key: cord-0022975-yh0103ws authors: Wines, Bruce D.; Trist, Halina M.; Esparon, Sandra; Impey, Rachael E.; Mackay, Graham A.; Andrews, Robert K.; Soares da Costa, Tatiana P.; Pietersz, Geoffrey A.; Baker, Ross I.; Hogarth, P. Mark title: Fc Binding by FcγRIIa Is Essential for Cellular Activation by the Anti-FcγRIIa mAbs 8.26 and 8.2 date: 2021-10-25 journal: Front Immunol DOI: 10.3389/fimmu.2021.666813 sha: f41b877565791402c51cfa6882a04a67c5d14b6f doc_id: 22975 cord_uid: yh0103ws FcγR activity underpins the role of antibodies in both protective immunity and auto-immunity and importantly, the therapeutic activity of many monoclonal antibody therapies. Some monoclonal anti-FcγR antibodies activate their receptors, but the properties required for cell activation are not well defined. Here we examined activation of the most widely expressed human FcγR; FcγRIIa, by two non-blocking, mAbs, 8.26 and 8.2. Crosslinking of FcγRIIa by the mAb F(ab’)(2) regions alone was insufficient for activation, indicating activation also required receptor engagement by the Fc region. Similarly, when mutant receptors were inactivated in the Fc binding site, so that intact mAb was only able to engage receptors via its two Fab regions, again activation did not occur. Mutation of FcγRIIa in the epitope recognized by the agonist mAbs, completely abrogated the activity of mAb 8.26, but mAb 8.2 activity was only partially inhibited indicating differences in receptor recognition by these mAbs. FcγRIIa inactivated in the Fc binding site was next co-expressed with the FcγRIIa mutated in the epitope recognized by the Fab so that each mAb 8.26 molecule can contribute only three interactions, each with separate receptors, one via the Fc and two via the Fab regions. When the Fab and Fc binding were thus segregated onto different receptor molecules receptor activation by intact mAb did not occur. Thus, receptor activation requires mAb 8.26 Fab and Fc interaction simultaneously with the same receptor molecules. Establishing the molecular nature of FcγR engagement required for cell activation may inform the optimal design of therapeutic mAbs. FcgR activity underpins the role of antibodies in both protective immunity and autoimmunity and importantly, the therapeutic activity of many monoclonal antibody therapies. Some monoclonal anti-FcgR antibodies activate their receptors, but the properties required for cell activation are not well defined. Here we examined activation of the most widely expressed human FcgR; FcgRIIa, by two non-blocking, mAbs, 8.26 and 8.2. Crosslinking of FcgRIIa by the mAb F(ab') 2 regions alone was insufficient for activation, indicating activation also required receptor engagement by the Fc region. Similarly, when mutant receptors were inactivated in the Fc binding site, so that intact mAb was only able to engage receptors via its two Fab regions, again activation did not occur. Mutation of FcgRIIa in the epitope recognized by the agonist mAbs, completely abrogated the activity of mAb 8.26, but mAb 8.2 activity was only partially inhibited indicating differences in receptor recognition by these mAbs. FcgRIIa inactivated in the Fc binding site was next co-expressed with the FcgRIIa mutated in the epitope recognized by the Fab so that each mAb 8.26 molecule can contribute only three interactions, each with separate receptors, one via the Fc and two via the Fab regions. When the Fab and Fc binding were thus segregated onto different receptor molecules receptor activation by intact mAb did not occur. Thus, receptor activation requires mAb 8. 26 Fab and Fc interaction simultaneously INTRODUCTION IgG Abs, elicited by vaccination or natural infection or as therapeutic mAbs, are important mediators of human health and the activation of FcgR-expressing innate leucocytes is often fundamental to their efficacy. IgG antibodies have proved themselves highly useful therapeutics, a trend that continues with almost 90 new IgG therapeutics reported in late-stage clinical trials in 2020 (1) . The family of FcgRs vary in their affinity for ligand, their structures and organization, tissue expression and the types of functions they trigger, altogether equating to diverse roles in normal immunity (2) (3) (4) (5) (6) (7) (8) . FcgRIIa binds monomeric IgG with low (~10 6 M -1 ) affinity (9, 10) and activates cells only by avid interaction with IgG immune complexes (IC) or IgG opsonized targets (11) . The activating FcgRs, including FcgRIIa, signal via an immunoreceptor tyrosine-based activation motif (ITAM) (12) , with FcgRIIa and FcgRIIc containing their ITAM within their cytoplasmic domains, while other activating FcRs associate with an ITAM containing signal transduction subunit, FcRg (13, 14) . FcgRIIa has two major polymorphisms, H131, the only functional FcgR for human IgG2, and R131 which was characterized functionally by its preferential interaction with mouse IgG1 (15, 16) . FcR activation occurs upon receptor clustering leading first to transphosphorylation of the cytoplasmic domains of receptors and/or their subunits by a pre-associated src-family kinase (17) . Depending on the effector cell stimulated, cellular responses to FcgRIIa activation may include, phagocytosis, respiratory burst, degranulation, cytokine production, mediator release and antigen presentation (2) (3) (4) (5) (6) (7) (8) . FcgRIIa is the most widely expressed FcgR of human leukocytes and the only FcgR on platelets (18) . FcgRIIa potently activates platelets when engaged by the Fc of anti-platelet antibodies (19, 20) , including antiplatelet factor 4 (PF4) antibodies which trigger heparin induced thrombocytopenia (21, 22) . FcgRIIa also triggers a rare thrombotic thrombocytopenia induced by anti-PF4 antibodies after vaccination for SARS CoV-2 spike vectored by a recombinant adenovirus (ChAdOx1, AstraZeneca) (23, 24) . The molecular basis for ligand (IgG) recognition by FcgRs (25) (26) (27) (28) , including FcgRIIa (29) , has been well defined by structural studies. However, despite the importance of this event and its intensive structural investigation, relatively little is known of the requirements for the engagement of FcgRs for optimal cell activation (30) . Some mAbs can themselves also activate FcgRIIa although the properties of these mAbs are not defined. We previously used two agonistic and non-blocking anti-FcgRIIa mAbs, mAb 8.26 and mAb 8.2 to explore FcgRIIa activation (31) . These non-blocking anti-FcgRIIa mAbs activate FcgRIIa and mAb 8.2 has been used in a number of studies (32, 33) including high resolution SIM (structural illuminated microscopy) that found mAb 8.2 treatment drives FcgRIIa into aggregates on the cell surface (32) . Using defined ligands and mutants of FcgRIIa we now investigate the essential features of FcgRIIa engagement by these mAbs that are necessary for receptor signaling and cell activation. The human FcgRIIa specific murine mAbs 8.2 (IgG1), 8.7 (IgG1), 8.26 (IgG2b) (31), IgG1 isotype control mAb X68 (34) and the production of F(ab') 2 regions (31) are as described previously. Albumin, bovine Fraction V and other fine chemicals were from Sigma-Aldrich. Stain-free SDS-PAGE gels and PVDF transfer membrane were from BioRad Laboratories (Melbourne, Australia) and 3,3′,5,5′-tetramethylbenzidine (TMB) ELISA substrate was from Life Technologies (Thermo Fisher, Melbourne, Australia). The expression vectors for unmodified wild-type (WT) and mutant chimeric (murine V domain-anti-TNP/human constant) IgG are as described previously (35, 36) . Recombinant anti-TNP heavy WT and double mutant S267E, L328F IgG1 (SELF) (37) and light chains in pCR3 (Invitrogen) were expressed by co-transfection in Expi293F ™ cells (Gibco Life Technologies) according to the manufacturer's instructions. The antibodies were purified by protein A chromatography followed by gel filtration on a Superose 6 column performed using an AKTA Purifier (GE Healthcare Life Sciences, Melbourne, Australia) (35) . WT and mutant FcgRIIa expression used cDNA [FcgRIIa; clone Hu3.0 (38)] templates and PCR reactions were performed using mutagenic primers and polymerases Pwo (Roche) or AccuPrime ™ Pfx (Invitrogen, Life Technologies). Other standard DNA manipulation used enzymes from New England Biolabs. Sequencing and expression of FcgRIIa pMX based vectors in IIA1.6 cells (39) and rsFcgRIIa-hexahistidine-AviTag-biotin (36) were as described previously. FcgRIIa capture used mAb 8.7 or mAb IV-3 coated at 20 µg/ml to F96 Maxisorp plates (Nunc) which were then blocked with PBS containing 1% BSA to each well (130 µl). The epitopes of the mAbs 8.7 and IV−3 (29, 31) differ from that of mAbs 8.2 and 8.26 defined in this study. IIA1.6 cells expressing the appropriate receptor were cultured in RPMI-1640 or DMEM medium supplemented with 5% fetal bovine serum and 2 mM glutamine, added to a round bottom plate (2x10 5 cells/well; >95% viable), plates were centrifuged (1000 rpm; 5 min) and most of the media removed by a single rapid inversion of the plate. Plates were warmed on an aluminum-foil mat heating block (37°C; 2 min). Receptor stimulation was initiated by adding 20 µl of agonist in tissue culture media. At the indicated times, reactions were stopped by adding lysis buffer (100 ul; 20 mMTris, 150 mM NaCl, 2 mM NaF pH7.4 containing 1% Brij96, 1 mM activated Na 3 VO 4 and a protease inhibitor cocktail without EDTA (Sigma Aldrich/Roche)). Lysates were then transferred to the mAb 8.7 or mAb IV-3 coated capture plate and incubated overnight at 4°C or 2h at 37°C. Plates were emptied by inversion and filled with 130 µl wash buffer (220 mM Tris, 150 mM NaCl, 2 mM NaF pH7.4, 0.05% Tween 20) supplemented with activated Na 3 VO 4 (1 mM) emptied and 2 µg/ml biotinylated anti-phosphotyrosine mAb 4G10 (4G10-biotin) in wash buffer containing 1 mM activated Na 3 VO 4 and 1% BSA added and incubated for 2h at 25°C. Plates were emptied by inversion and filled with 130 µl wash buffer supplemented with 1 mM activated Na 3 VO 4 , emptied and incubated with a 1/6000 dilution of streptavidin-HRP conjugate (Cytiva/Amersham) for 1h at 25°C. Unbound conjugate was removed by 4 cycles of emptying the wells and refilling with wash buffer and emptying again. The bound conjugate was detected by incubation with TMB substrate (typically 10 min), stopping with 1M HCl and recording the absorbance at 450 nm. Phosphorylation curves were constructed with subtraction of the zero-time point, which was typically an A450nm < 0.05 after subtraction of the reagent blank. Platelet rich plasma (PRP) was prepared by collecting whole blood into 3.2% (w/v) trisodium citrate and removing the cellular components by centrifugation (120g, 20 min, all steps 22°C). Activation was performed by incubating 20 µl PRP with 100 µl of 10 mM Tris, 150 mM NaCl pH 7.4 containing mAb, whole or F (ab') 2 for 30 min, or with the control platelet activator ADP (20 µM; 10 min). Staining was performed without washing, using 20 µl of mouse anti-P-selectin (AK-4) phycoerythrin-conjugate or mouse anti-human CD41a phycoerythrin-conjugate (HIP8; BD Pharmingen, 30 min). Stained platelets were collected by centrifugation (2 min, 300g) and resuspended in 10 mM Tris, 150 mM NaCl, 5 mM EDTA pH 7.4 two times. Platelets were visualized by flow cytometry with a forward and side scatter gate confirmed by CD41a staining. MAbs 8.7 (IgG1) F(ab') 2 and intact IV-3 were coupled to cyanogen bromide activated Sepharose according to the manufacturer's instructions (GE Life sciences). IIA1.6 cells expressing FcgRIIa were cultured in RPMI-1640 or DMEM medium as above. Cells were adjusted to 4x10 6 cells/40 µl and at the indicated times, 20 µl of the agonist mAbs (1-20 µg/ml in RPMI-1640 culture medium) were added. Reactions were terminated by the addition of 200 µl of lysis buffer (as above), incubated on ice (15 min), and then clarified by centrifugation (10000 rpm, 10 min at 4°C). An aliquot (10 µl) of clarified lysate was reserved for analysis and the remainder was incubated with 20 µl of mAb 8.7 F(ab') 2 coupled beads, 16h at 4°C. Beads were collected by centrifugation (5000 rpm, 10s) and resuspended in wash buffer. After 4 rounds of collection and resuspension in wash buffer the FcgR immune precipitates or 10 µl whole cell lysate samples were resolved on 5-15% polyacrylamide gels stain free gels (BioRad Laboratories) by SDS-PAGE and transferred to PVDF membranes. Membranes were probed with 4G10-biotin/ streptavidin-HRP to detect phospho-proteins, treated with 0.02% sodium azide to quench the HRP and washed, before reprobing with rabbit anti-Syk (N19, Santa Cruz), or anti-FcgRIIa ectodomain specific rabbit anti-serum/anti-rabbit IgG-HRP. MAbs and F(ab') 2 fragments were biotinylated using EZ-link biotinylation reagent (Pierce) according to the manufacturer's instructions. Cells (5x10 4 ) were incubated with 2 µg/ml mAbs or F(ab') 2 in FACS buffer (Hanks balanced salt solution containing 1mM glucose and 0.1% BSA), on ice for 30 min. Wells or tubes were filled with FACS buffer and cells collected by centrifugation, (1000rpm, 4°C, 5 min) and resuspended in 1/400 dilution of APC or PE conjugated streptavidin. Following incubation on ice for 30 min, wells or tubes were filled with FACS buffer and cells collected by centrifugation, 1000rpm, 4°C, 5 min and resuspended in FACS buffer for analysis using a Canto II flow cytometer (Becton Dickinson). Two methods for the measurement of FcR ligand binding activity were used, first TNP-BSA (~5 TNP groups per BSA) in 100 mM Na 2 HCO 3 was labelled with Alexa-647 according to the manufacturer's instructions and extensively dialyzed against PBS. Alexa 647labelled TNP-BSA (1 µg/ml) was reacted with 1-10 µg/ml chimeric anti-TNP (human IgG1 constant domains) described previously (40) . Second, crosslinked and biotinylated IgG was used as IC as described in (41) . The binding of anti-TNP chimeric IgG heavy chain double mutant S267E, L328F (IgG1-SELF) was detected with APClabelled AffiniPure F(ab') 2 fragment of goat anti-human IgG-F (ab') 2 fragment specific, (Jackson ImmunoResearch Laboratories Inc., Baltimore, USA). The apparent affinity of the intact mAbs and their F(ab') 2 fragments for FcgRIIa was measured using an Octet RED96e (Forté Bio). RsFcgRIIa-WT or rsFcgRIIa-RA, with c-terminal biotin was immobilized using streptavidin (SA) biosensor tips (Forté Bio) in PBS pH 7.4 containing 0.1% (w/v) BSA and 0.05% (v/v) TWEEN-20) at 25°C. Kinetic measurements made by submerging the sensors in two-fold dilution series of the mAbs or their F(ab') 2 fragments. Association and dissociation were measured for 600s and 3-6 cycles of regeneration used 2.5 M guanidinium hydrochloride, 8% isopropanol between binding reactions. While theoretically a 1:1 model is not ideal for fitting bivalent IgG or F(ab') 2 binding data, other models did not improve curve fits and apparent binding avidities (K app D ) are reported from global fits to the 1:1 model (Octet Data Analysis 10.0 software). Calcium mobilization in receptor expressing IIA1.6 cells utilized Fura-2 reporter (Abcam, Melbourne, Australia) as previously reported with the addition of probenecid (2.5 mM) to the measurement buffer (39) . Loaded cells (4x10 6 /180 µl per well) were treated with 20 µl of 200, 100, or 50 µg/ml agonist mAb 8.2 or 8.26 (final volume 200 µl) and calcium flux, the ratio of A520nm obtained with 340 and 380nm excitation, was measured using a FlexStation-3 (Molecular Devices, LLC. CA USA). Platelet activation responses ( Figure 4D The anti-receptor agonist mAbs 8.26 and 8.2 bind and activate the low affinity Fc-gamma receptor FcgRIIa. FcR deficient IIA1.6 cells were transduced to express FcgRIIa-H131 as an experimental system for exploring the requirements for receptor activation by these mAbs. Receptor activation was determined by detecting FcgRIIa phosphorylation in a Western blot wherein receptor and receptor associated proteins were captured by plate-bound mAb 8.7 ( Figures 1A-C) . Treatment of IIA1.6-FcgRIIa cells with agonist mAb 8.2 resulted in phosphorylation of the receptor and associated proteins (e.g., pSyk, Figure 1B ) with similar kinetics to that found in a conventional immunoprecipitation and Western analysis (Supplementary Figure 1) . Calcium mobilisation is a more distal event downstream of the initial receptor phosphorylation. Both mAb 8.26 and mAb Figures 1D, E) . MAb 8.2 was the more effective agonist by both receptor phosphorylation (Supplementary Figure 1 ) and calcium flux ( Figures 1D, E) . Both mAb interactions with their epitopes on FcgRIIa and the engagement of their Fc portions with the receptor may contribute to receptor activation by the mAbs 8.2 and 8.26. The contribution of each was examined using mutant receptors with abrogated mAb Fc or Fab binding. Fc binding activity of the receptor was inactivated by mutating the essential tryptophan sandwich (W87A, W110A) and Fab binding by the R55A mutation in domain 1. R55 was determined to be a key residue of the epitope for mAb 8. Figure 2A ). The anti-FcgRIIa mAbs 8.7 and IV-3 detect different distinct FcgRIIa epitopes and were used together to show the otherwise normal epitope expression by these mutant receptors and further demonstrate equivalent cell surface expression of these mutant receptors to FcgRIIa-WT ( Figure 2 and Supplementary Figure 2 ). Thus, this mutant FcgRIIa-WAWA binds the agonist mAbs 8.2 and 8.26 through their Fabs but, as expected (29), is ineffective in binding via the Fc. The WAWA mutation, in addition to abrogating Fc binding (Figure 2A) , also partially disrupts the epitope of mAb 8.7 but not that of mAb IV-3 ( Figures 2B, c.f. C) . Similarly, flow cytometric analysis indicated the R55A mutation in domain 1 of FcgRIIa abrogated mAb 8.2 Fab binding ( Figure 2D) , despite the overall similar expression of the mutant receptors indicated by the equivalent binding of the F(ab') 2 fragments of mAbs IV-3 to all mutant receptors (Figure 2 and Supplementary Figure 2) . The R55A mutation is distant from the Fc ligand binding site in the second ectodomain and, as expected, did not affect the binding of IgG IC (Supplementary Figure 3) . Further characterization of mutant FcgRIIa-R55A used immobilisation of the c-terminal biotinylated WT and R55A receptor ectodomains on BLI streptavidin biosensors. This format, reflects the orientation of interactions of the mAbs with the cell surface receptors with the F(ab') 2 fragments of the mAbs binding avidly to the immobilised FcgRIIa-WT. However, the very slow dissociation of the mAbs and the time frame of the experiments means that the dissociation rates are likely suboptimally quantitated (42) . None-the-less the apparent avidity of the F(ab') 2 fragment of 8.2 for FcgRIIa-WT (K app D < 1 pM) was at least 300-fold stronger than that of the F(ab') 2 fragment of 8.26 (K app D = 295 pM, c.f. Figures 3A, B) , while binding to the mutant Figure 4A ) consistent with the lack of its F(ab') 2 fragment binding to rsFcgRIIa-RA ( Figure 3C ). In contrast despite the lack of mAb 8.2 F(ab') 2 fragment binding to rsFcgRIIa-RA ( Figure 3D ) the intact mAb 8.2 induced poor but detectable phosphorylation of FcgRIIa-RA ( Figure 4B ) which was much reduced compared with FcgRIIa-WT, had slower kinetics and never reached the peak pY levels that FcgRIIa-WT achieved after 2 min stimulation ( Figure 4B) . BLI was performed to test for the presence of aggregates in the mAb 8.2 IgG. Size exclusion chromatography (SEC) resolved mAb 8.2 as a symmetrical peak and the leading and trailing fractions ( Supplementary Figures 4A, B) were analyzed using rsFcgRIIa-RA immobilized sensors. In contrast to the lack of binding of the 8.2 F(ab') 2 fragment to rsFcgRIIa-RA ( Figure 3D ) the intact 8.2 bound strongly (Supplementary Figures 4C, D) . If present, preformed aggregates would occur in the leading fractions of the SEC peak, however the binding activity was largely equivalent in the leading (Supplementary Figure 4C) and trailing (Supplementary Figure 4D ) peak fractions. Hence preformed aggregates of mAb 8.2 do not account for the residual binding and so receptor stimulating activity of mAb 8.2 with FcgRIIa-RA. Notably the intact mAb 8.26 had comparatively little binding activity with FcgRIIa-RA (Supplementary Figure 4E) and a higher concentration series of an isotype control IgG1, as expected, showed micromolar binding affinity (Supplementary Figure 4F) . Thus, as expected the Fab:epitope interaction, as defined using the RA mutation, is essential for the activating function of mAb 8.26. This interaction with R55 was required for the optimal binding and stimulating activity of mAb 8.2, but nonetheless was not essential. When IIA1.6 cells expressing Fc binding-inactive FcgRIIa-WAWA were treated with mAb 8.26 or mAb 8.2 no receptor associated pTyr was detected over the background levels measured in mock treated FcgRIIa-WT cells (Figures 4A, B) . This result was extended by the treatment of IIA1.6 cells expressing FcgRIIa-H131 with mAb 8.2 F(ab') 2 fragments or 8.26 F(ab') 2 fragments, which similarly resulted in no receptor associated phosphorylation above background, contrasting with the activity of the intact antibodies (8.2 IgG c.f. F(ab') 2 p = 0.005; 8.26 IgG c.f. F(ab') 2 p = 0.0007) ( Figure 4C) . Thus, engaging up to two FcgRIIa-WAWA molecules by intact mAb 8.2 or 8.26, or similarly engaging two FcgRIIa-WT by their F(ab') 2 fragments is insufficient for receptor activation. This indicates an essential role of the mAb Fc portion in activation of the cellular receptor by these anti-FcgRIIa mAbs. Next the role of the Fc in agonism was examined on human platelets which endogenously express only FcgRIIa (18) . Treatment of platelets with the mAbs 8.26 or 8.2, but importantly not their (Fab)' 2 fragments, lead to platelet activation as measured by the increased surface expression of P-selectin (Supplementary Figure 5) , with an EC 50 of~0.6 µg/ml. ( Figure 4D ). These data from IIA1.6 cells expressing FcgRIIa and from platelets together reveal that the Fc portions of these mAbs are essential for agonism of FcgRIIa in systems with both ectopically and natively expressed FcgRIIa. The Fc can bind to a receptor on the same cell as the Fab portion(s) bind receptor(s), a cis interaction, or the Fc can bridge to a receptor on a different cell, a trans interaction. The nature of the Fc interaction within the mAb 8.2: FcgRIIA complex formed on the cell was investigated using a competitive binding assay with the mAb 8.2 or its F(ab') 2 fragment and human IgG1-S267E, L328F (IgG1-SELF), a monomeric high affinity Fc ligand of the R131 allele of FcgRIIa (37) . In the presence of mAb 8.2 the capacity of IgG1 SELF ligand binding to FcgRIIa-R131 (B max ) was reduced~2-fold from MFI (x10 3 ) = 77 to 39 (p < 0.0001) ( Figure 4E) . In contrast the F(ab') 2 had little effect, trending towards increase, on the number of ligand binding sites on the cell (B max , MFI (x10 3 ) = 87, p = ns). The decreased number of ligand binding sites with bound intact mAb 8.2 is consistent with the Fc portion binding FcgRIIa and competitively inhibiting binding of the high affinity ligand IgG1-SELF. Thus, each mAb molecule binds to FcgRIIa by its two non-blocking Fabs, with its Fc also engaging a receptor, and the halving of the number of available IgG1-SELF ligand binding sites on the mAb 8.2 treated cells ( Figure 4E ). These interactions with cellular FcgRIIa are shown schematically in ( Figure 4F ) indicating each agonist mAb can participate in three possible stable interactions with receptors (2x Fab + 1x Fc), which at binding saturation of all the cellular receptors results in the Fc interactions occupying half the ligand binding sites (FcgRIIa) of the cell. This also suggests that such tripartite binding is predominately a cis interaction on the cell surface and may be a requirement for the FcR activation by these mAbs. This could not be achieved by a binding dominated by trans interactions which are limited to receptors at points of contact between cells. Since the Fab and Fc interactions are non-competitive, crosslinking of receptors into large complexes is likely (note unoccupied ligand binding sites 'L' and epitopes 'E' in Figure 4F ). Since FcgRIIa stimulation by the mAbs requires participation of both the Fab and Fc portions and since the intact mAbs exhibit tripartite F(ab') 2 + Fc binding we tested whether mAb stimulated receptor activation would still occur if these interactions were segregated onto different receptor molecules ( Figure 5A ). Fab and Fc binding was segregated to FcgRIIa-WAWA and FcgRIIa-RA co-expressed in the same cell. Staining of these co-expressing cells with mAb 8.2 (Fab) and mAb 8.7 confirmed the simultaneous expression of FcgRIIa-WAWA and FcgRIIa-RA ( Figure 2E ). While FcgRIIa-WAWA exclusively binds the mAbs 8.2 and 8.26 F(ab') 2 fragments, a competitive IC binding assay was used to confirm the participation of the mAb Fc in binding to the FcgRIIa-RA on these co-expressing cells (Supplementary Figure 6) . As expected, IgG immune complex binding to FcgRIIa-RA expressing cells was not inhibited by mAb 8.26, either as intact IgG or as F(ab') 2 fragments, since binding is abrogated to the disrupted epitope ( Figure 5B) . A small inhibition of IC binding was observed with intact mAb 8.2 IgG ( Figure 5B ), again suggesting this mAb retains some interaction with the RA mutant receptor. As expected, there is no IC binding to the FcgRIIa-WAWA cells ( Figure 5C ), but IC binding occurs as expected to the coexpressed FcgRIIa-RA/FcgRIIa-WAWA. Notably the intact IgG form of the mAbs 8.26 and 8.2 markedly inhibit IC binding (****, p < 0.0001), while their F(ab') 2 fragments are ineffectual. Thus, in these cells, when the agonist mAb binds the unaltered epitope of FcgRIIa-WAWA molecules via its two Fabs, its Fc can also bind co-expressed FcgRIIa-RA ( Figure 5A ) and so inhibit IC binding ( Figure 5C ). Having demonstrated Fab binding to FcgRIIa-WAWA and Fc binding to co-expressed FcgRIIa-RA the ability of mAb to activate these complexes was determined. MAb 8.26 did not elicit receptor-associated phosphorylation above background in the FcgRIIa-WAWA/FcgRIIa-RA mutant co-expressing cells ( Figure 6A) . Furthermore, mAb 8.2 induced only weak receptor-associated phosphorylation in the FcgRIIa-WAWA/ FcgRIIa-RA co-expressing cells ( Figure 6B ) which was similar to that observed in the FcgRIIa-RA cells (Figures 4B, 6C) . This weak receptor-activation was similar, both in terms of the reduced levels of phosphorylation and in its delayed kinetics ( Figure 4B cf. Figures 6B, C) . The poor but detectable phosphorylation induced by mAb 8.2, is again attributed to the residual binding to the FcgRIIa-R55A, as observed in the BLI analysis (Supplementary Figure 4) . Thus, co-expression of the FcgRIIa-WAWA/FcgRIIa-RA mutants did not result in rescue of anti-FcgRIIa mAb receptor phosphorylation since the mAb 8.2 induced a similar weak receptor-activation in the cells expressing FcgRIIa-RA alone. Lastly, mAb activation of FcgRIIa-WT and FcgRIIa mutant receptor expressing cells was assessed by calcium mobilisation, as a sensitive measure of events distal to receptor phosphorylation. The two agonist mAbs induced robust calcium flux in FcgRIIa-WT expressing cells but, consistent with the phosphorylation studies, neither mAb 8.26 nor 8.2 induced calcium mobilisation in FcgRIIa-WAWA cells, confirming the necessity for Fc engagement for the mAb activity ( Figures 7A, B) . MAb 8.26 treatment showed negligible activation of either FcgRIIa-RA expressing or FcgRIIa-WAWA/FcgRIIa-RA co-expressing cells ( Figure 7A) . Thus, the complex formed on the FcgRIIa-WAWA/FcgRIIa-RA co-expressing cells, comprising of one-mAb engaged in up to three interactions with receptors (2x Fab + 1xFc), was ineffective in receptor activation. Furthermore, Fc and Fab binding to the same FcgRIIa molecule is required for the mAb 8.26 stimulated receptor activation. MAb 8.2 induced calcium fluxes that were similarly reduced in peak height and area in both the FcgRIIa-RA single expressing and FcgRIIa-WAWA/FcgRIIa-RA co-expressing cells ( Figures 7B, C) . These equivalent muted calcium fluxes, mirror the reduced levels of receptor phosphorylation, and together indicate that, for this mAb also, optimal activation is not restored upon co-expression of the two mutant receptors (i.e., FcgRIIa-WAWA with FcgRIIa-RA). The recognition by FcgRs of IgG complexed with antigen, activates receptor bearing cells and so specifically directs innate effector cells to targets of adaptive humoral immunity (2) (3) (4) (5) (6) (7) 43) . In this study we explored FcgR activation by two agonistic and non-blocking anti-FcgRIIa mAbs, mAb 8.26 and mAb 8.2. BLI analysis revealed high apparent binding avidities for the bivalent F(ab') 2 fragments, with apparent avidities of~300 pM for the weaker activating mAb 8.26 and < 1 pM for the stronger activator mAb 8.2. The epitopes of both mAb 8.26 and mAb 8.2 were disrupted by the R55A mutation in the first extracellular domain, ablating binding of the F(ab') 2 fragments of both 8.26 and 8.2, but leaving residual Fc-dependent binding of the intact mAb 8.2. The activation of FcgRIIa-RA was completely abrogated for mAb 8.26, and largely abrogated for mAb 8.2. For mAb 8.2 residual activation was consistent with the residual binding activity of this intact mAb for the epitope mutated receptor. This binding of mAb 8.2 to FcgRIIa-RA indicates its recognition of FcgRIIa differs from mAb 8.26, despite R55 being a key feature of the epitopes of both mAbs. Receptor activation by both mAb 8.26 and 8.2 depends upon Fab binding to epitopes that do not inhibit the ligand binding function of the receptor, thus allowing their Fcs to also bind FcgRIIa. Indeed, receptor activation by these mAbs required the mAb-Fc to interact with the receptor since their F(ab') 2 fragments did not activate cells, and cells expressing the mutant FcgRIIa-WAWA, which is inactive in Fc binding, was not activated by either intact mAb 8.26 or 8.2. Thus, the binding of two receptors, two WT receptors by F(ab') 2 fragment or two FcgRIIa-WAWA mutant receptors by intact mAb, is insufficient for signalling. Furthermore, this failure of the intact mAbs to elicit functional activity from FcgRIIa-WAWA indicated that Fc : Fc interactions, that promote the ordered aggregation of antibodies in antigen:IgG complexes (44, 45) are, not sufficient for activation by these mAbs. That is, if Fc : Fc interactions do occur, in addition to Fab : FcgRIIa interactions with these mAbs, these are insufficient for receptor activation. Rather Fc : FcgRIIa and Fab : FcgRIIa interactions are required for activation. Having shown that receptor activation by these agonist mAbs required both Fab and Fc interaction we tested if these interactions would still stimulate receptor activation when segregated to separate receptor molecules. The co-expression of mutant receptors was used to test this engagement of separate receptors with only Fab and only Fc binding activity. FcgRIIa-RA lacks mAb 8.26 and 8.2 F(ab') 2 fragment binding but binds the Fc normally. However, coexpression of this FcgRIIa-RA with FcgRIIa-WAWA, lacking Fc binding activity, was not permissive of receptor activation by the mAb 8.26. The mAb 8.26 : FcgRIIa-RA : FcgRIIa-WAWA-RA : FcgRIIa-WAWA complex formed on these cells will comprise one-mAb contributing up to three interactions with receptors (2x Fab + 1x Fc), but this was ineffective in receptor activation. The requirement for Fab and Fc interactions on the same FcgRIIa molecule indicates that further crosslinking of receptors is necessary for activation. MAb 8.2 mediated stimulation was similarly not rescued with the co-expression of FcgRIIa-RA/ FcgRIIa-WAWA receptors. The similarly muted calcium fluxes and receptor phosphorylation seen when FcgRIIa-RA alone was treated with 8.2, suggests mAb 8.2 and 8.26 differ in some aspect of their manner of receptor binding and stimulation. The key role of the FcR : Fc interaction in activation by the mAbs is notable as the mAb 8.2 is a mouse IgG1 subclass which is a poor Fc ligand of the human FcgRIIa-His131 allelic form used in the receptor activation studies herein (15, 16) . Intact mAb is likely to avidly bind by its Fabs (K app D~p M for F(ab') 2 by BLI) to receptors on the cell surface and so co-localise and present the Fc portion for binding in cis. Indeed, cell binding analysis ( Figure 4E ) showed the mAb 8.2 presented Fc engages with receptor and, despite this interaction only having micromolar intrinsic affinity (9), does so sufficiently strongly to inhibit the binding of a high affinity mutant human IgG1 [S267E, L328F, K D of~4 nM (37) ] to half the receptors (FcgRIIa-R131) of the cell. Although some trans mAb interactions bridging between cells may occur, the occupation of half the ligand binding sites on the mAb saturated cells appears consistent with largely cis interactions on the same cell. In this way, even intrinsically low affinity interactions are favoured by the apparent high local concentration of ligand, in this case the colocalised Fc of the agonistic mAbs. This phenomenon, known as the "scorpion" or Kurlander effect (6, 46) , can enhance apparent interaction affinities by many orders of magnitude (47) . Thus, the low intrinsic affinity of mouse IgG1-Fc interaction with FcgRIIa-His131 does not preclude its critical importance in the activation of FcgRIIa expressing cells by mAb 8.2. More broadly this shows a Fc : FcR interaction that in itself is normally too weak to bind appreciably can be sufficient to result in Fc : FcR interaction, when appropriately co-localised to the cell surface by Fab binding, and can thus initiate cell activation and biological responses. This has important implications for the possibility of low affinity FcR-dependent mechanisms of action of other antibodies that target cells bearing FcgRs and have low, but not completely abrogated, FcgR binding activity. Unforeseen functional responses dependent on very weak FcgRII interactions have triggered adverse reactions to therapeutic mAbs under other circumstances (48) or confounded mAb therapy by modulation of the target molecule (49) (50) (51) . Other studies are compatible with the conclusion herein that small immune complexes are unable to activate FcgRs. Recombinant trimeric human IgG-Fc molecules have been developed recently which effectively bind, but do not activate FcgRs, and so are inhibitors of FcgR function. These molecules had therapeutic efficacy in several mouse models of autoimmune antibody mediated pathology (52) . In other studies, engineered IgGs comprising two or three Fc regions were not activating in themselves but had avidity enhanced potency over normal IgG when aggregated on a target (53) (54) (55) . Recent imaging studies found a pentameric Fc-ligand recruited and activated Syk to the endosomes of FcgRI + , FcgRIIa + THP1 cells. In contrast, activation by large complexes occurred at the plasma membrane (56) . In summary, our study found activation of FcgRIIa by the nonblocking agonist mAbs 8.26 and 8.2 required interactions with the Fab and the Fc on the same receptor molecule, which enables signalling permissive receptor crosslinking. MAb interactions limited to only two (e.g., F(ab') 2 fragment) or three receptors did not lead to receptor activation. These findings, applied more generally, will assist in the development of more 'tuneable' therapeutic mAbs. The raw data supporting the conclusions of this article will be made available by the authors. The studies involving human participants were reviewed and approved by Alfred Hospital Ethics Committee. The patients/ participants provided their written informed consent to participate in this study. BW and PH conceived and designed the experiments. BW, PH, GM, HT, SE, RI, TC, RA, and RB designed assays. BW, HT, SE, RI, GP, and TC performed experimental work and analyzed results. BW, PH, TC, and GM drafted the manuscript. All authors contributed to the article and approved the submitted version. This work was supported by the Australian NHRMC -Project Grant, to PH and BW (GNT1145303). Support to the Burnet Institute comes from the NHMRC Independent Research Institutes Infrastructure Support Scheme and a Victorian State Government Operational Infrastructure grant. TC holds an Australian Research Council fellowship (DE190100806). Antibodies to Watch in 2021 Anti-Retroviral Antibody FcgR-Mediated Effector Functions The Role and Function of Fcg Receptors on Fc-Receptor Interactions Regulate Both Cytotoxic and Immunomodulatory Therapeutic Antibody Effector Functions Time for Large Eaters: Monocytes and Macrophages as Effector and Target Cells of Antibody-Mediated Immune Activation and Repression Fc Receptor-Targeted Therapies for the Treatment of Inflammation, Cancer and Beyond FcgammaR Dependent Mechanisms of Cytotoxic, Agonistic, and Neutralizing Antibody Activities The Function of Fcgamma Receptors in Dendritic Cells and Macrophages Specificity and Affinity of Human Fcgamma Receptors and Their Polymorphic Variants for Human IgG Subclasses Biochemical Analysis and Crystallisation of FcgRIIa, the Low Affinity Receptor for IgG The Human FcgRII (CD32) Family of Leukocyte Fcr in Health and Disease Receptor Dissociation and B-Cell Activation A Common Site of the Fc Receptor g Subunit Interacts With the Unrelated Immunoreceptors FcaRI and FceRI Clarifying the Confusion Between Cytokine and Fc Receptor A Single Amino Acid in the Second Ig-Like Domain of the Human Fcg Receptor II is Critical for Human IgG2 Binding Fc Receptors for Mouse IgG1 on Human Monocytes: Polymorphism and Role in Antibody-Induced T Cell Proliferation The Joy of Aggregation There is (Scientific) Strength in Numbers: A Comprehensive Quantitation of Fc Gamma Receptor Numbers on Human and Murine Peripheral Blood Leukocytes The Role of the Human Fc Receptor FcgRIIA in the Immune Clearance of Platelets: A Transgenic Mouse Model Platelets Release Pathogenic Serotonin and Return to Circulation After Immune Complex-Mediated Sequestration The Platelet Fc Receptor Dimeric FcgR Ectodomains Detect Pathogenic Anti-Platelet Factor 4-Heparin Antibodies in Heparin-Induced Thromobocytopenia Antibody Epitopes in Vaccine-Induced Immune Thrombotic Thrombocytopaenia Thrombotic Thrombocytopenia After Chadox1 Ncov-19 Vaccination Structure of Fcgammari in Complex With Fc Reveals the Importance of Glycan Recognition for High-Affinity IgG Binding Crystal Structure of the Human Leukocyte Fc Receptor, Fc Gammariia The Structure of a Human Type III Fcgamma Receptor in Complex With Fc The 3.2-a Crystal Structure of the Human Igg1 Fc Fragment-Fc Gammariii Complex Structural Basis for FcgRIIa Recognition of Human IgG and Formation of Inflammatory Signaling Complexes Mapping Epitopes of Human FcgRII (Cdw32) With Monoclonal Antibodies and Recombinant Receptors The Rare Anaphylaxis-Associated FcgRIIa3 Exhibits Distinct Characteristics From the Canonical FcgRIIa1 Alteration of the FcgRIIa Dimer Interface Affects Receptor Signaling But Not Ligand Binding Distinctive Expression of Interleukin-23 Receptor Subunits on Human Th17 and Gammadelta T Cells Specificity of Staphylococcal Superantigen-Like Protein 10 Toward the Human Igg1 Fc Domain Dimeric Fcgammar Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG Inhibition of B Cell Receptor-Mediated Activation of Primary Human B Cells by Coengagement of CD19 and Fcgammariib With Fc-Engineered Antibodies Molecular Cloning of a Human Immunoglobulin G Fc Receptor Fc Receptor g Chain Residues at the Interface of the Cytoplasmic and Transmembrane Domains Affect Association With FcaRI, Surface Expression, and Function Dimeric FcgR Ectodomains as Probes of the Fc Receptor Function of Anti-Influenza Virus IgG Polymorphisms and Interspecies Differences of the Activating and Inhibitory Fcgammarii of Macaca Nemestrina Influence the Binding of Human IgG Subclasses Determination of High-Affinity Antibody-Antigen Binding Kinetics Using Four Biosensor Platforms Mouse and Human Fcr Effector Functions Fc-Mediated Immune Precipitation. I. A New Role of the Fc-Portion of IgG Blockade of Fc Receptor-Mediated Binding to U-937 Cells by Murine Monoclonal Antibodies Directed Against a Variety of Surface Antigens The Magnitude of Signal Amplification by Ligand-Induced Receptor Clustering Upregulation of Fcgammariib on Monocytes is Necessary to Promote the Superagonist Activity of TGN1412 Antagonistic Human Fcgammariib (CD32B) Antibodies Have Anti-Tumor Activity and Overcome Resistance to Antibody Therapy In Vivo Expression of the Inhibitory Fc Gamma Receptor IIB (FCGR2B, CD32B) on Follicular Lymphoma Cells Lowers the Response Rate to Rituximab Monotherapy (SAKK 35/98) Fc gamma Receptor IIb on Target B Cells Promotes Rituximab Internalization and Reduces Clinical Efficacy Elucidating the Interplay Between IgG-Fc Valency and FcgR Activation for the Design of Immune Complex Inhibitors Enhanced Antibody-Dependent Cellular Phagocytosis by Chimeric Monoclonal Antibodies With Tandemly Repeated Fc Domains Tandemly Repeated Fc Domain Augments Binding Avidities of Antibodies for Fcgamma Receptors, Resulting in Enhanced Antibody-Dependent Cellular Cytotoxicity Enhancement of Antibody Functions Through Fc Multiplications Engineered Igg1-Fc Molecules Define Valency Control of Cell Surface Fcgamma Receptor Inhibition and Activation in Endosomes Conflict of Interest: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest The authors wish to thank Janine Stubbs for initial calcium experiments and discussions. The authors wish to acknowledge the expert support of the AMREPFlow core flow cytometry facility. We also thank the La Trobe University Comprehensive Proteomics Platform for providing infrastructure support. The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fimmu.2021. 666813/full#supplementary-material