activation by inhibiting the dephosphorylation of Akt. 43 To do this, the SV40 small t antigen of SV40 (SV40ST) binds the cellular phosphatase PP2A that is responsible for a large fraction of Akt phosphatase activity. 44 PP2A is a heterotrimeric protein comprising a scaffolding subunit (A), a catalytic subunit (C), and a regulatory subunit (B). There are many variants of the B subunit, and each is believed to be responsible for directing the phosphatase catalytic subunit directly to a single substrate (such as Akt) or set of substrates. SV40ST displaces the B subunit from the A and C PP2A subunits, inhibiting much of the phosphatase activity which would normally dampen or reverse Akt and mTOR activation (see Fig. 2 ). The resulting potentiation of Akt signaling helps drive cell proliferation, moving the cell into S phase, where virus DNA synthesis is initiated. This modulation of PP2A to alter PI3K/Akt signaling is utilized by other viruses in the polyomavirus genus as well, including BK virus, 45 mouse polyoma virus, 46 and JC virus. 47 In addition, polyomaviruses can indirectly stimulate the PI3K/Akt pathway through additional mechanisms, [48] [49] [50] indicating that these viruses attack the PI3K/Akt pathway at multiple points to ensure pathway activation. A similar mechanism of activating Akt signaling (inhibition of PP2A activity that increases Akt activation) has been proposed for the E7 oncoprotein of papillomavirus HPV. 51 The HPV E7 protein is critical for the maintenance of cellular DNA synthesis in HPV-infected cells, 52 which is a necessity, as HPV requires the host cell DNA synthesis machinery for its replication. 53 Thus, activation of Akt to remove brakes on cell DNA synthesis (i.e., FOXO) is of clear value to virus replication. This action is also important in the cases where the E7 gene is incorporated into host DNA (not part of the normal virus life cycle). Under these circumstances, Akt signaling may promote malignant transformation, 51 thereby underscoring a potential link between viral control of cellular phosphorylation for its own means and the induction of cancer. In the examples presented above, virus takeover of the Akt signaling pathway was associated with the induction of cellular proliferation. In many examples, though, viruses that activate the PI3K/Akt pathway do not benefit from the promotion of cellular proliferation or cellular transformation. For many viruses, the PI3K/Akt pathway can provide the short-term benefit of keeping the infected cell alive through the suppression of apoptotic signals. The critical nature of PI3K/Akt-mediated suppression of apoptosis is best illustrated by viruses that cause acute infections, such as the RNA viruses. For most RNA viruses, their life cycle is carried out in the cytoplasm of their host cell. These viruses therefore have minimal requirements for nuclear components or cell cycle progression, and in many cases block nuclear functions and cell-cycle progression. [54] [55] [56] Thus, PI3K/Akt-mediated promotion of cell division or alteration of transcription does not play an essential role in virus replication. Instead, phosphorylation of cytoplasmic proteins in order to delay apoptosis keeps the host cell alive until viral progeny can be produced. The trigger for PI3K/Akt activation in infection with paramyxoviruses often occurs early in infection. As an example, respiratory syncytial virus (RSV), a significant pediatric pathogen, activates PI3K/Akt signaling at the level of PI3K activation. 57 Pathway stimulation appears to involve the activation of sphingosine kinase, which, in turn, leads to the phosphorylation and activation of Akt through a mechanism that may involve the extracellular signaling of sphingosine 1 phosphate. 58 Akt activity is critical for delaying cellular apoptosis during RSV infection. 57 Similarly, the genetically related Sendai virus, human parainfluenza virus 5 (HPIV5), and HPIV3 have also been shown 59 or suggested 59, 60 to activate PI3K and Akt early in infection, an action associated with delaying apoptosis and potentially activating the viral polymerase. For all of these viruses, signaling through the Akt pathway allows the completion of the viral replication cycle. Inhibition of PI3K or Akt signaling leads to faster apoptosis 59 and, in some reports, reduced virus growth. 60 In the case of Sendai virus, Akt activation may also play a role in the ability of this virus to establish a persistent infection in cells in tissue culture. 59 Viruses in the picornavirus family are also reported to activate PI3K/Akt signaling. For both poliovirus and the common-cold-causing rhinovirus, viral attachment and entry serve as the trigger for PI3K/Akt activity. 61 For poliovirus, the exact trigger is unknown; for rhinovirus, binding of the virus to its receptor recruits the tyrosine kinase Syk, which interacts directly with the p85 subunit of PI3K and activates PI3K/Akt signaling. 62, 63 It is likely that the activation of Akt is important for avoiding cell death in the case of both viruses. An important role for Akt in forestalling apoptosis has been shown in the case of poliovirus, where the Akt phosphorylation of Ask1 limits the activity of its downstream effector Jnk1 and thereby decreases apoptotic signals. Cardioviruses also appear to manipulate Akt activity to limit apoptosis in infected cells. For both encephalomyocarditis virus (EMCV) and coxsackievirus, viral infection is associated with the stimulation of PI3K/Akt activity. 64 Unlike poliovirus and rhinovirus infection, the activation of PI3K does not appear to be entry dependent. 64 Activation is important for suppression of apoptosis and promotion of virus replication. For coxsackievirus, Akt activation results in the stimulation of NF-kB activity, which promotes cell survival. 65 6. ACTIVATION OF AKT SIGNALING BY REOVIRUSES PI3K/Akt activation to promote the survival of infected cells is also seen during infection of cells with double-stranded RNA viruses of the reovirus family. As an example, rotavirus stimulates PI3K/Akt activity within the first 2 h of infection. 66 Through the use of both mutant virus and viral protein overexpression approaches, it has been shown that Akt activation is carried out by the rotavirus nonstructural protein 1 (NSP1). 67 The activation of Akt by NSP1 can occur even in the absence of other viral components and involves a direct interaction between NSP1 and PI3K (see Fig. 3 ), suggesting that the protein alters kinase activity. The mechanism of this targeting is currently unknown. Activation of Akt has also been suggested for the avian reovirus A1133. 68 The activation of Akt in rotavirus-infected cells suppresses apoptosis to allow the completion of the virus life cycle. 69 Akt signaling in infected cells is also required for the expression of integrin proteins 66 which can strongly promote both survival signaling and virus attachment, suggesting that Akt activation may send survival signals and promote viral attachment in infected cells. Like many viruses, the flaviviruses dengue virus and Japanese encephalitis virus 70 stimulate PI3K/Akt activation within the first few hours of infection. For these viruses, the mechanism of activation is unknown, but activation appears to be transient 70 , a viral ortholog of the Akt kinase. Illustrated on the right-hand side of the diagram is action of the hepatitis C protein NS5A to bind FKBP38, relieving an inhibitory regulation to activate mTOR. Also illustrated is action of UL38, which inhibits TSC2, also relieving an inhibitory regulation to activate mTOR. either PI3K or Akt but has been shown to promote cell survival through the activation of the downstream effector, mTOR kinase. 71 This activation is facilitated by the viral nonstructural protein NS5A, which has the capacity to bind directly to FKBP38, a negative regulator of mTOR 72 (see Fig. 3 ). The association of FKBP38 with mTORC1 is blocked by NS5A binding, thereby stimulating mTORC1 activity, promoting translation, and limiting apoptosis. This mechanism of targeting mTORC1 by relieving an inhibitor action is seen in the actions of a cytomegalovirus (CMV) virus protein, UL38 (see below). As described in the introduction, the PI3K/Akt pathway acts as a signaling ''hub'' triggering a branching phosphorylation cascade that alters multiple cellular processes. The viruses described above appear to benefit primarily from one aspect of ''hub'' activation or one subset of the phosphoproteins. This is not true of all viruses. Some viruses benefit from more than one signaling arm of the PI3K/Akt pathway. Three examples of this more complex virus/host interaction illustrate this well: the domination of signaling by herpes viruses, poxviruses, and the influenza virus. The herpes virus family consists of several viruses that have each taken differing approaches to achieve the same result: preservation of a favorable environment for both viral transcription and viral translation. The life cycle of these viruses is particularly pertinent to their interaction with the PI3K/Akt pathway, as herpes viruses have both a lytic phase, where they actively replicate in cells, and a latent phase, where they are maintained as episomes in the host genome. This dual life cycle means the virus requires different cell environments at different times. During latent infection, the virus requires little from the host, while during the lytic phase, many host factors and a high metabolic throughput are desirable. One example of how herpes viruses interact with PI3K/Akt signaling is the Epstein-Barr virus (EBV). EBV is associated with Burkitt lymphoma/leukemia and other lymphoproliferative disorders. EBV establishes a latent stage in which most viral proteins are not produced and replication is dormant. In this phase, virus-infected cells produce a latency protein LMP2A. LMP2A promotes Akt phosphorylation, provides a general antiapoptotic signal, and also promotes c-MYC translation through translational upregulation caused by mTOR activation. 73 The development of EBV-driven Burkitt lymphoma/leukemia is dependent upon the constitutive expression of c-MYC, which modulates the expression of target genes that encode many cellular processes including cell growth, division, and apoptosis. LMP2A protects cells that express c-MYC from apoptosis through the upregulation of antiapoptotic genes. 74 LMP1, another latency-associated protein produced by the EBV genome, also activates the Akt pathway. 75 For LMP1, the activation of Akt activity is associated with a nuclear function-the inhibition of DNA damage repairand not protein synthesis promotion. This suggests the LMP1 and LMP2 proteins of EBV target different pools of PI3K and Akt to control the function of this signaling pathway in different cellular compartments. The activity of Akt is also targeted in the lytic phase of EBV replication. Expression of BRLF1, a protein that induces lytic replication of EBV, results in the activation of Akt in overexpression studies, suggesting that pathway PI3K activity is an important signal for the emergence of the virus from latency. 76 Consistent with this, inhibition of PI3K signaling blocks EBV reactivation from a latent state. This dual use of the PI3K/Akt signaling for both latent and lytic replication reveals an interesting paradigm for cellular signaling in virus infection. Akt activity appears to be a basic requirement for the survival of EBV-infected cells, but cannot in itself be a trigger for reactivation. Akt activity is much more likely to be a basal maintenance factor. Further research will undoubtedly uncover true triggers that cooperate with the PI3K/Akt pathway to drive reactivation. In the interim, the apparent requirement for PI3K/Akt signaling to maintain a latent state and help drive reactivation has identified an Achilles heel of EBV-infected cells. Targeting EBV-infected cells for death by adding inhibitors of this pathway are showing initial promise as a way to inhibit EBV þtumor growth. 77 3. HERPES VIRUS ACTIVATION OF PI3K/AKT SIGNALING Unlike EBV, the activation of the PI3K/Akt phosphorylation cascade in herpes simplex virus 1 infection is transient. 78 The activation has been proposed to be due to the actions of the viral VP11/12 protein, 79 though virus binding to the cell surface has also been proposed as the activating trigger. 80 Despite the transient nature of Akt activation, 78 downstream effectors of Akt such as 4E-BP1 remain phosphorylated throughout virus infection. 81 The preservation of the phosphorylated state appears to be the responsibility of the viral kinase UL3. UL3 is capable of phosphorylating multiple Akt substrates including TSC2, GSK3, and the transcription factor FOXO. UL3 appears to stimulate the assembly of translation initiation factor complexes that drive the translation of viral mRNAs, though additional actions seem likely 81 as the phosphorylation of multiple Akt substrates suggest UL3's kinase activity impacts multiple signaling pathways (Fig. 3) . This host kinase mimicry is functionally analogous to that seen for the retroviruses described above, where a virus acquires or evolves a kinase that phosphorylates the same substrates as Akt. The HSV example is distinct in that UL3 is not homologous to Akt at a protein sequence level. Unlike EBV and HSV, CMV is a herpes virus family member that has not been shown to directly or indirectly activate PI3K or Akt. Instead, CMV targets the downstream effector mTOR through a viral protein/host protein interaction strategy. CMV encodes a protein, named UL38, 82 that interacts directly with the tuberous sclerosis protein 2 (TSC2), a protein that is involved in suppressing mTOR activity. Binding of UL38 acts to ''silence'' the TSC2 inhibition of mTOR activity (Fig. 3) , resulting in an increase in mTOR substrate phosphorylation. 82, 83 This action phenocopies Akt activation, as Akt will directly phosphorylate and inhibit TSC2. By producing UL38, CMV replaces this function of Akt activation. Further, the UL38 inactivation of TSC2 also blocks the ability of the metabolic sensor AMPK to modulate mTOR activity by activating TSC2. In CMV-infected cells, expression of UL38 acts to preserve the mTOR arm of the PI3K/Akt pathway, an action that promotes viral translation and replication. Similar to the multifactor targeting of the PI3K/Akt pathway seen in EBVinfected cells, it has also been reported that CMV-infected cells show a change in the substrate specificities of the mTOR complexes mTORC1 and mTORC2. 84 These changes are not associated with the UL38 protein but may be important for a change in substrate specificities that accompany a localization of mTORC1 to the replication centers of CMV in infected cells. 85 These findings suggest that there are mechanisms of mTOR control in addition to UL38-mediated activation of mTOR. Poxviruses are some of the most genetically complex viruses known, expressing more than 200 genes from a DNA genome, including viral homologs of many cellular proteins. While a homolog of PI3K or Akt is not present, signaling through the PI3K/Akt pathway is critical for virus replication. For vaccinia, cowpox, 86 and myxoma viruses, 87 PI3K/Akt activity is rapidly induced following productive infection. This activation appears to play at least three distinct roles during poxvirus infection. One is the suppression of apoptosis, which has been suggested to be important to allow proper viral replication. 86 The second is the promotion of assembly of the eIF4F translation initiation factor complex through mTOR-mediated inhibition of 4E-BP1 function. This promotes virus mRNA translation. 88, 89 PI3K/Akt activity appears to be especially critical for allowing the transcription of mRNAs that encode for proteins involved in the assembly and budding of poxviruses. 86 The third role for PI3K/Akt is at a late stage of viral infection, where it is important for the proper progression of morphogenesis. 89, 90 Addition of PI3K inhibitors during infection or deletion of the p85 alpha and beta subunits of PI3K altered multiple aspects of vaccinia replication. 90 Of note were a decrease in late gene expression and the progression of the virus particle from an immature form to the intracellular mature virus. At least one poxvirus, the rabbitpox virus (myxoma), produces a protein dedicated to harnessing and activating Akt. This protein, named M-T5, is an ankyrin-repeat protein. It was recently shown that this protein binds directly to Akt (see Fig. 3 ) and promotes its activation and signaling in a manner that is analogous to the cellular protein PikeA. 87,91 M-T5 not only activates Akt but also forces relocalization of nuclear Akt to the cytoplasm. 92 Removal of M-T5 from the myxomavirus genome results in a markedly attenuated virus that is incapable of causing lethal disease in rabbits. 93 This highlights the importance of Akt activity for poxvirus pathogenesis. How Akt activation supports the pathogenesis of virus replication in an animal model is not fully resolved. Also unknown at this point is whether other poxviruses such as smallpox or monkeypox have proteins analogous to M-T5 that function to activate PI3K/Akt signaling. As one of the most significant endemic pathogens confronting humanity, influenza virus represents a virus that has repeatedly shown its ability to infect and adapt. As a virus that produces fewer than a dozen proteins, it is not surprising that host factors aid in influenza replication. What is striking about influenza is the extent to which the virus appears to utilize Akt-related signaling throughout the viral life cycle. Binding of influenza virus to the cell surface leads to the activation of Akt, potentially through clustering of RTKs such as the epidermal growth factor receptor (EGFR). 94 While a role for PI3K in flu virus entry seems clear, other signaling pathways (e.g., the ras signaling pathway) also appear to cooperate with the PI3K/Akt pathway to promote virus entry. 95 The importance of PI3K/Akt signaling for influenza continues after infection. Following virus entry, influenza A virus directly activates Akt signaling 96, 97 through the binding of the viral NSP1 to the P85 subunit of PI3K 96, 98 (Fig. 3) . NS1 binds to p85 at a coiled-coil region of p85, 99,100 relieving contacts between p85 and p110 subunit of PI3K that normally inhibit activity. The resulting activation of PI3K dramatically stimulates Akt activity in influenza-infected cells. For some strains of flu, it has been shown that NS1 will also bind the cellular adaptor Crk, an action which further potentiates PI3K/Akt signaling. 101 Functionally, this activation of Akt signaling has been proposed to lead to the inhibition of apoptosis and to allow the preservation of cell integrity during the viral replication cycle. 102, 103 However, this may not be universally true. Viruses lacking NS1 function are stronger inducers of apoptosis during infection, but stimulation of Akt activity in viruses that lack the NS1 protein does not inhibit apoptosis. This suggests the true role for Akt activation during the replication of influenza is yet to be defined. The PI3K/Akt pathway is also a signaling pathway that is utilized in the cellular defense against invading pathogens. As described above, phosphorylation signals are the backbone of the antiviral response. Akt signaling is increasingly recognized to play a role in this response. 104 This is particularly true for the host response to interferon, where PI3K and Akt signaling potentiates the translation of interferon-stimulated genes through the actions of mTOR and p70S6K which stimulate interferon-responsive gene translation. 105, 106 Akt also plays a role in stimulating the production of inflammatory cytokines by contributing to the activation of the NF-kB transcription factor. 57 There are many examples of virus-mediated activation of Akt signaling leading to the activation of host innate immune genes such as inflammatory cytokines. Akt activation results in the increased production of proinflammatory cytokines IL-6 and IL-1b in avian reovirus infected cells. 68 In cells infected with RSV, the stimulation of Akt activity leads to the expression of inflammatory cytokines such as interleukin-8 (IL-8) during infection. 57, 107, 108 EMCV also stimulates inflammatory cytokine production through the activation of the PI3K/Akt pathway. 109 Influenza-infected cells produce IL-8 and RANTES in a PI3K/Akt-dependent manner, 110 and virus activation of Akt potentiates the expression of interferon-b. 97 Influenza virus-infected macrophages can be kept alive by chemokine receptor-5mediated activation of the Akt pathway, which allows the infected cell to clear other infected cells. 111 Thus activation of Akt signaling can act as a double-edged sword during infection, promoting viral infection and replication and driving the expression of host danger signals that can recruit immune cells to the site of infection. Thus, though many viruses appear to activate the PI3K/Akt pathway, this activation also seems to carry with it some negative consequences. One approach to avoiding these negative consequences is to inactivate this signaling pathway upon infection. With the importance of Akt signaling in the host antiviral response, it comes as little surprise that some viruses appear to avoid the use of the PI3K/Akt pathway altogether and instead appear to inhibit its function. This is a stark departure from the many examples of viruses that activate the signaling pathway, but underscores that while activation is common, it is not a requirement for virus replication. The first example of virus inhibition of PI3K/Akt signaling was described for measles virus, a pediatric pathogen whose hallmark of infection is the suppression of immune function. This immune suppression occurs through the decreased proliferation and responsiveness of lymphocytes. 112, 113 While it is not fully understood how measles induces immune cell quiescence, recent studies point to a strong role of the PI3K/Akt pathway in this effect. Both in vivo and in vitro, the attachment of measles virus to the surface of T cells, can force Akt dephosphorylation in uninfected cells (Fig. 4) . This inactivation of PI3K/Akt signaling is not a consequence of infection but merely requires virus binding to a cell surface receptor. 114 The receptor that is responsible for signaling this downregulation is currently unidentified, but following the binding of measles virus particles to the cell surface, there is both a decrease in PI3K and Akt signaling and an upregulation of the inositol phosphatase SIP110 115 that lowers the overall level of PIP3 in the cell membrane. This promotes long-term suppression of PI3K/Akt signaling. The artificial stimulation of Akt activity overcomes measles-induced immunosuppression, suggesting that the inhibition of Akt is a central cause of measles virus-induced immune suppression. In addition to the effect of virus particle binding on Akt signaling, active infection of cells by measles virus continues to block Akt activity even after entry. This suppression of a central cell-viability pathway has no demonstrable negative effect on measles replication. 116 Thus, unlike most viruses (even related viruses such as RSV and HPIV5), measles replication appears to be insensitive to the activity of the PI3K/Akt pathway and does not require the antiapoptotic protection that could be afforded by activating this pathway. A different mechanism of Akt suppression is seen upon infection by another RNA virus, vesicular stomatitis virus (VSV). VSV is a veterinary pathogen that is being developed both as an oncolytic agent 117 and as a platform for effective vaccines. 118 VSV replication is unaffected by Akt signaling. 119 As is seen in measles virus, infection of cells with VSV results in a rapid inhibition of Akt signaling in infected insect 120 and mammalian cells. 121 This inhibition, which extends to inhibit the downstream components mTOR, 122 4E-BP1, 123 and rpS6, 124 makes cells insensitive to normal Akt-stimulating factors such as insulin and epidermal growth factor and is due in large part to the actions of the viral matrix protein (Fig. 4) . 121 Unlike measles, VSV does not block Akt activity through inhibition of PI3K signaling. Instead, the virus blocks the activating phosphorylation of Akt by inhibiting the phosphorylation of Akt at the plasma membrane through the actions of the viral matrix protein. The inhibition of PI3K/Akt signaling is also noted in cells infected with Rift Valley fever virus (RVFV). Recent phosphoproteome analysis of RVFV-infected cells showed that infection led to the dephosphorylation of the Akt-phosphorylated sites of FOXO1 and GSK3b. 125 The authors also observed a dephosphorylation of IRS1, an insulin-receptor adaptor that can facilitate PI3K/Akt activation. While the proteomics analysis did not identify Akt phosphorylation changes, the results are very much in line with RVFV inhibition of the Akt pathway. It is not clear how this virus evokes that inhibition of signaling at this point, but the analysis is consistent with the interpretation that it is downregulating RTK activity. Recent studies of alphaviruses show that these viruses also appear to downregulate the PI3K/Akt signaling pathway. Analysis of cells infected with the prototype alphavirus, Sindbis virus, showed that the virus downregulates PI3K/Akt activity. This downregulation of Akt phosphorylation had little effect on virus replication, and mTOR inhibitors did not significantly alter the replication of Sindbis virus. 126 This shows that alphaviruses are also capable of replicating without the contribution of these signaling pathways, which is directly analogous to what is seen in measles and VSV replication. The inhibition of PI3K/Akt signaling by Sindbis is likely relevant to the pathogenesis of alphaviruses, as these viruses are known to be neurotropic, and Akt activity is an important survival factor for these cells. 127 The startling regularity with which viruses appear to utilize the Akt pathway has not gone unnoticed in the antiviral drug discovery field. There have been many suggestions that Akt inhibitors or inhibitors of downstream effectors such as mTOR might be used as either specific or general antiviral compounds, targeting steps of the virus lifestyle ranging from fusion and entry 128 to transcription inhibition 60 to triggering cell death of infected cells. 40 However, given the important role of Akt in promoting immune cell responsiveness 114, 129 and the negative effect of PI3K/Akt inhibition on host immune cell function seen during measles virus infection, such an approach seems likely to dampen the host immune response. This latter conclusion is supported by reports showing that the PI3K/Akt pathway controls interferon production in dendritic cells 130, 131 and that it is important for controlling an autophagy-based antiviral response. 120 This suggests that such an approach is unlikely to succeed as a broad-spectrum approach. However, for viruses that activate PI3K/Akt signaling to maintain a latent infection such as the EBV, inhibiting signaling through this pathway appear to be successful in limiting downstream effects of latency, such as cellular transformation. 132 This suggests that there may be limited situations in which this approach will be successful. The PI3K/Akt signaling pathway itself may represent an evolutionary ''tugof-war'' for viruses. If the PI3K/Akt signaling pathway is just as essential for host response and survival as it is for viral function, inactivation is not an effective response for the infected host. Similarly, for viruses that have adapted to activate the pathway and that rely on the downstream effect of this activation, they must also contend with ''unwelcome'' results of such pathway activation, including the stimulation of cytokine production. This suggests that PI3K/Akt signaling is part of the evolutionary ''red queen race'' between virus and host, where virus attempts at controlling the host cell are often thwarted by host evolution. While it has long been recognized that viruses are wholly dependent upon the use of cellular machinery for macromolecular events such as protein synthesis, it is now clear that viruses additionally rely upon cellular phosphoproteins and signaling cascades to mold the cellular environment to their advantage. The PI3K/Akt phosphorylation cascade is an emerging paradigm for how these interactions occur. Many viruses have incorporated methods to activate PI3K/Akt signaling in order to forestall apoptosis, drive the cell cycle, and thereby support their replication processes. Somewhat paradoxically, other viruses have evolved an opposite approach, short-circuiting the PI3K/Akt signaling axis. In this latter case, viruses appear to benefit from the repression of the host antiviral response that comes from blocking Akt signaling. Why do different viruses, some from the same families, take different approaches? This is a question that will need to be answered in future research. It is likely that a better understanding of the importance of viral control of the PI3K/Akt signaling will be gained when the Akt substrates that are vital for viral replication are defined. Some significant progress has been made already. The phosphorylation of host factors such as the mTOR substrate 4E-BP is important for the robust translation of many viral messages, and it is likely that other Akt substrates that stimulate translation will also be sown to be important. It is likely that still more Akt-regulated phosphoproteins play a role in viral processes. Perhaps the most obvious but most poorly understood is the possibility that viral proteins themselves serve as substrates for Akt or effectors such as mTOR and GSK3. Recent work has suggested that Akt may phosphorylate the phosphoprotein of several negative-stranded RNA viruses 60 and that it may also control calicivirus polymerase function. 133 Future discoveries will likely greatly expand the number of viral substrates and more clearly point out the viral dependencies on this signaling axis. 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production Vesicular stomatitis virus infection alters the eIF4F translation initiation complex and causes dephosphorylation of the eIF4E binding protein 4E-BP1 Suppression of viral replication by stress-inducible GADD34 protein via the mammalian serine/threonine protein kinase mTOR pathway Reverse-phase phosphoproteome analysis of signaling pathways induced by Rift valley fever virus in human small airway epithelial cells Sindbis virus replication, is insensitive to rapamycin and torin1, and suppresses Akt/mTOR pathway late during infection in HEK cells Diversity in the mechanisms of neuronal cell death Reciprocal regulation of AKT and MAP kinase dictates virus-host cell fusion Differential effects of STAT5 and PI3K/AKT signaling on effector and memory CD8 T-cell survival PI3K is critical for the nuclear translocation of IRF-7 and type I IFN production by human plasmacytoid predendritic cells in response to TLR activation Toll-like receptormediated induction of type I interferon in plasmacytoid dendritic cells requires the rapamycin-sensitive PI(3)K-mTOR-p70S6K pathway Rapamycin inhibits the interleukin 10 signal transduction pathway and the growth of Epstein Barr virus B-cell lymphomas Norovirus RNA-dependent RNA-polymerase is phosphorylated by an important survival kinase The authors would like to thank Rachel Fearns and Erin Hodges for helpful conversations, Rahm Gummuluru for insight regarding retroviruses and lentiviruses, and Rebecca Connor for significant help with editing and revision. We apologize that a number of references could not be incorporated because of space consideration.