key: cord-0015057-qxojyadk
authors: Saharman, Yulia Rosa; Karuniawati, Anis; Severin, Juliëtte A.; Verbrugh, Henri A.
title: Infections and antimicrobial resistance in intensive care units in lower-middle income countries: a scoping review
date: 2021-01-29
journal: Antimicrob Resist Infect Control
DOI: 10.1186/s13756-020-00871-x
sha: 78ced9b081991399002e08860aada81463b3d27f
doc_id: 15057
cord_uid: qxojyadk

BACKGROUND: Intensive care units (ICUs) in lower-middle income countries (LMICs) are suspected to constitute a special risk for patients of acquiring infection due to multiple antibiotic resistant organisms. The aim of this systematic scoping review was to present the data published on ICU-acquired infections and on antimicrobial resistance observed in ICUs in LMICs over a 13-year period. A systematic scoping review was conducted according to the PRISMA extension guideline for scoping reviews and registered in the Open Science Framework. MAIN BODY OF THE ABSTRACT: Articles were sought that reported on ICU-acquired infection in LMICs between 2005 and 2018. Two reviewers parallelly reviewed 1961 titles and abstracts retrieved from five data banks, found 274 eligible and finally included 51. Most LMICs had not produced reports in Q1 or Q2 journals in this period, constituting a large gap in knowledge. However, from the reported evidence it is clear that the rate of ICU-acquired infections was comparable, albeit approximately 10% higher, in LMICs compared to high income countries. In contrast, ICU mortality was much higher in LMICs (33.6%) than in high income countries (< 20%). Multidrug-resistant Gram-negative species, especially Acinetobacter baumannii and Pseudomonas aeruginosa, and Klebsiella pneumoniae played a much more dominant role in LMIC ICUs than in those in high income countries. However, interventions to improve this situation have been shown to be feasible and effective, even cost-effective. CONCLUSIONS: Compared to high income countries the burden of ICU-acquired infection is higher in LMICs, as is the level of antimicrobial resistance; the pathogen distribution is also different. However, there is evidence that interventions are feasible and may be quite effective in these settings. Protocol Registration The protocol was registered with Open Science Framework (https://osf.io/c8vjk)

Approximately fifty countries of the world belong to the category of lower-middle income countries (LMICs) according to the long-standing classification by the World Bank and updated every year [1] . These LMICs Page 2 of 19 Saharman et al. Antimicrob Resist Infect Control (2021) 10:22 stated in the report from the Wellcome Trust in 2014 [2] . Patients admitted to intensive care units (ICUs) are particularly at risk of acquiring infection due to multiple antibiotic resistant strains of notorious nosocomial pathogens including Enterococcus spp., Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli (a.k.a. the ESKAPE group of pathogens) [3] . A large international point prevalence survey on infections in the ICU conducted on May 8 th , 2007 included 1265 ICUs in 75 countries and provided insight in the prevalence and outcomes of such infections [4] . However, only eight LMICs participated in that survey and data on the occurrence and determinants of ICU-acquired infections and antimicrobial resistant pathogens from LMICs remain relatively rare and published wide apart. We, therefore, present here a scoping review of the data published on the infections and antimicrobial resistance observed in ICUs in LMICs over a 13-year period and published in esteemed scientific journals. We focused on revealing which LMICs have produced relevant information in this period, and which not, what type of ICU infections were observed and at what frequencies, which species and types caused these ICU-acquired infections, and present their antibiotic resistance profile. In addition, information was sought about the role of healthcare workers (HCWs) and the ICU environment, and whether intervention studies were performed and, if so, successful in reducing (risk of ) infections in these settings.

The scoping review protocol was developed as recently recommended by PRISMA extension for scoping reviews [5] [6] [7] and registered with Open Science Framework, an international prospective register of systematic scoping reviews on 13th December 2019 (https ://osf.io/c8vjk ) [5, 6] .

Any study that targeted the etiology and management of nosocomial bacterial infections in adult ICUs in LMICs, with a focus on antimicrobial resistance and interventions applied were eligible. Also, results of screening for multidrug-resistant bacterial pathogens (ESKAPE species) among humans (patients and HCWs) and the hospital environment were considered eligible for inclusion in this review. The population, intervention, comparison, and outcome (PICO) framework for determining the eligibility of the studies for the primary research question is presented in Table 1 .

We conducted a systematic scoping review of the epidemiology and management of multidrug-resistant bacteria in adult ICUs in countries classified as lower-middle year, lower-middle income economies were those with a GNI per capita between $1026 and $4035. Thus, low income countries were not included in this review. The term country refers to any territory for which authorities report separate social or economic statistics. The scoping review protocol was developed and registered in the Open Science Framework, an international prospective register of systematic scoping reviews, as recently recommended by PRISMA extension for scoping reviews [5, 6] . A systematic scoping review is a type of evidence synthesis method aimed at mapping the range of literature that exists around a specific topic of interest and focuses the research questions by charting the existing research findings and identifying research gaps. Scoping methodology is also considered a useful approach for determining the need and value of a future primary (indepth study) or a full systematic review [5] .

The review is restricted to papers published from January 1st 2005 until January 1st 2018, a time frame that is wide enough to allow all LMICs to contribute relevant data, and recent enough to still be relevant for today. We used EMBASE as the starting point and subsequently serially queried OvidSP 'Medline' , Cochrane, Web of Science and finally Google Scholar. The relevant literatures were identified using a single-line search strategy [8] with the following search strings:

('intensive care unit'/exp OR ((('intensive care' OR 'critical care') NEXT/1 unit*) OR icu OR icus):ab,ti) AND (infection/exp OR 'antibiotic resistance'/exp OR 'infection prevention'/exp OR 'infection control'/exp OR 'vancomycin resistant Enterococcus'/de OR 'methicillin resistant Staphylococcus aureus'/de OR 'extended spectrum beta lactamase'/de OR 'carbapenemase'/de OR 'Pseudomonas aeruginosa'/exp OR ' Acinetobacter baumannii'/exp OR (infection* OR sepsis OR septic OR nosocomial* OR mrsa OR ((multidrug OR multi-drug OR resistan*) NEAR/3 (bacter*)) OR ((vancomycin OR methicillin OR carbapenem) NEAR/3 resistan*) OR vre OR mrsa OR esbl OR (antibiotic* NEAR/3 resistan*) OR 'extended spectrum beta lactamase' OR 'extended spectrum β lactamase' OR 'Pseudomonas aeruginosa' OR ' Acinetobacter baumannii'):ab,ti) AND ((('lower middle' OR 'low middle' OR 'low-and middle') NEAR/6 income NEAR/3 countr*) OR lmic OR lmics OR Armenia* OR Mongolia* OR Bhutan* OR Morocc* OR Bolivia* OR Nicaragua* OR (Cabo NEXT/1 Verde*) OR Nigeria* OR 

TS = ((((("intensive care" OR "critical care") NEAR/1 unit*) OR icu OR icus)) AND ((infection* OR sepsis OR septic OR nosocomial* OR mrsa OR ((multidrug OR multi-drug OR resistan*) NEAR/3 (bacter*)) OR ((vancomycin OR methicillin OR carbapenem) NEAR/3 resistan*) OR vre OR mrsa OR esbl OR (antibiotic* NEAR/3 resistan*) OR "extended spectrum beta lactamase" OR "extended spectrum β lactamase" OR "Pseudomonas aeruginosa" OR "Acinetobacter baumannii")) AND ((("lower middle" OR "low middle" OR "low-and middle") NEAR/6 income NEAR/3 countr*) OR lmic OR lmics OR Armenia* OR Mongolia* OR Bhutan* OR Morocc* OR Bolivia* OR Nicaragua* OR (Cabo NEAR/1 Verde*) OR Nigeria* OR Cameroon* OR Pakistan* OR Congo* OR ("Papua New" NEAR/1 Guinea*) OR "Cote d Ivoire" OR Paraguay* OR Djibout* OR Philippin* OR Egypt* OR Samoa* OR Salvador* OR "Sao Tome and Principe" OR Georgia* OR Senegal* OR Ghan* OR "Solomon Islands" OR Guatemal* OR Guyana* OR (Sri NEAR/1 Lank*) OR Hondur* OR Sudan* OR India OR Swaziland* OR Indonesia* OR Syria* OR Kiribati* OR "Timor-Leste" OR Kosov* OR Ukrain* OR Kyrgyz* OR Uzbek* OR Lao OR laos OR Vanuatu* OR Lesotho* OR Vietnam* OR Mauritania* OR ("West Bank" NEAR/2 Gaza) OR Micronesia* OR Yemen* OR Moldova* OR Zambia*) NOT ((pediatric* OR picu OR nicu OR picus OR nicus))). 

"intensive|critical care"|icu|icus infection|infection s|nosocomial|mrsa|vre|esbl|"lower middle-income country|countries" |lmic|lmics|chine|egypt|indonesia|m orocco|phillippines|algeria|bolivia|colombia|ecuador|gu atemala|honduras|jamaica|nicaragua|thailand.

The references resulting from the Google Scholar data bank search were subsequently sorted by relevance, and only the first 200 references downloaded for inclusion [8] .

We followed the outlined stages of study selection guided by the aforementioned eligibility criteria (Fig. 2) . After retrieving by an experienced librarian, eligible papers (titles and abstracts) were exported to EndNote Library. The first author (YRS) screened all titles and abstracts and selected papers based on inclusion criteria. Another reviewer (HAV) independently performed a parallel review of titles and abstracts, and discrepancies between the two reviewers were resolved through consensus.

Subsequently, eligible papers published in journals ranked by their impact score as Q1 or Q2 in the Web of Science were selected for inclusion in the primary analysis. Full texts of the papers so selected were retrieved for full text review in a third round of screening for inclusion based on the criteria stated above, with reason for exclusion noted for each paper excluded on the basis of this full text review.

Papers excluded from the primary analysis based on the ranking of their journal of publication and those excluded during full text analysis were saved in separate files for potential analysis of specific questions arising during the remainder of the review process. Custom groups in EndNote were used to distinguish between various reasons for exclusion (Table 1) , and articles were assigned to specific groups for certain sub-questions. The reviewers (YRS and HAV) worked in their own copies of this library. After reading all articles, each reference in the library was discussed in detail; therefore, no automatic comparison was used and any discrepancy was resolved [7] .

Data were extracted by first author (YRS) and inputted into a data extraction table (Excel) and independently checked by the senior author (HAV) to ensure quality.

The extracted data comprised the characteristics of each study (first author name, year of publication, country, study period and design), characteristics of hospital and adult ICUs, population characteristics, the type and characteristics of adult ICU-associated infection, laboratory diagnosis, the total and individual number of the species (Gram-negative and Gram-positive) isolated from patients, HCW screening and environmental screening, their phenotypic and genotypic resistance characteristic, and the outcomes of patients (see Table 1 ).

Thematic analysis was performed to identify the current etiology and management of nosocomial bacterial infections in adult ICUs in LMICs from the included studies.

Where possible the results from the LMICs were compared with similar data collected from West-European countries in the same time frame. [4, [9] [10] [11] .

After duplicates were removed, a total of 1961 citations were identified from searches of electronic databases (Fig. 3 ). Based on the title and the abstract, 1687 were excluded, with 274 eligible articles published in journals ranked by their impact score by the Web of Science. Of these 274 articles, 93 were published in Q1 or Q2 journals and these 93 articles were subjected to a third round of eligibility check. Forty-two were excluded for specified reasons (see Fig. 3 for reasons of exclusion) and the remaining 51 papers were included in the primary analysis of this scoping review.

All included studies were carried out in LMICs and were published between 2005 and 2018. Fifty-one qualified studies were conducted in South Asia (India: 22 studies . 4) . Thus, the large majority of LMIC did not have information on ICU-associated infections published in Q1 or Q2 journals in this time frame. Most publications described surveillance and observational studies, only ten publications reported on intervention studies, either randomized or quasi-experimental in design. Multicenter studies were described in 28 publications. The characteristics of ICUs were not uniform, because some ICUs were highly specialized wards, including Burn Eleven studies presented the median of age of patients admitted, it ranged from 25 to 61 years, with a mean of the medians of 53 years. Twenty-three studies presented the mean age of patients admitted, it ranged from 32 to 71 years, with a mean of the means of 50 years. In the contemporary EPIC II study, the mean age of patients admitted to ICUs was 60.7 years [9] .

The overall frequency of ICU infections was presented using three types of calculations, as an attack rate in 11 reports, as point prevalence in eight and as incidence rate in seven, with some reports using multiple measures ( Table 2 ). The median lengths of stay were presented in 15 studies [13, 28, 30-32, 38, 47, 48, 50, 52, 54, 58-60, 62] , it ranged between 5 and 17 days. We calculated an overall median of the medians length of stay of 11 days, and an overall mean of the medians length of stay of 10 days. The overall in-ICU mortality rate extracted from 18 studies was 33.6% (1753/5241) patients. If we looked at individual studies, we found a wide range in recorded mortality rates varying between 14 and 70% [13, 17, 20, 25, 30-33, 37, 41, 46, 52, 54, 59, 60] .

Information on pathogens causing all ICU-associated infections was available from 11 studies [13, 16, 28, 32, 36, 43, 47, 50, 52, 58, 61] , six studies included microbiological data specifically related to VAP [13, 14, 26, 28, 58, 59] , seven had data related to CAUTI [13, 14, 28, 37, 56, 58, 59] and six had CLABSI data [13, 14, 21, 28, 58, 59] .

Gram-negative bacilli constituted the most prevalent group of nosocomial pathogens in these ICUs. The most common single pathogens causing ICU-acquired infection in LMICs were A. baumannii (24%), P. aeruginosa (16%), K. pneumoniae (15%), these caused the majority of infections. This distribution of pathogens is significantly different from the distribution of pathogens causing ICU-acquired infection in West-European countries in the same period, where these same three species caused < 25% of all infections [9] . In the European setting, Gram-positive pathogens were more prominent and the group of other nosocomial agents of ICU infection was larger (Fig. 5) .

A. baumannii was the most frequent pathogen identified for ventilator-associated pneumonia causing 42% of VAP, followed by P. aeruginosa which caused 25% of the VAP. Thus, these two species were involved in two thirds of all episodes of VAP in LMICs (Fig. 6 ). In contrast, K. pneumoniae was the dominant species in CLABSI, causing 24% of the episodes, as much as the combined impact of A. baumannii and P. aeruginosa. Together, the ESKAPE species were involved in two thirds of all CLABSI episodes. ESKAPE species also caused 51% of CAUTI in this setting, with E. coli as the most prevalent representative species. However, a sizable minority of CAUTI were caused by other species of uro-pathogenic microorganisms including many episodes that were caused by Candida species (data not shown).

Phenotypic resistance profiles of ESKAPE isolates to various antibiotics was determined in 15 studies. However, these studies were reported from only six LMICs, and were sometimes lacking data on certain combinations of ESKAPE species and classes of antimicrobial agents. Almost all isolates from LMICs were resistant to multiple classes of antibiotics, a condition that closely resembles the resistance patterns observed in most so-called Mediterranean countries, including Italy and Greece, located in the southern part of West-Europe (Table 3) . Compared to isolates from LMICs, the same species isolated from invasive infections in Nordic countries of West-Europe, including Sweden and the Netherlands, displayed much lower levels of antibiotic resistances (Table 3) . Vancomycin resistance among Enterococcus species was > 50% in Vietnam [50] and MRSA (methicillin-resistant Staphylococcus aureus) was identified in > 50% of all S. aureus isolates in most LMICs [28, 30, 35, 50, 59] . Multidrug resistant K. pneumoniae, A. baumannii, and P. aeruginosa were found among > 50% of the isolates in India, Pakistan, Egypt, Vietnam and Nigeria [13, 20, 27, 28, 35, 40, 42, 47, 52] .

Only a very few studies presented genetic information regarding the antibiotic resistances observed. Amissah et al. from Ghana reported that 28% of isolates of S. aureus tested positive for the mecA gene [60] . [27] more recently reported the presence of A. baumannii in 17/26 (65%) samples of humidifier water, and in 3/6 (50%) heat and moisture exchangers cultured in their ICU in a tertiary care center in South India. These environmental isolates showed the same multidrug resistance pattern as contemporary isolates from patients admitted to the ICU.

In Morocco, Uwingabiye et al. [46] identified 36 environmental A. baumannii isolates and compared them with 47 clinical isolates of the same species. They showed genetic similarity between the clinical and environmental isolates since 80/83 (96.4%) of all isolates belonged to the same 7 pulsed-field gel electrophoresis pulsotypes. Saharman et al. [54] likewise found six isolates of carbapenem-non-susceptible A. baumanniicalcoaceticus complex in the environment of two ICUs in a tertiary care center Indonesia, four of these isolates belonged to same dominant clone, defined by multilocus sequence typing, as those infecting their patients. Level of resistance: Data from indicated European Union countries were derived from reference [11] . Colors indicate increasing levels of resistance as specified in the legend, and blank boxes indicate that no data was available for the particular combination of species and antimicrobial agent 

HCWs may be another source of nosocomial pathogens, thus HCW screening may be an important measure to detect and eradicate such sources of antimicrobial resistance. However, only two studies addressing HCW carriage of resistant pathogens were available from LMICs in this time frame, one from Indonesia [54] and one from Ghana [60] . Saharman et al. [54] identified one HCW in their ICUs that carried a strain of carbapenem-non-susceptible A. baumannii-calcoaceticus complex, and Amissah et al. [60] found colonization with S. aureus isolates that were obtained from 13/29 (45%) of their HCWs, but only one of which carried MRSA.

We identified 10 publications that described interventions aimed to reduce ICU-acquired infections and antimicrobial resistances; all but one applied a quasi-experimental design to measure the effects of their intervention (Table 4 ) [18, 21-24, 33, 34, 48, 49, 56] . Multimodal strategies (those with ≥ 3 components implemented in an integrated manner to achieve improved outcomes and change behavior as defined by WHO guidelines) were used in most studies [63] . Outcomes were either processes, especially hand hygiene (HH) practice, in five studies or they were actual rates of ICU-acquired infections in seven studies (two studies had both types of outcomes, Table 4 ). Thu et al. (2015) in Vietnam performed a cost-effectiveness study analyzing the impact of a HH improvement program in ICUs. The study used the steps recommended by the WHO, including upgrading HH facilities, training, surveillance, and feedback. The study showed that HH compliance increased from 25.7 to 57.5% and the incidence of HAI decreased from 31.7 to 20.3% (p < 0.001) after the intervention; similar results were shown in several reports from India [18, 23, 24] .

Successful interventions have also targeted CLABSI, VAP, and CAUTI. The implementation of a multidisciplinary approach for prevention of VAP in ICUs in Pakistan [34] yielded a reduction from 18 to 13% in the VAP rate, and in India [22] VAP incidence decreased from 17.4 to 10.8 per 1000 ventilation days. In 16 ICUs in India a similar intervention strategy for CLABSI also showed a reduction in CLABSI incidence rates from 6.4 to 3.9 per 1000 central line days [21] .

Finally, Navoa-Ng et al. in the Philippines targeted CAUTI and reported a reduction of CAUTI from 11.0 to 2.66 per 1000 urinary catheter days as a consequence of applying an infection prevention bundle together with education, monitoring and feedback [56] .

Sixty-three papers were published in journals listed as Q3 or Q4 by the Web of Sciences but only nine [64] [65] [66] [67] [68] [69] [70] [71] [72] of those met our inclusion criteria after full text review. Those nine papers described six independent studies, all emanating from the countries already included in our primary analysis. The data extracted from those publications did not add novel information nor significantly changed the findings from our review of the information presented in our primary analysis. Specifically, the infection rates in these nine studies all fell within the range found in our primary analysis. In addition, only one paper from India [64] presented resistance rates of ESKAPE organisms; these rates all fell within the categories specified for India in Table 3 . However, this paper also had resistance rates for E. coli against aminoglycosides, fluoroquinolones, and 3 rd generation cephalosporins, all were > 50%. Of note, Ikeh et al. reported [73] MRSA contamination of instruments and surfaces in an Nigerian ICU, and Joseph et al. [69] found evidence that some strains of P. aeruginosa and A. baumannii were shared between the ICU environment and patients, and one of 16 HCWs carried a P. aeruginosa strain that was shared with a patient that had developed VAP.

In this systematic scoping review, we have shown that endemic nosocomial infections represent a major burden and safety issue for patients admitted to intensive care in lower-middle income countries. Unfortunately, there were relatively few studies on this topic published from LMICs in a highly ranked scientific journals (Q1/Q2 by Web of Science). From 50 LMICs, we only identified 51 qualified published studies performed in 11 LMICs over a thirteen-year time frame. Supplementing information from studies published in Q3/Q4 journals was not helpful since only nine additional papers published in these journals met our inclusion criteria, and they did not expand the areas already covered. There is, thus, a great unmet need in most LMICs to perform surveillance of ICU-acquired infections and to characterize the nosocomial pathogens involved. Such data are needed in order to obtain a more comprehensive view and monitor the problems of LMICs to control ICU-acquired infection and combat resistance to antimicrobial agents in their settings.

The ICU-acquired infection rates were quite high in LMICs, with an average point prevalence rate of 22.4 infected patients per 100 present in the ICU. This rate is comparable, albeit somewhat higher, to the average point prevalence rate of 19.5% recorded in ICUs across West-European countries in the same time frame (2011-2012) [4] . The device-associated infection indices were also comparable to those recorded in West-European ICUs at that time, 9.5 VAP/1000 intubation days, 3.3 CLABSI/1000 days with central line and 4.5 CAUTI/1000 days with urinary catheter [10] . Thus, the overall impression is that ICU-acquired infections in LMICs are quite similar in their nature, but that rates are somewhat higher (approximately 15%) in LMIC ICUs compared to ICUs in West-European countries. ICU length of stay and ICU mortality are important outcomes of intensive care. In studies retrieved by our search, the overall length of stay was 10-11 days and the overall ICU mortality rate was 33.6% (varying from 14 to 70% across the studies). In the same time frame in European countries, based on ICU surveillance from 2008 to 2012, the median (IQR) length of stay was 10 (8-12) days, which was highly comparable to the length of stay in LMICs [10] . However, mortality rates differed significantly. On average, 15.3% of EU patients staying more than two days died in the ICU, ranging from 8.7% in Luxembourg to 18.1% in France [10] . The Extended Prevalence of Infection in Intensive Care (EPIC II) study (2007) involving 1265 ICUs and 75 countries found an overall ICU mortality rate of 18.2% (2370/13,011 patients). Infected patients had higher ICU mortality rates (25.3%) and longer ICU lengths of stay (16 days [IQR, ) [9, 10] . Thus, the overall ICU mortality rate of 33.6% retrieved in this scoping review was much higher in LMICs, indicating that, compared to high income countries, patients in ICUs in LMICs die at a higher rate and that death comes relatively early during their ICU stay. The fact that in LMICs the mean age of ICU patients was much lower than in high income countries (50 years versus 60 years, respectively) further underscores the major discrepancy in ICU survival between these two groups of countries.

Gram-negative bacteria were responsible for more than 50% of the total number of ICU-acquired infections recorded in LMICs. This species distribution contrasts with findings from studies done in West-Europe at that time where the prevalent cause of healthcare-associated infections had switched over to Gram-positive microorganisms (72.7%) (EPIC II study) [9] . The microorganisms most frequently isolated from ICU infections in a later study [4] [4] . Especially the proportion of infections caused by Acinetobacter spp. in ICUs in LMICs was more than six times higher compared to West-European countries (24% versus 3.6%) [9] .

The ESKAPE group of pathogens will be of increasing relevance to antimicrobial chemotherapy in the coming years. Our findings revealed a high rate of multidrug-resistant (MDR) Gram-negative bacilli causing ICU infections in LMICs. The high proportions of strains resistant to third generation cephalosporins and of multidrug resistance among Gram-negative bacteria are especially worrisome. Comparably high rates of MDR among Gram-negative bacilli isolated from patients with invasive infections have been reported from Italy, Greece and some in France (EARS-Net by 30 EU/EEA countries in 2014) [11] . In contrast, much lower MDR rates among Gram-negative bacilli were observed from invasive infections in Sweden and the Netherlands [11] . The high percentages of resistance to carbapenems of P. aeruginosa, A. baumannii and K. pneumoniae isolates found in this scoping review reflect the challenges of treatment of ICU patients in LMICs. Although not reported in the studies included in this review the determinants of antimicrobial resistance in LMICs are likely to include a high selection pressure due to overconsumption of antibiotics and the lack of barriers against the spread of selected resistant clones in healthcare settings.

The implementation of a multidisciplinary approach for prevention of HAIs in ICUs from LMICs showed that reductions in the HAI rate are possible in LMICs. Some studies reported effective interventions including contact precautions, active surveillance cultures, monitoring, audit and feedback of preventive measures, patient isolation or cohorting, HH improvement programs, and environmental cleaning. This is also highlighted by the recent evidence-based WHO Guidelines on the core components of IPC programs, which strongly recommend multimodal strategies to translate IPC measures into clinical practice [63] .

One of the most comprehensive guidelines is the 2013 European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Guidelines for the management of infection control measures to reduce transmission of multidrug-resistant (MDR) Gram-negative bacteria [74] . In endemic settings, HH and contact precautions were the only two interventions that were strongly recommended for all three pathogens (MDR-K. pneumoniae, MDR-P. aeruginosa, and MDR-A. baumannii) in addition to isolation for MDR-K. pneumoniae and isolation, alert codes, education, and environmental cleaning for MDR-A. baumannii. In epidemic settings, hand hygiene, contact precautions, active screening, isolation and, last but not least, environmental cleaning are strongly recommended for all three pathogens in addition to alert codes and cohorting for MDR-K. pneumoniae [74] . Interestingly, implementation of HH best practices and environmental cleaning was reported in only few studies in LMICs so far. Effective HH compliance is widely recognized and strongly recommended by WHO to reduce transmission of pathogenic microorganisms in healthcare. Likewise, the important role of the innate environment of the ICU providing sources and routes of transmission of MDR microorganisms is gaining recognition worldwide. This scoping review revealed that implementation of these guidelines is essentially possible in LMICs, and are sorely needed to reduce the high burden of disease caused by ICU-acquired infections in these settings. Much room for further high quality observational and interventional research remains that should include more countries with a LMIC status, and target novel interventions that are cost-effective in this particular setting. The ICU cannot be rendered sterile but every effort should be made to reduce the number of ICU-acquired healthcare-associated infections (HAI) and the risk of spread of resistant nosocomial pathogens. Strategies to minimize infection have been incorporated into various guidelines on ICU design that are available in the UK, the USA and Europe [75, 76] . An ICU should accommodate at least 6 beds with 8-12 beds considered as the optimum. Hospitals with several smaller units should be encouraged to rearrange these units into a single larger department to improve efficiency. A larger ICU may provide opportunities to create separate, specialized functional subunits with 6-8 beds, sharing the same geographical, administrative, and other facilities [75, 76] . However, of those included in this review most ICUs in LMICs still had open ward designs with one large room, with beds separated by curtains only, if at all, they did not have separate cubicles or separate isolation rooms. The numbers of beds ranged between 4 and 75 beds. These open ICU designs are not optimal, they compare unfavorably with the current trend to construct ICUs as a series of separate rooms to better protect patients against ICU-acquired infections [75] . Thus, the roles of the environment and of HCWs in the endemicity and transmission of nosocomial pathogens in ICU settings should be further studied and delineated, they should no longer be underestimated. Also, not all ICUs in LMICs had dedicated and qualified intensivists; however, most of them did have multidisciplinary teams in charge of the patients (data not presented).

A limitation of this review is posed by the relatively low number of qualified studies that were performed in only a minority of the 50 countries belonging to the group of LMICs. We also restricted our review to publications in the English language. Although the vast majority of medical and healthcare research is published in English, we may have missed important information from researchers that elected to publish their data in another language.

Thus, this review cannot be taken to reflect the full scope of ICU-acquired infections in all LMICs, but from our perspective this currently represents the best available view on infections acquired in ICUs and the species and resistance profiles of the organisms causing such infections in LMICs.

Our systematic scoping review describes the current evidence of ICU-acquired infections in LMICs. Many gaps in knowledge remain since most LMICs have not produced high quality reports. However, from the reported evidence it is clear that the rate of ICU-acquired infections is likely to be somewhat higher in LMICs compared to high income countries and that the ICU mortality rate is much higher. MDR Gram-negative bacilli, especially Acinetobacter spp. and Pseudomonas spp. from the environment clearly play a much more dominant role in LMICs than in high income countries. However, interventions to improve this situation have been shown to be feasible and effective, even cost-effective.

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Prospective surveillance of device-associated health care-associated infection in an intensive care unit of a tertiary care hospital in New Delhi India

Clinical impact and cost-effectiveness of split-septum and singleuse prefilled flushing device vs 3-way stopcock on central line-associated bloodstream infection rates in India: A randomized clinical trial conducted by the International Nosocomial Infection Control Consortium (INICC)

Infection control education: impact on ventilator-associated pneumonia rates in a public sector intensive care unit in Pakistan

Surveillance of deviceassociated infections in intensive care units of a tertiary care hospital

Microbial pattern and antimicrobial resistance, a surgeon's perspective: retrospective study in surgical wards and seven intensivecare units in two university hospitals in Cairo Egypt

Surveillance of catheter-associated urinary tract infection in 4 intensive care units at Alexandria university hospitals in Egypt

Device-associated nosocomial infection rates in intensive care units at Cairo University hospitals: first step toward initiating surveillance programs in a resource-limited country

Device-associated infection rates in adult and pediatric intensive care units of hospitals in Egypt. International Nosocomial Infection Control Consortium (INICC)

Emergence of carbapenem-resistant Acinetobacter baumannii harboring the OXA-23 carbapenemase in intensive care units of Egyptian hospitals

Incidence and pathogen distribution of healthcare-associated infections in pilot hospitals in Egypt

The emergence of a novel sequence type of MDR Acinetobacter baumannii from the intensive care unit of an Egyptian tertiary care hospital

Health care-associated infections in pre-transplant liver intensive care unit: Perspectives and challenges

National surveillance of health care-associated infections in Egypt: Developing a sustainable program in a resource-limited country

Prevalence of hospital-acquired infection in a Moroccan university hospital

Clonal diversity and detection of carbapenem resistance encoding genes among multidrug-resistant Acinetobacter baumannii isolates recovered from patients and environment in two intensive care units in a Moroccan hospital

Need for improved antimicrobial and infection control stewardship in Vietnamese intensive care units

Effects of infection control measures on acquisition of five antimicrobial drug-resistant microorganisms in a tetanus intensive care unit in Vietnam

Cost-effectiveness of a hand hygiene program on health care-associated infections in intensive care patients at a tertiary care hospital in Vietnam

Burden of hospital acquired infections and antimicrobial use in Vietnamese adult intensive care units

A one-year prospective study of colonization with antimicrobial-resistant organisms on admission to a Vietnamese intensive care unit

Patterns of antimicrobial resistance in intensive care unit patients: a study in Vietnam

Surveillance of healthcare-associated infections in Indonesian hospitals

Endemic carbapenem-nonsusceptible Acinetobacter baumannii-calcoaceticus complex in intensive care units of the national referral hospital in Jakarta

Device-associated infections rates in adult, pediatric, and neonatal intensive care units of hospitals in the Philippines: international nosocomial infection control consortium (INICC) findings

Impact of an International Nosocomial Infection Control Consortium multidimensional approach on catheter-associated urinary tract infections in adult intensive care units in the Philippines: International Nosocomial Infection Control Consortium (INICC) findings

Multicenter study of device-associated infection rates in hospitals of Mongolia: findings of the international nosocomial infection control consortium (INICC)

Nosocomial infections and resistance pattern of common bacterial isolates in an intensive care unit of a tertiary hospital in Nigeria: A 4-year review

Incidence, clinical outcome and risk factors of intensive care unit infections in the Lagos university teaching hospital (LUTH)

Epidemiology of Staphylococcus aureus in a burn unit of a tertiary care center in Ghana

Prevalence of nosocomial infections in high-risk units in the university clinical center of Kosova

Prevalence of nosocomial infections in adult intensive care units at a Kosova Teaching Hospital

Guidelines on core components of infection prevention and control programmes at the national and acute health care facility level

Antibiotic resistance & pathogen profile in ventilator-associated pneumonia in a tertiary care hospital in India

Multidrug-resistant bacteria among patients with ventilator-associated pneumonia in an emergency intensive care unit

Epidemiology of nosocomial infections in medicine intensive care unit at a tertiary care hospital in northern India

Ventilator-associated pneumonia in a tertiary care hospital in India: incidence and risk factors

Ventilatorassociated pneumonia in a tertiary care hospital in India: Role of multidrug resistant pathogens

Role of intensive care unit environment and health-care workers in transmission of ventilator-associated pneumonia

Deviceassociated infections at a level-1 trauma centre of a developing nation: impact of automated surveillance, training and feedbacks

Bloodstream infections in NNICU: Blight on ICU stay

Pattern of nosocomial infection in two intensive care units of a tertiary care hospital in Karachi

Bacterial flora of fomites in a Nigerian multi-disciplinary intensive care unit

ESCMID guidelines for the management of the infection control measures to reduce transmission of multidrug-resistant Gram-negative Page

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Intensive care unit design and environmental factors in the acquisition of infection

Group on Quality Improvement. Recommendations on basic requirements for intensive care units: structural and organizational aspects

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We are thankful to The Directorate General of Higher Education of Indonesia Ministry of Research, Technology and Higher Education of the Republic of Indonesia, Dean of Faculty of Medicine Universitas Indonesia, Board of Directors of Dr. Cipto Mangunkusumo National General Hospital Jakarta Indonesia,