key: cord-0019324-0pqo9c6u
authors: Śladowska-Kozłowska, Joanna; Litwin, Mieczysław; Niemirska, Anna; Wierzbicka, Aldona; Wawer, Zbigniew T.; Janas, Roman
title: Change in left ventricular geometry during antihypertensive treatment in children with primary hypertension
date: 2011-12-01
journal: Pediatr Nephrol
DOI: 10.1007/s00467-011-1916-8
sha: 342ca8cb97b428f8adfb1aa3153b1255038a635d
doc_id: 19324
cord_uid: 0pqo9c6u

The pattern of the left ventricle (LV) has important significance in adults with hypertension. The aim of the present study was to analyze changes and determinants of LV geometry after 1 year of antihypertensive treatment in children with primary hypertension (PH) in relation to metabolic abnormalities and anthropometrical parameters. In 86 children (14.1 ± 2.4 years) with newly diagnosed PH, LV geometry and biochemical parameters before and after 12 months of standard antihypertensive therapy were assessed. At baseline, normal LV geometry (NG) was found in 42 (48.9%), concentric remodeling (CR) in 4 (4.6%), concentric hypertrophy (CH) in 8 (9.3%), and eccentric hypertrophy (EH) in 32 (37.2%) patients. The prevalence of NG in patients with severe hypertension was significantly lower than in patients with ambulatory hypertension. There were no differences in dipping status in relation to LV geometry. Patients with CH and EH were more viscerally obese than patients with NG. Patients with CH had higher diastolic blood pressure in comparison with EH patients (p < 0.05). The main predictor of relative wall thickness (RWT) was the triglycerides to high density lipoprotein cholesterol (TG/HDL) ratio (R(2) = 0.319, β = 0.246, p = 0.004). Patients received 12 months of antihypertensive treatment, either lifestyle modification only (n = 37) or lifestyle modification plus antihypertensive medications (n = 49) if severe ambulatory hypertension or target organ damage were present. After 12 months of treatment the prevalence of EH (37.2% vs 18.6%, p = 0.003) decreased but prevalence of CH did not change. Patients in whom RWT decreased also decreased waist circumference and TG/HDL; the main predictor of RWT decrease was a decrease of the TG/HDL ratio (β = 0.496, R(2) = 0.329, p = 0.002). In adolescents with PH, LV geometry is related to central obesity and insulin resistance. Decrease of abdominal obesity and insulin resistance are the most important predictors of normalization of LV geometry, however CH has lower potential to normalize LV geometry. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00467-011-1916-8) contains supplementary material that is available to authorized users

Subclinical target organ damage (TOD) is present in 30-40% of children with primary hypertension (PH) at the diagnosis of elevated blood pressure (BP) [1] [2] [3] [4] [5] and correlates with metabolic abnormalities, central obesity, and prevalence of metabolic syndrome (MS) [6] [7] [8] . This has clinical importance because of increased cardiovascular morbidity in adults with TOD [9, 10] . Left ventricular hypertrophy (LVH) is the most clinically useful marker of TOD. The left ventricle (LV) may adapt to hypertension by developing concentric or eccentric LVH (CH, EH respectively), concentric left ventricular remodeling (CR), or by retaining normal LV geometry (NG). Each geometric pattern is associated with a distinct combination of pressure and volume stimuli, contractile and diastolic efficiency (reduced in those with CR or CH) and prognosis (worst with CH and best with NG) [11, 12] . The relation between LV remodeling and metabolic abnormalities in children with PH has not been fully explored [13] .

To date, there are only limited data on the effectiveness of antihypertensive treatment in adolescents with PH and even fewer data exist on the regression of LVH and changes in geometric abnormalities [14] [15] [16] . Previously, we found that the main determinant of LVH regression was a decrease in visceral obesity [16] . The aim of the present study was to analyze changes and determinants of LV geometry after 1 year of antihypertensive treatment in the same group of children with PH in relation to metabolic abnormalities and anthropometrical parameters.

The study adhered to the principles of the Declaration of Helsinki and was approved by the local Ethics Committee. All parents and children who were literate and able to understand age-based patient information provided written informed consent.

The study group has already been described [16] . In short, 86 children (20 girls) with untreated PH, mean age 14.1 (range: 5-17 years) and who completed all investigative procedures, were enrolled onto the study. PH was diagnosed after a thorough clinical and laboratory diagnostic work-up, according to recently published recommendations [17, 18] . The exclusion criteria were: diagnosis of secondary hypertension, previous use of antihypertensive drugs, presence of any significant chronic disease (except for PH), any acute disease, including infections, in the 6 weeks preceding enrollment, and incomplete data.

Normal office blood pressure values were taken from the Updated 4th Task Force Report [17] . In all patients the diagnosis was confirmed by 24-h ambulatory blood pressure monitoring (ABPM). ABPM was performed at the initial visit and after 12 months. Recordings lasting at least 20 h with at least 80% of records considered valid.

Blood pressure values were calculated from the ABPM data as the mean 24-h systolic (SBP) and diastolic (DBP) blood pressure and presented as absolute values and as systolic and diastolic blood pressure indices (SBPI and DBPI respectively) calculated as the ratio of SBP or DBP to the 95th percentile for age and gender based on the pediatric ABPM reference data [19] . We used a recently published classification system based on ABPM to classify patients as having normal blood pressure, ambulatory hypertension, or severe ambulatory hypertension [20] .

All patients underwent the following assessments: anthropometrical measurements including body mass index (BMI), waist circumference (WC), waist-to-hip ratio (WHR) and waist-to-height ratio (WHTR), echocardiography, urinary albumin excretion, serum glucose and insulin, blood lipids, serum homocysteine, serum uric acid and C-reactive protein (hsCRP), and serum asymmetric dimethylarginine (ADMA) concentrations. In addition, in all patients oxidative stress markers, i.e., thiobarbituric acid reactive substances (TBARS), oxidized LDL-cholesterol (oxyLDL) concentrations, and anti-oxidative defense (reduced glutathione concentration [GSH], glutathione peroxidase activity [GPX]) were measured. Obesity and overweight was diagnosed according to International Obesity Task Force recommendations [21] . Because some of our patients were below 10 years of age, metabolic syndrome was diagnosed as described previously, and when at least three criteria were present (BMI ≥ 95th percentile for age and gender, arterial hypertension, serum triglycerides>110 mg/dl, fasting plasma glucose>100 mg/dl or>140 mg/dl at 2 h of oral glucose tolerance test and HDL cholesterol<40 mg/dl) [6] .

All echocardiography (ECHO) examinations were performed by one examiner who knew the clinical diagnosis, but was not aware of the severity of PH or effectiveness of treatment. ECHO measurements were performed according to the American Society of Echocardiography guidelines [22, 23] . To standardize left ventricular mass to height, the left ventricular mass index (LVMi) was calculated according to the deSimone formula [24] . LVH was defined as an LVMi value above 95th percentile for age and gender based on the pediatric LVMi reference data of Khoury's [25] . Relative wall thickness (RWT) was measured at end diastole as the ratio of posterior wall thickness plus interventricular septum thickness over LV internal dimension. A RWT of 0.41, which represents the 95th percentile for RWT for normal children and adolescents, and the 95th percentile of LVMi for age and gender for normal children and adolescents were used as cut-off points in the evaluation of LV geometry [26] . Normal geometry was defined as RWT <0.41 and LVMi< value of 95th percentile, RWT <0.41 and LVMi>value of 95th percentile of LVMi defined EH, RWT >0.41 and LVMi> value of 95th percentile of LVMi defined CH, and RWT >0.41 and LVMi<95th percentile of LVMi defined CR.

Blood samples were taken after 12 h of fasting and were immediately sent to the laboratory. Plasma glucose levels were measured using a Dimension analyzer. Plasma insulin concentrations and glycated hemoglobin (HbA1C) were measured by radioimmunoassay. Oral glucose tolerance test was carried out in all patients after oral ingestion of 1 g/kg body weight (maximum 75 g) of glucose. Insulin resistance was expressed as homeostasis model assessment for insulin resistance (HOMA-IR) and as a triglycerides to high density lipoprotein cholesterol (TG/HDL) ratio [27, 28] . The hsCRP concentration was assayed using a highlysensitive immunoturbidimetry kit (Orion Diagnostica). Plasma lipid peroxides were determined with the spectrofluorometric method of Yagi and expressed as thiobarbiturate reactive substance (nmol/ml). Glutathione and glutathione peroxidase activity were used as indicators of antioxidant status and were measured spectrophotometrically in erythrocytes (GSH-420(Oxis) and GPx-340(Oxis)). OxyLDL and asymmetric dimethyloarginine (ADMA) concentrations were measured by ELISA using a commercially available kit. Urinary albumin excretion in 24-h samples was determined by the immunonephelometric method. The laboratory procedures have been described in detail in a previous study [6] .

The principles of treatment were the same as previously described [16] . In short, pharmacological treatment was started in patients who had significant TOD and/or severe ambulatory hypertension. BP was checked at 3 and 6 months during ambulatory visits. If after 3 or 6 months office BP was still within the hypertensive range, ABPM was carried out. In patients who still had ambulatory or severe ambulatory hypertension, pharmacotherapy was started or another drug was given. Finally, in all patients ABPM was done after 12 months of treatment. Patients who after 12 months of treatment lowered ABPM to below the 95th percentile were treated as responders and those who did not, as non-responders. Drug therapy was based on an angiotensin converting enzyme inhibitor (ACEi), enalapril (0.2-0.3 mg/kg/day bid). Patients who had asthma or did not tolerate ACEi were prescribed an angiotensin 2 receptor type 1 blocker (ARB), losartan (0.7-1 mg/kg/day in one or two daily doses). If patients were unable to buy the prescribed medications (enalapril or losartan), amlodipine was used at a standard dose of 5 mg once daily. Detailed descriptions of the principles of treatment are shown in the Electronic Supplementary Material (ESM).

Because the analyzed groups included subjects of different ages and genders, BMI and WC values were expressed both as absolute values and as SDS for age and gender. LVM values in grams were standardized to height in meters 2.7 . The change of measured parameters was expressed as a delta value (Δ), i.e., the difference between measurement at 12 months and at the start of treatment. Homogeneity of variance was checked with the Levene test. Continuous variables with a normal distribution were compared using Student's t test for independent variables. Continuous values with non-normal distribution were compared using the Mann-Whitney U test. Comparison between groups was evaluated with ANOVA test using Bonferroni correction. Measurements taken at the start and after 12 months of treatment were compared using the Wilcoxon test. Dichotomous variables were compared using the Chi-squared test and change in prevalence during treatment was analyzed with the McNemar test. Correlation analysis was carried out with the Spearman test. RWT and Δ for RWT were dependent variables. Variables with significant correlation or that differed between groups of patients in whom LV geometry was normalized or not, were then included in the step-wise multiple regression analysis. A p value less than 0.05 was regarded as statistically significant. A p value ranging between 0.05 and 0.1 was regarded as a statistical tendency.

Descriptive demographic, clinical, and laboratory data obtained at the start and after 12 months of treatment are summarized in Table S1 (ESM).

According to the International Obesity Task Force criteria, 67% of patients were obese or overweight (58 patients). Fortytwo (48.9%) patients had NG, 4 (4.6%) had CR, 8 (9.3%) had CH, and 32 (37.2%) EH. Patients with CH and EH were more viscerally obese than patients with NG (Table 1) . Patients with CH in comparison with patients with EH had greater DBP and a tendency towards a greater birth weight (p=0.1). EH was more prevalent among obese than non-obese patients. Patients with severe ambulatory hypertension (n=36) had greater LVMi than patients with ambulatory hypertension (n=50; 42.3 ±12.1 g/m 2.7 vs 35.7 ±8.7 g/m 2.7 , p<0.01) and there was a tendency towards a higher prevalence of LVH (58% vs 38%, Chi-squared=3.48, p=0.06). The prevalence of NG in patients with severe ambulatory hypertension was significantly lower (13 patients, 36.1% vs 29 patients, 58%, p=0.04, Chi-squared=4.01). Patients with ambulatory and severe ambulatory hypertension did not differ in terms of RWT. There were no differences regarding dipping profiles among patients with different patterns of LV geometry. RWT correlated with WHR (p< 0.05, r = 0.25), TG/HDL ratio (p<0.05, r=0.29), and TBARS concentration (p<0.01, r=0.35). The main predictor of RWT was the TG/HDL ratio (R 2 =0.319, β= 0.246, p=0.0004), and the main predictor of LVMi was WC (R 2 =0.279, β=0.561, p=0.008).

Throughout the study, 37 (43%) patients were prescribed only non-pharmacological therapy, including dietary advice and increased physical activity. Thirty-four (39.5%) patients received one antihypertensive medication, 9 patients (10.5%) received two drugs (ACEi or ARB plus amlodipine), and 6 (7%) received three drugs (ACEi or ARB plus amlodipine and metoprolol). As reported earlier, overall after 12 months, BP normalized below the 90th percentile in 54 (62.8%) patients, 10 patients were in the pre-hypertensive range (11.6%), 21 (24.4%) patients had ambulatory hypertension, and 1 (1.2%) had severe ambulatory hypertension, but the decrease in hypertension severity was significant [16] . Table 2 ). The decrease in DBP, DBPI, and TG/HDL ratio after 12 months in patients with CH was significantly lower than in patients with EH and in patients with NG. Also, the decrease in uric acid concentrations in patients with CH was lower than in patients with EH (Table 3 ). In general, patients who had lowered RWT decreased WC and the TG/HDL ratio to a significantly greater extent than patients with an increase or stabilization of RWT (Table 4 ). The prevalence of metabolic syndrome decreased in the subgroup of patients who had decreased RWT in comparison with the subgroup of patients with progression/stabilization of RWT. Similarly, the prevalence of metabolic syndrome decreased (8 vs 1 case) only in the group of 58 patients who had decreased LVMi (p=0.02; Chi-squared=5.14). On the contrary, the prevalence of metabolic syndrome did not change among patients who did not have a decrease in LVMi. The decrease in RWT correlated with decreases in WC (r=0.25, p<0.05), TG/ HDL ratio (r=0.38, p<0.01), albuminuria (r=0.32, p<0.05), as well as with values of the TG/HDL ratio (r=−0.26, p< 0.01), RWT (r=−0.64, p<0.001), and LVMi (r=−0.35, p< 0.001) at the first evaluation and thiobarbiturate reactive substance concentration at the first and the second evaluation (r=−0.22, p<0.05 and r=−0.21, p<0.05 respectively). The main predictor of RWT decrease was a decrease in the TG/HDL ratio (β=0.496, R 2 =0.329, p=0.002).

There are three main findings of our study. First, EH is the most frequently found pattern of LV remodeling in NG2, normal geometry after 12 months of antihypertensive treatment; CR2, concentric remodeling after 12 months of antihypertensive treatment; CH2, concentric hypertrophy after 12 months of antihypertensive treatment; EH2, eccentric hypertrophy after 12 months of antihypertensive treatment; BMI, body mass index; WC, waist circumference; WHR, waist to hip ratio; WHtR, waist to high ratio; SBP/24h, mean systolic blood pressure in 24-h ambulatory blood pressure monitoring; SBPI/24h, index of mean systolic blood pressure in 24-h ambulatory blood pressure monitoring; DBP/24h, mean diastolic systolic blood pressure in 24-h ambulatory blood pressure monitoring; DBPI/24h, index of mean diastolic systolic blood pressure in 24-h ambulatory blood pressure monitoring; TG/HDL, triglycerides to high-density lipoprotein cholesterol ratio; HOMA-IR, homeostasis model assessment for insulin resistance; ADMA, serum asymmetric dimethyloarginine; SDS, standard deviation score adolescents with PH. Second, the main determinants of pathological remodeling of the LV are visceral obesity and metabolic abnormalities. Third, both LVH and EH can be reversed and LV geometry can fully normalize; however, CH has less potential to normalize LV geometry. Daniels et al. already found that EH was the most frequent pathological pattern of LV remodeling [1] . This type of LV remodeling is typical for states of volume overload or as a consequence of long-standing cardiac disease and heart failure [10, 11] . However, EH is also typical for obesity, and obesity is the dominant intermediate phenotype of children with PH [6, 29] . EH was also found to be the most prevalent form of LVH in other studies in hypertensive adolescents and young adults with relatively short durations of PH. In the Strong Heart Study, in which as many as 65% of patients had a BMI above 30, EH was present in 20% of patients aged 14-39 with PH, and CH occurred in just a few of them [30] . Assessing both the pharmacologically treated and untreated children as well as children with PH and secondary hypertension, Hanevold et al. found CH in as many as 16% of patients, while EH occurred in 20% of children [3] . In contrast to our findings, Richey et al., who studied LV geometry in a group of children with PH at the time of diagnosis and before the initiation of antihypertensive therapy, found CH and EH with the same frequency (19.1%), and NG in 51.5% of patients. They also observed that patients with EH had a higher DBP compared with other groups [31] . The differences between our results and those of Richey et al. may be explained by racial differences. All our patients were of Caucasian origin, whereas 75% of the patients evaluated by Richey et al. were of African-American origin. We found higher DBP in patients with CH than in patients with EH, both at diagnosis and after 1 year of treatment. It corresponds with findings in the Bogalusa Heart Study that DBP in childhood-predicted CH in adulthood [13] . This study also confirmed the correlation between CH and DBP and with impaired glucose tolerance.

There are no pediatric data concerning the risk associated with the type of LV remodeling. Nonetheless, Border et al. found diastolic dysfunction in as many as 36% of children with PH, and it mostly affected children with CH [32] . Because LV remodeling depends on many factors, including duration of hypertension, CH may also develop in obese patients [33, 34] . This was shown by Daniels et al., who reported that duration of hypertension was significantly longer in children with CH in comparison with patients with other patterns of LV remodeling [1] .

Similar to other studies [35] , we did not find any strong relation between BP and LVMi. The relatively small impact of BP on LV may be due to the relatively low absolute values of blood pressure. As discussed previously, although blood pressure values in children with PH are within the hypertensive range in relation to age and growth, they are relatively low in comparison with those found in secondary hypertension or in hypertensive adults [16] . Second, the range of absolute values of blood pressure observed in our study was relatively narrow.

The TG/HDL ratio is a marker of metabolic abnormalities typical for visceral obesity and hyperinsulinemia. These abnormalities are also typical for adolescents with PH. Hypertriglyceridemia with low HDL-cholesterol concentrations indirectly points to insulin resistance [27] . However, insulin resistance is linked to exposure to other growth factors causing LVH [36] . We found that the main determinants and correlates of RWT are the TG/HDL ratio, WC, and oxidative stress. Although we did not find any relation between insulin or HOMA-IR and RWT, patients whose LV geometry did not normalize or who developed CH had greater HbA1C and insulin concentrations during the oral glucose tolerance test. Moreover, they increased insulin secretion after the glucose load.

The third finding of our study is that in children with PH, LVMi and LV geometry can normalize. Although there is a small amount of data on the changes in LVMi during treatment in children with PH, we did not find any data on the changes in LV geometry. Although the prevalence of NG increased and that of EH decreased significantly, the prevalence of CH did not change. Moreover, patients with CH did not experience lower BP to the same extent as patients with EH. This suggests that CH might be more difficult to reverse. One may hypothesize that CH is a marker of advanced TOD, also causing resistance to antihypertensive treatment. This suggests that the assessment of LV geometry might be valuable for the evaluation of further risk and may be an indication for more intensive treatment.

One of the main limitations of our study is the relatively low number of patients. The low absolute number of patients with CH and CR limits the possibility of statistical analysis. Second, because our patients were treated both pharmacologically and with non-pharmacological measures, it is difficult to evaluate the effects of specific treatments. Moreover, we did not monitor adherence to non-pharmacological treatment including sodium intake and physical activity. Similarly, although we treated patients according to guidelines, we used different drugs (ACEi or ARBs). Those limitations are why we could only evaluate determinants of change in LV geometry, but not the effects of specific drugs. Another limitation is the racial and ethnic homogeneity of our patients. For this reason our results cannot be extrapolated to other racial groups. Gender distribution was similar to that of other studies and is typical of PH in adolescence [1, 31] . However, potential gender-related effects on the evolution of LV geometry should be observed in a larger group of patients.

Concluding, our results indicate that in Caucasian children with PH, the most prevalent type of LVH is EH. The main determinants of EH are visceral obesity, of CH-insulin resistance. Antihypertensive treatment leads to significant improvement and normalization of LV geometry. However, patients with CH are less prone to normalized LV geometry and may require more intensive treatment.

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