key: cord-0018981-fvfqjzyo
authors: Uddin, Md Josim; Russo, Daniela; Rahman, Md Mahbubur; Uddin, Shaikh Bokhtear; Halim, Mohammad A.; Zidorn, Christian; Milella, Luigi
title: Anticholinesterase Activity of Eight Medicinal Plant Species: In Vitro and In Silico Studies in the Search for Therapeutic Agents against Alzheimer's Disease
date: 2021-06-25
journal: Evid Based Complement Alternat Med
DOI: 10.1155/2021/9995614
sha: dc32aef14a5f6b1faaf97bf1c1a50ad6d073099a
doc_id: 18981
cord_uid: fvfqjzyo

Many Bangladeshi medicinal plants have been used to treat Alzheimer's disease and other neurodegenerative diseases. In the present study, the anticholinesterase effects of eight selected Bangladeshi medicinal plant species were investigated. Species were selected based on the traditional uses against CNS-related diseases. Extracts were prepared using a gentle cold extraction method. In vitro cholinesterase inhibitory effects were measured by Ellman's method in 96-well microplates. Blumea lacera (Compositae) and Cyclea barbata (Menispermaceae) were found to have the highest acetylcholinesterase inhibitory (IC(50,) 150 ± 11 and 176 ± 14 µg/mL, respectively) and butyrylcholinesterase inhibitory effect (IC(50), 297 ± 13 and 124 ± 2 µg/mL, respectively). Cyclea barbata demonstrated competitive inhibition, where Blumea lacera showed an uncompetitive inhibition mode for acetylcholinesterase. Smilax guianensis (Smilacaceae) and Byttneria pilosa (Malvaceae) were also found to show moderate AChE inhibition (IC(50,) 205 ± 31 and 221 ± 2 µg/mL, respectively), although no significant BChE inhibitory effect was observed for extracts from these plant species. Among others, Thunbergia grandiflora (Acanthaceae) and Mikania micrantha (Compositae) were found to display noticeable AChE (IC(50), 252 ± 22 µg/mL) and BChE (IC(50), 314 ± 15 µg/mL) inhibitory effects, respectively. Molecular docking experiment suggested that compounds 5-hydroxy-3,6,7,3′,4′-pentamethoxyflavone (BL4) and kaempferol-3-O-α-L-rhamnopyranosyl-(1⟶6)-β-D-glucopyranoside (BL5) from Blumea lacera bound stably to the binding groove of the AChE and BChE by hydrogen-bond interactions, respectively. Therefore, these compounds could be candidates for cholinesterase inhibitors. The present findings demonstrated that Blumea lacera and Cyclea barbata are interesting objects for further studies aiming at future therapeutics for Alzheimer's disease.

Alzheimer's disease (AD) is a devastating neurodegenerative disorder linked with the two most common symptoms, memory dysfunction and cognition impairment. In the neuropathological symptoms of AD, cognitive deficit is consistent with the presence of cholinergic deficit, due to the degeneration or atrophy of cholinergic neurons in the basal forebrain, including senile plaques and neurofibrillary tangles [1] . Acetylcholine (ACh), the brain's important natural neurotransmitter, plays a critical role in forming memories, verbal and logical reasoning, and the ability to concentrate. However, ACh's activity is greatly hindered by both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes.

Inhibition of the cholinesterase enzymes (AChE and BChE) can boost ACh levels in several parts of the brain and symptoms associated with the progressive loss of cholinergic function in AD improve [2] . Studies also showed that increased ACh concentration in the brain increases the expression of nicotinic ACh receptors related to cognitive function [3, 4] . is phenomenon might help AD patients generating new memories and remembering the older ones.

us, AChE and BChE inhibition has been established as a primary therapeutic target based on this "cholinergic hypothesis."

Pharmacological treatment of AD is largely based on treating symptoms and severity with advanced stages rather than targeting the etiological mechanisms. e currently approved medications for AD are mostly cholinesterase inhibitors, including donepezil, rivastigmine, galantamine, and NMDA antagonist memantine [5] . Among the commonly prescribed AChE inhibitors, only donepezil was approved for the treatment of all stages of AD. However, this drug has some severe side effects, including gastrointestinal disturbance, liver-associated problems, and GIT-related abnormalities [6] . Considering all these limitations, it is worthy to find new lead compounds from different sources, including plant-derived natural products.

Natural products have already proven to be promising sources of useful acetylcholinesterase (AChE) inhibitors [7] . e currently approved drugs for AD, galantamine and rivastigmine, are plant-derived alkaloids, which offer symptomatic relief from AD [8] . In addition to these approved natural products, many potent cholinesterase inhibitors have been reported in literature [7] . Some reported potent cholinesterase inhibitors are nevertheless presented in Figure 1 .

Our study is an attempt to identify and compare potential candidates from the following eight selected plant species (family affiliations of all species in Table 1 ) Blumea lacera (Burm.f.) DC., Byttneria pilosa Roxb., Clerodendrum infortunatum L., Cyclea barbata Miers, Mikania micrantha Kunth, Smilax guianensis Vitman, Spermacoce articularis L.f., and unbergia grandiflora Roxb. for anticholinesterase activity in rejuvenating and improving the memory and cognitive function. A study of ethnopharmacological background of the selected species is presented in Table 1 .

2.1. Chemicals. Acetylcholinesterase (AChE) from electric eel (Electrophorus electricus) (type VI-s, lyophilized powder), acetylthiocholine iodide (ATCI), butyrylcholinesterase (BChE) from equine serum (lyophilized powder), butyrylthiocholine iodide (BTCI), and 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Trizma hydrochloride (Tris-HCl) and bovine serum albumin (BSA) were obtained from Sigma-Aldrich (Steinheim, Germany). Deionized water was produced using a Milli-Q water purification system (Millipore, Bedford, MA, USA). Figure S1 ).

Freshly collected plant leaves were dried in the shade and then pulverized. To prepare the extract, 10 g of each plant part was extracted in 50 mL solvent employing the cold extraction method as described in Table 1 , at room temperature. Solvent polarity has been selected on the nature of phytochemicals present in the plant species [17] . Filtered extracts were dried in a rotary evaporator under reduced pressure at 35°C temperature.

Activities. ChE inhibitory activity was measured based on Ellman's method [18] as reported in a previous study [19] . In the inhibition of AChE activity, the enzyme hydrolyzes the substrate acetylthiocholine resulting in the product thiocholine, which reacts with Ellman's reagent (DTNB) to produce 2-nitrobenzoate-5-mercapto-thiocholine and 5thio-2-nitrobenzoate, detectable at 405 nm. In this assay, 25 μL of acetylthiocholine iodide (5 mM), 125 μL of DTNB (3 mM), 50 μL of buffer B (50 mM Tris-HCl, pH 8 containing 0.1% BSA), and 25 μL of each test extract solution at the different concentrations or negative control (25% DMSO in MeOH) were mixed and incubated for 10 min at 37°C. e reaction was started by adding 25 μL of 0.05 U/mL AChE. e absorbance was measured at 405 nm, and the reaction rates were calculated by MARS Data analysis software (SPECTROStar NANO, BMG Labtech, Germany). Estimation of BChE inhibition was performed in a similar way described above using 25 μL of 5 mM butyrylthiocholine iodide as substrate and 0.05 U/mL of BChE as an enzyme. Galantamine was used as a positive control for both enzymes. ree independent assays were performed in triplicate at different concentrations.

To investigate the type of inhibition of the effective extracts, the enzyme activity was determined in the presence of increasing concentrations of substrate ATCI (0.16-20 mM) and the absence or presence of two/three concentrations of each extract. e analysis of the type of inhibition of AChE activity was determined by the Lineweaver-Burk (LB) plot, whereas the kinetic parameters km and Vmax were obtained by curve fitting according to the classical Michaelis-Menten equation by using GraphPad Prism version 6.

2.6. Molecular Docking Protocol. e crystal structure of cholinesterase enzymes has been collected from RCSB PDB (rcsb.org) [20] . PDB ID 4EY7 [21] and 4AQD [22] were assigned for AChE and BChE, respectively. e proteins were prepared in Swiss PDB Viewer [23] and PyMOL.

Nine compounds ( Figure 2 ) from Blumea lacera [24] [25] [26] [27] [28] [29] were selected for molecular docking study considering their chemical nature and action on cholinesterase [30, 31] . e structures were drawn in ChemDraw and Chem3D. e geometry optimization of all structures was carried out by the semiempirical PM6 method in Gaussian 09 [32] . e optimized structures were collected in PDB format suitable for molecular docking. e molecular docking of all compounds against AChE and BChE was performed in PyRx Virtual Screening Tool (Version 0.8) [33] . e compounds were saved in AutoDock ligand format (PDBQT) before docking. e grid box center and dimensions were set accordingly to cover the substrate-binding sites of the protein.

For AChE, the grid box center was reserved at X � −2.0303, Y � 38.0503, and Z � 29.9752, and the dimension (angstrom) was X � 42.2467, Y � 50.5623, and Z � 41.3235. In case of BChE, the grid box center was fixed at X � 1.6818, Y � −1.3768, and Z � −10.0914 and the dimension (angstrom) was X � 39.1883, Y � 39.4042, and Z � 33.0087. e docking process was performed in triplicate for every ligand and the average of the binding affinities has been presented. Furthermore, the extracted ligand from the crystal structure was docked with the optimized protein following the same protocol, which validated our docking protocol [34] . e docked ligand-protein complexes were visualized in BIO-VIA Discovery Studio (Version 4.5) to detect the noncovalent interactions.

Analysis. Data are given as the mean ± SD (n � 3). IC 50 values were calculated via nonlinear regression analysis using GraphPad Prism v. 6.0 (GraphPad Software Inc., USA). One-way analysis of variance (ANOVA) and Tukey test were used to compare means among extracts; an observation was considered statistically significant if the p value is less than 0.05 (p < 0.05).

e extracts of eight plant species used as herbal medicine in different disorders were tested for AChE and BChE inhibitory activity using Ellman's method. e results are shown in Table 2 , representing the IC 50 values for the eight extracts prepared. e lowest IC 50 AChE inhibitory activity was found for Blumea lacera followed by Cyclea barbata, Smilax guianensis, Byttneria pilosa, and unbergia grandiflora displayed values of 150 ± 11, 176 ± 14, 205 ± 30, 221 ± 2, and 252 ± 22 µg/mL, respectively. Figure (Table 2) . e IC 50 value of reference standard galantamine for BChE assay was 5.97 ± 0.97 µg/mL (16.2 ± 2.6 µM). e inhibition of BChE is also increasing corresponding to the concentration of extracts ( Figure 4) . Our results demonstrated a dual activity of 

(a) (b) (C) (d) (e) (f) (i) (g) (h) CH 3 CH 3 CH 3 CH 3 H 3 C O O O O O O O OH HO CH 3 COOCH 3 COOCH 3 CH 3 CH 3 CH 3 H 3 C H 3 C O

Blumea lacera, Cyclea barbata, and Byttneria pilosa extracts. Since AChE is mainly located in the central nervous system (CNS) and BChE is more abundant in the peripheral system, the potency toward both ChE found in the extracts is of paramount importance. ese findings could activate mostly the central as well as peripheral cholinergic transmission to improve the mental abilities of AD patients. Among the eight screened extracts, Blumea lacera, Cyclea barbata, Smilax guianensis, and Byttneria pilosa were selected as efficacious candidates as sources of strong ChE (AChE and BChE) inhibitors. Due to the multifactorial pathogenesis of AD, multitargeted drugs will be preferred as the effective therapeutic strategy against AD. Blumea lacera, a widely distributed herb in Bangladesh, is locally known as Kukursunga or Shimura. B. lacera is used to cure spasms, fever, and bronchitis and alleviate burning sensation [9] . Leaves of the plant are astringent, stimulant, anthelmintic, antiscorbutic, and diuretic [9, 27] and they are also used as antidysenteric and anti-inflammatory remedies [9] . Acetone extract of leaves of B. lacera showed an IC 50 value of 150 ± 11 and 297 ± 13 μg/mL for AChE and BChE inhibition, respectively; efficacy of these leaves on enhancing cognitive functions might be due to the presence of terpenoids [35] . Acetone-water (70 : 30) extract of B. lacera displayed lower activity (IC 50 value of 197 and 1040 µg/mL for AChE and BChE inhibition, respectively) compared with acetone extract.

Cyclea barbata is a tropical indigenous plant widely distributed in Asia, especially in Bangladesh. is climber is traditionally used against various diseases such as headache, epilepsy, allergy, asthma, lipoma, tetanus, and throat sore [9] . Acetone-water (70 : 30) extract of Cyclea barbata exhibited IC 50 value of 176 ± 14 and 124 ± 2 µg/mL for AChE and BChE inhibition, respectively, which might be owing to its high alkaloid contents [36] .

Smilax guianensis, locally known as Kumarika, is believed to possess CNS-modulating properties and is used in many herbal formulations. S. guianensis is also used in the treatment of epilepsy, venereal and skin diseases, fever, swellings, sores, and abscesses [9, 37] . Young stem is used to improve memory loss. e root is used for the management of rheumatism and pain in the lower extremities [15, 38] . Dried leaves of S. guianensis methanolic extract inhibited AChE with an IC 50 value of 205 ± 31 μg/mL which might be due to the presence of steroidal saponin glycosides [39] , while no significant inhibition on BChE was observed.

Byttneria pilosa, a large woody climber, is widely distributed in Chittagong, Bangladesh. Leaves and stems of this species are usually used to treat snakebite by the traditional healers and indigenous people in Chittagong hill tracts, Bangladesh. It is also used to cure scabies, bone fracture, and elephantiasis [9, 12, 13] . Methanolic extract of B. pilosa leaves revealed its AChE and BChE inhibitory potentials with an IC 50 value of 221 ± 2 and 536 ± 23 μg/mL, respectively, because of the presence of favorable anticholinesterase natural products such as alkaloid, saponin, and terpenoid [13] .

Both acetylcholinesterase and butyrylcholinesterase are the key enzymes in the cholinergic nervous system. erapies designed to reverse the cholinergic deficit in AD are mostly based on inhibitors of AChE. Several studies revealed that cholinesterase inhibitors could act on multiple therapeutic targets such as preventing the formation of β-amyloid plaques, antioxidant activity, and modulation of APP processing [40] . Moreover, AChE inhibition is also considered a promising therapeutic strategy for other types of dementia, myasthenia gravis, glaucoma, and Parkinson's disease [41] . Nonetheless, there is still a need to explore the nature of newer potent and long-lasting ChE inhibitors with minimal side effects. Natural products have already been proven to be promising sources of useful ChE inhibitors [7, 42] . Bioassayguided approaches have studied many plants for the search of new AChE inhibitors with lower toxicity and higher CNS penetration. e kinetic study revealed the potential mechanism of enzyme inhibition. e relationship between substrate concentration and reaction velocity was in good agreement with Michaelis-Menten kinetics. In absence of inhibitors, the km value of the substrate ATCI was 10.4 ± 1.8 mM, and the Vmax was 12.4 ± 1.1 mM/min for the electric eel acetylcholinesterase. Galantamine was used as positive control showing a competitive inhibition. e extracts Blumea lacera, Byttneria pilosa, and Smilax guianensis showed uncompetitive inhibition in which inhibitor only binds to enzyme-substrate complex (Table 3 ). Figure 5 represents the enzyme kinetics of Blumea lacera. In the uncompetitive pattern, both Vmax and km decrease, and the kinetic pattern produced parallel lines with increasing extract concentration. e mode of inhibition of Cyclea barbata seemed to be ambiguous at lower concentration while Vmax and km both increased, whereas at higher concentration, it reflected competitive inhibition (km increased and Vmax remained unchanged) ( Figure 6 ). ese types of inhibition could be due to the presence of various phytochemicals typical in medicinal plants. e kinetic study against BChE demonstrated an ambiguous inhibition mode.

In silico molecular docking is an efficient tool in recent structure-based drug design. In this anticholinesterase screening study, Blumea lacera exhibited the highest inhibition among the eight plant species. Here, the docking study was conducted to identify the responsible compounds from Blumea lacera on anticholinesterase potential. However, the binding affinity and binding pose of selected compounds (Figure 2 ) with the AChE and BChE could be considered as proof of our claimed biological outcome. All the ligands showed good binding affinity where some of them interacted with the catalytic site of proteins (Table 4) . BL1, BL2, and BL7 demonstrated the highest binding affinity (kcal/mol) with AChE, whereas BL5, BL6, and BL8 reflected the highest binding affinity with BChE.

Ligands' interaction with the catalytic triad residues of acetylcholinesterase is essential to inhibit enzymatic activity. We analyzed all the ligands binding poses to investigate the interaction of the ligands with the AChE. e majority of the ligands did not interact with the catalytic triad residues of AChE that validated the uncompetitive inhibition mode in the experimental result ( Figure 5 ; Supplementary Material, Figure S2 ) of Blumea lacera. ree out of nine compounds Figure S3 ). e active site of both AChE and BChE consists of two subsites [43, 44] . e esteratic site (also called catalytic triad) of human AChE consists of three essential amino acids Ser203, Glu334 and, His447, while the PAS is composed of five residues Tyr72, Asp74, Tyr124, Trp286, and Tyr341. e catalytic triad of human BChE is comprised of Ser198, His438, and Glu325, whereas the PAS contains Asp70, Phe329, Trp82, Tyr128, and Tyr332 [45] [46] [47] [48] . e binding of a ligand highly regulates the cholinesterase enzyme inhibition at the catalytic site. e aromatic ring in the ligand forms a few pi-alkyl, pi-pi, pi-pi T-shaped interactions with the amino acid residues of the protein.

e 5,4′-dihydroxy-6,7,3′-trimethoxyflavone (BL1), 5-hydroxy-3,6,7,3′,4′-pentamethoxyflavone (BL4), and campesterol (BL7) interact Evidence-Based Complementary and Alternative Medicine with the catalytic residues Ser203 and His447 of AChE reflecting the competitive inhibition ( Figure 7 ). BL1 and BL4 interact with most of the peripheral site residues of AChE, while BL7 interacts only with Tyr124 and Tyr341. Despite having a high binding affinity, 19α-hydroxyurs-12ene-24,28-dioate 3-O-β-D-xylopyranoside (BL2) does not interact with the PAS and catalytic triad residues. BL1, BL4, and BL7 displayed 16, 16, and 12 hydrophobic interactions as well as three, ten, and one hydrogen bonds with AChE, respectively (Table S1 ). Many agents could prevent the formation of amyloidogenic protein through blocking the peripheral binding site of AChE and offers additional therapeutic benefits besides the inhibitory activity [49] . BL1 and BL4 acted as a dual-binding agent, blocking both the peripheral and the catalytic binding sites of AChE simultaneously, and could offer additional benefits in AD management. All compounds, except BL9, exhibited a hydrogen bond with the catalytic site of BChE. e hydroxyl groups in the ligands' side chain have a crucial role in ligandprotein interactions by forming a hydrogen bond with the protein residues. Kaempferol 3-O-α-L-rhamnopyranosyl-(1⟶6)-β-D-glucopyranoside (BL5) formed a hydrogen bond with Ser198 and His438 with side-chain oxygen of BChE. Kaempferol 3-O-(2″,6″-di-O-α-L-rhamnopyranosyl)-β-Dglucopyranoside (BL6) interacted with the catalytic residues and PAS residues with side-chain oxygen and hydrogen atoms, forming hydrogen bonds with Asp70, Tyr332, Ser198, and His438 (mixed inhibition). BL8 forms hydrogen bonds with Ala277, Asn289, Ser287, Ala328, and r284. BL5, BL6, and BL8 formed 9, 7, and 6 hydrogen bonds with BChE, respectively (Table S2 ). e methyl groups in the side chain of the ligands play a pivotal role in developing pi-alkyl interaction with the amino residues of the protein.

Moreover, BChE has the ability to accommodate bulkier compounds compared to AChE because the active site of BChE has many aromatic residues replaced by residues with aliphatic side chains, such as leucine (Leu) and valine (Val) [49] . Due to space availability, BL5, BL6, and BL8 were able to accommodate and docked completely into the base of the active site and held in place by the hydrogen bond with Ser198 and His438. BL9 might not reach the peripheral site or the catalytic site due to its large molecular structure.

e chemical structure of natural compounds reflects their binding affinity as well as biological activity. It is noted that hydroxylation improves the inhibitory activities of flavonoids against ChE, while methoxylation may decrease or increase these activities [50] . In docking study, it has been observed that the methoxylation of flavonols decreased the binding affinity for ChE; on the other hand, glucose substitution decreased the binding affinity toward AChE but enhanced the affinity for BChE. In case of triterpenoids, glucosylation attenuated the binding affinity for AChE, while binding affinity has been increased against BChE.

e various traditionally used plant extracts were screened for cholinesterase (AChE and BChE) inhibition assay. e currently approved therapies for AD are based on improving cholinergic transmission via the inhibition of ChE which only provide fair improvement in memory and cognitive functions. is study shows that, out of eight tested species, the leaves of Blumea lacera, Cyclea barbata, Smilax guianensis, and Byttneria pilosa can be selected as promising sources of effective ChE inhibitors. e cholinesterase inhibitory potential of Blumea lacera has also been established by molecular docking studies. Since Blumea lacera and Cyclea barbata extracts can act on both in vitro AChE and BChE at a bit higher dose compared to galantamine, further evaluation is required to identify the active ingredients and assess their safety and bioavailability in animal models. With AD being a multifaceted neurodegenerative disease, other targets must be considered for future investigations on these extracts or single compounds along with pharmacokinetic, toxicity, and compound stability studies to establish doseactivity/toxicity relationship and any side effects for potential clinical application.

Alzheimer's disease AChE: Acetylcholinesterase APP: Amyloid precursor protein ATCI: Acetylthiocholine iodide BChE: Butyrylcholinesterase BSA:

Bovine serum albumin BTCI: Butyrylthiocholine iodide CNS: Central nervous system DTNB: 5, 5′-Dithio-bis-(2-nitrobenzoic acid).

e data used to support the findings of this study are available from the corresponding author upon request.

e authors declare that they have no conflicts of interest.

Christian Zidorn and Md Josim Uddin acknowledge support from the German Academic Exchange Service (DAAD-91649878). e authors are grateful to A. S. M. Ali Reza from International Islamic University Chittagong for his support in plant collection.

Figure S1-1 shows details of collection and identification of Blumea lacera (Burm.f.) DC.; Figure S1 -2 shows details of collection and identification of Byttneria pilosa Roxb.; Figure  S1 -3 shows details of collection and identification of Clerodendrum infortunatum L.; Figure S1 -4 shows details of collection and identification of Cyclea barbata Miers; Figure  S1 -5 shows details of collection and identification of Mikania micrantha Kunth; Figure S1 -6 shows details of collection and identification of Smilax guianensis Vitman; Figure S1 -7 shows details of collection and identification of Spermacoce articularis L.f.; Figure S1 -8 shows details of collection and identification of unbergia grandiflora Roxb.; Figure S2 -1 shows the best rank pose (2D and 3D) of noncovalent interactions between ligand and AChE; Figure  S2 -2 shows the best rank pose (2D and 3D) of noncovalent interactions between ligand and AChE; Figure S3 -1 shows the best rank pose (2D and 3D) of noncovalent interactions between ligand and BChE; Figure S3 -2 shows the best rank pose (2D and 3D) of noncovalent interactions between ligand and BChE; Table S1 shows noncovalent interactions of BL-1, BL-4, and BL-7 with AChE; Table S2 shows noncovalent interactions of BL-5, BL-6, and BL-8 with BChE. (Supplementary Materials) 

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