key: cord-0029871-wou3482s
authors: Miyachi, Yosuke; Kaido, Toshimi; Hirata, Masaaki; Sharshar, Mohamed; Macshut, Mahmoud; Yao, Siyuan; Kamo, Naoko; Kai, Shinichi; Yagi, Shintaro; Uemoto, Shinji
title: Intraoperative High Fraction of Inspiratory Oxygen is Independently Associated with Worse Outcome After Living-Donor Liver Transplantation: A Retrospective Study
date: 2022-04-13
journal: World J Surg
DOI: 10.1007/s00268-022-06544-7
sha: 872f879dc4405ebd71fb44cb17793a5fd6585100
doc_id: 29871
cord_uid: wou3482s

BACKGROUND: Ischemia and reperfusion injury is an important factor that determines graft function after liver transplantation, and oxygen plays a crucial role in this process. However, the relationship between the intraoperative high fraction of inspiratory oxygen (FiO(2)) and living-donor-liver-transplantation (LDLT) outcome remains unclear. PATIENTS AND METHODS: A total of 199 primary adult-to-adult LDLT cases in Kyoto University Hospital between January 2010 and December 2017 were enrolled in this study. The intraoperative FiO(2) was averaged using the total amount of intraoperative oxygen and air and defined as the calculated FiO(2) (cFiO(2)). The cutoff value of cFiO(2) was set at 0.5. RESULTS: Between the cFiO(2) <0.5 (n = 156) and ≥0.5 group (n = 43), preoperative recipients’ background, donor factors, and intraoperative parameters were almost comparable. Postoperatively, the cFiO(2) ≥0.5 group showed a higher early allograft dysfunction (EAD) rate (P = 0.049) and worse overall graft survival (P = 0.036) than the cFiO(2) <0.5 group. Although the cFiO(2) ≥0.5 was not an independent risk factor for EAD in multivariable analysis (OR 2.038, 95%CI 0.992–4.186, P = 0.053), it was an independent risk factor for overall graft survival after LDLT (HR 1.897, 95%CI 1.007–3.432, P = 0.048). CONCLUSION: The results of this study suggest that intraoperative high FiO(2) may be associated with worse graft survival after LDLT. Avoiding higher intraoperative FiO(2) may be beneficial for LDLT recipients. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00268-022-06544-7.

In liver transplantation, ischemia and reperfusion injury (IRI) is one of the important factors that determine postoperative graft function [1] . The abrupt cessation of blood flow and subsequent ischemia decreases several antioxidants, such as glutathione, and also tissue adenosine triphosphate [2] [3] [4] . This ischemic phase itself damages the liver tissue [4] ; moreover, after reperfusion, numerous cellular and molecular factors in various pathways are rapidly activated, which further aggravate the liver injury [1] . The ultimate consequence of this process is apoptotic or necrotic cell death, graft dysfunction, and graft loss [1] . From the findings of previous studies, it is now becoming clear that oxygen and its free radicals, called reactive oxygen species, play an important role in this process [5, 6] .

Oxygen therapy was once regarded as a harmless treatment option [7] ; however, several studies conducted in the field of critical care medicine have demonstrated that too much oxygenation could be harmful to the human body and that the restriction of oxygen supply would improve the outcomes of critically ill patients [8, 9] . Among patients admitted to the intensive care unit (ICU) following resuscitation of cardiac arrest, Kilgannon et al. showed that the first partial pressure of arterial oxygen (PaO 2 ) after ICU admission equal to or more than 300 mmHg was independently associated with increased in-hospital mortality [10] . Therefore, avoiding excessive oxygen supply is now becoming a new standard for patients' care [8] .

Based on these findings, we hypothesized that excessive oxygen supply before and after graft reperfusion may influence the severity of IRI and the outcome after liver transplantation; however, this topic still remains unexplored. From the above, the aim of this study was to investigate the impact of higher intraoperative fraction of inspiratory oxygen (FiO 2 ) on the outcome after livingdonor-liver-transplantation (LDLT).

A total of 219 adult (C18 years old) patients underwent primary LDLT in Kyoto University Hospital from January 2010 through December 2017. Among them, patients with posterior segment graft (n = 8), who died within seven days of LDLT (n = 2) and with preoperative pulmonary complications (hepato-pulmonary syndrome and moderate to severe pulmonary hypertension or intrapulmonary arteriovenous shunt, n = 7) were excluded. In addition, patients with incomplete operation records (n = 3) were also excluded. Ultimately, 199 patients were enrolled in this study.

The donor-recipient selection criteria, detailed surgical procedure, and regimens of postoperative immunosuppression, including blood type incompatible liver transplantation, have been described previously [11] [12] [13] . In our institute, the anesthesiology team is responsible for the intraoperative circulatory and respiratory care of patients. Although the target of intraoperative FiO 2 was set at approximately 0.4, the anesthesiologists were free to control the FiO 2 at their discretion.

All study protocols were approved by the Ethics Committee of Kyoto University (Approval number: R1473-4), and all procedures were conducted in accordance with the Declaration of Helsinki of 1996.

Data were retrospectively collected from patient charts. The preoperative recipient demographic data collected were age, sex, status prior to surgery (hospitalized or ICU stay), etiology of liver disease, Model for End-stage Liver Disease (MELD) score, hepatorenal syndrome, percent vital capacity (%VC), forced expiratory volume in one second/forced vital capacity (FEV1/FVC ratio), and results of blood sample tests. The donor demographic data collected were age, graft-to-recipient weight ratio (GRWR), blood type-incompatible donor, and graft type. Intraoperative parameters included operation time, blood loss, cold and warm ischemia time, portal vein pressure (PVP), intraoperative splenectomy and total volume of infusion. Postoperative parameters included in-hospital mortality, result of blood culture, acute cellular and humoral rejection within one year after LDLT, early allograft dysfunction (EAD) rate, and causes of graft loss. Postoperative platelet count until postoperative day (POD) 42 and bilirubin level until POD 7 were also collected.

The intraoperative FiO 2 usually fluctuated during the operation; therefore, to precisely assess the difference of intraoperative FiO 2 between patients, the value was averaged using the total amount of intraoperative oxygen (O 2 ) and air administered, i.e., the calculated FiO 2 (cFiO 2 ) was defined as {O 2 (L) ? Air(L) 9 0.21}/{O 2 (L) ? Air(L)}.

Recipients with %VC C80% and FEV1/FVC ratio C70% were classified as having normal pulmonary function. There were 23 cases of recipients (15 and 8 cases for cFiO 2 .5 and C0.5 group, respectively) whose preoperative pulmonary functions were not measured due to the patient's condition.

The results of arterial blood gas analyses including the PaO 2 , partial pressure of arterial carbon dioxide (PaCO 2 ), HCO 3 -, and PaO 2 /FiO 2 (P/F) ratio were obtained at three time points during operation: at the start, within 30 min before or after portal reperfusion, and at the end of the operation.

Recipients' preoperative skeletal muscle mass index (SMI), visceral to subcutaneous adipose tissue area ratio (VSR), and intra-muscular adipose tissue content (IMAC) were also collected, because our team previously reported that preoperative low SMI, high IMAC, and high VSR, i.e., ''positive 3 body composition markers,'' are independent risk factors for mortality after LDLT [14] .

EAD was defined by the presence of one or more of the following: (i) total bilirubin C10 mg/dL on POD 7, (ii) prothrombin time-international normalized ratio C1.6 on POD 7, and (iii) aspartate aminotransferase or alanine aminotransferase C2000 IU/mL within the first seven postoperative days [15] .

First, the distribution of cFiO 2 was evaluated and the factors associated with the difference of cFiO 2 between patients were assessed. Second, based on the previous study [16] , patients were divided according to the cutoff value of the cFiO 2 ''0.5.'' Subsequently, the background and postoperative outcomes of patients were evaluated. Third, the prognostic factors associated with EAD were evaluated using univariable and multivariable analyses. The data included in the univariable analysis were determined according to the previous reports [17, 18] . Although the previous study has shown that a high preoperative bilirubin level is a risk factor for EAD [17] , in this analysis, the clearance of total bilirubin level during the first seven days after LDLT, defined as ''(preoperative bilirubin-POD7 bilirubin)/preoperative bilirubin,'' was used instead of a simple preoperative bilirubin level. Finally, the postoperative survival curves of recipients with the cFiO 2 .5 and C0.5 were compared. Subsequently, the prognostic factors associated with overall graft survival were analyzed using univariable and multivariable analyses. The data included in the univariable analysis were also determined according to the previous reports [14, [19] [20] [21] [22] [23] .

Continuous data were expressed as the median and interquartile range (IQR) and were compared using the Wilcoxon rank-sum test. Categorical data were expressed as counts, and percentages and were compared using the Fisher's exact test. The overall graft survival rate was calculated using the Kaplan-Meier method, and differences between curves were evaluated using the log-rank test. Variables with P .10 in the univariable analysis were considered candidates for multivariable logistic regression analysis or Cox regression analysis. The results of the multivariable analysis are shown as odds ratios (ORs) or hazard ratios (HRs) with 95% confidence intervals (CIs). According to the previous recommendation of the American Statistical Association [24] , we avoided describing P .05 as ''statistically significant''; instead, we described the P values as continuous quantities in the text, figures, and tables. All statistical analyses were performed using JMP Pro, version 14.0.0 (SAS Institute, Inc., Cary, NC). The cFiO 2 showed a bimodal distribution, and the peaks were found around 0.4 and 0.5 ( Fig. 1) ; therefore, we evaluated the factors associated with the difference of the cFiO 2 between patients ( Fig. 2) . First, we divided the patients according to the P/F ratio at the start of the operation ( Fig. 2A and B) ; however, both the P/F ratio C300 and C400 groups had a peak around cFiO 2 0.5 and the P/F ratio \400 group also showed a peak around cFiO 2 0.4. Patients were also divided according to their pulmonary function (Fig. 2C ), preoperative status (Fig. 2D ), MELD score (Fig. 2E) , and PaO 2 at the start of the operation (Fig. 2F) ; however, none seemed to influence the variance of cFiO 2 . Finally, we also assessed the trend of the cFiO 2 during the observational period. As shown in Fig. 3 , no apparent chronological changes in cFiO 2 were found during this observational period. (Table 1) Recipients were divided into those with the cFiO 2 .5 (n = 156) and C0.5 (n = 43). Both groups showed almost comparable preoperative recipients' background, donor factors, and intraoperative parameters. Although the P values of recipients' preoperative alanine aminotransferase and intraoperative warm ischemia time were less than 0.05, the difference in actual values was almost clinically negligible. Postoperatively, the cFiO 2 C0.5 group showed a trend toward higher EAD rate than the cFiO 2 .5 group. Figure 4 shows the results of arterial blood gas analyses during the operation. PaO 2 was first comparable between the two groups; however, the cFiO 2 C0.5 group showed higher PaO 2 during portal reperfusion and at the end of the operation compared to the cFiO 2 .5 group (Fig. 4A ). The levels of PaCO 2 and HCO 3 were almost similar between the two groups throughout the operation ( Fig. 4B and C). The P/F ratio was first lower in the cFiO 2 C0.5 group; however, the values became almost comparable thereafter (Fig. 4D) .

Comparisons of the postoperative platelet count and total bilirubin values are shown in Fig. 5 . The cFiO 2 C0.5 group showed a trend toward lower platelet count after POD5 (Fig. 5A ) and higher bilirubin levels after POD3 (Fig. 5B) .

b Fig. 2 Evaluation of the factors associated with the variance of the cFiO 2 . The distribution of cFiO 2 was divided according to the P/F ratio (Cor \300 and 400, A and B, respectively), recipients' pulmonary function (C), recipients' preoperative status (D), MELD score (E), and PaO 2 at the start of the operation (F). The normal pulmonary function was defined as recipients with %VC C80% and FEV1/FVC ratio C70%. Calculated FiO 2 (cFiO 2 ) was defined as Fig. 4 The results of arterial blood gas analysis during operation. The cFiO 2 C0.5 group showed higher PaO 2 at the portal reperfusion and at the end of the operation compared to the cFiO 2 .5 group (A). The results of PaCO 2 (B) and HCO 3 -(C) were almost similar between the two groups throughout the operation. The P/F ratio was initially lower in the cFiO 2 C0.5 group; however, the value became almost comparable between the two groups during portal reperfusion (D). Calculated FiO 2 (cFiO 2 ) was defined as {O 2 (L) ? Air(L) 9 0.21}/{O 2 (L) ? Air(L)}. FiO 2 , fraction of inspiratory oxygen; PaCO2, partial pressure of arterial carbon dioxide; PaO 2 , partial pressure of arterial oxygen; P/F, PaO 2 / FiO 2 ratio World J Surg

Univariable analysis revealed that recipient age, gender, donor age, left lobe graft, and the cFiO 2 C0.5 were potential risk factors for EAD after LDLT (Table 2 ). Multivariable analysis showed that the male recipients (P = 0.048) and donor age (P = 0.023) were independent risk factors for EAD. Although the P value did not reach the statistical threshold, the cFiO 2 C0.5 was considered as a possible risk factor for EAD (P = 0.053). Figure 6 shows the overall graft survival after LDLT. The cFiO 2 C0.5 group showed worse graft survival than the cFiO 2 .5 group. Table 3 shows the results of the risk factor analysis for overall graft survival after LDLT. Univariable analysis revealed that the positive 3 body composition markers, donor age C40 years, GRWR .6% blood type incompatible donor, and the cFiO 2 C0.5 were potential risk factors for graft loss after LDLT. Multivariable analysis showed that the positive 3 body composition 

The results of this study demonstrated that high intraoperative FiO 2 may be independently associated with worth graft outcome after LDLT. This is also confirmed by the fact that the cFiO 2 C0.5 group showed higher EAD rate, although the cFiO 2 C0.5 was not shown to be an independent factor in multivariable analysis.

In a previous experimental study, it was shown that perioperative hyperoxic conditions worsen liver IRI. Using a mouse partial liver ischemia-reperfusion model, Zangl et al. demonstrated that the mice under postoperative hyperoxic (FiO 2 = 0.6) conditions showed higher glutamate-pyruvate-transaminase level, reactive oxygen species, and histological injury score than the mice under normoxic (FiO 2 = 0.21) conditions [25] . Subsequently, they showed that the harmful effects of high FiO 2 were ameliorated by depletion of granulocytes or Kupffer cells or by knocking out of the p47phox unit of the NADPHoxidase [25] . Considering that granulocytes and Kupffer cells are the main sources of reactive oxygen species, their results suggested that oxygen is a very important factor that affects IRI and that restriction of oxygen supply may decrease the severity of liver injury after ischemiareperfusion.

In our study, the cFiO 2 C0.5 groups showed a worse overall graft survival after LDLT. Regarding short-term outcomes, the cFiO 2 C0.5 group was also associated with a higher rate of EAD. Univariable analysis showed that higher cFiO 2 was a potential risk factor for EAD; however, it did not reach the statistical threshold for independent risk The factor is included in the analysis as continuous data. Calculated FiO 2 (cFiO 2 ) was defined as {O 2 (L) ? Air(L) 9 0.21}/{O 2 (L) ? Air(L)}. P .05 on multivariate analysis. àThe formula for total bilirubin clearance is as follows: (preoperative bilirubin-POD7 bilirubin)/preoperative bilirubin ALT alanine aminotransferase; AST aspartate aminotransferase; CI confidence interval; EAD early allograft dysfunction; FiO 2 fraction of inspiratory oxygen; GRWR graft-to-recipient weight ratio; OR odds ratio; P/F PaO 2 /FiO 2 ratio; PVP portal vein pressure factor in multivariable analysis. This may be because the difference in cFiO 2 between the two groups (median 0.44 for the cFiO 2 .5 group and 0.53 for the cFiO 2 C0.5 group, respectively) was not strong enough to detect the difference in the EAD rate. If we could have compared the FiO 2 0.3 and 0.8, which were used in several prospective studies [26] [27] [28] , the difference in short-term outcomes might have appeared more clearly. Liver transplantation is a life-saving procedure, and recipients are usually critically ill; therefore, it would be ethically difficult to conduct a prospective study allocating patients into extremely different intraoperative FiO 2 . We think the results of this study suggested the possibility that high intraoperative FiO 2 might increase the EAD rate and could lead to worse graft outcomes after LDLT. A lower P/F ratio at the start of the operation would be the main reason for the higher oxygen supply in the cFiO 2 C0.5 group (Fig. 4D) . However, in the cFiO 2 C0.5 group, the P/F ratio improved thereafter and the PaO 2 increased during portal reperfusion than in the cFiO 2 .5 group (Fig. 4A ). Resolving of atelectasis by positive pressure ventilation or removal of a large amount of ascites or pleural effusion may be associated with improved oxygenation in the cFiO 2 C0.5 group. As shown in the Supplementary Figure, spot FiO 2 was gradually decreased during operation even in the cFiO 2 C0.5 group; however, the difference was smaller than that observed in the cFiO 2 .5 group; therefore, the FiO 2 became relatively higher than expected, and the liver grafts were exposed to a higher oxygen environment in the cFiO 2 C0.5 group. These results suggest that intraoperative FiO 2 can be reduced safely in some patients, which may lead to the suppression of IRI, lower EAD rate, and improved outcome after LDLT. Our study has several limitations. First, the study was retrospective and conducted in a single institution. Our results should be confirmed in multicenter prospective studies. Second, it would have been more informative if we could have assessed all possible parameters in our institute during risk factor analysis for EAD and graft survival. However, due to the limited number of patients, we selected the factors included in the analysis, according to the results of previous studies. Further accumulation of cases is needed for a more accurate investigation. Last, we have to be careful about applying the results of this study to the patients undergoing LDLT. We think that these results do not mean the intraoperative FiO 2 should be kept ''lower as possible.'' Intraoperative FiO 2 should be adjusted according to the condition of each patient and critically ill patients sometimes need higher than usual oxygen to maintain a normal oxygenation level. However, as is shown in this study, even the most of the patients in cFiO 2 .5 group showed PaO 2 over 120 mmHg and those in cFiO 2 [0.5 group showed further higher PaO 2 during LDLT; therefore, we think that FiO 2 could be reduced safely for these patients. From the above, we think that our results should be interpreted as ''avoiding unnecessarily high intraoperative FiO 2 in LDLT.''

In conclusion, this retrospective study suggested that intraoperative high FiO 2 might increase EAD and have some impact on graft survival after LDLT. Avoiding unnecessarily high intraoperative oxygenation may have some beneficial effects in patients undergoing LDLT.

The online version contains supplementary material available at https://doi.org/10.1007/s00268-022-06544-7.

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Acknowledgements None.Author Contributions YM participated in research design, paper writing, and data analysis. TK revised the draft critically for important intellectual content. MH made substantial contributions to the design of the work and analyzed data. MS made substantial contributions to the design of the work and analyzed data. MM made substantial contributions to the design of the work and analyzed data. SY made substantial contributions to the design of the work and analyzed data. NK made substantial contributions to the design of the work and analyzed data. SK made substantial contributions to the design of the work. SY revised the draft critically for important intellectual content. SU made substantial contributions to the design of the work and gave final approval of the version to be published.Funding None.

Conflict of interest All authors declared no conflict of interest.Informed Consent Informed consent was obtained from all individual participants included in the study.Ethical Approval All study protocols were approved by the Ethics Committee of Kyoto University (Approval number: R1473-4), and all procedures were conducted in accordance with the Declaration of Helsinki of 1996.