key: cord-0010060-brl7kmxs
authors: Iglesias, Gerardo; Pijoan, Carlos; Molitor, Thomas
title: Interactions of Pseudorabies Virus With Swine Alveolar Macrophages: Effects of Virus Infection on Cell Functions
date: 1989-05-01
journal: J Leukoc Biol
DOI: 10.1002/jlb.45.5.410
sha: 29b95b8d48965ad260d07c6ea4f266a33b9665c2
doc_id: 10060
cord_uid: brl7kmxs

In order to assess the effect of Pseudorabies virus (PRV) infection on the function of swine alveolar macrophages (AM), lung lavage cells were cultured, infected with one of six strains of PRV, and various activities were measured. Activity measurement included viability, phagocytosis of yeast, phagosome‐lysosome fusion, phagocytosis of opsonized particles, and superoxide release. AM were infected with 5 × 10(‐3) PFU/cell, and the comparative assessment of functions was performed at 18‐20 h postinfection. Cell viability in PRV‐infected cultures ranged from 79 to 94% of the viability in noninfected cultures. Phagocytosis of yeast was significantly reduced only in the AM cultures infected with the strain S‐62. Phagosome‐lysosome fusion was depressed in cultures infected with the strains S‐62, 4892, 3816, and BUK. The phagocytosis of opsonized sheep red blood cells showed significant differences between noninfected and PRV‐infected cultures in all cases except cultures infected with the strain PRV‐C. The O(2) release after stimulation with opsonized zymosan was significantly reduced in all the PRV‐infected cultures. The effect of PRV infection on AM functions that are related to the bacterial activity of such cells suggests that PRV‐induced AM dysfunction might have a role in the increased susceptibility of PRV‐infected pigs to bacterial pneumonia.

1.0 ml of 1% Triton X-100 was added to each well. The total contents of each well were transferred to a tube, and the radioactivity was counted in a gamma counter.

Each test was evaluated in triplicate.

PRY-infected or -noninfected AM cultured in siliconized tubes were pelleted by centrifugation at 600g x 10 mm and resuspended in 0. (Table  3) . The experiments performed with increasing virus input showed that the decrease in metabolic activity was virus input dependent (Fig. 2) . 

Viral-bacterial interactions in pulmonary infection