key: cord-0014869-kzbir081 authors: Martini, Veronica; Paudyal, Basu; Chrun, Tiphany; McNee, Adam; Edmans, Matthew; Atangana Maze, Emmanuel; Clark, Beckie; Nunez, Alejandro; Dolton, Garry; Sewell, Andrew; Beverley, Peter; MacLoughlin, Ronan; Townsend, Alain; Tchilian, Elma title: Simultaneous Aerosol and Intramuscular Immunization with Influenza Vaccine Induces Powerful Protective Local T Cell and Systemic Antibody Immune Responses in Pigs date: 2021-02-01 journal: J Immunol DOI: 10.4049/jimmunol.2001086 sha: 8e3d8673613b64fe2e0d38b2d6ddccf171d10499 doc_id: 14869 cord_uid: kzbir081 A vaccine providing both powerful Ab and cross-reactive T cell immune responses against influenza viruses would be beneficial for both humans and pigs. In this study, we evaluated i.m., aerosol (Aer), and simultaneous systemic and respiratory immunization (SIM) by both routes in Babraham pigs, using the single cycle candidate influenza vaccine S-FLU. After prime and boost immunization, pigs were challenged with H1N1pdm09 virus. i.m.-immunized pigs generated a high titer of neutralizing Abs but poor T cell responses, whereas Aer induced powerful respiratory tract T cell responses but a low titer of Abs. SIM pigs combined high Ab titers and strong local T cell responses. SIM showed the most complete suppression of virus shedding and the greatest improvement in pathology. We conclude that SIM regimes for immunization against respiratory pathogens warrant further study. A vaccine providing both powerful Ab and cross-reactive T cell immune responses against influenza viruses would be beneficial for both humans and pigs. In this study, we evaluated i.m., aerosol (Aer), and simultaneous systemic and respiratory immunization (SIM) by both routes in Babraham pigs, using the single cycle candidate influenza vaccine S-FLU. After prime and boost immunization, pigs were challenged with H1N1pdm09 virus. i.m.-immunized pigs generated a high titer of neutralizing Abs but poor T cell responses, whereas Aer induced powerful respiratory tract T cell responses but a low titer of Abs. SIM pigs combined high Ab titers and strong local T cell responses. SIM showed the most complete suppression of virus shedding and the greatest improvement in pathology. We conclude that SIM regimes for immunization against respiratory pathogens warrant further study. The Journal of Immunology, 2021, 206: 652-663. I mmunization against infectious diseases has been practiced for several centuries, but identifying the best method of administering a vaccine is still often a matter of empirical experimentation. Three major considerations should make rational immunization easier. The first is the importance of pathogenassociated molecular patterns, which are essential for triggering an immune response. The second is that the site of immunization programs lymphocytes to return to it. The third is that local immune responses are critical for protection against mucosal infection and that many lymphocytes reside in nonlymphoid tissues and provide tissue-resident memory. Immunization at the site of infection offers the advantage that an immune response is generated at the site of entry of the pathogen and should provide immediate protection. Immunization of the respiratory tract has been demonstrated to be highly effective against influenza, and cold-adapted, live attenuated influenza vaccine (LAIV) has efficacy rates of 75-80% in children and additionally gives some cross-reactive protection against antigenically distinct strains. However, LAIV is not so effective in adults or the elderly. In contrast, the traditional i.m.-inactivated seasonal human influenza vaccine provides 10-60% efficacy and induces strain-specific immunity by generation of subtype specific Abs so that repeated annual vaccination to match new influenza variants is required (1) (2) (3) . Therefore, there is an urgent need for new immunization strategies for influenza that provide broad and long-lasting protection. One such strategy, which has been explored against tuberculosis (Tb), is to combine the advantages of local and systemic immunization. Parenteral Bacillus Calmette-Guérin (BCG) priming followed by intranasal boosting with an adenovirus-vectored vaccine expressing Ag 85A (Ad85A) markedly enhanced protection in mice (4) . We have shown that simultaneous systemic and respiratory immunization (SIM) with BCG in mice or BCG/BCG and BCG/Ad85A in cattle enhanced protection against Tb challenge (5, 6) . Uddback et al. (7, 8) have used this strategy with an adeno vector expressing influenza nucleoprotein (NP) and shown greatly improved and durable protection against heterosubtypic influenza challenge in mice. These data prompted us to test SIM in the pig model using the candidate broadly protective signal minus influenza vaccine S-FLU. S-FLU is a pseudotyped influenza virus, *The Pirbright Institute, Pirbright GU24 0NF, United Kingdom; † Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom; lacking the hemagglutinin (HA) signal sequence and therefore limited to a single cycle of replication. S-FLU induces a strong cross-reactive T cell response but a minimal humoral response to HA when administered mucosally (9, 10) . We have shown that aerosol (Aer) delivery of S-FLU reduces lung viral load when partially matched to the challenge virus, correlating with a local lung T cell immune response (11) . When S-FLU was completely mismatched to the challenge virus, pathology but not viral load was reduced. This suggests that in the absence of an Ab response, lung T cell immunity can reduce disease severity (12) . By contrast, the same S-FLU preparation induced sterile immunity to the matched challenge virus and reduced replication and Aer transmission to naive recipients following mismatched viral challenge in ferrets (12) . The pig is a more relevant large animal model because it is a natural host for influenza viruses and has very similar respiratory anatomy to humans (13, 14) . Pigs and humans are infected by the same subtypes of influenza A viruses and are integrally connected in the ecology of influenza. In this study, we evaluated the efficacy of SIM with S-FLU against H1N1pdm09 challenge using inbred Babraham pigs, allowing a more refined analysis of the specificity of the immune responses using MHC class I tetramers to previously defined immunodominant NP epitopes (15) . The H1N1 signal minus influenza vaccine (S-FLU) [eGFP*/N1(A/Eng/ 195/2009)] H1(A/Eng/195/2009) containing the internal genes of A/Puerto Rico/8/1934 virus was produced as previously described (9) . The swine isolate H1N1 A/swine/England/1353/2009 (H1N1pdm09) was used to infect the pigs. The animal experiment was approved by the ethical review process at Animal and Plant Health Agency and followed the U.K. Government Animal (Scientific Procedures) Act 1986. Twenty-four 5-6-wk-old Babraham large, white, inbred female and male pigs were randomized into four groups of six animals as follows: 1) the first group received S-FLU by Aer as previously described (12) ; 2) the second group was immunized i.m. with S-FLU (i.m.); 3) the third group was immunized simultaneously i.m. and by Aer with S-FLU (SIM), and 4) the fourth group was an unimmunized control group. Two pigs reached their humane end points because of a pre-existing heart condition, limiting the number of pigs in the control and i.m. groups to five animals. During immunization, all the animals were sedated with a mixture of 4.4 mg/kg Zoletil (zolazepam) (Virbac) and 0.044 mg/kg Domitor (medetomidine) (Orion Pharma). Aer immunization was performed using a small droplet size, vibrating mesh nebulizer (Aerogen Solo; Aerogen) attached to a custom-made veterinary mask (12) . For Aer immunization, 2 ml of S-FLU containing 7 3 10 7 50% tissue culture infective dose (TCID 50 ) S-FLU was administered over 6-10 min. For i.m. administration, the vaccine stock was diluted to a final volume of 4 ml containing 7 3 10 7 TCID 50 , and 2 ml was administered to each trapezius muscle behind the ear. Pigs in the SIM group received 2 ml of 3.5 3 10 7 TCID 50 S-FLU by Aer (as described above) and 3.5 3 10 7 TCID 50 S-FLU delivered in 4 ml i.m. (2 3 2 ml in each trapezius muscle). The animals were boosted 3 wk later in a similar manner. Three weeks after the boost, all groups were challenged with 2.8 3 10 6 PFU of H1N1pdm09 intranasally using a mucosal atomization device (MAD300; Wolfe Tory Medical). For logistic reasons, the challenge was performed in two different days so that half of the animal in each group were challenged on 23 d postboost (DPB) and the remaining half on 25 DPB. Animals were humanely culled at 4 d postchallenge (DPC) with an overdose of pentobarbital sodium anesthetic. At the second cull, 1 mg/kg of anti-CD3 purified mAb (PPT3 clone, produced in-house) was infused i.v. to the pigs 10 min prior to sacrifice. Because no difference was found in analyses of the samples challenged on different days, the results are presented together. Gross and histopathological analyses were performed as previously described (11, 12, 16) . Briefly, the lungs were removed, and digital photographs were taken of the dorsal and ventral aspects. Macroscopic pathology was scored blind as previously reported (17) . The percentage of the lung displaying gross lesions for each animal was calculated using image analysis software (Fiji ImageJ) on the digital photographs. Lung tissue samples from cranial, middle, and caudal lung lobes were taken from the right lung and collected into 10% neutral-buffered formalin for routine histological processing. Formalin-fixed tissues were paraffin wax embedded, and 4-mm sections were cut and routinely stained with H&E. Immunohistochemical detection of influenza A virus NP was performed in 4-mm tissue sections as previously described (18) . Histopathological changes in the stained lung tissue sections were scored by a veterinary pathologist blinded to the treatment group. Lung histopathology was scored using five parameters (necrosis of the bronchiolar epithelium, airway inflammation, perivascular/bronchiolar cuffing, alveolar exudates, and septal inflammation) on a five-point scale of 0-4 and then summed to give a total slide score ranging from 0-20 per lobe (∼1.5 3 1.5 cm 2 ) and a total animal score from 0 to 60 (11) . A mean score for the three lung lobes was calculated for each animal. The individual lung lobes were also scored using the "Iowa" method, which takes into account the amount of viral Ag present in the sample, as described (19) . Blood, spleen, bronchoalveolar lavage (BAL), and lung lobes were processed as described previously (11, 12) . Trachea and nasal turbinate mucosae were separated from cartilage with tweezers and digested for 2 h at 37˚C in RPMI 1640 supplemented with 100 U/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine (all from Life Technologies), 2 mg/ml collagenase D (Roche), 1 mg/ml dispase, and 1 mg/ml of DNase (both from Sigma-Aldrich). Tissues filaments were then mashed with the plunger of a syringe. Isolated cells were then passed through a 70-mm cell strainer and RBC lysed before cryopreservation in FCS and 10% DMSO. Nasal swabs (one per nostril) were taken daily following infection with H1N1pdm09. Viral titer in nasal swabs and BAL was determined by plaque assay on MDCK cells as previously described (11) . ELISA was performed using recombinant HA (from A/England/195/2009) containing a C-terminal thrombin cleavage site, a trimerization sequence, a hexahistidine tag, and a BirA recognition sequence as previously described (20) . Microneutralization (MN) was performed using standard procedures as described previously (9, 16) . Enzyme-linked lectin assay (ELLA) was used to quantify neutralization of neuraminidase (NA) enzymatic activity by Ab as described before (21) . Briefly, NUNC Immuno 96-microwell plates (Sigma-Aldrich) were coated overnight at 4˚C with 25 mg/ml fetuin (Sigma-Aldrich) in PBS containing 0.02% sodium azide. Heat-inactivated sera and BAL were serially diluted in DMEM supplemented with 0.1% BSA, 100 U/ml penicillin, 100 mg/ml streptomycin, and 2 mM L-glutamine starting at 1:40 and 1:4, respectively. H7N1 S-FLU [eGFP/N1(A/Eng/09)] H7(Netherlands/219/2003) was used to minimize any potential steric effect of Abs binding to H1 HA. An optimal concentration of H7N1 S-FLU was added to the diluted Abs for 20 min on a plate shaker. One hundred microliters of the mixture of virus and diluted samples were then transferred to the washed coated plate and incubated for 18 h at 37˚C. Peanut agglutinin-conjugated with HRP (Sigma-Aldrich) was added at 1 mg/ml in PBS and incubated at room temperature for 2 h. The plates were washed and developed with 50 ml of TMB (Bio-Legend); after 5 min, the reaction was stopped with 50 ml of 1 M sulfuric acid and absorbance measured at 450 and 630 nm. The 50% inhibition titer was calculated as the highest dilution above the IC 50 line (midpoint between the signal generated by virus-only and medium-only wells). Cryopreserved lymphocytes from blood, spleen, and tracheobronchial lymph nodes (TBLN) were used. Then, 10 7 cells/well were stimulated in each well of a 12-well plate with the TLR7 agonist R484 at 1 mg/ml in RPMI 1640 supplemented with 100 U/ml penicillin, 100 mg/ml streptomycin, 10% FBS, and 0.1% 2-Mercaptoethanol (all from Life Technologies). After 48 h, cells were washed twice with medium and counted. Then, 5 3 10 5 cells were distributed in duplicate in assay plates for the detection of HA-specific Ab-secreting cells (ASC) and in negative control wells, whereas 0.5 3 10 5 cells per well were plated to detect all Ig-secreting cells (positive controls). Assay plates were MultiScreen-HA ELISpot plates (Millipore), coated with anti-porcine IgG, clone MT421 (Mabtech), or anti-porcine IgA, A100-102A (Bethyl Laboratories) 1/500 in carbonate buffer overnight at 4˚C. After overnight incubation at 37˚C, the plates were washed five times with PBS containing 0.05% Tween 20 and incubated with biotinylated HA for detection of HA-specific B cells (obtained as described before), biotinylated keyhole limpet hemocyanin (Sigma-Aldrich) as a negative control, both at 0.1 mg/ml in PBS, biotinylated anti-porcine IgG (MT424; Mabtech), or anti-porcine IgA (A100-102-B; Bethyl Laboratories) at 1/1000 in PBS to detect all Ig-secreting cells. After a 2-h incubation, plates were washed, and streptavidin alkaline phosphatase (Invitrogen) was added for another hour. The plates were then developed and read. Spots detected with keyhole limpet hemocyanin were subtracted from the HA response, and data were presented as ASC per million cells. Cryopreserved lymphocytes from BAL were thawed and stimulated with H1N1pdm09 (multiplicity of infection 1) or medium as a control for 18 h at 37˚C prior to GolgiPlug (BD Biosciences) addition as per manufacturer instructions. Following a 5-h incubation with GolgiPlug at 37˚C, cells were stained with surface markers (Table I ) before fixation and permeabilization using Cytofix Cytoperm (BD Biosciences). Intracellular staining was then performed, and the samples were analyzed using an LSRFortessa (BD Biosciences). Data were analyzed by Boolean gating using FlowJo v10 (Tree Star). For identification of tissue-resident memory T cells (TRM), three animals from each vaccinated group and two control animals were infused i.v. with 1 mg/kg of purified CD3 mAb (clone PPT3) and sacrificed 10 min later, as described above. Cryopreserved lymphocytes isolated from the different tissues were labeled with anti-mouse IgG1-allophycocyanin, which labels the circulating intravascular cells, for 20 min at 4˚C. After two washes with PBS, normal mouse serum was added to block any remaining binding sites of the secondary Ab. The lymphocytes were then stained with surface markers (Table I) , including anti-porcine CD3-FITC (clone PPT3; Bio-Rad Laboratories). As not all CD3 sites would be saturated by i.v. anti-CD3 mAb, circulating T cells are double labeled, whereas tissue-resident T cells are positive only for the ex vivo anti-CD3-FITC. NP tetramer staining was performed on cryopreserved lymphocytes from PBMC, lung, BAL, trachea, and nasal turbinate as previously described (15) . Briefly, biotinylated NP peptide-loaded swine leukocyte Ag monomers were freshly assembled into tetramer with streptavidin Brilliant Violet (BV) 421 or BV650 (both from BioLegend). Two million mononuclear cells were incubated with protease kinase inhibitor in PBS for 30 min at 37˚C, and tetramers were added to the cells on ice for another 30 min. Surface staining with optimal Abs concentration in FACS buffer (PBS supplemented with 2% FCS and 0.05% sodium azide) was performed on ice for 20 min (Table I) . Samples were washed twice with FACS buffer and fixed in 1% paraformaldehyde before analysis using an LSRFortessa (BD Biosciences). GraphPad version 8.4.1 was used for statistical analysis. Kruskal-Wallis test was used for the comparison between groups of viral load, pathology, Ab, and T cells responses. Two-way ANOVA was used for the comparison of neutralizing Ab and to analyze the hierarchy of the response in the different tissues within the same group. To evaluate the efficacy of simultaneous pulmonary and systemic immunization, groups of six inbred Babraham pigs were immunized with S-FLU expressing NA and coated in the HA from H1N1pmd09 i.m. or by Aer alone or simultaneously by Aer and i.m. (SIM). The SIM group received the same total dose as the i.m. or Aer groups but split between the two sites. Untreated pigs were used as controls. The animals were boosted 3 wk later and, after a further 3 wk, challenged with H1N1pdm09 virus and culled 4 d after the challenge (Fig. 1A) . Two pigs were culled before the end of the experiment because of underlying heart conditions, unrelated to the study, leaving five animals in the i.m. and control groups. Virus load was assessed in nasal swabs and BAL. The SIM pigs showed the greatest reduction of virus shedding in the nasal swabs at all time points except for the third DPC (Fig. 1B) . In the i.m. group, two individuals shed virus consistently after challenge, but a significant reduction in viral load was achieved on 1 DPC. Aer immunization did not decrease virus shedding, although two pigs did not shed at 4 DPC (Fig. 1B) . Overall i.m. and SIM significantly reduced the viral load in the nasal swabs over time, with an average area under the viral load/time curve of 3.46 and 2.23, respectively, compared with 9.53 and 11.46 of the Aer and control group (Fig. 1C) . No virus was detected in BAL at 4 DPC in any of the immunized groups (Fig. 1D) . The unimmunized animals showed typical gross pathology changes in the lungs with multifocal areas of consolidation in the cranial and medial lobes (Fig. 2A) . A significant reduction in the extension and severity of the gross changes was observed in the i.m. and SIM groups (p = 0.02 and p = 0.005, respectively, compared with controls), with a trend toward improved pathology in the Aer group that did not reach statistical significance (Fig. 2B) . A characteristic bronchointerstitial pneumonia, with bronchiolitis, alveolar exudation and lymphohistiocytic infiltration in the alveolar septa and peribronchial and perivascular areas was present in the unimmunized animals. A reduction in the severity of these changes was observed in the immunized groups (Fig. 2A) . Labeling of influenza A NP by immunohistochemistry (NP-IHC) was seen in only one animal in the i.m. and two animals in the SIM groups (p = 0.02 and p = 0.03, respectively), whereas most nonimmunized pigs displayed abundant labeling. NP-IHC was reduced in the Aer group, although this was NS (p = 0.55). Despite a reduction in gross lesions score and number of virus-infected cells, no significant difference was found when histopathology and NP-IHC were combined in all immunized groups (Iowa score) (Fig. 2B) . These results indicate that i.m. and SIM immunization significantly reduced nasal virus shedding and pathology, with SIM being more effective in virus clearance and gross lung pathology reduction. Aer immunization did not significantly reduce nasal virus load or pathology. All immunization regimes eliminated virus in the BAL. The serum-neutralizing titer against H1N1pdm09 in the i.m. and SIM groups increased after the boost to a peak of 4096 (50% inhibition titer) and 1812, respectively, at 14 DPB and declined by 22 DPB after the challenge (Fig. 3A ). The Aer group had a much lower peak serum-neutralizing titer of 54 at 14 DPB (Fig. 3A) . NA inhibition activity was assessed by ELLA at 4 DPC in the serum. i.m.-immunized animals showed the highest inhibition titer (1408) followed by SIM (453) and Aer (14.2) (Fig. 3A) . The neutralizing titer in the BAL was highest in the Aer group (13.4), and neutralizing activity was detectable in three of six SIM pigs (Fig. 3B) . There was no detectable neutralization in the BAL of i.m.-immunized animals, although HA-specific Abs were present. High levels of anti-HA IgG were present in both i.m. and SIM groups (119.2 and 167, respectively), whereas the highest titers of IgA were detected in the Aer and SIM groups (18.3 and 46.6, respectively) (Fig. 3B ). Only very low levels of NA inhibition were found in BAL compared with serum (Fig. 3B) . We also evaluated the number of memory IgG and IgA HA-specific B cells in spleen, TBLN, and blood. Very few ASC were found in the blood before challenge or in spleen at 4 DPC (Fig. 3C) . HA-specific, IgGsecreting ASC were detected in TBLN in the Aer (48 ASC/10 6 ) and in the SIM (25 ASC/10 6 ) groups. A lower number of HA-specific IgA ASC were present in these groups. Only two of five i.m.-immunized pigs had HA-specific IgG and IgA ASC in TBLN (Fig. 3C) . In summary, i.m. immunization with S-FLU induced high neutralizing Ab titers in serum but a limited response in BAL, although HA-specific Abs were present. Aer delivery generated the highest neutralizing titers in BAL but a very low serum response. The SIM group showed a high serum-neutralizing titer, although only half the magnitude of i.m. alone, whereas the BAL response was lower than in Aer only but still greater than i.m. Statistically significant numbers of HA-specific memory B cells were detected only in the Aer group in the local lung lymph nodes. We analyzed cytokine production of BAL T cells by intracellular staining following ex vivo stimulation with H1N1pdm09. No T cell response was detected in the BAL of the i.m. group. In contrast, Aer and SIM immunization induced a strong T cell response. CD8 T cells in the Aer and SIM groups secreted mainly IFN-g, followed by TNF, and the response in both groups was dominated by single IFN-g producers (51%) followed by double secreting IFN-g-TNF (36.2%) and a smaller proportion of triple secreting IFN-g-TNF-IL-2 cells (6.5%) (Fig. 4A) . The only significant CD4 responses were IFN-g in Aer and SIM groups, and there were few double or triple cytokine-producing cells (Fig. 4B) . Overall, Aer produced the strongest T cell response, dominated by IFN-g-producing cells. SIM induced similar T cells functions, although the response was slightly lower in magnitude. i.m. delivery did not generate virus-specific T cells in the BAL at 4 DPC. We enumerated S-FLU-specific CD8 T cells in blood and different parts of the respiratory tract (nasal turbinates, trachea, BAL, and lung) using three NP epitope tetramers: NP 290-298 DFEREGYSL (DFE), NP 101-109 NGKWMRELI (NGK), and NP 217-225 IAYERMCNI (IAY), as previously described (15) (Figs. 5, 6A , Table I ). No responses were detected against the previously identified NP 252-260 EFEDLTFLA epitope in all immunized animals (data not shown). In nasal turbinates, the response to IAY was the strongest (5% Aer, 1.4% i.m., and 3.4% SIM), followed by DFE (2.4% Aer, 0.7% i.m., and 1.4% SIM) and NGK (2.2% Aer, 0.2% i.m., and 1.5% SIM). The trachea showed similar specificity. The strongest response was detected in the BAL. NGK + CD8 T cells were the biggest population (13.4% in Aer and 13.8% in SIM), followed by IAY + (12.7% Aer and 9.4% SIM), and a lower response was found to DFE (6% Aer and 4.7% SIM). Strikingly, no tetramer staining was found in the BAL of the i.m. group, in agreement with the Each serum and BAL sample was assayed twice, and a mean computed. (C) HA-specific memory B cells were detected by ELISpot in PBMC (prechallenge), spleen, and TBLN 4 DPC. Each animal is represented by a symbol, and the mean is shown as a bar. Serum neutralization was analyzed with two-way ANOVA, whereas Kruskal-Wallis test was used for the analysis of NA neutralization in sera, BAL samples, and ELISpot data. Asterisks denote significance compared with control group (*p , 0.05, **p , 0.01, ***p , 0.001). lack of intracellular cytokine staining (Fig. 4) . In the lung, similar but lower tetramer-specific responses were detected for Aer (5.5% NGK, 7.5% IAY, and 3.5% DFE) and the SIM groups (7.9% NGK, 5% IAY, and 4.0% DFE) (Fig. 6A) . To evaluate the hierarchy of tetramer responses in different tissues, we calculated the proportion of each tetramer among total tetramer + CD8 T cells (Fig. 6B) . The proportions of IAY was much higher in all respiratory tissues compared with blood (p , 0.0001 for nasal turbinates compared with PBMC in the Aer group) (Fig. 6) . The NGK response in blood was greater compared with all respiratory tissues (p , 0.0001 when nasal turbinates were compared with blood for the Aer group). In the i.m. group, less NGK + cells were detected in all tissues compared with Aer and SIM. In particular, a significantly lower proportion of NGK + CD8 T cells was found in i.m. PBMC compared with Aer (p = 0.01), although they were still the dominant NP specificity among CD8 T cells (57.1% of total tetramer + CD8 T cells). Finally, we assessed the numbers of TRM in the respiratory tissues by i.v. infusion of anti-porcine CD3 mAb as previously described (12) (Fig. 7) . The majority of cells in the BAL were inaccessible to the mAb (82.4% average of all 11 animals treated with anti-CD3 mAb) and therefore tissue resident. In the nasal turbinates and trachea, 11.6 and 38.8% (average of all 11 animals) of single ex vivo-labeled CD3 cells (TRM) were detected, whereas in the lung, 95% of the T cells were double positive, perhaps reflecting known difficulties in extracting TRM and blood contamination (22) . Tetramer-positive cells were detected in both TRM and blood-borne populations. In summary, we detected strong NP tetramer-specific CD8 T cell responses in the nasal turbinates, trachea, BAL, and lung of Aerand SIM-immunized animals. i.m. induced much lower number of tetramer-specific cells in all tissues and none in BAL. There was a different hierarchy of the response specificity in the respiratory tract compared with the blood, indicating that sampling blood does not represent responses in the local tissues. In this study, we investigated different routes of immunization to determine the most effective in providing protection against influenza A virus in pigs. We tested the traditional systemic (i.m.) route used routinely in pigs and humans for influenza immunization and respiratory mucosal immunization, as used with the newer LAIV. We also performed SIM, previously shown to be highly protective, able to induce long-lasting immune response, and perhaps similar to prime and pull immunization regimes that have been investigated recently (5, 7, 8, 23) . In our experiments, we administered S-FLU to the whole respiratory tract, a procedure that has been shown in mice to be superior to upper respiratory tract immunization in protecting against heterologous challenge (24) . We were able to do so safely because S-FLU does not contain a viable RNA segment encoding HA. This obviates the two concerns that dictate restrictions of LAIV to the upper respiratory tract. First, the low level of replication of temperature-sensitive LAIV might cause lung pathology and, second, when used to protect against pandemic influenza viruses, reassortment of H5 or H7 with LAIV HA could occur (9, 11, 12, 25) . i.m. immunization induced a powerful neutralizing Ab response, and the viral load and lung pathology were both greatly reduced. However, T cell responses were weak in these animals and, strikingly, could not be detected in BAL at 4 DPC. Nor were neutralizing Abs present in BAL, although it should be noted that harvesting BAL involves considerable dilution, so low titers of Abs may be missed. These data indicate that i.m. immunization fails to develop lung responses, as previously reported in mice with seasonal human inactivated vaccine (26) . In contrast, in Aer animals, the serum-neutralizing titer was much lower, but neutralizing activity was detected in BAL. The viral load in nasal swabs was not reduced. There was a trend toward reduced gross and histopathology, although this did not reach significance. Aer animals made powerful CD8 and CD4 responses, detectable in BAL, a site containing almost exclusively TRM (12) . Given the powerful T cell and neutralizing Ab responses in the BAL, it is surprising that Aer animals showed minimal reduction in nasal virus shedding and a weak effect on pathology, although there was no virus in the BAL. Similarly, in a previous experiment, pigs immunized by Aer with H3N2 S-FLU and challenged with heterologous H1N1pdm09 virus exhibited reduced lung pathology 5 DPC but no reduction in virus shedding (12) . These data contrast with results in mice in which TRM have been shown both to protect against weight loss following heterologous influenza challenge and to reduce viral load (27) (28) (29) . This suggests that T cell immunity in mice can both protect against clinical disease (weight loss) and reduce viral load, whereas in pigs, a powerful T cells response is insufficient to protect the upper respiratory tract from infection and shedding, although the lung viral load and pathology are reduced. Parallel experiments using the same immunization regimes and challenge virus in pigs and ferrets confirm that small animal models may not always predict the outcome in a large animal natural host, such as the pig (12) . Correlative studies in humans suggest that cross-reactive T cells provide partial protection against influenza infection (30) (31) (32) . Taken together, these data suggest that in large animals, pigs, and humans, cross-reactive T cell immunity can alleviate severe disease but not prevent upper respiratory infection. The SIM animals showed a combination of the properties of the two immunizations. There was a good serum-neutralizing Ab response, and neutralizing Abs were also detected in BAL, but in contrast to i.m.-immunized animals, there was a powerful CD4 and CD8 T cell response in the BAL, lungs, and nasal turbinates. SIM animals showed greatly reduced viral load in nasal swabs and no detectable virus in BAL at 4 DPC together with reduced lung pathology. Our study shows that simultaneous systemic and Aer immunization may have the advantage of providing both protection against homologous challenge by induction of local and systemic neutralizing Abs and heterologous challenge, mediated by local and systemic T cells, including TRM. Systemic immunity may also be of benefit because influenza virus infection can also be systemic, and serum-neutralizing Abs may abolish viremia (33, 34) . A heterologous challenge of SIM animals would be extremely interesting and confirm whether this immunization regimen is truly advantageous. Simultaneous and prime pull immunization regimes have had mixed success. Although systemic prime followed by skin or reproductive tract pull successfully generated protective TRM in these tissues, it has been less easy to protect the respiratory tract by the same strategy (35, 36) . Our earlier experiments with BCG in mice and cattle showed that simultaneous parenteral and intranasal administration of BCG provided improved protection, which we attributed to earlier local control of mycobacterial replication in the lungs postchallenge (5, 6) . However, others have not replicated this in primates (37) . Similarly, immunization with an intranasal lentiviral Tb vaccine containing Ag 85A (pull) after BCG prime failed to improve protection (38) . In contrast, simultaneous immunization against influenza virus with an adenoviral vector in mice provided improved protection for up to 8 mo compared with s.c. or intranasal immunization alone, and TRM generated by this immunization regimen replicated in situ (7, 8) . These differing outcomes may be partly explained by the need for additional signals as well as the Ag. For example, an adenoviral but not vesicular stomatitis virus vectored pull improved anti-Tb protection in mice (39) , and an adenoviral vector encoding NP and 4-1BB ligand enhanced protection against influenza virus compared with NP alone (40) . In the current study, i.m. S-FLU generated very weak T cell responses, and the data provide little evidence for recruitment of systemic T cells to the respiratory tract following infection (pull). However, i.m. S-FLU induce a strong neutralizing Ab response, an essential component of influenza vaccines, but the effectiveness of the SIM regimen might be improved by a systemic immunization able to generate a stronger circulating memory T cell reservoir, important for the replenishment of local responses (41) . Although local immune responses are important and, when combined with systemic responses, may provide optimal protection, it is fundamental to know how long they persist. Lung TRM have been shown to be short lived in mice, perhaps as a consequence of the high oxygen tension of the lung microenvironment (41, 42) . However, experiments examining the persistence of influenza-specific memory indicate that a dividing lung memory population may persist for many months if Ag is retained in the lung (7) . Our data using Ad85A vaccine showed that lung cells from mice immunized 23 wk previously could stimulate 85A-specific T cells to divide, indicating long-term persistence of Ag (23) . Our data illustrate another factor that may partly explain why some regimes have not worked. Although we have not analyzed the entire T cell repertoire in detail, the different hierarchy of specificities of tetramer + CD8 T cells in i.m. compared with Aer animals suggests that the route of immunization can affect the T cell epitope specificity. Others have found that local cognate Ag recognition is fundamental for establishment of influenza-specific TRM (43, 44) and that local immunodominance is not always found in the circulating T cell pool, although it remains to be shown conclusively whether these differences in immunodominance affect protection (45) . Our results in this pig influenza challenge model indicate that SIM may offer advantages in protection against influenza viruses. SIM induces an excellent systemic Ab response, known to correlate with protection against homologous virus infection, as well as a powerful local TRM response, vital for protection against heterologous virus challenge. We suggest that development of SIM strategies for other respiratory pathogens, including SARS CoV-2, may be advantageous in providing both local protection and a high titer of Ab. SIM strategies should also take into account the need for local costimulatory signals and persistence of Ag in the lung. Efficacy and effectiveness of influenza vaccines: a systematic review and meta-analysis Live and inactivated influenza vaccines induce similar humoral responses, but only live vaccines induce diverse T-cell responses in young children Boosting of cross-reactive and protection-associated T cells in children after live attenuated influenza vaccination Intranasal boosting with an adenovirus-vectored vaccine markedly enhances protection by parenteral Mycobacterium bovis BCG immunization against pulmonary tuberculosis Protection induced by simultaneous subcutaneous and endobronchial vaccination with BCG/BCG and BCG/adenovirus expressing antigen 85A against Mycobacterium bovis in cattle Simultaneous immunization against tuberculosis Longterm maintenance of lung resident memory T cells is mediated by persistent antigen Combined local and systemic immunization is essential for durable T-cell mediated heterosubtypic immunity against influenza A virus Pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity Nonreplicating influenza A virus vaccines confer broad protection against lethal challenge Aerosol delivery of a candidate universal influenza vaccine reduces viral load in pigs challenged with pandemic H1N1 virus Comparison of heterosubtypic protection in ferrets and pigs induced by a singlecycle influenza vaccine Influenza A virus infections in swine: pathogenesis and diagnosis Swine as a model for influenza A virus infection and immunity Induction of influenza-specific local CD8 T-cells in the respiratory tract after aerosol delivery of vaccine antigen or virus in the Babraham inbred pig Establishment of a pig influenza challenge model for evaluation of monoclonal antibody delivery platforms Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus Interspecies transmission of reassortant swine influenza A virus containing genes from swine influenza A(H1N1)pdm09 and A(H1N2) viruses. Emerg Kinetics of lung lesion development and pro-inflammatory cytokine response in pigs with vaccineassociated enhanced respiratory disease induced by challenge with pandemic (2009) A/H1N1 influenza virus Focused antibody response to influenza linked to antigenic drift Broadly inhibiting antineuraminidase monoclonal antibodies induced by trivalent influenza vaccine and H7N9 infection in humans Quantifying memory CD8 T cells reveals regionalization of immunosurveillance Immunization of mice with a recombinant adenovirus vaccine inhibits the early growth of Mycobacterium tuberculosis after infection The contribution of systemic and pulmonary immune effectors to vaccine-induced protection from H5N1 influenza virus infection Small-particle aerosolization of live attenuated influenza vaccine virus Vaccine-generated lung tissueresident memory T cells provide heterosubtypic protection to influenza infection Cutting edge: tissue-retentive lung memory CD4 T cells mediate optimal protection to respiratory virus infection Lung-resident memory CD8 T cells (TRM) are indispensable for optimal cross-protection against pulmonary virus infection Airwayresident memory CD8 T cells provide antigen-specific protection against respiratory virus challenge through rapid IFN-g production Cellular immune correlates of protection against symptomatic pandemic influenza Natural T cell-mediated protection against seasonal and pandemic influenza. Results of the flu watch cohort study Cytotoxic T-cell immunity to influenza Delayed clearance of viral load and marked cytokine activation in severe cases of pandemic H1N1 2009 influenza virus infection Evidence of viremia in 2 cases of severe pandemic influenza A H1N1/09 Long-lived epithelial immunity by tissue-resident memory T (TRM) cells in the absence of persisting local antigen presentation A vaccine strategy that protects against genital herpes by establishing local memory T cells Prevention of tuberculosis in macaques after intravenous BCG immunization Using a prime and pull approach, lentivector vaccines expressing Ag85A induce immunogenicity but fail to induce protection against Mycobacterium bovis bacillus Calmette-Guérin challenge in mice Differentially imprinted innate immunity by mucosal boost vaccination determines antituberculosis immune protective outcomes, independent of T-cell immunity Intrinsic 4-1BB signals are indispensable for the establishment of an influenza-specific tissue-resident memory CD8 T-cell population in the lung Dynamics of influenza-induced lung-resident memory T cells underlie waning heterosubtypic immunity Environmental cues regulate epigenetic reprogramming of airway-resident memory CD8 + T cells Antibody-targeted vaccination to lung dendritic cells generates tissueresident memory CD8 T cells that are highly protective against influenza virus infection Pulmonary antigen encounter regulates the establishment of tissue-resident CD8 memory T cells in the lung airways and parenchyma Resident memory CD8 + T cells in the upper respiratory tract prevent pulmonary influenza virus infection We are grateful to the animal staff for excellent animal care. We thank the Pirbright flow cytometry facility for support and Kelly Roper and Emily Bessell for help with sample processing. We thank Animal and Plant Health Agency for providing the challenge swine A/Sw/Eng/1353/09 influenza virus strain (DEFRA SwIV surveillance programme SW3401).