key: cord-0025622-hl917s3u authors: Drzyzga, Christine J.; Bahls, Martin; Ittermann, Till; Völzke, Henry; Bülow, Robin; Hammer, Fabian; Ewert, Ralf; Gläser, Sven; Felix, Stephan B.; Dörr, Marcus; Markus, Marcello R. P. title: Lower Cardiorespiratory Fitness Is Associated With Right Ventricular Geometry and Function – The Sedentary’s Heart: SHIP date: 2021-11-06 journal: J Am Heart Assoc DOI: 10.1161/jaha.120.021116 sha: 054e03eeddfdbdcafa633468344c6a060dca2fad doc_id: 25622 cord_uid: hl917s3u BACKGROUND: Lower cardiorespiratory fitness (CRF) is associated with an increased risk for cardiovascular disease. However, very little information is available about the association between lower CRF and right ventricular (RV) remodeling. We investigated the relationship between CRF and RV structure and function in a large, aging, and largely sedentary adult population–based cohort. METHODS AND RESULTS: We used cross‐sectional data of 2844 subjects (1486 women; median age, 51 years; interquartile range, 40–62 years) from the population‐based cohort SHIP (Study of Health in Pomerania) with echocardiography, of which 941 also had cardiac magnetic resonance imaging. We analyzed the associations of peak oxygen uptake with RV parameters determined by both imaging techniques using multivariable‐adjusted linear regression models. In echocardiography, a 1 L/min lower peak oxygen uptake was associated with a 1.18 mm (95% CI, 0.66–1.71; P<0.001) smaller RV end‐diastolic diameter and a 1.41 mm (95% CI, 0.90–1.92; P<0.001) narrower RV end‐diastolic outflow tract diameter. Similarly, using cardiac magnetic resonance imaging measurements, a 1 L/min lower peak oxygen uptake was associated with a 23.5 mL (95% CI, 18.7–28.4; P<0.001) smaller RV end‐diastolic volume, a 13.0 mL (95% CI, 9.81–16.2; P<0.001) lower RV end‐systolic volume, and a 10.7 mL/beat (95% CI, 8.10–13.3; P<0.001) lower RV stroke volume. CONCLUSIONS: Our results indicate a significant association between CRF and RV remodeling. Lower CRF was associated with smaller RV chamber and lower RV systolic function, stroke volume, and cardiac output. METHODS SHIP data are publicly available for scientific and quality control purpose. Data usage can be applied for via www.community-medicine.de. 24 The presented data were derived from the populationbased prospective cohort SHIP. 25 Recruitment strategy and study design have been reported elsewhere. 24 Briefly, between 1997 and 2001 a random cluster sample of 6265 subjects (aged 20-79) was drawn from the population of West Pomerania, a region in the northeast of Germany. A total of 4308 (2193 women) subjects participated in the baseline (SHIP-0) study (response=68.8%). In the first examination follow-up (SHIP-1), which was realized between 2002 and 2006, of 3949 eligible persons, 3300 subjects were reexamined (follow-up re-sponse=83.6%). In the second examination follow-up (SHIP-2), which took place between 2008 and 2012, of 3708 eligible persons, 2333 subjects were reexamined (follow-up response=62.9%). 24 Between 2008 and 2012, while SHIP-2 was being conducted, a second independent cohort was established, called Study of Health in Pomerania (SHIP) -Trend, covering a population from the same region as SHIP. A stratified random sample of 8826 adults (aged 20-79) was selected. Subjects who participated in the initial SHIP cohort were excluded from SHIP-TREND. Thus, 4420 individuals were examined in SHIP-TREND (response=50.1%). 24 For the present study, we pooled data from SHIP-2 and SHIP-TREND (n=6753; 3510 women [52.0%]) and performed cross-sectional analyses. We excluded, sequentially, participants with previous myocardial infarction or stroke (n=367), pacemakers (n=60), left bundle block (n=51), and LV ejection fraction (determined by echocardiography) <40% (n=38). We also excluded individuals with missing values for either CRF (n=1869) or RV end-diastolic diameter (RVEDD; n=769) as well as both CRF and RVEDD (n=716) or any of the covariates (n=39) (Figure 1 ). The final sample comprised 2844 subjects with echocardiographic data (1486 women; 52.3%) aged 20 to 88 years. A subsample of 966 individuals also underwent cMRI, and data of 941 subjects with good-quality images for the RV were available for further analyses (Figure 1 ). All study participants gave written informed consent. The study was approved by the ethics committee of the University of Greifswald and complies with the Declaration of Helsinki. What Is New? • Our study demonstrates that lower cardiorespiratory fitness is associated with smaller chambers size and lower systolic function, stroke volume, and cardiac output of the right heart. • We believe that this relation might explain the previously described aging-related decrease in size of the heart wherein the sedentary lifestyle of most individuals throughout life might be the most important contributor. What Are the Clinical Implications? • Further studies are needed to identify the mechanisms of these changes in detail for a possible therapeutic use and to develop more information about the clinical relevance of the right ventricle, possibly also in different populations. CRF cardiorespiratory fitness PA physical activity RVEDD right ventricular end-diastolic diameter RVEDV right ventricular end-diastolic volume SHIP Study of Health in Pomerania VO 2peak peak oxygen uptake A symptom-limited CPET using a calibrated electromagnetically braked cycle ergometer (Ergoselect 100, Ergoline, Germany) was performed with a physician in attendance according to a modified Jones protocol (3 minutes of rest, 1 minute of unloaded cycling at 60 rpm, 1-minute increases in workload of 16 W/min until symptom-limited [volitional exertion, dyspnea, or fatigue] or terminated by the physician because of chest pain or ECG abnormalities, and 5 minutes of recovery). 26, 27 All tests were performed at room air according to current guidelines for exercise testing, with continuous monitoring of ECG, blood pressure, and pulse oximetry. Gas exchange and ventilatory variables VO 2peak was analyzed breath by breath averaged over 10-second intervals using a computer-based system called VIASYS HEALTHCARE system (Oxycon Pro, Rudolph`s mask, JAEGER/VIASYS Healthcare system; Hoechberg, Germany). 26 Exercise duration was defined from the start of exercise (without resting period) up to its termination. VO 2peak in L/min was defined as the highest 10-second average of absolute oxygen uptake during late exercise or early recovery. 26 The median time interval between the core examination and the cardiopulmonary exercise testing was 28 days (interquartile range, 9; 48 days). Two-dimensional, M-mode and Doppler echocardiography were performed by physicians (vivid-i, GE Medical Systems, Waukesha, Wisconsin, WI) as described in detail elsewhere. 28 Measurements of RVEDD, RV outflow tract diameter, pulmonary velocity acceleration time and tricuspid annular plane systolic excursion were performed according to the guidelines of the American Society of Echocardiography. 29, 30 Mean pulmonary arterial pressure was calculated in mm Hg using the following equation: mean pulmonary arterial pressure=10^ (−0.0068 * pulmonary valve acceleration time + 2.1) . Certification examinations for interobserver variations revealed an agreement of >90%. cMRI and Analysis cMRI was performed on a 1.5-T MR system (Magnetom Avanto; Siemens Medical Systems, Erlangen, Germany) as previously described. 31 Quantitative image analysis was performed by 2 observers with 3 and 5 years of MRI experience using semiautomatic tools in QMass MR 7.2 (MEDIS, Leiden, Netherlands). Interobserver variability was computed in a random subsample of 5%. Certification examinations for interobserver variations revealed an agreement of >90%. 28 Postcontrast images were interpreted of the 2 readers mentioned above and supervised in a consensus reading by a radiologist with 12 years of experience. All observers were unaware of the participants' medical history. For the RV measurements, RV end-diastolic volume (RVEDV) and RV end-systolic volume were manually traced in end diastole and end systole in transverse axis view. Volumes below the pulmonary valve were included. At the inflow tract, thin-walled structures without trabeculations were not included as part of the RV. RVEDV was determined during the first image of the acquisition. RV end-systolic volume was measured by determining the phase in which the RV intracavity blood pool was at its smallest by visual assessment at the midventricular level. RV stroke volume, RV cardiac output and RV ejection fraction were calculated following the equations below: To characterize the study sample, data are reported as the median (25th and 75th percentile) for continuous variables and as percentages for categorical variables stratified by quartiles and sex. The P for trend was calculated by univariate linear regression models with the continuous VO 2peak variable as outcome and each of the listed variables as explanatory variables. The association of VO 2peak with RV parameters was investigated by multivariable linear regression models adjusted for age, sex (not when stratified by sex), body fat mass, height 2.7 , systolic blood pressure, use of antihypertensive medication, glycated hemoglobin, use of hypoglycemic medication, smoking status, and estimated glomerular filtration rate. To evaluate the robustness of our findings in light of dropout from baseline to follow-up examination (SHIP-0 to SHIP-2) and individuals that did not take part in the echocardiographic and magnetic resonance imaging examinations, we performed inverse probability weighting, 32 assuming a missing at random mechanism, 33 based on sociodemographic and health-related variables in our analyses. We used fractional polynomials to test potentially nonlinear relationships between exposure and outcomes. 34 A 2-sided P<0.05 was considered as statistically significant. Statistical analyses were performed using Stata 14.1 (Stata Corporation, College Station, TX). Please see Data S1 for a more detailed description. The clinical and laboratory characteristics of all study participants stratified by quartiles of VO 2peak and sex are summarized in Table S1 shows the characteristics of the study sample stratified by the whole sample and the analyses sample. Although the associations of VO 2peak with echocardiographically and cMRI-determined parameters were not modified by age or sex (all P values for interaction >0.3), we decided to evaluate all the associations of VO 2peak with parameters of cardiac geometry and function not only in pooled sex analyses (the total sample) but also stratified by sex. In sensitivity analyses, we also stratified by age (Table S2 shows our results stratified by age). Table 2 shows the adjusted β-coefficients (95% CI) of the associations between VO 2peak and different RV structural and functional parameters based on echocardiography and cMRI, respectively. Since CRF (independent variable in our analysis) decreases with age 35 and we aimed to assess the relationship between CRF and RV parameters (dependent variable) in our aging and mostly physically inactive study population, we inverted the x axis. A more detailed explanation can be found in the discussion. Figure 2A illustrates the associations of VO 2peak with different echocardiographic parameters. Specifically, a 1 L/min lower VO 2peak was associated with a 1.18 mm (95% CI; 0.66-1.71; P<0.001) smaller RVEDD and 1.41 mm (95% CI, 0.90-1.92; P<0.001) narrower RV outflow tract. In addition, a 1 L/min lower VO 2peak was related with 0.97 mm Hg (95% CI, −1.71 to −0.22; P=0.011) greater mean pulmonary arterial pressure and 0.84 mm (95% CI, 0.46-1.22; P<0.001) lower tricuspid annular plane systolic excursion. There was no association between VO 2peak and lateral early and late tricuspid annular peak diastolic velocity ratio. Figure 2B shows the associations of VO 2peak with cMRI parameters. A 1 L/min lower VO 2peak was associated with a 23.5 mL (95% CI, 18.7-28.4; P<0.001) smaller Right ventricular structural parameters based on echocardiography RVEDD, mm and individuals who did not take part in the echocardiographic and magnetic resonance imaging examinations (SHIP-2 and SHIP-Trend). cMRI indicates cardiac magnetic resonance imaging; e´/a´ ratio, lateral early and late tricuspid annular peak diastolic velocity ratio; MPAP, mean pulmonary arterial pressure; RVCO, right ventricular cardiac output; RVEDD, right ventricular end-diastolic diameter; RVEDV, right ventricular end-diastolic volume; RVEF, right ventricular ejection fraction.; RVESV, right ventricular end-systolic volume; RVOT, right ventricular end-diastolic outflow tract diameter; RVSV, right ventricular stroke volume; SHIP, Study of Health in Pomerania; and TAPSE, tricuspid annular plane systolic excursion. To investigate which of the echocardiographic variables were independently of each other associated with the VO 2 peak, we conducted a linear regression analysis with VO 2 peak as outcome and all five echocardiographic variables as exposures adjusting for the same confounder than in the main analyses. In this model RVEDD (P=0.003), RV outflow tract (P<0.001) and tricuspid annular plane systolic excursion (P<0.001) were significantly associated with the VO 2 peak. For the MRI variables, such an analysis was not possible because these variables were highly correlated among each other. In this study, we evaluated associations of CRF with RV structure and function in a large sample of the general population that were mostly free of clinically relevant cardiometabolic diseases. Our main result is a positive association between VO 2peak values and RV structural (eg, RVEDD and RVEDV) as well as functional parameters (eg, tricuspid annular plane systolic excursion). To the best of our knowledge, only one previous population-based study, the MESA (Multi-Ethnic Study of Atherosclerosis), reported positive associations between PA and RV remodeling. 11 Higher levels of moderate and vigorous physical activity were linearly associated with greater RV mass and RV volumes. Further, intentional exercise was nonlinearly associated with RV mass (independent of LV mass) and with RVEDV. Study participants who spent more time doing intentional exercise also had greater RV stroke volume. However, there was no significant association between moderate and vigorous PA and RV ejection fraction as well as between intentional exercise and RV ejection fraction. A potential limitation of this analysis was that PA was assessed using self-reported questionnaires, which may induce social desirability bias and overestimate the true levels of PA. 36, 37 To exclude the mentioned bias of PA, we used CRF measured with the gold standard CPET. Further, PA and CRF are inversely and independently associated with all-cause and cardiovascular mortality. 38, 39 Physical inactivity or sedentary lifestyle is causally associated with deleterious cardiovascular risk profiles and outcomes. Importantly, the pathophysiological mechanisms involved with sedentary lifestyle are not fundamentally the opposite of those related with physical activity and exercise training. Previous studies, like the MESA, have predominantly investigated the effects of exercise training on cardiac parameters-"the athlete's heart," while only a few publications have evaluated the shrinkage of the heart following a sedentary lifestyle. The problem of this view is that, unfortunately, physical activity or exercise training is not a major characteristic of the lifestyle of the normal population. Western society tends to be more and more described as a prevalent sedentary society with all the deleterious effects that accompany this choice. Therefore, we believe that there is a lack of research that analyzes the associations of lower cardiorespiratory fitness with health parameters. Moreover, our study obtained a high agreement using our 2 methods (echo and cMRI) and we have a larger number of participants with a wider age range than MESA. While higher levels of PA may increase CRF, 38,40 our population-based cohort consists of an aging and largely physically inactive population. Hence, at least 2 interpretations of our findings are possible. One may discuss the positive association between higher levels of VO 2peak and RV structural and functional parameters. Alternatively, because of our study population, one may conclude that aging and physical inactivity may also induce RV remodeling. Older individuals have lower levels of CRF and are more sedentary than young individuals. To facilitate the interpretation of our data we changed the directionality of the x axis in our figures to clarify this interpretation. The decline in VO 2peak caused by aging is a multifactorial process. The central physiological mechanism that might explicate the finding of our study is the volume unloading of the heart caused by a decline in total blood and plasma volume. Physical inactivity reduces circulating plasma proteins and thirst resulting in reduced oncotic pressure and less fluid intake. In addition, physical inactivity seems to lead to an inhibition of the renin-aldosterone system that results in less renal sodium and water retention, which causes more urine output. All these effects, following physical inactivity, lead to a volume unload of the heart, 35 which decreases venous return and, moreover, reduces cardiac filling and stroke volume, which in turn further lowers VO 2peak . 41 In a previous analysis, 18 we found similar results regarding the LV structure and function. In addition, physical inactivity induces endothelial dysfunction as well as vasoconstriction. During aging, the skeletal muscles also atrophy, which leads to the activation of muscle proteolytic pathways via mitochondrial fission. 42 The cardiac stiffness results from a fibrous conversion of the amorphous intercellular substance, resulting in an impairment of oxygen delivery. 43, 44 Hence, our results may be explained by an inactive aging population with less plasma volume and subsequently altered RV structure. This results in a smaller and stiffer heart, particularly in an aging and inactive population. 18, 35, 45, 46 In modern societies, the prevalence of physical inactivity have reached pandemic levels. 9 Low levels of CRF, partially driven by inactivity, are associated with a higher risk of cardiovascular disease and all-cause mortality. 9, [47] [48] [49] Physical inactivity has adverse effects on the cardiovascular system (eg, a higher risk for hypertension and myocardial infarction), on the musculoskeletal system (eg, atrophy and sarcopenia) and the metabolism (eg, reduced fatty acid oxidation and glucose uptake). 48 Maintaining high levels of PA throughout life may help to reduce the age-related RV alterations. The estimated rate of decline in VO 2peak between the ages of 25 and 65 was about 40% slower in physically active compared with inactive men. 50 Physically inactive men also have a threefold greater decline in VO 2peak than individuals who were recreationally active. 51 The age-related decline in VO 2peak is not only a result of lower levels of PA because there is also a 5% decline per decade in highly active individuals. 35 Sarcopenia describes the age-related decline of muscle mass with decreased strength and aerobic and functional capacity. 9, 52, 53 The prevalence of sarcopenia is 5% to 13% in 60-to 70-year-old subjects and even 11% to 50% in subjects over the age of 80 years. 52 Reduced muscle mass leads to lower VO 2peak . 53 VO 2peak is also lower in older individuals because of reduced mitochondrial oxidative capacity in skeletal muscles. 35, 45 The interaction between aging, age-associated physical inactivity and decreased muscle mass leads to a reduction of VO 2peak . 35, 54 In summary and in line with our results, the decline in VO 2peak is related primarily to a lower maximal cardiac output. Apart from this, a smaller stroke volume, a slower maximal heart rate, a decreased arteriovenous oxygen difference, and muscle blood flow are other important mechanisms. 35, 45, 50 There are at least 2 potential limitations of our study that merit discussion. The first limitation is the crosssectional design because causal inferences cannot be concluded. Further, our study included only White individuals of European ancestry. In spite of these limitations, our study has some significant strengths. First, our well-characterized population-based cohort with a large number of individuals, including men and women, all socioeconomic strata, and a wide age range. Second, the standardized assessment of VO 2peak measured by CPET. One of the most relevant strengths are the 2 different imaging techniques we used. The cMRI data are especially important for the RV parameters because it is difficult to examine them in the echo, and cMRI images are much more meaningful. Finally, the availability of data on several metabolic risk factors, which were used as confounders. Data S1. The All participants underwent an extensive standardized medical examination. Anthropometric measurements included height and weight based on recommendations of the World Health Organization (WHO) 55 . Weight was measured to the nearest 0.1 kg in light clothing and without shoes using standard digital scales. BMI was calculated as weight (kg) / height² (m²). Waist circumference (WC) was assessed to the nearest 0.1 cm using an inelastic tape midway between the lower rib margin and the iliac crest in the horizontal plane. The subjects were standing comfortably with body weight evenly distributed between both feet. Waist-to-hip ratio was calculated as WC divided by height. FFM and fat mass (FM) were measured by bioelectrical impedance analysis (BIA) using a multifrequency Nutriguard-M device (Data Input, Pöcking, Germany) and the NUTRI4 software (Data Input, Pöcking, Germany) in participants without pacemakers. BP was assessed after a five-minute resting period in sitting position. Systolic and diastolic BP as well as heart rate were measured three times, with three minutes rest in between, on the right arm using a digital BP monitor (HEM-705CP, Omron Corporation, Tokyo, Japan). The mean of the second and third reading was used for the present analyses. Mean arterial pressure was calculated as (2/3) X diastolic BP + (1/3) X systolic BP. Antihypertensive medication was defined as use of agents with the anatomic, therapeutic, and chemical (ATC) code C02, C03, C07, C08 and C09. Hypertensive patients were identified by either self-reported antihypertensive medication or a systolic BP above 140 mmHg and/or a diastolic value more than 90 mmHg. Fasting (defined as at least 8 hours since the last meal) and non-fasting venous blood samples were obtained from all study participants in supine position between 7 am and 4 pm. Diabetes mellitus was defined as self-reported and/or glycated hemoglobin ≥ 6.5% and/or non-fasting glucose ≥ 11.1 mmol/l and/or current self-reported use of any hypoglycemic medication defined by the ATC code A10. Cardiac MR imaging was performed on a 1.5-T MR system (Magnetom Avanto; Siemens Medical Systems, Erlangen, Germany) as previously described 31 . Quantitative image analysis was performed by two observers with 3 and 5 years of cardiac MR imaging experience using semiautomatic tools in QMass MR 7.2 (MEDIS, Leiden, Netherlands). Interobserver variability was computed in a random subsample of 5%. Certification examinations for interobserver variations revealed an agreement of >90% 28 . Postcontrast images were interpreted of the two readers mentioned above and supervised in a consensus reading by a radiologist with 12 years of experience. All observers were unaware of the participants' medical history. For the RV measurements, RVEDV and RVESV were manually traced in enddiastole and end-systole in transverse axis view. Volumes below the pulmonary valve were included. At the inflow tract, thin-walled structures without trabeculations were not included as part of the RV. RVEDV was determined during the first image of the acquisition. RVESV was measured by determining the phase in which the RV intracavity blood pool was at its smallest by visual assessment at the midventricular level. To characterize the study sample, data is reported as the median (25 th and 75 th percentile) for continuous variables and as percentages for categorical variables stratified by quartiles and sex. The p for trend was calculated by univariate linear regression models with the continuous VO2peak variable as outcome and each of the listed variables as explanatory variables. The association of VO2peak with RV parameters was investigated by linear regression models adjusted for age, sex (not when stratified by sex), body fat mass, height 2.7 , systolic BP, use of antihypertensive medication, glycated hemoglobin, use of hypoglycemic medication, smoking status and eGFR. In order to evaluate the robustness of our findings in light of dropout from baseline to follow-up examination (SHIP-0 to SHIP-2) and individuals that did not take part in the echocardiographic and MRI examinations, we performed inverse probability weighting 32 , assuming a missing at random mechanism 33 , based on sociodemographic and health-related variables in our analyses. Inverse-probability weights were applied to consider drop-outs of individuals between SHIP-0 and SHIP-2 and between the basic and the CPET examinations. The intention behind these weights is to weight up the impact of individuals from groups, who are more likely to drop out of the study, and to weight down the impact of individuals from groups, who are less likely to drop out, in the regression analyses. To calculate these weights we used logistic regression models with participation at the CPET-examination as outcome and sociodemographic, behavioral, and cardiovascular risk factors from the core examinations as explanatory variables. For SHIP-2 participants, we additionally computed weights for the drop-out from SHIP-0 to SHIP-2 and multiplicatively combined these weights with the CPET-weights. With this approach, we aimed to improve the representativeness of our analyses. We used fractional polynomials to test potentially non-linear relationships between exposure and outcomes 34 The model assumptions were verified and confirmed. We tested for multicollinearity. There was collinearity between age and eGFR (-0.65), but we decided to keep our original adjustment because of the effects of kidney function on CRF and heart geometry and function as suggested by previous cardiovascular studies. On the other hand, the exclusion of eGFR did not lead to a major change in our results (please see below). With eGFR Adjusted for age, sex, body fat mass, systolic blood pressure, use of antihypertensive medication, glycated hemoglobin, use of hypoglycemic medication, smoking status and estimated glomerular filtration rate (CKD-EPI formula). Data was weighted according to dropout from baseline to follow-up examination (SHIP-0 to SHIP-2) and individuals that did not take part in the echocardiographic and MRI examinations (SHIP-2 and SHIP-Trend). RVEDDright ventricular end-diastolic diameter, RVOTright ventricular end-diastolic outflow tract diameter, MPAPmean pulmonary arterial pressure, TAPSEtricuspid annular plane systolic excursion, e´/a´ ratiolateral early and late tricuspid annular peak diastolic velocity ratio, RVEDVright ventricular end-diastolic volume, RVESVright ventricular end-systolic volume, RVSVright ventricular stroke volume, RVCO -right ventricular cardiac output, RVEFright ventricular ejection fraction UK biobank contributes to aerobic and muscle fitness research Cardiovascular disease in Europe: epidemiological update 2016 Inverse relationship of maximal exercise capacity to hospitalization secondary to coronavirus disease 2019 The obesity paradox in infections and implications for COVID-19 Epidemiology: Physical activity, exercise and mortality Obesity and heart failure: Epidemiology, pathophysiology, clinical manifestations, and management Exercise and the cardiovascular system: clinical science and cardiovascular outcomes Importance of assessing cardiorespiratory fitness in clinical practice: a case for fitness as a clinical vital sign: a scientific statement from the Role of inactivity in chronic diseases: evolutionary insight and pathophysiological mechanisms Effects of exercise training on left ventricular function in normal subjects: a longitudinal study by radionuclide angiography Physical activity and right ventricular structure and function Cardiorespiratory fitness, exercise, and blood pressure The athlete's heart: different training responses, gender and ethnicity dependencies Physical activity and physiological cardiac remodelling in a community setting: the Multi-Ethnic Study of Atherosclerosis (MESA) The heart of trained athletes: cardiac remodeling and the risks of sports, including sudden death van der Laarse A & van der Wall EE The athlete's heart The athlete's heart. 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Dr Ewert reports grants and personal fees from Janssen, personal fees from Bayer Vital, grants and personal fees from Boehringer Ingelheim, grants and personal fees from OMT, and personal fees from Berlin Chemie outside the submitted work. Dr Gläser reports personal fees from Boehringen Ingelheim, personal fees from Roche Pharma, and personal fees from Berlin Chemie outside the submitted work. The remaining authors have no disclosures to report. Data S1 Tables S1-S2 References 55-57