key: cord-0040371-p2ahklyl
authors: Khabbaz, Rima; Bell, Beth P.; Schuchat, Anne; Ostroff, Stephen M.; Moseley, Robin; Levitt, Alexandra; Hughes, James M.
title: Emerging and Reemerging Infectious Disease Threats
date: 2014-10-31
journal: Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases
DOI: 10.1016/b978-1-4557-4801-3.00014-x
sha: ffae196fecc4da7f1897e22f8ce0dc0e03c8bcec
doc_id: 40371
cord_uid: p2ahklyl

nan

Throughout history, infectious diseases have been inextricably linked with human health, affecting the development and advancement of societies as well as human evolution. 1, 2 Those linkages remain well defined today, as infectious pathogens find new ways to exploit human vulnerabilities and elude control efforts across a highly connected world. Widespread movement of people, animals, and goods, exploding population numbers, urban development, environmental degradation, centralized food production, and other contributing factors (Table 14-1) have given microbes rapid and easy access to new populations and geographic areas and spawned a host of emerging and reemerging infectious diseases-the majority of which are zoonotic (Table 14 -2). 3 With their remarkable adaptability, emerging infections can spread quickly and gain strongholds to become endemic diseases, as most profoundly demonstrated by the decades-long pandemic of human immunodeficiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS).

Data from the Global Burden of Disease Study 2010 (GBD 2010), a landmark collaboration of 486 scientists from 302 institutions across 50 countries, 4 indicate that nearly one fourth of the estimated 52.8 million deaths that occurred in 2010 were associated with infectious diseases. 5 From 1990 to 2010, overall deaths from communicable diseases declined, with significant decreases in mortality from lower respiratory tract infections (from 3.4 to 2.8 million) and diarrheal diseases (from 2.5 to 1.4 million). 5 However, infectious diseases remained leading killers, especially among young children ( Fig. 14-1 ) and accounted for large burdens of disability-adjusted life years worldwide. 5, 6 Although deaths from HIV infection/AIDS peaked in 2006 and have since showed steady declines owing to fewer new infections and increased availability of antiretroviral therapy and care, HIV infection/ AIDS remains a leading cause of disease burden and death-responsible for an estimated 1.5 million deaths in 2010. 5 Tuberculosis and malaria also continue to exact a tremendous toll, each causing approximately 1.2 million deaths in 2010. 5 The multifactorial impact of infectious diseases is most prominent in low-income countries, with infectious diseases causing severe morbidity, impeding economic development, and compromising political stability.

Beyond infectious diseases, microbial agents have been identified as the cause or contributing factor in a number of chronic diseases (Table 14-3 ). In 2008, approximately 2 million new cancer cases were linked to infections. 7 Among the leading causes of cancer-related deaths, three are caused by infectious agents: hepatocellular carcinoma by hepatitis B and C viruses, cervical cancer by human papillomavirus, and gastric cancer by Helicobacter pylori bacteria. Many other potential infectious/chronic disease linkages are also being explored, including Chlamydia pneumoniae and multiple sclerosis, 8, 9 Alzheimer's disease, 9 and atherosclerosis 10 ; enteroviruses and type 1 diabetes 11, 12 ; and rhinoviruses and childhood asthma. 13 In addition, several genetic factors have been shown to influence infectious disease susceptibility and disease progression (Table and hundreds of others are under investigation. 14 The past few decades have provided numerous examples of the ongoing threat of infectious diseases and the ability of microbes to evolve, adapt, and survive. In particular, acute respiratory viruses are often among the most recognized emerging and reemerging infectious diseases because of the high disease burden they produce. Examples in the past decade include a novel coronavirus that resulted in the 2003 global outbreak of severe acute respiratory syndrome (SARS), 15 17 ; and avian influenza A H7N9 in China in 2013. 18 Emerging and reemerging vector-borne infections also remain priorities, as mosquito-borne viruses such as dengue and chikungunya continue to appear in new areas and tick-borne diseases continue their steady rise. In addition, increased attention is being given to environmental fungi as a cause of human and animal infections. Recent examples include the emergence of Cryptococcus gattii infections in the U.S. Pacific Northwest, 19, 20 increasing numbers of Coccidioides infections, which are a major cause of community-acquired pneumonia in California and the southwest United States, 21 and novel fungal infections as a cause of health care-associated infections (HAIs). 22 International trade and travel along with globally mobile populations present particular challenges for controlling infectious diseases, highlighting concerns for spread of known infections such as tuberculosis 23 and vaccine-preventable diseases 24 along with introduction of new threats. 25 In 2011, international tourist arrivals approached 1 billion worldwide, and they are expected to nearly double over the coming decades ( Fig. 14-2 ). 26 Moreover, today's globalized food supply has resulted in an increasing number of foodborne illnesses, many of which have severe consequences-especially for more vulnerable populations such as children, immunocompromised individuals, and the elderly. Also on a global level is the growing problem of resistance to antimicrobial agents, which continues to impede treatment and control efforts for an increasing number of pathogens.

The development of safe, effective vaccines coupled with large-scale immunization programs represents the ultimate solution to infectious diseases. 27 Routine childhood immunization in the United States prevents approximately 20 million illnesses and 42,000 premature deaths, while saving nearly $70 billion in direct and societal costs for each birth cohort vaccinated. 28 Although vaccination led to the eradication of smallpox in 1980 and has brought the world closer than ever to eradication of poliomyelitis, vaccine-preventable diseases can reemerge even in the setting of high-functioning immunization programs. A variety of factors contribute to outbreaks of vaccine-preventable diseases in the vaccination era (Table . [29] [30] [31] [32] Recently, countries in the Americas and Europe have confronted the reemergence of measles, mumps, and pertussis owing to diverse underlying causes. [33] [34] [35] Most resurgences of vaccine-preventable diseases stem from low immunization coverage. In many parts of the world, weak primary health care systems and limited access to the most vulnerable populations result in many children being unimmunized. More recently, reduced vaccine acceptance in several affluent countries has emerged as a threat to protecting communities from vaccine-preventable diseases. Whereas in the United States less than 1% of children receive no vaccines at all, 36 some parents refuse one or more vaccines or decide to delay or increase intervals between vaccinations.

Two doses of measles-containing vaccines provide 95% vaccine efficacy, but at least 94% of the population must be vaccinated to ensure herd immunity, or population protection. Infants too young to be immunized and people who are immune compromised depend on herd immunity for protection. Accumulation of susceptible people in a community can result in periodic outbreaks when measles virus is circulating. 30 Endemic transmission of measles has been interrupted in the United States since 2000, 37 but travelers to areas where the virus still circulates account for 50 to 100 importations to the United States most years. Aggressive public health investigation of each suspected measles case is conducted to limit spread of the virus. Recently, several measles outbreaks in the United States have been associated with transmission among people who have not been vaccinated, intentionally, due to personal beliefs. In 2011, 222 measles cases occurred in the United States, the largest number of annual cases reported since 1996. 38 The most important source of imported virus in 2011 was Europe, where large outbreaks affected multiple countries. A very large outbreak in France (where an estimated 20,000 cases occurred) 39 was linked with importations to multiple countries in the Americas. 38, 40 Public health response to each outbreak is expensive. 24 Because measles is rarely seen in the United States, missed diagnosis can lead to sustained exposures particularly in health care settings (Fig. 14-3 ). 41 year-primarily in developing countries. 42 Enormous progress in measles immunization through supplemental immunization activities and increasing routine coverage, particularly in Africa, has led to a 74% reduction in worldwide measles deaths. 43 Despite this progress, the weak underlying immunization systems, famine, limited access associated with political disruption, and delayed emergency campaigns explain resurgences of measles in the Horn of Africa and southern Africa. 44 Low first-dose measles coverage and long intervals between campaigns offering second-dose opportunities allow rapid accumulation of children susceptible to measles and can lead to large outbreaks. Poor vaccine acceptance in selected communities, ranging from the Roma communities 45 to anthroposophists in Switzerland has led to sizable outbreaks in Europe as well. 46 34, 51 These outbreaks were difficult to control, and although the highest risk occurred among unvaccinated persons, many case-patients had received one or two doses of mumpscontaining vaccine, consistent with only 80% to 85% vaccine efficacy for one-and two-dose regimens. 48, 50 Although factors associated with disease include intense crowding, there is no evidence to support immune escape. 54 Recent outbreaks have been notable for very little transmission to the broader community. Although some proponents exist, the value of administering a third dose of mumps-containing vaccine in outbreaks that occur despite high two-dose coverage has been unclear because disease tends to be decreasing by the time additional doses are provided. 55 Because most mumps outbreaks have occurred in unusual settings where there is extreme crowding or extensive personal contacts and most mumps is self-limited and relatively mild, it is unlikely major investments will be made to attempt to improve the efficacy of mumps vaccines.

Despite decades of vaccination with first the whole-cell pertussis vaccine and, since the 1990s, acellular pertussis vaccine, pertussis is currently the least well-controlled vaccine-preventable disease in the United States. Cyclical increases are typical for pertussis, but since the 1990s the United States has experienced a continuing rise in incidence that exceeds changes due to better laboratory diagnostics or disease reporting ( Fig. 14-4 ). The highest rates of disease, hospitalizations, and fatalities occur among infants younger than 1 year of life. 56 Reported cases in teens and older children have been increasing since 2010. 57 It has become increasingly clear that the transition from whole-cell 1922 1930 1940 1950 1960 1970 1980 1990 2000 2010 0 Year pertussis virus vaccine "backbone" to acellular vaccines has changed the epidemiology of pertussis.

In 2010, California reported the highest rates of pertussis in 50 years. A retrospective analysis conducted there revealed vaccine efficacy was more than 95% within 3 years of receiving the last dose of acellular pertussis but decreased substantially by 5 years since vaccination. 35, 58 This explained the predominance of 7-to 10-year olds in the 2010 resurgence. By 2012, high rates of disease occurred in Washington State and several other areas, 57 with surprising increases also noted among 11-and 13-year olds. Questions have been raised about whether there is differential performance of Tdap booster vaccinations among teenagers who had received acellular vaccines as young children compared with the earlier experience of teenagers who received Tdap after earlier exposure to whole-cell vaccines. Although some have speculated that bacterial strain changes noted recently might account for disease resurgence, 59 the dominant view is that acellular vaccines provide good short-term protection but provide immunity that wanes more rapidly than initially expected. Program priorities in this context are to target reduction of deaths, which mainly affect infants in the first few months of life. Hence, recent recommendations for women to be vaccinated during every pregnancy are a priority for disease control. 60 

Infections involving the respiratory tract represent one of the most dynamic areas for emerging and reemerging diseases, producing dramatic examples such as the first recognized outbreaks of legionellosis in the 1970s and hantavirus pulmonary syndrome in the 1990s. Examples in the 21st century include newly recognized pathogens such as human metapneumovirus (first identified in 2001), 61 16 and a Middle Eastern novel coronavirus associated with severe respiratory illness identified in 2012. 17 Other newly recognized respiratory viruses include two additional coronaviruses (NL63 and HKU1), novel human polyomaviruses KI and WU, rhinovirus groups C and D, and parechoviruses, although in some instances the role of these agents as pathogens is still being clarified. 63 More virulent strains of known respiratory pathogens have also emerged. Examples of this phenomenon include human disease associated with avian influenza viruses (especially highly pathogenic avian influenza A [H5N1] and avian influenza H7N9 18,64 ), severe disease due to adenovirus type 14, 65 and extensively drug-resistant tuberculosis. 66 Acute respiratory tract infection constitutes a broad category of diseases that include infections of both the upper and lower respiratory tracts, such as acute pharyngitis, epiglottitis, bronchitis, pneumonia, and influenza. Although upper respiratory tract infections are capable of causing severe illness, virtually all (98%) respiratory disease-related deaths are a consequence of infection of the lower respiratory tract, especially pneumonia. Acute respiratory infections remain the leading cause of mortality from infectious diseases in the United States and around the world. The World Health Organization (WHO) estimates that 4.2 million deaths resulted from lower respiratory tract infections in 2004, accounting for 7.1% of all deaths that year. 67 Among persons who died of lower respiratory tract infections, 70% (2.9 million) were residents of low-income countries. Lower respiratory tract infections are the number one cause of death in the developing world. Even in high income countries, lower respiratory tract infections are the fourth leading cause of death, responsible for 4% of all-cause mortality. Mortality from lower respiratory tract infections has the greatest impact on young children. On a global basis, 42% of all deaths from lower respiratory tract infections (mostly due to pneumonia) occur in children younger than 5 years of age. 67 Significant influenza-and pneumoniaassociated mortality also occurs in persons older than 65 years of age and in individuals with chronic underlying pulmonary disease. In the United States, influenza and pneumonia are the leading cause of infectious disease-related mortality and the eighth most frequent cause of death. 68 

In 2001, human metapneumovirus (hMPV) was first reported as a cause of acute respiratory tract infections when the virus was isolated infection. 70, 81 Ribavirin and immune globulin have been used to treat severe disease but have not been systematically assessed, and efforts are underway to develop candidate vaccines against hMPV. 69, 82 Genetic analyses have demonstrated two major hMPV types, designated groups A and B, with two subtypes (1 and 2) within each group; subtype variants have also been reported. 69 The geographic distribution of groups and subtypes changes over time, and multiple variants can cocirculate. Periodic shifts in predominant circulating strains are thought to coincide with upsurges in disease incidence and severity. In some studies, group A viruses have been associated with more severe disease compared with group B viruses, although other series have found the opposite result or have not suggested a difference in disease severity between the two groups.

Although coronaviruses were once considered to be pathogens most typically associated with the common cold, their public health significance has changed considerably in recent years. 83 The 2003 outbreak of SARS is widely considered to be the most consequential emerging infectious disease event of the early 21st century, with profound public health, economic, sociologic, and political ramifications. 84 The expanded research and monitoring of coronaviruses that resulted from the SARS episode led to the recognition of two additional human coronaviruses (NL63 and HKU1) associated with upper respiratory tract infections. [83] [84] [85] [86] Most recently, a novel coronavirus was identified in 2012 among patients in the Middle East or patients linked to the Middle East. 17 This new virus, which is distinct from the SARS coronavirus, is also associated with severe and fatal pulmonary disease. A number of studies suggest that the human coronaviruses appear to be zoonotic in origin, with bats being especially important reservoirs. 87 Because bats harbor many coronaviruses, there is the potential for future pathogenic coronaviruses to emerge from this reservoir.

SARS was recognized in February 2003 when an explosive outbreak of adult respiratory distress syndrome was carried globally by more than a dozen individuals who were all guests at a Hong Kong hotel over a single weekend while a physician from adjacent Guangdong Province with fatal respiratory illness was also present. [88] [89] [90] Before traveling to Hong Kong, the source physician had been caring for patients with a similar illness, with 305 cases of undiagnosed respiratory diseases occurring in Guangdong Province since the previous November. A global consortium of laboratories rapidly identified the causative agent as a previously unrecognized betacoronavirus (SARS coronavirus [SARS-CoV]). 15 The outbreak was contained within 4 months of recognition, primarily through the employment of public health measures such as community isolation and quarantine. [88] [89] [90] In the interim, a total of 8,096 cases of SARS were recorded in 29 countries on five continents, with 774 (9.6%) fatalities. 91 However, 98% of the cases occurred in just five locations (Table 14-6); only 8 cases were confirmed in the United States. The following winter, four symptomatic and one asymptomatic community-acquired SARS cases occurred in Guangzhou. There was also a series of laboratory acquired infections in Taiwan, Singapore, and mainland China over the same time period. 88 No cases of SARS have been recognized anywhere in the world since 2004.

from specimens collected over a 20-year period from hospitalized children in the Netherlands with undiagnosed upper and lower respiratory tract illnesses. 61 Subsequent serologic and virologic analyses of banked specimens have demonstrated evidence of hMPV infection as far back as the 1950s, and genetic studies suggest the virus is considerably older. 61, 69 A paramyxovirus, hMPV is closely related to respiratory syncytial virus (RSV), another member of the Paramyxoviridae subfamily of Paramyxoviridae. 69, 70 These related viruses have many clinical and epidemiologic features in common, and coinfections with these two pathogens have even been described. 71 Studies examining whether hMPV and RSV coinfections produce more severe illness have shown conflicting results. 71 Since hMPV was first identified, major strides have been made in our understanding of the impact of this agent, which appears similar to other major viral respiratory pathogens. It is known to occur globally in both high-and low-income countries, and by 5 years of age virtually all children demonstrate evidence of prior hMPV infection. 72 Clinically significant illness and severe disease most commonly occur in children younger than 2 years of age, but hMPV-related illness can occur throughout childhood. 73, 74 In most studies, this virus (either alone or with copathogens) has been identified in 2% to 20% of children with acute respiratory tract infections, and in 3% to 7% of children hospitalized with acute respiratory tract infections or fever. 69, 73 Variation in rates of hMPV detection is likely due to patient selection criteria, sample collection, and diagnostic test methods. In contrast, hMPV is rarely (<1%) identified in children or adults who are not ill and when found is usually present in much lower titers than when detected during illness. 69, 73, 75 A recent multiyear, multicenter prospective study suggests that hMPV produces 20,000 hospitalizations, 263,000 emergency department visits, and 1 million outpatient visits annually among children younger than 5 years of age in the United States. 75 Because disease is also found in older children and in adults, these numbers represent an unknown proportion of the overall burden of illness due to hMPV. Multiyear studies of hMPV infection show that incidence varies from year to year and by season, with most infections in temperate locations occurring in winter and early spring. 70 In children, most severe hMPV disease manifests as pneumonia or bronchiolitis and a substantial proportion of hospitalized children have underlying medical conditions, particularly asthma. [73] [74] [75] [76] Intensive care can be necessary and fatal illnesses have occurred in children with hMPV infections. 77 Human metapneumovirus has also been associated with childhood upper respiratory tract infections (up to 10% in some series) as well as acute otitis media. 70 Most infections with hMPV in adults are mild, but severe disease can occur, especially in the elderly and in persons with chronic pulmonary disease or congestive heart failure. 78, 79 This virus has been estimated to involve 3% to 7% of acute respiratory tract infections and 4.5% of acute respiratory tract infection hospitalizations in adults. 79 Outbreaks associated with hMPV have been reported in both children and adults, especially among long-term institutionalized elderly, with case-fatality rates as high as 33%. 79, 80 As with RSV, hMPV can produce severe and recurrent disease in immunocompromised hosts, including transplant recipients, those with hematologic malignancies, and HIV 

No disease is more closely intertwined with the subject of emerging and reemerging infectious diseases than influenza. 108, 109 Arguably the most dramatic example of infectious disease emergence is the 1918-1919 Spanish influenza A H1N1 pandemic. This virus swept across the globe in successive waves with profound societal disruption in the midst of World War I, leaving in its wake an estimated number of fatalities ranging as high as 50 to 100 million people. 109 In the United States alone, an estimated 675,000 deaths occurred, increasing the overall mortality rate for that period by almost 40%. 109, 110 Two less consequential pandemics (caused by influenza A H2N2 in 1957 and influenza A H3N2 in 1968), but with excess mortality, also were observed in the 20th century. Influenza has proven to be even more volatile in the 21st century. Traditional dogma regarding which influenza A hemagglutinin subtypes (H1, H2, and H3) are responsible for illness in humans has been reconsidered based on recent events. These include cases of severe human disease due to highly pathogenic avian influenza A H5N1 that have been occurring since 2003 and the increasing recognition of human disease caused by other viruses (H7, H9, H10) usually considered avian subtypes. [111] [112] [113] As the attention of the public health community was focused on the pandemic potential of these avian subtypes, in 2009 another influenza virus (a triple reassortant A H1N1) suddenly appeared in North America to produce the first pandemic of the 21st century. 114 In the aftermath of the H1N1 pandemic, a variant strain of influenza A H3N2 that produces human disease in association with swine contact, mostly at agricultural fairs, has been recognized in the United States. 115 Further complicating the picture, in early 2013 an outbreak of influenza due to avian influenza A (H7N9) virus, not known to have previously infected humans, was reported across a wide area of eastern China. 18 The novel H7N9 virus is associated with unusually high mortality in humans but is a low pathogenicity variant in poultry. 116 These examples highlight the unpredictable nature of influenza and the critical need to maintain a high state of vigilance for this disease through global surveillance in people and animals along with research to develop improved treatment, prevention, and control measures.

Human disease due to highly pathogenic avian influenza (HPAI) H5N1 was first recognized when a fatal illness occurred in a 3-year old child in Hong Kong in the spring of 1997. 117 "Highly pathogenic" is the designation used to describe avian influenza viruses with certain genetic traits that produce lethal disease outbreaks in poultry. This case was followed later in the year by a series of 18 human illnesses (6 fatal) in children and young adults that led to the mass culling of poultry in Hong Kong as a measure to control bird-to-human transmission. 118 The virus responsible for this outbreak is referred to as A/Goose/ Guangdong/1/96 H5N1 because it was first identified in a goose in southern China the previous year. All subsequent human H5N1 illnesses, as well as the animal outbreaks that have led to the natural death and culling of more than 250 million poultry and wild birds, have been caused by descendants of the 1996 goose Guangdong lineage. 119, 120 This virus has evolved into two major clades (1 and 2) and numerous additional clades and subtypes that have spread over three continents during sequential waves believed to originate in southern China. 119, 121 After the initial 1997 Hong Kong outbreak, episodes of human illness due to avian influenza subtypes (especially H7 and H9) were identified in Europe, Asia, and North America, producing both respiratory disease and conjunctivitis. [122] [123] [124] In early 2003, a father and son from Hong Kong who had traveled to southern China were diagnosed with H5N1 influenza at the time that the outbreak that became SARS was recognized, briefly raising concern this may have been the etiology of the outbreak. 125 However, it was not until late 2003, when SARS had subsided, that the current outbreak of H5N1 human illnesses began when cases were recognized in Vietnam and Thailand. 126, 127 In contrast to previous episodes, these cases were more widely dispersed and there was evidence of widespread circulation of HPAI H5N1 in poultry, waterfowl, and wild birds in the affected areas.

Several features of SARS are especially noteworthy. These include (1) a relatively long incubation period (mean 4.6 days, range 2 to 14 days); (2) minimal transmissibility early in the course of illness (viral shedding in respiratory secretions followed a crescendo-decrescendo pattern with peak titers on day 10 of illness); (3) a high proportion of illnesses (21%) in health care workers; (4) a low-to-moderate basic reproductive number (R0), estimated at 2 to 4 secondary cases per average infectious case 92 ; (5) the occurrence of "super-spreader" events facilitated by inpatient therapeutic procedures that promoted aerosolization of the virus and accounted for much of the observed personto-person transmission (the majority of cases resulted in no secondary transmission); (6) a rarity of asymptomatic infections; and (7) a striking age-dependent case-fatality ratio (<1% in persons <24 years of age vs. >50% in those >65 years). [88] [89] [90] Epidemiologic investigations found that people with early SARS cases in Guangdong Province often had contact with exotic live animal markets. Several species (especially palm civet cats) in the live markets had evidence of SARS-CoV infection, leading to mass culling as a control measure. 88 The virus was not detected in these animals in the wild, and experimental infections showed the animals developed SARS-like symptoms. Both factors suggested they were not the natural host. In 2005, viruses similar to SARS-CoV (SARS-like coronaviruses [SL-CoV]) were identified in horseshoe bats in southern China and bats are now considered to represent the natural reservoir. 87,93-95 Subsequent studies have shown bats in many parts of the world harbor SL-CoVs. 87, 95 Various therapies were employed during the SARS outbreak, including ribavirin, corticosteroids, antiretroviral drugs, and immunotherapy. Some appeared beneficial, but none could be systematically evaluated. 89 SARS-CoV is known to act through a spike protein that targets angiotensin-converting enzyme 2, offering potential therapeutic options. 88, 96 Vaccine development research using a variety of approaches is underway. 97 A novel human coronavirus (initially referred to as HCoV-EMC for Erasmus Medical Center in the Netherlands and now known as Middle East respiratory syndrome coronavirus [MERS-CoV]) was first identified in July 2012 in a 60-year-old man from Saudi Arabia with fatal lower respiratory tract infection. 17 As of July 2013, a total of 81 laboratory-confirmed cases of infection with this virus have been reported internationally. 98 Cases have been reported from nine countries, all with a direct or indirect link to locations in and around the Arabian peninsula. The earliest recognized cases were retrospectively identified in 2 persons in Jordan who were part of an 11-person hospital cluster of respiratory illnesses in April 2012. 99 Of the 81 confirmed cases, the median age is 55 years (range, 2 to 94 years) and 45 (56%) have died; 64% of the cases for whom gender is known occurred in males. Illness has been characterized by severe respiratory distress and pneumonia often requiring mechanical ventilation, and some cases have been accompanied by renal insufficiency. 99 A three-person cluster in Great Britain where the index case was exposed in the Middle East and disease subsequently occurred in two close contacts in Great Britain with no history of travel, as well as an instance of nosocomial transmission in France where the index case was exposed in the Middle East, provide evidence the virus can be spread person to person. 99 In addition to the 2012 Jordanian hospital cluster, another outbreak in April 2013 involving 22 persons, including health care workers, and 9 deaths in an eastern Saudi Arabian hospital suggests nosocomial transmission. 100 Genetic analyses of this novel coronavirus show it is the first lineage C betacoronavirus infecting humans and has a close relationship to betacoronaviruses HKU4 and HKU5 found in bats, including insectivorous bat species present in the Middle East. 101-103 A virus with 100% sequence homology to the MERS-CoV isolated from the 2012 Saudi Arabian index case was found in an Egyptian tomb bat (Tapho zous perforatus) trapped in the vicinity of that patient's home. 104 These findings suggest bats play an important role in the ecology of MERS-CoV and could be the reservoir for this new coronavirus. The virus targets a unique receptor and appears capable of infecting multiple mammalian cell lines, suggesting the potential for a zoonotic intermediary, and serologic evidence of infection with MERS-CoV has been reported in mammalian species. [105] [106] [107] even as influenza surveillance, including surveillance during and after the H1N1 pandemic, has improved in affected areas. Better surveillance would likely detect less severely ill individuals.

Although human illness has been identified in 15 countries, 79% of all H5N1 cases have been reported from just three locations (Indonesia, Egypt, and Vietnam) ( Fig. 14-6 ). This may reflect distribution of the virus in poultry reservoirs, local agricultural practices, intensity of surveillance, and other unknown factors. Between 2009 and 2012, human disease has only been found in six countries (Indonesia, Egypt, Vietnam, China, Cambodia, and Bangladesh). 136 Among countries with at least 20 reported cases, mortality has been highest in Cambodia (90%) and Indonesia (83%) and lowest in Egypt (36%). Reasons for variations in mortality are unclear, but data indicate that persons with H5N1 infections in Egypt are younger than those in other locations and overall mortality appears to be age dependent. In an analysis of a large dataset of H5N1 cases, mortality was reported to be six times higher in 10-to 29-year-olds than in children younger than 10 years old and almost five times higher in adults 30 years of age and older than in children younger than 10 years old. 141, 142 Early hospitalization has also been reported to reduce mortality, 141 and observational studies have suggested that early administration of oseltamivir also reduces mortality. 64, 143, 144 Differences in a variety of laboratory and clinical parameters have also been described between fatal and nonfatal cases. 64, 144, 145 It is unclear if variation in mortality rates can be attributed to specific clades or subtypes of H5N1.

Human disease from H5N1 mainly affects children and young adults, making the high mortality especially remarkable. Among reported cases, the median age has been 18 years with a range of 3 months to 81 years. 64, 141 Less than 10% of reported cases have occurred Since late 2003, the virus has spread widely and has been found in 63 countries in Africa, Asia, and Europe, with more than 7000 avian outbreaks reported to the World Organisation for Animal Health. 128 HPAI H5N1 is believed to have spread through multiple modes, including bird migration, international trade in poultry and poultry products, and illegal bird transport, although the relative contribution of these modes is unclear. 111, 129, 130 A variety of strategies, including depopulation, enhanced biosecurity and sanitary measures, and poultry vaccination, have been employed in veterinary settings to control and prevent HPAI H5N1. 120, 131, 132 However, owing to the widespread distribution of this virus, its continuous evolution into multiple lineages, poultry husbandry practices in many parts of Asia, its presence in wild birds and waterfowl, and the human and financial resources needed to implement control measures, the success of these strategies has been variable. 133 Because studies in several countries have demonstrated a strong correlation between the detection of H5N1 outbreaks in poultry and the location and timing of human disease, veterinary public health measures to control the virus must remain an integral part of efforts to reduce the risk to humans from H5N1 and other avian influenza viruses. 134, 135 From 2003 through the end of 2012, the WHO has recorded 610 human cases of H5N1 in 15 countries, with 360 (59%) of these cases being fatal-an unprecedented case-fatality rate for influenza. 136 It has been argued that the observed case-fatality rate may appear artificially high owing to restrictive case definitions and surveillance methods that miss milder and asymptomatic infections. 137 However, H5N1 serosurveys, even in high-risk poultry workers, tend to yield low seroprevalence, and this argument has been challenged. [137] [138] [139] [140] Furthermore, the case-fatality rate has not changed appreciably over time (Fig. 14-5) 

Another unusual feature of the H7N9 outbreak is the age and gender distribution of recognized cases. The median age is 61 years (range, 2 to 91 years) with 21% of cases in persons 75 years of age or older. 158, 159 In addition, males comprise 71% of recognized cases. It is unclear if these epidemiologic features result from differential exposure to the virus source or differences in population immunity, clinical presentation, or illness severity or represent surveillance artifact. However, these features stand in stark contrast to the experience with H5N1 and the 2009 pandemic, both of which predominantly impacted younger age groups.

Among H7N9 patients with available information, epidemiologic investigations suggest that most (77%), but not all, had exposure to animals (predominantly chickens and ducks) on farms or in urban wet markets. 159 Interventions that included closure and depopulation of wet markets in Shanghai coincided with a decline in newly recognized cases in that location. Clinical information on early cases indicates that virtually all (99%) required hospitalization and that most (97%) had evidence of pneumonia followed by acute respiratory distress syndrome and a majority (61%) had underlying health conditions placing them at increased risk for complications and fatal outcomes. 159, 160 Pandemic Influenza H1N1

In April 2009, two epidemiologically unlinked children living near the Mexican border in California were determined to have respiratory illness caused by a novel form of influenza A (H1N1). 161 The novel virus (referred to as pandemic H1N1 [pH1N1]) had not been previously recognized and contained six segments from influenza viruses known to be circulating in North American swine since 1998 and two segments of a Eurasian avian-like swine virus. 162 Viruses in the North American lineage that donated the segments found in pH1N1 were themselves triple reassortants, containing genetic material derived from avian, human, and classic swine viruses, and had occasionally produced human disease. 162 However, among the small number of humans identified with triple-reassortant North American swine H1 influenza, a history of close contact with pigs could usually be elicited. 163 In contrast, neither of the California children was found to have such an exposure. Both the novelty of the virus and the lack of swine contact in these children raised immediate concern that the virus was transmitted from person to person and therefore posed a pandemic threat. 16 Furthermore, only weeks earlier, Mexican health authorities had reported to the Pan American Health Organization an outbreak of severe respiratory illness associated with more than 800 hospitalizations and 100 deaths, mostly in children and young adults. 164 The same pH1N1 virus was subsequently identified in more than 40% of samples tested from Mexican patients that were part of this outbreak. 164 Epidemiologic investigations in Mexico suggested that illnesses with this virus occurred as early as February 2009 in an area of Veracruz State with nearby swine farms. 165 Within 3 weeks of its detection in the United States, more than 600 additional illnesses with pH1N1, including clusters, had been recognized in 41 states. 16 The virus was also quickly detected on other continents, 166 and by June 11, 2009 , the WHO declared that an influenza pandemic due to pH1N1 was underway. 167 Subsequent modeling studies suggest that the Mexican outbreak was much larger in scale (>30,000 cases) than initially recognized and was not nearly as severe as first reported, with an estimated casefatality rate of only 0.4%. 165 There is also evidence that pH1N1 spread quickly from its initial focus in Mexico to other locations. Among early cases in the United States, 18% had a history of recent travel to Mexico, and early cases in Europe and elsewhere had a similar travel history. 16, 166 A modeling analysis also suggested a strong relationship between air passenger traffic to and from Mexico and locations that had early importations of pH1N1. 168 By late May 2009, pH1N1 was already reported from 48 countries and the total number of identified cases was escalating quickly. Once the pandemic was declared over, a total of 214 countries, overseas territories, or communities worldwide had reported cases of pH1N1. 169 In the United States, the pandemic occurred in two waves: a spring wave was followed by a much larger autumn wave, which peaked in late October. 170 During the pandemic period, more than 99% of all subtyped influenza viruses in the United States were pH1N1. 170 The fall in persons older than 40 years of age. There has been a slight female preponderance among reported cases, and cases have most commonly been seen in the winter and early spring (mirroring patterns of poultry outbreaks). 141 The incubation period has been estimated to be up to 7 days and is most typically 2 to 5 days. 64 Virtually all human cases are sporadic, with exposure to sick and dying poultry or to poultry products being the most commonly reported risk factor (96% of cases in one series). 111, 141 In China, urban cases have been linked to exposure to poultry in live bird markets. 146, 147 A number of small clusters indicating limited person-to-person transmission have been reported. 148 In a report summarizing the experience in Indonesia, 26 case clusters were identified with an average size of 2.5 persons; the largest involved eight members of a family with three cycles of transmission. 149 The severity of illness, high mortality, and limited person-to-person transmissibility may be explained by the higher tropism of currently circulating H5N1 viruses to receptors in the lower but not upper respiratory tract. Most human illness has exhibited clinical and radiographic evidence of pneumonia, which is associated with substantial morbidity and mortality in influenza. 64, 111 Human-to-human transmissibility is a key requirement for pandemic influenza viruses, and the ability of circulating H5N1 viruses to acquire this attribute has been of keen interest to the public health community. Two recent experiments, one through in vitro genetic reassortment and the other through sitedirected mutagenesis and serial passage in ferrets, provided evidence that H5N1 can mutate in ways that facilitate transmission between ferrets, the usual surrogate for human influenza. 150, 151 These "gain-offunction" experiments produced considerable debate and international alarm, resulting in delayed publication of the studies and prompting a temporary moratorium on such research until additional safeguards were in place to minimize the potential for inadvertent laboratory release or intentional misuse. 152, 153 They also highlighted the need to continue preparedness efforts to develop countermeasures against H5N1 and other pandemic threats in case such mutations occur in nature. Neuraminidase inhibitors could be used prophylactically and therapeutically in the event of widespread H5N1 disease. In addition, a number of licensed pre-pandemic H5N1 vaccines have been produced by different approaches; in the United States, H5N1 vaccine is being stockpiled by the federal government as a contingency measure. 154, 155 In March 2013, an outbreak of influenza due to a previously unrecognized influenza A (H7N9) virus was detected in eastern China. 18 The earliest known illness associated with this outbreak occurred in Shanghai in February 2013 in an 87-year-old man. 18 This outbreak is notable for several unusual features. Analysis of the virus finds it to be a quadruple reassortant with all genetic segments originating in Eurasian lineage avian viruses (H7N3, H9N2, H9N7) with acquired mutations that enhance adaptation to humans. 18, 116, 156 The virus is of low pathogenicity to poultry and other bird species, meaning it produces minimal overt illness in bird hosts. 18 This makes it difficult to track and complicates application of control measures such as culling. Testing of avian species and environmental samples in affected areas of China found H7N9 viruses similar to those causing human illness in chickens, ducks, and pigeons, although at a very low prevalence (0.07% of 68,060 samples) given the rapid increase in recognized human cases. 157 By the summer of 2013, a total of 135 confirmed human illnesses had been detected with a 33% case-fatality rate. The number of H7N9 cases recognized after only 5 months of monitoring is higher than the greatest number of H5N1 cases seen in any year since it emerged in 1997. These H7N9 cases were found in nine contiguous eastern China provinces in addition to Beijing and Shanghai, although 79% of cases were in Shanghai and adjacent Jiangsu and Zhejiang provinces. 157 This demonstrates a wide zone of circulation in urban and rural locations either through natural spread or commercial transport and movement of poultry and other birds. An additional case was identified in Taiwan in an individual who had returned from an affected area of mainland China 3 days before illness onset. 158 As of the summer of 2013, all identified cases appeared to be sporadic except for three family clusters that could represent possible limited person-to-person spread. 159 However, monitoring of almost 1700 health care workers, family members, and close social contacts of ill individuals did not find any culture-confirmed illness to suggest that person-to-person transmission of the virus had occurred. 159 range, 7 days). 177, 183 The incubation period was similar to estimated serial intervals (mean, 2.6 to 3.9 days) for spread of illness in households, where the estimated secondary infection rate reported in studies varied from 3% to 38%; higher secondary infection rates were seen in children than in adults. 184 The basic reproductive number (R 0 , the number of additional cases generated by each infection) was estimated at 1.3 to 1.7 (which is slightly higher than seasonal disease), although reproductive numbers as high as 3.3 were found in some school settings. 183 The most common severe complication during the pandemic was viral pneumonitis or secondary bacterial pneumonia. 183 Several groups were notably at higher risk for developing severe and fatal illness. These include pregnant women, especially in the third trimester of pregnancy, which accounted for half of the hospitalizations of pregnant women. 185 Whereas approximately 1% of the population is pregnant at any time, pregnant women accounted for 6.3% of hospitalizations, 5.9% of intensive care unit admissions, and 5.7% of deaths during the pandemic and had a relative risk for hospitalization of 6.8 compared with all women of childbearing age. 185, 186 Obesity, particularly morbid obesity (body mass index >40), was also found to be a risk factor for severe disease. In the United States, morbidly obese persons with no other chronic medical conditions had a statistically significant odds of hospitalization of 4.9 and an odds of death if hospitalized of 7.6 compared with nonobese persons. 187 In a global pooled data analysis, the relative risk of death among the morbidly obese was 36.3. 186 The increased risk for severe outcomes in both pregnant women and obese persons is likely multifactorial but could include relative immune suppression or impaired pulmonary function. Another high-risk group for severe illness was children with neurologic disease. In the United States, 43% of fatalities in those younger than 18 years of age had neurologic disease; 94% of these children had neurodevelopmental disorders such as cerebral palsy and intellectual impairment. 188 A variety of nonpharmaceutical interventions, including school closure, were implemented to mitigate the impact of the 2009 influenza pandemic. Once pH1N1 was recognized to pose a pandemic threat, efforts were immediately initiated to develop monovalent vaccines. Trials in the United States showed that candidate vaccines produced using standard methods were immunogenic and safe, and large-scale production was initiated under government purchase and distribution. 189 Priority groups for vaccination representing 159 million persons were identified by the Advisory Committee for Immunization Practices and the CDC, and a more limited priority group of 62 million persons was identified for the anticipated initial limited vaccine supply (Table  14-7) . Ultimately, the first vaccine supplies did not become available until the beginning of October 2009, only weeks before the second wave of illness peaked. This was too late to have a substantial short-term impact, and allocation and distribution of limited supplies were challenging. 189, 190 Pandemic vaccine was shown to have a safety profile wave did not begin simultaneously throughout the country, and analyses suggest that its timing in a location correlated with the beginning of the school year. 171 Schools appeared to play an important role in community spread of the virus. 172 A number of countries, including the United States, promulgated school closure policies, and observational studies suggest these policies led to reductions in transmission and in the occurrence of respiratory illness. [173] [174] [175] Using a variety of data sources, the Centers for Disease Control and Prevention (CDC) estimates that between 43 million and 89 million (mid-range 61 million) illnesses due to pH1N1 occurred over the course of the pandemic (April 2009 to April 2010) in the United States, representing between 14% and 29% of the population based on 2010 U.S. census data. 176 These proportions are not substantially different from those reported in other parts of the world. 177 In the United States, these illnesses led to an estimated 274,000 hospitalizations (range, 195,000 to 403,000) and 12,470 deaths (range, 8,870 to 18,300), with a resulting hospitalization rate of 0.45% and case-fatality rate of 0.02. 176 However, these illnesses were not equally distributed by age. Children and young adults were disproportionately impacted by the pH1N1 pandemic, with 33% of all cases occurring in those younger than 18 years of age. In contrast, only 10% of all cases occurred in persons in the 65 and older age group. The estimated overall attack rate in children was 26%, and in persons older than 18 years it was 18.5%. 178 In contrast to mortality patterns with seasonal influenza, where 90% of all deaths occur in persons older than age 65 years, 87% of all influenza-related deaths in the United States during the pandemic were in persons younger than age 65 years and the median age of death was 37 years. 178, 179 However, the case-fatality rate was estimated to be more than four times higher in persons older than 65 years of age than in children younger than 18 years of age. 178 Globally, the estimated number of respiratory and cardiovascular deaths associated with pH1N1 was 284,500 (range, 151,700 to 575,500), with 80% of these deaths in persons younger than 65 years of age. 180 Serologic surveys performed in multiple locations support the findings of a higher incidence of infection in children and teenagers (20% to 60%) than in older age groups. 181 These surveys also found that the presence of cross-protective immunity in pre-or early-pandemic specimens was age dependent, being very low in children and peaking in persons older than 60 years, and suggest that older individuals were protected during the pandemic by residual immunity from exposure to pre-1950s cross-reactive H1N1 viruses. 181 Another study that examined laboratory-confirmed pH1N1 infections and hospitalizations found a marked decline in risk for persons born in the 1950s and attributed this reduction to exposure to H1N1 viruses circulating before the 1957 Asian H2N2 pandemic. 182 The clinical features of illness from pH1N1 were typical of those seen with seasonal influenza, with fever and cough most common ( Fig.  14-7) and an incubation period estimated to be 1.5 to 3 days (upper demonstrated that antiviral drugs administered early in the course of illness reduced adverse outcomes. 196 The pandemic influenza A (H1N1) virus has continued to circulate since the pandemic was declared over by the WHO in August 2010.

In 2012, a multistate outbreak of influenza caused by a swine-origin (variant) influenza A (H3N2) virus occurred in the United States, with 307 recorded cases in 11 Midwestern and Middle Atlantic states. 197 Additional single cases were identified in Utah and Hawaii. The resulting illness had typical clinical features of influenza; 16 cases (5%) required hospitalization and one fatality occurred in an adult. 197 However, most cases (93%) were recognized in persons younger than 18 years, and the median age was 6 years. The outbreak occurred between July and September during the major period when agricultural and state fairs that exhibit swine occur. 198 Most ill persons (>90%) had a history of participation in or attendance at these events, with a history of direct or indirect contact (often sustained) with swine, and there was evidence of only limited person-to-person transmission. 198 Similar H3N2v viruses were identified in exhibited swine in some locations. 198 In response, the CDC recommended that persons at high risk for complications of influenza, including individuals younger than 5 and those 65 years of age and older, avoid contact with swine or swine barns at agricultural fairs during 2012.

The variant H3N2 virus is a descendent of a lineage of a triplereassortant H3N2 virus with human, avian, and swine segments that began widely circulating in North American pigs in 1997-1998. 199 The first human infection caused by this variant was not recognized until 2005 in Kansas, and only sporadic human cases were recognized before 2011. 200 In 2010, the virus was observed to have acquired the M (matrix) segment from the pandemic H1N1 virus (Fig. 14-8) and to have become more frequent in swine, raising concerns about enhanced transmissibility and an increased burden of disease in humans. 201 After that reassortment event was recognized in swine, 12 human cases were recognized in 2011 in five states. 202 Similar to the larger 2012 outbreak, disease occurred mostly in children and agricultural event exposure was prominent with limited person-to-person transmission. 203 Serologic studies demonstrated little cross-protective immunity in young children. 204 In contrast, a significant proportion of older children and adults appeared to have such immunity, likely owing to similarities with H3N2 viruses that circulated in the 1990s and earlier. 204 Studies show that current trivalent influenza vaccines offer little protection against the variant H3N2 virus. 204 The emergence of this virus provides a further demonstration of the critical role swine play in influenza similar to seasonal trivalent influenza vaccine, and studies generally showed 70% to 90% effectiveness against laboratory-confirmed disease. 189, 191 Through the end of 2009, approximately 81 million doses of vaccine were shipped and 61 million doses were administered, with 74% given to priority targeted groups. 192 Neuraminidase inhibitors were widely employed for treatment, with an estimated 8.2 million prescriptions filled during the pandemic. 193 Investigational intravenous peramivir and zanamivir were also successfully used in almost 1500 persons in the United States. 194, 195 A number of observational studies Cryptosporidium parvum is principally waterborne. Determination of the patterns of distribution of enteric pathogens in a geographic area may suggest the relative importance of different modes of transmission in that locale.

The causes of diarrheal disease do not remain static over time, even in a single location. Fluctuations may result from the introduction of an organism not previously present, the recognition of a new agent, changing levels of sanitation resulting from man-made or natural disasters, climatic variations, or lifestyle changes, such as increases in recreational water exposure. 221, 222 Two dramatic examples from the past 25 years involve the introduction of Vibrio cholerae O1 into the Western Hemisphere. Cholera had not been recognized in South America during the 20th century, but in January 1991 cases were identified in coastal areas of Peru. 223 Within weeks, thousands of cases were occurring and cholera quickly became the most commonly diagnosed cause of diarrheal illness in many parts of Peru. 224, 225 During the next 3 years, the disease spread throughout mainland South and Central America, significantly altering the distribution patterns of etiologic agents of diarrheal disease. The incidence of the disease in Latin America has subsequently declined dramatically.

In October 2010, just 9 months after a severe earthquake, a cholera epidemic was identified in Haiti that spread within 2 months to the entire country and across the border to the Dominican Republic. 226, 227 Molecular epidemiologic studies have indicated that the V. cholerae strain is very similar to South Asian strains, 228 and epidemiologic investigations revealed that initial cases occurred downstream from a camp where United Nations peacekeepers from Nepal were stationed. 229, 230 The source of introduction of the epidemic strain remains a politically sensitive topic. 231 Although the case-fatality rate has been reduced, the epidemic persists. 232 Cases in Haiti and the Dominican Republic accounted for more than 60% of the global reported cases to the WHO in 2011. 233 In addition to cases in the Dominican Republic, imported cases have been identified in the United States 234 and elsewhere in the Western Hemisphere. 233 V. cholerae O139 is an example of a recently recognized pathogen that had a major impact on the distribution of diarrheal diseaseproducing pathogens in Asia. This serotype was first detected in South Asia in 1992 and quickly spread to many regions of India and Bangladesh. 235, 236 Studies conducted in Bangladesh suggested that shortly after its introduction V. cholerae O139 became the agent most commonly linked to clinical cholera in that country. Since then, its impact has fluctuated in place and time throughout South and Southeast Asia [236] [237] [238] ; in 2011, only China reported cases to the WHO. 233 One other emerging issue with V. cholerae merits mention. Variant El Tor strains that express the cholera toxin produced by classic strains resulting in more severe illness have been identified, initially in Bangladesh and more recently elsewhere in Asia, Africa, and Hispaniola. 233, 239 The international movement of foods can also alter the spectrum of diarrheal pathogens. In 1996, thousands of cases of cyclosporiasis due to Cyclospora cayetanensis occurred in the United States and Canada among persons who consumed fresh raspberries imported from Guatemala. 240 Before this episode, only small numbers of cases, primarily associated with travel to developing countries, 241 had been reported in North America. In 2008, a large nationwide foodborne outbreak caused by Salmonella enterica serotype Saintpaul occurred in the United States. Initial investigation suggested that contaminated tomatoes imported from Mexico were the source. However, subsequent investigation implicated jalapeño and serrano peppers from Mexico. 242 International spread of a multidrug-resistant clone of S. enterica serotype Kentucky with high-level resistance to ciprofloxacin has been reported. 243 The clone appears to have originated in Egypt during the 1990s and has spread to other countries in Africa and the Middle East. Cases that are predominantly travel associated have also been identified through national surveillance systems in France, England and Wales, Denmark, and the United States. 243 Escherichia coli O157:H7 (Shiga toxin [ST]-producing strains [STEC]) significantly affected pathogen distribution patterns in developed parts of the world after the agent was first recognized. 244 After its recognition in the United States in 1982, this foodborne agent rapidly became the most commonly identified cause of bloody diarrhea in virus evolution and the need for a "One Health" approach to influenza prevention and control (see "Vector-borne and Zoonotic Diseases").

Human bocaviruses were first detected in 2005 in Sweden when investigators used molecular virus screening methods by taking cell-free, filtered supernatants of stored respiratory specimens looking for virus-sized particles and then performing random polymerase chain reaction (PCR) amplification of the genetic material. 62 Among the sequences identified was a previously unrecognized parvovirus most closely related to animal viruses of the genus Bocavirus. Since the original identification of bocavirus DNA in respiratory specimens, three additional bocaviruses have been found in stool specimens; these agents are now referred to as HBoV1 to HBoV4. HBoV2 to HBoV4 are believed to be associated with gastrointestinal illness, whereas HBoV1 causes predominantly respiratory disease. 205 Human bocaviruses have been challenging to study because of the lack of in vitro or animal models. However, numerous studies suggest HBoV1 can be found in 2% to 19% of patients with acute respiratory tract infections, and, as often as 83% of the time, is present with other coinfecting respiratory pathogens. 205, 206 This latter finding calls into question whether HBoV1 is a pathogen, especially because HBoV1 has been shown to persist in respiratory specimens for months after acute infection, meaning it can be detected in asymptomatic individuals. 205, 206 However, in most studies HBoV1 has been found more commonly in children with acute respiratory disease than asymptomatic children; copy numbers of the virus are higher in monoinfections than coinfections and are usually low in asymptomatic children. Serology also suggests HBoV1 is a pathogen. [205] [206] [207] Children aged 6 to 24 months are most commonly infected; serologic studies show that almost all children have evidence of previous HBoV1 infection by 6 years of age. 205, 207 Illness is most common in winter. Infection can produce either upper or lower respiratory tract infection and in children is often accompanied by wheezing; pneumonia and bronchiolitis can also be seen. 205, 206, 208 Of note, DNA has been detected in serum and urine of children with acute infections, suggesting systemic infections can occur. 205 

Diarrheal disease is the second leading cause of infectious disease morbidity and third leading cause of mortality worldwide, resulting in an estimated 90 million disability-adjusted life years and 1.4 million deaths in 2010. 5, 6, 209 Although diarrheal disease affects persons of all age groups and in all geographic locations, the greatest burden of severe illness and death falls on infants and young children in developing countries. In 2010, diarrhea ranked third behind malaria and lower respiratory tract infections among the leading causes of death in children younger than 1 to 4 years of age. Undernutrition is an important underlying cause of many of these deaths. 210 Diarrheal disease also produces substantial morbidity, affecting growth and cognitive development 211, 212 and resulting in illness of more than 2 weeks in duration in 10% of cases. 213 Studies among children in developing countries have found a range of 1 to 10 episodes of diarrhea per child each year, with most children experiencing 3 to 5 episodes per year. 214, 215 A survey among the general U.S. population reported a rate of 1.4 episodes of diarrhea per year, translating to 200 to 375 million episodes annually. 216 The pathogens that produce diarrhea are transmitted primarily by three main routes: foodborne, waterborne, and person to person; many enteric pathogens can be transmitted by more than one route, including simultaneously. Although systematic studies are difficult to perform, it is likely that the relative importance of these transmission patterns varies in different settings. Estimates in the United States suggest that 48 million episodes of foodborne illness occur annually, resulting in approximately 128,000 hospitalizations and approximately 3,000 fatalities 217, 218 and that endemic waterborne disease results in 4.3 to 32.8 million cases of acute gastrointestinal illness annually. 219, 220 The pathogens that cause diarrheal illness differ in their primary modes of transmission. For example, nontyphoidal Salmonella species and Campylobacter jejuni are transmitted principally through food, Shigella species are transmitted primarily from person to person, and than age 2 years, who often develop dehydrating diarrhea from the infection. 274, 275 As recently developed rotavirus vaccines are introduced into more countries in Africa and Asia, the impact of rotavirus infection on the total diarrheal disease burden will decrease.

Rotavirus is also an important cause of childhood diarrhea and hospitalization in developed countries. 274 In the United States in recent years, rotavirus infection has resulted in an estimated 55,000 to 70,000 hospitalizations, 205,000 to 272,000 emergency department visits, and 410,000 physician visits and has caused an estimated 20 to 60 deaths per year. 276 After licensure of a new rotavirus vaccine and recommendations for its use in infants in early 2006, surveillance indicated that the 2007-2008 rotavirus season had a delayed onset and a reduction in disease burden. 277 A recent study during the 2009-2010 and 2010-2011 rotavirus seasons documented the effectiveness of both rotavirus vaccines in reducing the risk for emergency department visits and hospitalizations combined in children younger than 5 years of age. 278 As a result, noroviruses have now replaced rotaviruses as the leading cause of medically attended gastroenteritis in U.S. children. 266 Systematic studies in the United States indicate that among the bacterial diarrheal pathogens, C. jejuni was the most commonly diagnosed agent in the early 1990s, followed by nontyphoidal Salmonella species, Shigella, and E. coli O157:H7. 245 More recent data from FoodNet sites for 2012 indicate that Salmonella serotypes are now the most common bacterial pathogen, followed by Campylobacter and Shi gella. 247 In 2012, the incidence of E. coli serotypes other than O157:H7 exceeded that of E. coli O157:H7 strains. The incidence of Campylo bacter and Vibrio infections was higher in 2012 compared with that in 2006 to 2008, while the incidence of infections caused by most major foodborne bacterial pathogens was unchanged. 247 Continued surveillance along with detailed information on patient exposures and strain subtypes can help assess the impact of control measures on Campylo bacter infections, including new standards for chicken and turkey processors issued by the U.S. Department of Agriculture in 2011. In addition, despite a significant increase in Vibrio infections, the number of these infections continues to be low. 247, 279 Foodborne sources remain an important cause of bacterial diarrheal disease in the developed world, because all but Shigella species are transmitted principally through foods. Notifiable disease data demonstrate trends in the occurrence of these pathogens but severely underestimate their incidence. As an example, 45,970 cases of salmonellosis were reported in the United States in 1995 but estimates suggest that almost 2 million U.S. infections with the bacteria occur each year. 280 The incidence and severity of Clostridium difficile-associated disease has recently increased dramatically in adults and children in the United States, Canada, Europe, and Australia. [281] [282] [283] [284] 285 In the United States during 2010 in Emerging Infections Program sites, 94% of C. difficile infections were health care associated whereas 6% occurred in patients with no recent health care setting exposure. 283 Disease due to a strain of restriction endonuclease analysis group BI, pulsed-field gel electrophoresis type NAP1, and PCR ribotype O27 (BI/NAP1/O27) is responsible for much of this increase, including many nosocomial outbreaks. [286] [287] [288] Two distinct epidemic lineages, both of which are resistant to fluoroquinolone antibiotics, have recently been identified. 284 The FQR1 lineage, first seen in 2001, is associated with North American outbreaks and sporadic infections in South Korea and Switzerland. The FQR2 lineage originated in 2003 in North America but subsequently spread more widely, causing hospital outbreaks in the United Kingdom, continental Europe, and Australia. 289 Of the HAIs, 75% of persons had onset of illness outside acute care hospitals (in the community or in nursing homes). 289, 290 These strains can cause severe disease, particularly in elderly patients. Since 2005, another C. difficile strain (PCR ribotype O78) has emerged in the Netherlands and in the United Kingdom. 291 This strain also appears to be hypervirulent and is more likely to affect younger persons and to be associated with community-onset disease. 291 Isolates of C. difficile also have been obtained from retail ground meat samples, 292 prompting the need for evaluation of the potential role of foodborne transmission in community-associated cases. Recent experiences with fecal microbiota transplantation have suggested an important role for this approach in the management of patients with severe C. difficile infections. 293, 294 many locations in North America. 245 Surveys conducted in the 1990s and in 2007 found it was the fourth most commonly isolated bacterial agent of diarrheal disease in the United States. 245, 246 In 2012, STEC non-O157 strains ranked fifth and STEC O157 strains ranked sixth in incidence per 100,000 population among laboratory-confirmed cases of foodborne infections in the 10 states participating in the Foodborne Diseases Active Surveillance Network (FoodNet). 247 In early May 2011, a large outbreak of bloody diarrhea and hemolytic-uremic syndrome was recognized in northern Germany. 248, 249 Investigation identified a unique single clone of E. coli O104:H4 as the etiologic agent. 249, 250 The strain contained a prophage encoding Shiga toxin 2, genes from enteroaggregative E. coli, and a plasmid-encoded extended spectrum β-lactamase gene. 249, 251 Epidemiologic investigation identified fenugreek sprouts as the food vehicle 252, 253 ; the sprouts were grown from seeds of Egyptian origin. 249 The outbreak involved more than 4000 illnesses, primarily in adults, 254 approximately 800 cases of hemolytic-uremic syndrome (HUS), and 50 deaths in Germany and 15 other countries 249 and provides yet another reminder of the global nature of today's food supply.

Outbreaks of diarrheal diseases are particularly affected by deteriorations in public health infrastructure, as dramatically illustrated by the introduction of cholera into Haiti after the 2010 earthquake. Shigellosis is known to emerge rapidly in areas with social disruption caused by war and political unrest, especially when large numbers of refugees have lacked access to water and sanitation services. 255 As a result of the Rwanda crisis in 1994, cholera outbreaks in the refugee camps in Goma, the Congo, caused at least 48,000 cases and 23,800 deaths within 1 month. 256 Similarly, a large cholera outbreak occurred in Zimbabwe in 2008-2009 during a period of political turmoil and economic collapse. 257, 258 The disease spread across borders to South Africa, Botswana, and Mozambique. 258 Viruses are an increasingly recognized cause of sporadic and outbreak-associated diarrheal diseases. Noroviruses (members of the Caliciviridae) are transmitted not only from person to person but also by food and water and by contact with contaminated environmental surfaces. [259] [260] [261] These viruses have become an important cause of diarrheal illness outbreaks among cruise ship passengers, hospitalized patients, nursing home residents, college students, restaurant patrons, and military personnel, reflecting the high infectivity and low infectious dose (<20 viral particles). 262 A systematic literature review published in 2008 indicated that severe norovirus disease occurs among persons in both developed and developing countries, annually resulting in an estimated 64,000 episodes of diarrhea requiring hospitalization and 900,000 clinic visits by children in developed countries and as many as 200,000 deaths among children younger than 5 years of age in developing countries. 263 Noroviruses are the leading cause of epidemic gastroenteritis in the United States. 264, 265 After the introduction and use of rotavirus vaccines, noroviruses have also become the leading cause of medically attended acute gastroenteritis in U.S. children younger than 5 years of age; these viruses were found in 21% of children seeking medical care for acute gastroenteritis in 2009-2010. 266 New strains emerge every few years. In March 2012, a new GII.4 norovirus strain named GII.4 Sydney was identified in Australia and subsequently spread to the United States, United Kingdom, and a number of other countries. 267 GII.4 norovirus outbreaks have been associated with higher rates of hospitalization and mortality compared with outbreaks caused by other genotypes. 268 Because the pathogens responsible for diarrheal disease vary over time, even in the same location, longitudinal studies are important for defining the etiology of diarrheal disease. Data from the Global Enteric Multicenter Study (GEMS) conducted using standardized methods 269-271 over 3 years in seven developing countries in Africa and Asia indicated that the leading pathogens in children younger than 5 years of age with moderate to severe diarrhea across all sites were rotavirus, Cryptosporidium, enterotoxigenic E. coli strains producing heat-stable enterotoxin, and Shigella. 272 Compared with control children, those with moderate-to-severe diarrhea had an 8.5-fold increased risk for death during follow-up. 272 Rotavirus infections affect all age groups and have been the leading cause of severe acute diarrhea among young children worldwide. 273 Most severe illnesses and hospitalizations occur in children younger enterotoxigenic Bacteroides fragilis, 319 V. cholerae serogroups O75 320 and O141, 321 bocavirus species HBoV2, 322 Klebsiella oxytoca in patients with antibiotic-associated diarrhea 323 and hemorrhagic colitis, 324 and enterohemorrhagic E. coli O26:H11/H− strain recently identified in Europe 325 require further evaluation. Use of advanced molecular diagnostics and other emerging technologies may be useful in assessing the role of currently unrecognized etiologic agents and alterations in the intestinal microbiome in patients with sporadic unexplained diarrheal illness and Brainerd diarrhea. 326, 327 Patients who develop traveler's diarrhea while abroad 306, 307 and those who develop unexplained diarrhea in hospital settings 328 represent ideal sentinel populations for further study to identify additional etiologic agents. Certain high-risk foods such as raw milk 329 and unpasteurized cheeses 330,331 require continual attention from public health authorities. Because recent antimicrobial exposure has been identified as a risk factor for acquisition of enteric infections in animal models 332 and in humans, 333, 334 continued emphasis on judicious antimicrobial use is critical. Finally, because human contact with animals and their environments remains an important mode of acquisition of important enteric pathogens, especially Salmo nella, Campylobacter, and Cryptosporidium, [335] [336] [337] [338] [339] increased interdisciplinary collaborative efforts at the human, animal, environmental interface as promoted by the "One Health" model are a priority (see "Vector-borne and Zoonotic Diseases").

Most of the emerging infections recognized during the past decade have been zoonoses (see Table 14 -2). 3,340 Some zoonotic microbes have "jumped" from animals to humans to become major human pathogens (e.g., the simian virus that evolved into HIV 341 ), whereas others are maintained in animal reservoirs, including domesticated animals (e.g., cattle infected with Rift Valley fever virus 342 ) or wildlife (e.g., bat species that carry Nipah or Hendra viruses 343 and monkeys and rodents that carry monkeypox 344 ). Bats have become an increasingly important source of emerging infections and have been linked to SARS coronavirus as well as Nipah, Hendra, and Ebola and Marburg viruses. Some vector-borne diseases are also zoonotic (e.g., West Nile disease, caused by a mosquito-borne virus maintained in birds, or Lyme disease, caused by a tick-borne bacteria maintained in deer and rodents), whereas for others humans are the principal host (e.g., dengue and human species of malaria).

Emerging public health concerns related to vector-borne and zoonotic infections include the geographic spread of mosquito-borne diseases such as dengue, chikungunya, and West Nile fever, the rising incidence of tick-borne infections such as Lyme disease, and recurring outbreaks of Ebola and Marburg hemorrhagic fever in Uganda and the Democratic Republic of the Congo. 345 In addition, animal influenza strains that cause disease in humans (e.g., avian influenza A [H5N1], 346 203, 348 ) remain ongoing concerns because of their potential to evolve into pandemic strains. The origin and reservoir of a recently identified coronavirus, named Middle Eastern respiratory syndrome coronavirus (MERS-CoV), although presumed to be a zoonotic infection, has not yet been identified (see "Acute Respiratory Tract Infection").

The recognition that the majority of new human pathogens emerge from animal reservoirs has led to increased medical, veterinary, and scientific support for a "One Health" approach to infectious disease control, a growing consensus about the importance of intensifying efforts to link infectious disease identification, prevention and control at the human, animal, and environmental interface. 349, 350 Areas of "One Health" focus include identifying emerging drug resistance in zoonotic pathogens that infect domestic animals and humans; evaluating the efficacy of veterinary vaccines in reducing the incidence of animal pathogens that can infect humans (e.g., Rift Valley fever virus 351 ); and developing new methods and strategies to prevent infections carried by ticks or mosquitoes.

Public health and animal health scientists are also exploring strategies for predicting and preventing the emergence of novel zoonotic pathogens, making use of pathogen discovery tools and mathematical modeling techniques. 352 These efforts require detailed understanding Multiple drug resistance in bacterial enteric pathogens has emerged in the United States and internationally. The problem is most severe in foodborne pathogens of animal origin (primarily in Campylobacter and Salmonella strains). 295 A multidrug-resistant strain of S. enterica serotype Typhimurium known as definitive type 104 (DT104) emerged in the United States and Europe in the 1990s. 296, 297 Patients infected with multiply-resistant strains are more likely to be hospitalized. 298 Fluoroquinolone-resistant strains of C. jejuni infections have also emerged; some infections are associated with foreign travel whereas others are acquired domestically and have been associated with poultry consumption. 299 In a study of resistance in over 1100 Shigella isolates in FoodNet sites from 2008 to 2010, 74% were resistant to ampicillin, 36% to trimethoprim-sulfamethoxazole, 28% to tetracycline, and 0.5% to ciprofloxacin 300 ; 5% of isolates were resistant to five or more antimicrobial agents. 300 Resistance was more common in persons with a history of recent foreign travel. A foodborne outbreak in Los Angeles in 2012 was caused by Shigella sonnei with decreased susceptibility to azithromycin, the first such outbreak identified in the United States. 301 The global pattern of diarrheal disease is likely to continue to evolve, and efforts to develop effective vaccines against causative agents are ongoing. New formulations of rotavirus vaccine have been developed after an initial tetravalent rhesus/human reassortant rotavirus vaccine was linked to intussusception in infants and was withdrawn from the market. 302 These new rotavirus vaccines have the potential to decrease the burden of diarrheal disease among persons in the developing world, and a major effort is in progress to introduce these vaccines into all national immunization programs as recommended by the WHO. 303 In addition, food production practices are changing, with greater amounts of fresh fruits and vegetables grown in the developing world for export to developed countries. Such practices have resulted in the transfer of agents such as C. cayetanensis, V. cholerae, and S. enterica serotype Enteritidis in exported products-a situation that is likely to continue. 240, 304, 305 Changes in food distribution practices increase the potential for widespread, multinational disease outbreaks. The increasing numbers of immunocompromised individuals who are at risk for infections with a broader array of pathogens responsible for diarrheal illness and increasing global travel will likely influence future trends in diarrheal disease in ways that may be difficult to predict. 306, 307 Another example is the emergence of a new strain of multidrugresistant S. enterica serotype Typhimurium (multilocus sequence type ST313) in sub-Saharan Africa. The strain causes invasive nontyphoidal disease, sometimes accompanied by diarrhea, primarily in patients with HIV infection, malaria, and malnutrition. 308, 309 Application of whole-genome sequence-based phylogenetic methods has identified two closely related, highly clustered lineages estimated to have emerged independently approximately 52 and 35 years ago. Lineage II strains have replaced lineage I strains, perhaps because of their acquisition of chloramphenicol resistance. 309 Climate change has been reported to affect the spread of cholera and Vibrio parahaemolyticus infections. In 2004, a large outbreak of V. parahaemolyticus infection associated with consumption of raw oysters occurred among passengers aboard a cruise ship in Prince William Sound in Alaska. 310 The oysters were harvested in the Sound during summer months when daily water temperatures exceeded 15° C. This outbreak extended by 1000 km the northernmost documented source of oysters associated with V. parahaemolyticus infection.

Efforts to decrease the emergence and enhance the control of diarrheal disease and associated antimicrobial resistance in the United States and internationally require strengthened disease surveillance systems to monitor disease trends and changes in food consumption patterns. Advances in molecular diagnostic techniques for rapid diagnosis and characterization of isolates 311,312 offer advantages over current techniques, but the shift to this new paradigm away from pathogen isolation will pose challenges in the near term for many current infectious disease surveillance systems that rely on characterization of bacterial isolates. 313, 314 Interstate foodborne disease outbreaks will continue to pose challenges, [315] [316] [317] as will the international spread of drugresistant enteric pathogens. 243 The role of food in the transmission of drug-resistant organisms will require continued attention. 318 The potential importance of several enteric pathogen candidates such as only sporadically in some Caribbean islands during this period. The success of the program, however, led to its gradual discontinuation beginning in 1970. Since that time, A. aegypti has reestablished itself firmly in the region, now exceeding the extent of distribution that was seen before the eradication program began (Fig. 14-9 ). Epidemics of dengue fever now routinely occur in Venezuela, Colombia, Brazil, and other locations in Latin America and the Caribbean. 359 Puerto Rico, which has experienced epidemic dengue activity periodically since 1963, suffered the largest dengue outbreak in its history in 2010, affecting more than 21,000 people. 360 In 2009 and 2010, Florida reported the first cases of dengue acquired in the continental United States outside the Texas-Mexico border since 1945. 361 Multiple outbreaks in Africa were identified in 2013. 362 A number of lines of evidence suggest that dengue is endemic in Africa on a par with the Americas but is not well recognized. 355, 363 No therapy is effective for dengue infection, and treatment is supportive, with particular attention to fluid management. 354 In Puerto Rico, deaths from dengue were reduced by training physicians in early detection and appropriate supportive care of patients with dengue hemorrhagic fever. 364 A U.S. Food and Drug Administration (FDA)approved, real-time polymerase chain reaction (RT-PCR) assay that can detect all four dengue serotypes in the first 7 days after symptom onset was developed by the CDC and is available from the agency at the time of this writing, although wider availability through commercial sources is desirable (www.cdc.gov/dengue/resources/rt_pcr/ CDCPackageInsert.pdf28).

It has been a long-standing challenge to develop a safe dengue vaccine that is effective against all four serotypes, complicated by concerns that vaccination might predispose to the severe form of dengue infection and by the poor understanding of the immunologic correlates of immunity. However, there has been considerable progress in the recent past and a number of candidate dengue vaccines are currently being evaluated, including live attenuated, inactivated, chimeric, DNA, and viral-vector vaccines. 365 Results of a phase IIb trial of the vaccine candidate farthest along in clinical trials, a recombinant live-attenuated tetravalent vaccine manufactured by Sanofi Pasteur, showed that the vaccine was safe but the protective efficacy was only 30.2% with a confidence interval that included zero. 366 Several other candidate vaccines currently are in phase II trials.

Efforts to reverse the recent trend of increased epidemic activity and geographic expansion of dengue are not promising. New dengue virus strains and serotypes will likely continue to be introduced into many areas with high population levels of A. aegypti. In the absence of a vaccine or new mosquito control technology, public health authorities have emphasized disease prevention and mosquito control through community efforts to reduce larval breeding sources. Improved of specific ecologic and behavioral factors that facilitate the introduction of animal pathogens into human populations (e.g., increased human presence or other changes in wildlife habitats) and allow newly introduced pathogens to become established in new areas (e.g., suitable insect vectors). 353 Dengue Dengue is the most important mosquito-borne viral disease that affects humans. Its global distribution is comparable to that of malaria, and an estimated 2.5 billion people live in areas at risk for epidemic transmission. The disease is endemic in Africa, the Americas, and parts of the Middle East, Asia, and the western Pacific. The frequency of dengue and its more severe complications-dengue hemorrhagic fever (DHF) and dengue shock syndrome-has been increasing dramatically since 1980. 354 It is generally estimated that 50 to 100 million infections occur annually, but recent data suggest that approximately 390 million dengue infections occurred worldwide in 2010, including 96 million symptomatic illnesses. 355 Dengue is caused by any of four antigenically distinct virus serotypes (DEN-1, -2, -3, -4) of the genus Flavivirus. Infection with one of these serotypes is not cross protective. Infection with dengue viruses produces a spectrum of clinical illness that ranges from a nonspecific viral syndrome to severe and fatal hemorrhagic disease. 356 DHF is a life-threatening condition characterized by capillary permeability that may lead to hypovolemic shock and death. Important risk factors for DHF include the strain and serotype of the infecting virus, as well as the age, immune status, and genetic predisposition of the patient. In endemic areas, most deaths from dengue infection occur in children younger than 15 years of age. 356 Economic impact analyses in both the Americas and Asia indicate that the burden of dengue-related illness in children is substantial, especially when nonhospitalized patients are considered. 357, 358 Dengue is transmitted primarily by Aedes aegypti, a domestic, daybiting mosquito, and secondarily by A. albopictus. A. aegypti was historically found in Africa but spread throughout the tropical regions of the world during the past 2 centuries through international commerce. It is well adapted to the urban environment, feeding on humans and breeding in containers in areas where water is stored or allowed to accumulate, such as discarded cans, bottles, plastic containers, and tires. Epidemics caused by multiple serotypes (hyperendemicity) have become more frequent, and the geographic distribution of both the viruses and their mosquito vectors has expanded.

The emergence of dengue and DHF in the Americas has been dramatic. In an effort to prevent urban yellow fever, which is also transmitted by A. aegypti, the Pan American Health Organization established a program in the 1950s and 1960s to eradicate the species from most of Central and South America. 353 As a result, epidemic dengue occurred the continental United States, becoming endemic and the leading cause of arboviral disease in the country-affecting thousands of people each year. 388 394 Texas was at the epicenter of the 2012 outbreak, 395 reporting about one third of all cases. It is estimated that a cumulative 2 million to 4 million infections and 0.4 million to 1 million resulting illnesses occurred in the United States from 1999 to 2010. 389 WNV is a single-stranded RNA virus of the Flaviviridae (genus Flavivirus), which is part of the Japanese encephalitis virus antigenic complex. In addition to Japanese encephalitis, this complex includes St. Louis encephalitis virus, Murray Valley encephalitis virus, and Kunjin virus, a subtype of WNV. 382 WNV is transmitted to humans primarily through the bite of infected mosquitoes, but person-to-person transmission can occur through transfusion of infected blood products or solid-organ transplantation. [396] [397] [398] Because the U.S. blood supply has been routinely screened for WNV RNA since 2003, transfusion-associated WNV infection is rare.

The virus is maintained in a bird-mosquito-bird cycle, with birds developing high levels of viremia and serving as amplifying hosts. Mosquitoes primarily of the genus Culex transmit WNV, although the virus has been isolated from many genera and species of mosquitoes. Although most species of infected birds generally remain asymptomatic, WNV-related mortality has been noted in more than 160 avian species in the United States and Canada. 399 Crows and related birds of the Corvidae are especially susceptible to mortality from WNV infection, and die-offs of these birds have been used as one indicator of active WNV transmission. 399 Equines are frequently infected with WNV, with 36 U.S. states reporting equine infections in 2009. 400 Approximately 80% of WNV infections in humans are asymptomatic, 20% develop fever, and less than 1% are WNV neuroinvasive disease. Most symptomatic persons have an acute febrile illness consisting of headache, myalgia, or arthralgia that lasts from 3 to 6 days. 401 WNV neuroinvasive disease is characterized by acute neurologic manifestations, usually encephalitis, meningoencephalitis, or acute flaccid paralysis. 384, 396, 402 The mortality rate from WNV neuroinvasive disease is approximately 10%. Long-term outcome among survivors varies, with some persons showing little neurologic and functional improvement and others experiencing substantial gains. 403 Persons suffering paralytic disease with respiratory involvement are at greatest risk for death and have a poor long-term outcome. [401] [402] [403] Although WNV strains circulating in the United States have genotypic differences and the predominant circulating strain has changed over time, 404, 405 no strain-specific differences in virulence or clinical disease in humans have been documented, and no antigenic differences have been identified that would pose a challenge to vaccine development. Nevertheless, the sporadic and unpredictable pattern of WNV incidence represents a considerable barrier to randomized clinical trials of vaccines or treatments. 406 Although vaccines for use in horses are licensed-and phase I and II clinical trials of candidate vaccines for human use have been completed-no phase III trials in humans have been attempted. 407 At the present time, prevention of WNV infection relies on a cadre of efforts that include mosquito, bird, and human disease surveillance; mosquito control; the use of personal protective measures; and screening of the blood supply. There is no treatment of proven efficacy for WNV infection. 392

Ebola and Marburg hemorrhagic fevers are severe, often-fatal diseases in humans and nonhuman primates (monkeys, gorillas, and chimpanzees). Symptoms include fever, headache, joint and muscle aches, sore throat, and weakness, followed by diarrhea, vomiting, and stomach pain. Some patients also exhibit a rash, red eyes, hiccups, and internal and external bleeding. There is no vaccine or standard treatment for Ebola or Marburg. Supportive therapy involves balancing fluids and electrolytes, maintaining oxygen status and blood pressure, and providing treatment for any complicating infections. 408 laboratory-based surveillance systems and other innovative approaches that can provide early warning of a potential dengue epidemic are also needed to alert the public to take protective action and physicians to diagnose and properly treat cases.

Chikungunya shares many similarities with dengue, both in terms of epidemiology and clinical illness. Like dengue virus, chikungunya virus is transmitted by A. aegypti and Aedes albopictus mosquitoes and is characterized by acute onset of fever, joint and muscle pain, headache, nausea, fatigue, and rash. Indeed, chikungunya is frequently misdiagnosed as dengue, and outbreaks of the two diseases can occur simultaneously. 367 Chikungunya is, however, caused by a member of the family Togaviridae, genus Alphavirus. Chikungunya virus infection is noteworthy in that joint pain is a prominent feature of the disease, a condition that may persist for months. Joints most commonly involved include ankle, knee, wrist, and small joints of the hands. Chikungunya was first detected in Tanzania in 1953 and spread to other parts of Africa and parts of Asia. 368, 369 Chikungunya virus is an important cause of febrile illness 370 in Africa and Asia, including the Indian subcontinent. Epidemic cycles occur with interepidemic periods ranging from 4 to 30 years. 371 Since 2004, chikungunya has caused large outbreaks in a wide range throughout Asia and Africa. In 2004, an outbreak was identified in Kenya, and subsequently outbreaks occurred in Indian Ocean islands and South India during 2005-2006. 367, 369 These outbreaks were characterized by high clinically apparent attack rates and were noteworthy in that higher than expected crude death rates occurred among affected populations, especially among the oldest age groups. 372 From March 2005 through April 2006, an estimated 255,000 cases of chikungunya occurred in Réunion (population 770,000), and excess deaths were recorded, suggesting a case-fatality rate of approximately 1 in 1000, mainly in persons 75 years of age and older. 372 Similar elevated mortality rates were seen among the elderly in Mauritius during the 2006 outbreak. 373 In India, more than 1.25 million suspect chikungunya cases were reported from 151 districts in eight states in 2006, with attack rates reaching 45% in some communities. 367 Between July and September 2007, an outbreak of chikungunya occurred in northeastern Italy, the first chikungunya outbreak to occur in a temperate region. The index case was likely a traveler from India who developed symptoms while visiting relatives in the outbreak region. More than 200 suspected cases were reported between July and September 2007, and chikungunya virus sequences were detected in pools of A. albopictus mosquitoes by PCR assay. 374 Humans infected with chikungunya virus have viremias of sufficient titers to infect feeding vector mosquitoes and are thus an ideal source for virus dissemination and introduction into new regions. In addition, one of the principal vectors, A. albopictus, has been introduced into many areas of Europe and North America, raising concern that temperate and tropical regions around the world may be receptive to future outbreaks of chikungunya. 375 Recent studies suggest that the virus is adapting to facilitate transmission by this species. 376 Preparedness and response strategies focus on early detection of cases, timely and appropriate epidemiologic investigation, and the institution of measures to mitigate spread. 371 The recognition of chikungunya as an emerging health threat has also stimulated innovative research on vaccine development [377] [378] [379] and on new antiviral therapies that involve viral or host targets. 380 

West Nile virus (WNV) is another example of a vector-borne pathogen that has spread rapidly into new areas. WNV was first isolated in 1937 from an apparently healthy individual from the West Nile district of Uganda. 381 For decades, WNV was recognized as an important endemic and occasionally epidemic disease in Africa and the Middle East, causing primarily minor febrile illness. 382 However, since 1998, major outbreaks associated with severe neurologic disease have occurred in Romania, 383 Russia, 384 Israel, 385 the United States, 386 and Canada. 387 The disease was first reported in North America in 1999 when the virus caused an outbreak of severe neuroinvasive disease that resulted in seven deaths in New York City. 386 Since then, WNV has spread across epidemiology and transmission patterns are the subject of ongoing study: an Ehrlichia muris-like agent in Minnesota and Wisconsin 441 and the Heartland virus in Missouri. 442 Based on small numbers of patients, the Ehrlichia muris-like agent appears to cause fever, malaise, fatigue, headache, nausea, and vomiting, whereas the Heartland virus is associated with a flulike illness, with fever, fatigue, loss of appetite, and diarrhea. Like the mosquito-borne Rift Valley fever virus, the Heartland virus is a phlebovirus, a genus of the Bunyaviridae family of negative-stranded, enveloped RNA viruses. It is the first tick-borne phlebovirus known to cause human disease in the Americas.

Another novel phlebovirus-also thought to be tick-borne-was recently reported in China as the cause of an outbreak of severe fever with thrombocytopenia syndrome, with a case-fatality rate of 12% (171 confirmed cases and 21 deaths). 443, 444 The severe fever with thrombocytopenia syndrome virus, which can cause fever, vomiting, diarrhea, and multiple organ failure, may be transmitted to humans by Haema physalis longicornis ticks carried by domestic animals 443, 444 and may also spread from person to person through direct contact with infected blood or mucus. [445] [446] [447] 

Although the introduction of antibiotics in the early to mid-20th century remains one of the most significant health achievements to date, antimicrobial resistance is regarded as one of the greatest threats to human health worldwide. [448] [449] [450] 451, 452, 453 The widespread availability and use of antimicrobial agents among humans and animals, along with a globalized society in which emerging resistant strains can quickly spread worldwide, has created an environment of antibiotic exposure thus intensifying selective pressure and boosting the inherent capacity of microbes to develop resistance genes. Each year, antibioticresistant infections cost the U.S. health care system more than $20 billion and are responsible for more than 8 million additional hospital days. 454, 455 Moreover, these infections often require the use of less effective, more expensive, and often more toxic drugs. Areas of particular concern include continued threats from multi-and pan-resistant strains of Mycobacterium tuberculosis, health care-and communityassociated methicillin-resistant Staphylococcus aureus (MRSA) infections with continued evolution and global spread of highly virulent clones, along with newer challenges including increases in highly resistant gram-negative bacteria, particularly carbapenem-resistant Enterobacteriaceae and increasing resistance to third-generation cephalosporins in persons infected with Neisseria gonorrhoeae. 456 Also of concern are increases in severe, life-threatening C. difficile infections, usually associated with recent antibiotic use and often occurring among hospitalized patients (see "Diarrheal Disease"). As conduit and amplifier of antimicrobial resistance, health care settings are critical to its control.

The evolving nature of microbes defines antimicrobial resistance. Resistance genes have been found in humans and animals in areas with limited to no antibiotic exposure, in bacterial DNA frozen in the Arctic for 30,000 years, and in bacteria from underground caves isolated for 4 million years, some of which were resistant to synthetic antibiotics developed in the 20th century. [457] [458] [459] Enhancing this evolutionary process is antibiotic exposure. After introduction of each new class of antibiotics has come global spread of resistant strains, with factors such as global travel and trade accelerating this spread (Fig. 14-10 ). As early as the 1930s, sulfonamide-resistant strains of Streptococcus pyogenes were noted in military hospitals, and numerous bacterial strains, including S. aureus, were found to be resistant to penicillin shortly after its introduction and use in the 1940s. 460, 461 In 1961, just 2 years after the introduction of methicillin, MRSA strains emerged in British hospitals, 462, 463 and strains exhibiting intermediate or high level resistance to vancomycin have been identified since 1996. 464, 465, 466, 467 Similar patterns of resistance have emerged in other important pathogens. For N. gonorrhoeae, resistance to sulfonamides was common by the 1940s, and penicillin-and tetracycline-resistant strains became widespread during the 1980s. 468 Fluoroquinolone-resistant strains emerged during the 1990s and 2000s and spread quickly, leaving cephalosporins as the only remaining antimicrobial agents for treatment of gonococcal infections. 469 In recent years, the emergence of strains with decreased susceptibility to cephalosporins has threatened a The causative agents of Ebola and Marburg hemorrhagic fevers are filoviruses, belonging to the Filoviridae. These viruses are associated with fruit bats, which may be their natural animal reservoirs. [409] [410] [411] [412] Marburg virus was detected first, in 1967, when 31 cases (7 fatal) occurred in Germany and Yugoslavia among laboratory workers handling tissues from African green monkeys. 413 Eight years later, in 1975, a traveler returning from Rhodesia (now Zimbabwe) died in a hospital in Johannesburg, South Africa; his traveling companion and a nurse subsequently became ill, although both survived. 414 During the 1980s, two cases of Marburg hemorrhagic fever were reported in visitors to Kitum Cave in Mount Elgon National Park, Kenya. 415, 416 Ebola virus was first detected in 1976 as the cause of outbreaks with high fatality rates in Zaire (now the Democratic Republic of the Congo) 417 and the Sudan. 418 The Ebola strains associated with the 1976 outbreaks were named Ebola-Zaire and Ebola-Sudan. Other Ebola strains that cause human disease include Ebola-Ivory Coast (isolated in 1994 when a scientist became ill after conducting an autopsy on a chimpanzee from the Tai Forest) 419 and Ebola-Bundibugyo (isolated in 2007 during an outbreak in Uganda). 420 A case of Ebola-Sudan was reported in the United Kingdom in 1976 in a laboratory worker infected via the accidental stick of a contaminated needle, 421 and a case of Ebola-Zaire was reported in South Africa in 1996 in a physician who had traveled to Johannesburg after treating Ebola virus-infected patients in Gabon (the site of three Ebola outbreaks during the 1990s). The physician survived, but a nurse who took care of him became infected and died. 422 A fifth strain of Ebola was identified in Reston, Virginia, as the cause of severe illness and death in Philippine monkeys imported by research facilities in the United States in 1989 and 1990 423, 424 and Italy in 1992. 425 In 2008, Ebola-Reston was detected in pigs on two farms in the Philippines. 426 Six workers from the pig farm and from a slaughterhouse developed antibodies but did not become ill.

Outbreaks of Ebola have recurred multiple times in central and East Africa over the past decade, in the Republic of the Congo in 2003, 427, 428 in the Sudan in 2004, 429 345, 431 Although viral hemorrhagic fever outbreaks have lasted from a few months to more than a year, the Ebola and Marburg outbreaks that occurred in Uganda in 2012 were contained within 3 weeks. Hemorrhagic fever outbreaks typically result from a single or small number of spillover events from the virus reservoir with subsequent chains of human-to-human transmission in community (sometimes associated with funerals) and hospital settings. 435 Transmission in health care settings can be prevented by adherence to basic infection control practices and proper disposal of potentially infectious items. 436 The four distinct filovirus outbreaks that occurred in Uganda and the Democratic Republic of the Congo in 2012 were quickly identified and brought under control. That these outbreaks were kept relatively small is attributable in part to the availability of in-country filovirus diagnostics at the viral hemorrhagic fever reference laboratory located at the Uganda Viral Research Institute (UVRI) in Entebbe. This laboratory is a component of a viral hemorrhagic fever surveillance program established in 2010 by the UVRI and the Uganda Ministry of Health, in collaboration with the CDC. 345, 431 Current challenges include improving regional disease surveillance, developing additional diagnostic tools to assist in early diagnosis, and conducting ecologic investigations of Ebola and Marburg viruses. A better understanding of the natural reservoirs of these viruses and how they are spread may help prevent future outbreaks.

Public health concern about tick-borne diseases has increased in the United States, owing to the geographic spread of Lyme disease (along with its vector, the black-legged tick, Ixodes scapularis), [437] [438] [439] and the discovery of a new vector (the brown dog tick, Rhipicephalus san guineus) for Rocky Mountain spotted fever, identified during the investigation of outbreaks in Arizona. 440 Moreover, two new tickborne pathogens have been identified in the United States whose development have escalated bacterial antibiotic resistance to crisis levels and garnered the attention of public health, clinical, and policy leaders worldwide.

Efforts to reduce antimicrobial resistance have largely focused on surveillance and infection control in health care settings, along with educational campaigns targeted to health care providers and consumers on judicious antimicrobial use, such as the CDC's "Get Smart: Know When Antibiotics Work" campaign. 483 In recent years, impressive gains have been made by U.S. health care settings in reducing several types of HAIs, many of which are drug resistant. 484 As an example, invasive hospital-acquired MRSA infections declined 28% from 2005 through 2008 485 and MRSA bloodstream infections in hospitals decreased nearly 50% between 1997 and 2007. 486 These and other declines in hospital-acquired infections followed targeted, multifaceted efforts undertaken to increase adherence to recommended infection control practices, educate patients and providers, issue facility-specific guidelines for prescription and use of antimicrobial agents, and implement appropriate isolation and cohorting of patients infected or colonized with drug-resistant organisms. In addition, hospital-acquired infections are reported and tracked by the Centers for Medicare and Medicaid Services (CMS) and through reporting mandates in many states using the CDC's National Healthcare Safety Network (NHSN). This secure, Internet-based surveillance system collects data from more than 12,000 facilities in all 50 states on hospital-acquired infections and related issues, including the incidence or prevalence of multidrug-resistant organisms, health care personnel safety and vaccination, and the occurrence of transfusion-related adverse events. Reviews of antibiotic stewardship programs in both large and small hospitals have documented their effectiveness, with facility-specific reductions in antibiotic use of 22% to 36% and related annual cost savings of $200,000 to $900,000. 487, 488 With 50% of antibiotic prescriptions estimated to be unnecessary, 489 education of patients and providers on the importance of and health benefits from responsible use of antibiotics remains paramount.

Use of antibiotics as growth promoters in healthy food-producing animals is also a significant concern. Globally, the amount of antibiotics used in food-producing animals surpasses the amount of antibiotics used to treat human disease. 450 The human health implications of this return to potentially untreatable gonorrhea and prompted changes in U.S. gonorrhea treatment guidelines to prolong the effectiveness of these drugs. 470, 471 Over the past 2 decades, highly drug-resistant strains of M. tuberculosis have also emerged worldwide, 472 including multidrug-resistant tuberculosis (defined as tuberculosis that is resistant to isoniazid and rifampin, the two most effective first-line tuberculosis drugs) and extensively drug-resistant tuberculosis (defined as multidrug-resistant tuberculosis that is also resistant to any fluoroquinolone drug and at least one of three second-line injectable drugs: amikacin, kanamycin, or capreomycin). A survey of more than 25 international reference laboratories conducted by the WHO and CDC found that during 2000 to 2004, among 17,690 M. tuberculosis isolates, 20% were multidrug resistant and 2% were extensively drug resistant. 473 Although tuberculosis cases in the United States continue to decline, an estimated one third of the world's population is infected with M. tuberculosis, and each year approximately 9 million people develop tubercular disease and 2 million die from tuberculosis-related deaths. 474 These infections present serious public health challenges and raise the specter of virtually untreatable tuberculosis outbreaks. 475 Carbapenem-resistant Enterobacteriaceae are also particularly alarming, with some showing resistance to all available antibiotics. 476, 477 These infections are primarily transmitted in health care settings and can be severe, with mortality rates of 40% to 50%. [478] [479] [480] Patients who require prolonged hospitalization and critically ill patients exposed to invasive medical devices (e.g., ventilators and central venous catheters) are at special risk. Resistance to carbapenem antibiotics is mediated through the production of carbapenemases (enzymes that inactivate carbapenems), including Klebsiella pneumoniae carbapenemase (KPC), first identified in 2001 in the United States, 481 and New Delhi β-lactamase (NDM), first identified in 2008 in India. 482 The genes encoding KPC and NDM (carried on plasmids) have spread from K. pneumoniae to other gram-negative bacteria, including E. coli, Klebsi ella species, and Enterobacter species.

The problem of antimicrobial resistance extends beyond bacteria and includes many priority viral (e.g., HIV, influenza), fungal (e.g., Candida, Aspergillus), and parasitic (e.g., malaria) infections. However, the increasing use of antibiotics in both humans and animals, the spread of HAIs into communities, and a virtual standstill in antibiotic Methicillin (e.g., fecal transplants to treat C. difficile) also offer tremendous promise for reducing antimicrobial resistance. Vaccines represent the optimal solution to addressing infections and antimicrobial resistance, and research and development of new vaccines is an urgent need. Effective immunization programs have stopped emergence of resistant strains and precluded the need for new antimicrobial agents for multiple infectious diseases, allowing focus to be shifted to diseases for which drug resistance remains a major global health threat. A recent example of this impact includes the pneumococcal conjugate vaccine. Over the past several years, use of this vaccine not only has reduced the rate of disease but also has decreased antibiotic resistance by targeting pneumococcal strains that are most often resistant. [499] [500] [501] 

The cross-border spread of infectious diseases, the upsurge in newly identified infections along with the emergence of known infections in new geographic regions, the unrelenting evolution of resistant organisms, and continued concerns about bioterrorism serve as compelling reminders of the importance of ensuring strong and sustainable clinical, public health, and laboratory capacity and collaborations at the local, national, and international levels. 502 These fundamental elements can help create globally linked surveillance and laboratory systems that can facilitate rapid recognition of and response to infectious disease events. 503, 504 The World Health Organization has long played a major role in establishing and supporting such global health collaborations. A primary example is the Global Influenza Surveillance and Response System (GISRS) (Fig. 14-11 ), in operation for more than 6 decades. 505 Formerly known as the Global Influenza Surveillance Network, GISRS currently comprises six WHO collaborating centers, four WHO Essential Regulatory Laboratories, and 141 institutions across 111 countries (WHO member states). Responsibilities include monitoring the evolution of influenza viruses and providing recommendations on laboratory diagnostics, vaccine development, and risk assessment. Also critical is WHO's Global Outbreak Alert and Response Network (GOARN), 506 a collaboration of existing networks and institutions use are increasingly being recognized. A recent study comparing workers in industrial and antibiotic-free livestock operations found livestock-associated MRSA and multidrug-resistant S. aureus carriage only among the industrial workers. 490 Linkages have also been found between E. coli isolates from retail meat (chicken) and extraintestinal pathogenic E. coli urinary tract infections in humans, potentially affecting treatment of these common infections. 491 Several nations have taken steps to address the nontherapeutic use of antibiotics in foodproducing animals, beginning with Sweden in 1986 and extending to the European Union in 2006, with bans on agricultural growth promoters. 492 The FDA strategy to promote the judicious use of antibiotics important in treating humans recommends that such antibiotics be used in food-producing animals only under veterinary oversight and only to address animal health needs, not to promote growth. 493 For antimicrobial drug development, the outlook is grim. Despite the continued rise in antibiotic-resistant pathogens, the development of new antibiotics has dramatically slowed. For the 5-year period 1983 to 1987, 16 new systemic antibiotics were approved for use in humans by the FDA; from 2008 to 2012, only 2 were approved. 494 Particularly troubling, there have been no new classes of drugs to treat gramnegative bacteria in 4 decades. 488 With the discovery of new antimicrobial agents more scientifically challenging compared with earlier years, unfavorable profit margins from short-course therapies, and other disincentives, many pharmaceutical companies have abandoned the market. Several U.S. policy steps have been taken to counteract these effects, including the FDA Safety and Innovation Act, 495 passed into law in 2012, which seeks to increase the development of and patient access to new antimicrobial agents.

More recent efforts have focused on the use of new technologies and host targets to better understand and reduce antimicrobial resistance. Whole-genome sequencing techniques have been used to track bacterial transmission from person to person and to better define and determine transmission linkages in outbreaks of resistant HAIs. [496] [497] [498] As these technologies continue to advance, rapid, point-of-care diagnostic tests could be used to quickly identify resistant infections and target treatment. Efforts to prevent and control infectious diseases by altering the host-microbe interaction as opposed to targeting microbes FIGURE parasitic disease drug service regarding requests from physicians in New York and California for pentamidine isethionate for treatment of Pneumocystis carinii (now Pneumocystis jirovecii) pneumonia in patients with no known cause of immunodeficiency hinted at the first U.S. cases of AIDS. 510 Recognition of unusually severe respiratory disease by observant clinicians signaled hantavirus pulmonary syndrome in 1993, and suspicion of anthrax by alert clinical and laboratory staff in Florida in 2001 suggested a possible bioterrorist event. A February 10, 2003, email posted by a physician on ProMED, an informal online infectious disease reporting program of the International Society for Infectious Diseases, is widely regarded as the first notification of the global outbreak of SARS. 511 Such observations have not been limited to the medical and scientific community, however. In the mid-1970s, two Connecticut mothers questioning what was believed to be an unusually large number of juvenile rheumatoid arthritis cases in their community led researchers to the discovery of Lyme disease and a concerned American Legion official provided the first indication to health authorities of the emergence of legionnaires' disease. 512 Although today's globalized world has created a perfect environment for rapid emergence and spread of infectious diseases, it has also brought significant scientific, technologic, and communication advances for their control. Next-generation sequencing technologies and expanded bioinformatics capacities are revolutionizing the field of microbiology, reducing the amount of time needed for pathogen detection and analysis and generating data for a more detailed understanding of infectious agents. 503, 513 These tools offer new opportunities to improve public health efforts to detect and control outbreaks, determine antimicrobial susceptibility, and develop and target vaccines. 313, 314, 497, 498 Scientists are also gaining new understanding of the role of the microbiome and microbial sensors in infectious diseases, 514, 515 offering new insight into pathogen-host complexities and disease treatment and prevention. Continued advances in electronic communications are facilitating earlier recognition of emerging problems and rapid exchange of information. In particular, early warning systems such as ProMED-mail, the Public Health Agency of Canada's Global Public Health Intelligence Network (GPHIN), and HealthMap, collect, categorize, and display outbreak and disease information from a variety of formal and informal sources-enhancing disease surveillance and tracking capabilities. [516] [517] [518] Expansions in Internet access and use and far-reaching social media networks have also increased the exchange of health information and broadened public health partnerships to include nontraditional partners such as law enforcement, the media, and members of the public at local, national, and global levels.

Whereas focus on emerging and reemerging infections is paramount, requiring ongoing vigilance and a globally linked infrastructure, priority attention must remain on reducing high-burden infections that account for the majority of disease and disability caused by microbial agents. 5, 6 These efforts should ideally work in tandem, enabling rapid recognition and effective response to emerging infections and other public health emergencies along with implementation of new and proven measures to reduce the burden of endemic infectious diseases and advance global health equity. established in 2000 to address threats from epidemic-prone and emerging infectious threats. GOARN provides an operational framework to ensure the availability of skills, expertise, and resources needed to keep the international community aware of and ready to respond to potential outbreaks. 507 In the aftermath of the 2003 SARS outbreak, the global public health community completed work on new International Health Regulations (IHR), an international treaty that gives the WHO authority over and places requirements on its member states for detecting, reporting, and controlling infectious diseases. 508 Adopted by the World Health Assembly in 2005 and made effective in 2007, the new regulations require prompt reporting of all public health emergencies of international concern, expanding beyond infectious diseases to include events resulting from biological, chemical, or radionuclear threats as well as natural disasters. In addition to reporting of public health emergencies of international concern, the regulations require member states to notify the WHO of a single case of smallpox; poliomyelitis due to wild-type poliovirus; SARS; and human influenza caused by a new subtype. With the goal of controlling health threats at the local level, the new regulations allow for the use of surveillance information beyond official state notification and have established new requirements for member states to support existing global surveillance and response systems and to develop proactive systems for strengthening national and international capacities.

Significant contributions to global health and infectious disease control have also been made through private-sector support. A leading example is the Bill and Melinda Gates Foundation (www .gatesfoundation.org), a privately funded effort begun in 2000 focused on reducing health disparities across the world and helping to address the global health challenges outlined by the 2000 Millennium Development Goals (www.un.org/millenniumgoals). Priorities for the Gates Foundation include efforts to reduce HIV infection/AIDS, malaria, tuberculosis, vaccine-preventable diseases, diarrheal diseases, pneumonia, and neglected infectious diseases. Other important global health initiatives supported by both government and nongovernment donors include the Global Alliance for Vaccines and Immunizations (GAVI Alliance; www.gavialliance.org) and the Global Fund to Fight AIDS, Tuberculosis, and Malaria (www.theglobalfund.org). Two large U.S.-led efforts, the President's Malaria Initiative (PMI; www .fightingmalaria.gov) and the President's Emergency Plan for AIDS Relief (PEPFAR; www.pepfar.gov) provide targeted prevention and treatment support in countries most heavily affected by these leading killers. In particular, the impact of PEPFAR has been dramatic. Begun in 2004, the program requires country ownership and results-based efforts with goals for sustainability. In 2011 alone, PEPFAR supported treatment of nearly 4 million people, supported antiretroviral therapy for more than 1.5 HIV-infected pregnant women to prevent perinatal infection, and supported diagnostic testing for more than 40 million individuals. 509 Although national and global partnerships are critical to controlling infectious disease threats, local clinical, public health, and laboratory capacity remains the cornerstone for initial disease recognition and response. In the early 1980s, concerns by staff in the CDC's

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Increasing rates of vibriosis in the United States, 1996-2010: review of surveillance data from 2 systems

A review of human salmonellosis, III. Magnitude of Salmonella infection in the United States

Clostridium diffi cile infection in patients discharged from US short-stay hospitals

Concern over Clostridium difficile in the USA

Current status of Clos tridium difficile infection epidemiology

Emergence and global spread of epidemic healthcare-associated Clostridium dif ficile

The epidemiology of Clostridium difficile infection in children: a population-based study

An epidemic, toxin gene-variant strain of Clostridium difficile

A predominantly clonal multi-institutional outbreak of Clostridium difficileassociated diarrhea with high morbidity and mortality

A portrait of the geographic dissemination of the Clostridium difficile North American pulsed-field type 1 strain and the epidemiology of C. difficile-associated disease in Quebec

Relationship between bacterial strain type, host biomarkers, and mortality in Clostridium difficile infection

Emergence of Clos tridium difficile infection due to a new hypervirulent strain, polymerase chain reaction ribotype 078

Clos tridium difficile in retail ground meat

Treating Clostridium difficile infection with fecal microbiota transplantation

Duodenal infusion of donor feces for recurrent Clostridium difficile

The growing threat of foodborne bacterial enteropathogens of animal origin

Emergence of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 infections in the United States

International Salmonella Typhimurium DT104 infections

Antimicrobialresistant nontyphoidal Salmonella is associated with excess bloodstream infections and hospitalizations

Quinolone-resistant Campylobacter jejuni infections in Minnesota, 1992-1998

Antimicrobial susceptibility patterns of Shigella isolates in Foodborne Diseases Active Surveillance Network (FoodNet) sites

Outbreak of infections caused by Shigella sonnei with decreased susceptibility to azithromycin

Rotavirus vaccines: development, current issues, and future prospects

World Health Organization. Rotavirus vaccine. WHO position paper

An outbreak of cholera in Maryland associated with imported commercial frozen fresh coconut milk

Multistate Outbreak of Human Salmonella enteritidis Infections Linked to Turkish Pine Nuts

GeoSentinel surveillance of illness in returned travelers

Enteropathogens and chronic illness in returning travelers

Invasive nontyphoidal salmonella disease: an emerging and neglected tropical disease in Africa

Intracontinental spread of human invasive Salmonella pathovariants in sub-Saharan Africa

Outbreak of Vibrio parahaemolyticus gastroenteritis associated with Alaskan oysters

Promising new assays and technologies for the diagnosis and management of infectious diseases

From molecular to genomic epidemiology: transforming surveillance and control of infectious diseases

Impacts of culture-independent diagnostic practices on public health surveillance for bacterial enteric pathogens

Metagenomics, infectious disease diagnostics, and outbreak investigations: sequence first, ask questions later?

A national outbreak of Salmonella serotype Tennessee infections from contaminated peanut butter: a new food vehicle for salmonellosis in the United States

Salmonella typhimurium infections associated with peanut products

Salmonella Bredeney infections linked to a brand of peanut butter-United States

Extendedspectrum β-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors

Enterotoxigenic Bacte roides fragilis-associated diarrhea in children 0-2 years of age in rural Bangladesh

Severe diarrhea caused by cholera toxin-producing Vibrio cholerae serogroup O75 infections acquired in the Southeastern United States

Toxigenic Vibrio cholerae serogroup O141-associated cholera-like diarrhea and bloodstream infection in the United States

A newly identified bocavirus species in human stool

Role of Klebsiella oxytoca in antibiotic-associated diarrhea

Klebsiella oxytoca as a causative organism of antibiotic-associated hemorrhagic colitis

Enterohemorrhagic Escherichia coli O26:H11/H−: a new virulent clone emerges in Europe

A large outbreak of Brainerd diarrhea associated with a restaurant in the Red River Valley

A restaurant-associated outbreak of Brainerd diarrhea in California

Nosocomial diarrhea: evaluation and treatment of causes other than Clostridium difficile

Unpasteurized milk: a continued public health threat

Outbreak of multidrug-resistant Salmonella enterica serotype Newport infections associated with consumption of unpasteurized Mexican-style aged cheese-Illinois

Campylo bacter jejuni infection associated with unpasteurized milk and cheese-Kansas

Enhanced susceptibility to Salmo nella infection in streptomycin-treated mice

Massive outbreak of antimicrobial resistant salmonellosis traced to pasteurized milk

Antibiotic use among children in an urban Brazilian slum: a risk factor for diarrhea?

Estimates of enteric illness attributable to contact with animals and their environments in the United States

Multistate outbreak of Salmonella Infantis, Newport, and Lille infections linked to live poultry from a single mail-order hatchery in Ohio

Outbreak of salmonellosis linked to live poultry from a mailorder hatchery

Multistate outbreak of human Salmonella typhimurium infections linked to contact with pet hedgehogs-United States

US outbreak of human Salmonella infections associated with aquatic frogs

Risk factors for human disease emergence

Origin of HIV-1 in the chimpanzee Pan troglodytes troglodytes

Rift Valley fever virus

Bats and emerging zoonoses: henipaviruses and SARS

Zoonotic poxviruses

Genomic analysis of filoviruses associated with four viral hemorrhagic fever outbreaks in Uganda and the Democratic Republic of the Congo in 2012

Avian influenza: update

Emerging risk of H7N9 influenza in China

(H3N2) variant virus-related hospitalizations: Ohio

Executive summary of the AVMA One Health Initiative Task Force report

Ecology of zoonoses: natural and unnatural histories

Breaking the chain: Rift Valley fever virus control via livestock vaccination

Prediction and prevention of the next pandemic zoonosis

Drivers, dynamics, and control of emerging vector-borne zoonotic diseases

Epidemic dengue/dengue hemorrhagic fever as a public health, social, and economic problem in the 21st century

The global distribution and burden of dengue

Dengue virus infection: epidemiology, pathogenesis, clinical presentation, diagnosis, and prevention

Using disability-adjusted life years to assess the economic impact of dengue in Puerto Rico: 1984-1994

Burden of symptomatic dengue infection in children at primary school in Thailand: a prospective study

The history of dengue outbreaks in the Americas

Centers for Disease Control and Prevention. Notes from the field: dengue epidemic-Puerto Rico

Dengue virus infection in Africa

Dengue deaths in Puerto Rico: lessons learned from the 2007 epidemic

Progress towards a dengue vaccine

Protective efficacy of the recombinant, live attenuated, CYD tetravalent dengue vaccine in Thai schoolchildren: a randomised, controlled phase 2b trial

World Health Organization. Chikungunya and Dengue in the Southwest Indian Ocean. Global Alert and Response

Chikungunya: a reemerging virus

Chikungunya in Europe: what's next?

Pan American Health Organization. Preparedness and Response for Chikungunya Virus: Introduction in the Ameri cas

Chikungunya disease outbreak, Réunion Island. Emerg Infect Dis

Chikungunya fever

Infection with chikungunya virus in Italy: an outbreak in a temperate region

Seasonality of mosquitoes and chikungunya in Italy

Chikungunya virus adapts to tiger mosquito via evolutionary convergence: a sign of things to come?

Chikungunya virus and prospects for a vaccine

A chimeric vesiculo/alphavirus is an effective alphavirus vaccine

A recombinant measles vaccine expressing chikungunya virus-like particles is strongly immunogenic and protects mice from lethal challenge with chikungunya virus

Chikungunya virus: an update on antiviral development and challenges

West Nile virus: a primer for the clinician

West Nile virus

West Nile encephalitis epidemic in southeastern Romania

Outbreak of West Nile virus infection

West Nile fever outbreak, Israel, 2000: epidemiologic aspects

The outbreak of West Nile virus infection in the New York City area

West Nile virus surveillance and diagnostics: a Canadian perspective

Estimated cumulative incidence of West Nile virus infection in US adults

West Nile virus: review of the literature

The continuing spread of West Nile virus in the Western Hemisphere

West Nile virus in the United States: guidelines for detection, prevention, and control

West Nile virus neuroinvasive disease

A global perspective on the epidemiology of West Nile virus

West Nile Virus Disease Cases and Deaths Reported to CDC by Year and Clinical Presentation

West Nile virus

Transmission of West Nile virus through blood transfusion in the United States in 2002

Transmission of West Nile virus from an organ donor to four transplant recipients

Experimental infection of North American birds with the New York 1999 strain of West Nile virus

Neurologic manifestations and outcome of West Nile virus infection

West Nile virusassociated flaccid paralysis outcome

Phylogenetic analysis of North American West Nile virus isolates

Continued evolution of West Nile virus

Unpredictable and difficult to control-the adolescence of West Nile virus

Phase II, dose ranging study of the safety and immunogenicity of single dose West Nile vaccine in healthy adults ≥ 50 years of age

Clinical management of filovirus-infected patients

Fruit bats as reservoirs of Ebola virus

Human Ebola outbreak resulting from direct exposure to fruit bats in Luebo, Democratic Republic of Congo

Marburg virus infection detected in a common African bat

Studies of reservoir hosts for Marburg virus

Marburg virus" disease): a new zoonosis

Epidemiologic investigation of Marburg virus disease

Characterization of a new Marburg virus isolated from a 1987 fatal case in Kenya

Marburg-virus disease in Kenya

World Health Organization. Ebola haemorrhagic fever in Zaire

World Health Organization. Ebola haemorrhagic fever in Sudan

Isolation and partial characterisation of a new strain of Ebola virus

Newly discovered Ebola virus associated with hemorrhagic fever outbreak in Uganda

A case of Ebola virus infection

Fears over Ebola spread as nurse dies

Isolated cases of Ebola (subtype Reston) virus among quarantined non-human primates recently imported from the Philippines to the United States

Update: filovirus infection in animal handlers

World Health Organization. Viral hemorrhagic fever in imported monkeys

Epidemiology of Ebola (subtype Reston) virus in the Philippines

Outbreak of Ebola hemorrhagic fever in the Republic of the Congo, 2003: a new strategy

World Health Organization. Ebola Haemorrhagic Fever in the Republic of the Congo-Update 6. Global Alert and Response

Laboratory diagnosis of Ebola hemorrhagic fever during an outbreak in Yambio, Sudan

End of Ebola Outbreak in the Democratic Republic of the Congo. Global Alert and Response

Repeated outbreaks of viral hemorrhagic fevers in Uganda

Marburg hemorrhagic fever associated with multiple genetic lineages of virus

Marburg virus genomics and association with a large hemorrhagic fever outbreak in Angola

Outbreak of Marburg hemorrhagic fever among miners in Kamwenge and Ibanda Districts, Uganda

Filovirus outbreak detection and surveillance: lessons from Bundibugyo

World Health Organization. Interim Infection Control Recommendations for Care of Patients with Suspected or Confirmed Filovirus (Ebola, Marburg) Haemorrhagic Fever

Phylogeography of Borrelia burgdorferi in the eastern United States reflects multiple independent Lyme disease emergence events

Invasion of the Lyme disease vector Ixodes scapularis: implications for Borrelia burgdorferi endemicity

Human risk of infection with Borrelia burgdorferi, the Lyme disease agent, in eastern United States

Rocky Mountain spotted fever from an unexpected tick vector in Arizona

Emergence of a new pathogenic Ehrlichia species, Wisconsin and Minnesota

A new phlebovirus associated with severe febrile illness in Missouri

Fever with thrombocytopenia associated with a novel bunyavirus in China

Severe fever with thrombocytopenia syndrome virus

A family cluster of infections by a newly recognized bunyavirus in eastern China, 2007: further evidence of person-to-person transmission

Person-to-person transmission of severe fever with thrombocytopenia syndrome virus. Vector Borne Zoonotic Dis

Human-to-human transmission of severe fever with thrombocytopenia syndrome bunyavirus through contact with infectious blood

Eighth Edition: An Initia tive of the Risk Response Network

The 10 × '20 initiative: pursuing a global commitment to develop 10 new antibacterial drugs by 2020

World Health Organization. The Evolving Threat of Antimi crobial Resistance: Options for Action. Geneva: World Health Organization

Drug Resistance in Infectious Agents-a Global Threat to Humanity

Infectious Diseases Society of America. Combatting antimicrobial resistance: policy recommendations to save lives

European Centre for Disease Prevention and Control, European Medicines Agency

Hospital and societal costs of antimicrobial-resistant infections in a Chicago teaching hospital: implications for antibiotic stewardship

Antibiotic-Resistant Infections Cost the US Healthcare System in Excess of $20 Billion Annually

Alliance for the Prudent Use of Antibiotics and bioMérieux

Antibiotic Resistance Threats in the United States

The global spread of healthcare-associated multidrug-resistant bacteria: a perspective from Asia

Emergence of antibiotic resistance: need for a new paradigm

Antibiotic resistance is prevalent in an isolated cave microbiome

Microbial resistance to antibiotics: an evolving and persistent problem

Infection by penicillin-resistant staphylococci

Methicillin-resistant staphylococci

40 years of methicillin resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility

Emergence of vancomycin resistance in Staphylococcus aureus

Staphylococ cus aureus resistant to vancomycin-United States

Vancomycin-intermediate and -resistant Staphylococcus aureus: what the infectious disease specialist needs to know

Update to CDC's sexually transmitted diseases treatment guidelines, 2006: fluoroquinolones no longer recommended for treatment of gonococcal infections

The emerging threat of untreatable gonococcal infection

Update to CDC's sexually transmitted diseases treatment guidelines, 2010: oral cephalosporins no longer a recommended treatment for gonococcal infections

Worldwide emergence of extensively drug-resistant tuberculosis

Emergence of Mycobacterium tuberculosis with extensive resistance to second-line drugs-worldwide

Surveillance, Planning, Financing. Publication No. WHO/HTM/TB/2008.393. Geneva: World Health Organization

Plan to combat extensively drug-resistant tuberculosis: recommendations of the Federal Tuberculosis Task Force

Carbapenem-resistant Enterobacteriaceae: a potential threat

Global spread of carbapenemase-producing Enterobacteriaceae

Outcomes of carbapenem-resistant Klebsiella pneumoniae infection and the impact of antimicrobial and adjunctive therapies

Containment of a country-wide outbreak of carbapenem-resistant Klebsiella pneumoniae in Israeli hospitals via a nationally implemented intervention

Outbreak of carbapenem-resistant Enterobacteriaceae at a long-term acute care hospital: sustained reductions in transmission through active surveillance and targeted interventions

Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae

Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study

National and State Healthcare-Associated Infection Standardized Infection Ratio Report

Health care-associated invasive MRSA infections

Infectious Diseases Society of America and the Society for Healthcare Epidemiology of America guidelines for developing an institutional program to enhance antimicrobial stewardship

A call to arms: the imperative for antimicrobial stewardship

Office-related antibiotic prescribing for persons aged ≤14 years-United States

Livestock-associated methicillin and multidrug resistant Staphylococcus aureus is present among industrial workers, not antibiotic-free livestock operation workers in North Carolina

Chicken as reservoir for extraintestinal pathogenic Escherichia coli in humans

Restricting antimicrobial use in food animals: lessons from

Animal and Veterinary

New antibiotic development: barriers and opportunities in 2012. Alliance for the Prudent Use of Anti biotics Newsletter

Food and Drug Administration Safety and Innovation Act (FDASIA)

Whole-genome sequencing for analysis of an outbreak of methicillinresistant Staphylococcus aureus: a descriptive study

Tracking a hospital outbreak of carbapenem-resistant Klebsiella pneu moniae with whole-genome sequencing

Rapid wholegenome sequencing for investigation of a neonatal MRSA outbreak

Effect of introduction of the pneumococcal conjugate vaccine on drugresistant Streptococcus pneumoniae

Sustained reductions in invasive pneumococcal disease in the era of conjugate vaccine

hospitalizations for pneumonia after a decade of pneumococcal vaccination

for the Committee on Emerging Microbial Threats to Health in the 21st Century, Board on Global Health, Institute of Medicine. Microbial Threats to Health: Emergence, Detec tion, and Response

The changing face of pathogen discovery and surveillance

Global capacity for emerging infectious disease detection

World Health Organization. Global Influenza Surveillance and Response System (GISRS)

World Health Organization. Global Alert and Response. Global Outbreak Alert and Response Network

Hot spots in a wired world: WHO surveillance of emerging and re-emerging infectious diseases

World Health Organization. International Health Regulations

The United States President's Emergency Plan for AIDS Relief: a story of partnerships and smart investments to turn the tide of the global AIDS pandemic

Centers for Disease Control and Prevention

International Society for Infectious Diseases. ProMEDmail. Archive number 20030210.0357. February

How outbreaks of infectious disease are detected: a review of surveillance systems and outbreaks

Microbial genomics and infectious diseases

Interactions between the microbiota and the immune system

The genetics of innate immunity sensors and human disease

ProMED-mail: an early warning system for emerging diseases

The Global Public Health Intelligence Network and early warning outbreak detection: a Canadian contribution to global public health

HealthMap: global infectious disease monitoring through automated classification and visualization of internet media reports