key: cord-0077861-4ynip0nt
authors: Beekman, Rosalie R. A. L.; Duijkers, Ruud R.; Snijders, Dominic D.; van der Eerden, Menno M.; Kross, Martijn M.; Boersma, Wim W. G.
title: Validating a clinical prediction score for Legionella-related community acquired pneumonia
date: 2022-05-09
journal: BMC Infect Dis
DOI: 10.1186/s12879-022-07433-z
sha: 8f9a9c2251218965678f1ff2cda5ace75d963c5b
doc_id: 77861
cord_uid: 4ynip0nt

BACKGROUND: Legionella-related community acquired pneumonia (CAP) is a disease with an increasing incidence and a high mortality rate, especially if empirical antibiotic therapy is inadequate. Antibiotic treatment highly relies on clinical symptoms, although proven non-specific, because currently available diagnostic techniques provide insufficient accuracy for detecting Legionella CAP on admission. This study validates a diagnostic scoring system for detection of Legionella-related CAP, based on six items on admission (Legionella prediction score). METHODS: We included patients with Legionella-related CAP admitted to five large Dutch hospitals between 2006 and 2016. Controls were non-Legionella-related CAP patients. The following six conditions were rewarded one point if present: fever > 39.4 °C; dry cough; hyponatremia (sodium) < 133 mmol/L; lactate dehydrogenase (LDH) > 225 mmol/L; C-reactive protein (CRP) > 187 mg/L and platelet count < 171 × 10(9)/L. The accuracy of the prediction score was assessed by calculating the area under the curve (AUC) through logistic regression analysis. RESULTS: We included 131 cases and 160 controls. A score of 0 occurred in non-Legionella-related CAP patients only, a score of 5 and 6 in Legionella-related CAP patients only. A cut-off ≥ 4 resulted in a sensitivity of 58.8% and a specificity of 93.1%. The AUC was 0.89 (95% CI 0.86–0.93). The strongest predictors were elevated LDH, elevated CRP and hyponatremia. CONCLUSIONS: This multi-centre study validates the Legionella prediction score, an easily applicable diagnostic scoring system, in a large group of patients and finds high diagnostic accuracy. The score shows promise for future prospective validation and could contribute to targeted antibiotic treatment of suspected Legionella CAP.

Legionella infection is an important cause of communityacquired pneumonia (CAP) with a mortality of 8-12% [1] . The average incidence of Legionella infection in CAP was reported 2.1-3.6% in a recent meta-analysis [2] . However, due to underdiagnosis, the true incidence is probably higher. In the USA, the incidence of reported Legionella cases quadrupled over the past decades [3] [4] [5] [6] . Legionella-related pneumonia has an overall higher burden of morbidity and mortality than other causes of CAP, especially if initial empirical antibiotic therapy is inadequate [3, 5, 7, 8] .

Diagnosis of Legionella-related CAP is difficult, because culturing Legionella from sputum and blood takes 3 to 10 days and has a low yield. The introduction of the urinary antigen test (UAT) for Legionella pneumophila improved diagnosis, especially in severe cases. However, the UAT can be negative in the early phase of the disease, especially in patients with mild disease. UAT detects only Legionella pneumophila serogroup 1 antigens, accounting for more than 80% of Legionella cases [9] [10] [11] . Over the last years several other subspecies of Legionella have been associated with significant clinical disease, especially Legionella longbeachae which is predominantly found in Australia, New Zealand and Asia, but recently has been detected in both USA and Europe [12] [13] [14] .

To prevent overuse of macrolides and quinolones, international guidelines recommend empirical antibiotic coverage of Legionella only when this infection is suspected based on clinical signs and symptoms, or in patients with severe CAP. Clinical scoring systems were developed to predict Legionella-related pneumonia, but most have limited clinical significance because of low accuracy or the need to include follow-up data over several days [15] [16] [17] . As a result initial empirical coverage may be inadequate [18] [19] [20] .

Fiumefreddo et al. [21] developed a diagnostic scoring system consisting of 6 items which are easily obtainable on admission, namely fever, dry cough, hyponatremia, elevated lactate dehydrogenase (LDH) and elevated C-reactive protein (CRP), further called: Legionella prediction score. In the derivation cohort, the diagnostic accuracy of the score was high, with an area under the curve (AUC) of 0.86 (95% confidence interval (CI) 0.81-0.90) [21] . In a previous validation study with 37 cases, the Legionella prediction score discriminated reasonably well between Legionella-related CAP and CAP caused by other pathogens (specificity 92% and sensitivity 31% at cut off ≥ 4, area under the curve 0.91) [22] .

In theory this prediction score could be a useful clinical tool to limit antibiotic overuse in selected patients, especially in cases that are not detected by UAT, due to a false-negative result, when UAT takes too long or it has not been performed. Therefore, we evaluated the performance of this score through external validation in a large, Dutch cohort of hospital-admitted patients with Legionella-related CAP.

In this cross-sectional, observational, retrospective study, data was collected from four large teaching hospitals and one University hospital in the Netherlands. A list of all patients tested positive with Legionella species between 2006 and 2016 was provided by the departments of microbiology. Medical records of all patients were reviewed and data was collected anonymously. Cases had at least one microbiological test positive for Legionella species, either culture, serology, PCR or UAT and consolidation(s) of the chest X-ray. UATs were performed with the BinaxNOW S. pneumoniae Antigen Card (Abbott). Our control group consisted of non-Legionella CAP-patients who required hospital admission, through random selection of participants from the REDUCE study, which was conducted in one of the previously mentioned teaching hospitals from 2013-2017 (full study protocol available via clinicaltrials.gov, NCT01964495). All patients included in the study had at least one consolidation on the chest X-ray together with clinical signs and symptoms indicative for CAP. Other inclusion criteria were a (pre-event) life expectancy of minimum 30 days and granted informed consent. Patients were excluded if they were pregnant or breastfeeding, if they had immunodeficiency (known immunodeficiency or receiving corticosteroids equivalent to 10 mg prednisolone per day), cancer, in case of obstruction, aspiration or hospital acquired pneumonia and if they were unable or unlikely to comprehend and follow the REDUCE protocol. Patients were included in the analysis if the items needed to calculate the Legionella prediction score were present. When available, causative pathogens for non-Legionella CAP were reported. Causative pathogens were defined as pathogens known to cause CAP, that were isolated from blood cultures, sputum cultures of good quality sputum (predominant leukocytes without squamous epithelial cells), urinary antigen tests and/or PCR. We retrospectively collected data from patient files which were anonymized for our study before processing the data. Therefore, in accordance to the current guidelines at that time in the Netherlands, informed consent was not required, neither was approval by a local ethics committee. This conforms to the Law regarding Medical Research involving human subjects in the Netherlands.

The collected data included vital parameters, clinical signs, laboratory findings, relevant comorbidities, smoking history and CURB-65 score. All data was obtained during the emergency care department visit (further called: on admission). The Legionella prediction score, ranging from 0-6, was calculated. For the six following conditions, if present, one point was scored: fever > 39.4 °C; dry cough; hyponatremia (sodium) < 133 mmol/L; LDH > 225 mmol/L; high CRP > 187 mg/L and low platelet count < 171 × 10 9 /L [21] .

The primary outcome was the diagnostic accuracy of the Legionella prediction score for Legionella-related CAP. Furthermore, we assessed the predictive value of the original, continuous parameters and the proposed cut-off points, both univariate and multivariate.

According to Toll et al., for validation of a prediction rule with a dichotomous outcomes, at least 100 cases and 100 controls are needed [23] . We estimated that a random selection of 185 REDUCE participants would provide sufficient controls. Patient characteristics were assessed for normal distribution with the Kolmogorov-Smirnov test. Either mean, standard deviation and Chi-square test or median, percentiles and Mann-Whitney-U test were reported.

Continuous parameters were analysed in a logistic regression model that was performed for each individual parameter and for all parameters combined. Thereafter, parameters were dichotomized in the categories used in the prediction score. Univariate and multivariate logistic regression was repeated with these dichotomized parameters.

For each regression, the b-coefficient, the odds ratio, AUC and p were calculated. A p-value < 0.05 was considered significant. Sensitivity, specificity, positive and negative predictive values were calculated for scores 0-6 of the prediction score.

To further assess accuracy, chi-square, the loglikelihood ratio and Nagelkerke square were calculated. IBM SPSS Statistics version 25.0 was used for all analyses.

We identified 252 patients with Legionella-related CAP and 185 patients with non-Legionella related CAP. Of 252 patients with Legionella, 131 had complete data and were included. Of non-Legionella patients, 160 were included as controls (Fig. 1) . Baseline characteristics are summarized in Table 1 . Patients with Legionella were often male, relatively younger, had less comorbidities (such as COPD and cancer), but were more frequently active smokers. Of cases, 126 (96%) were confirmed by UAT and 29 (22%) were confirmed by sputum PCR or culture. In the control group, the most frequently detected pathogens were Streptococcus pneumoniae (18.8%), Staphylococcus aureus (10.1%) and Haemophilus influenzae (8.1%). A further specification of pathogens in the control group is available in Table 2 .

In univariate regression of the original values (Table 3) , all six predictors were significantly associated with Legionella-related CAP. The strongest predictors were sodium, CRP and LDH levels (AUC respectively 0.76, 0.80 and 0.93). In multivariate regression, this association persisted for all parameters except for dry cough. The AUC of the multivariate model of these variables was 0.96 (95% CI 0.94-0.98).

In Table 4 As shown in Fig. 2 , a prediction score of 0 only occurred in non-Legionella-related CAP patients. Above, the number of cases gradually increased per score point. A prediction score of 5 or 6 points was only found in Legionella-related CAP patients (specificity 100%). The prediction score detected Legionella with a specificity of 93.1% and a sensitivity of 58.8% when a cut-off ≥ 4 was chosen. A cut-off ≥ 2 resulted in a sensitivity of 98.5% and a specificity of 50.6%. Figure 3 illustrates the receiver operating characteristics curve (ROC-curve) of the individual predictors and of the prediction score.

Legionella-related CAP is a disease with a high mortality rate and increasing incidence [1] [2] [3] [4] [5] [6] . It requires targeted antibiotic treatment, in an era where antibiotic resistance is rising and antibiotic stewardship is important. Although clinical symptoms of Legionella prove non-specific [15] , they can be a decisive factor in the treatment choice on admission [18] [19] [20] . This retrospective study further validated a prediction score based on six clinical parameters, that can be applied easily on admission, and found a high accuracy with an AUC of 0.89 (95% CI 0.86-0.93). We demonstrated that this score can potentially be used to rule-in or rule-out Legionella CAP, depending on the cut-off point chosen. Therefore, in patients presenting with mild to moderate disease symptoms, it could be applied both for early identification and specific treatment of those infected with Legionella, in particular in cases that are not detected by UAT. The negative predictive value of the score will likely be higher in an unselected population of hospital admitted CAP patients, since the incidence of Legionella is lower than in our population. All predictors were associated significantly with the outcome. However, temperature and platelets were no significant predictors in the multivariate analysis after dichotomization. Assumably, this can be explained by the wide range in which these variables occurred in both patients with Legionella CAP and with non-Legionella CAP.

Our study yielded an accuracy similar to that found by a Spanish study (AUC 0.86 (95% CI 0.81-0.90)), based on 82 cases [21] . It was higher than in a previous multinational validation study, which found an AUC of 0.73 (95% CI 0.65-0.81) [22] . This difference can be explained by a smaller sample size (37 cases). Baseline differences between the cases and controls (age, COPD and smoking) in our study were similar to both other studies [22] . This was not the case in a Japanese validation study published in 2017, in which participants were more often male and that also included patients with cancer [24] . They found a sensitivity of 94% and a specificity of 49% at a cut-off ≥ 2, resembling our present study.

In the literature two other diagnostic scoring systems for Legionella-related CAP were proposed, namely the Winthrop University score and the Community-Based Pneumonia Incidence Study Group scoring system. These two scoring systems were validated, but found unsuitable for diagnosing or excluding Legionella in a clinical setting, due to low accuracy [16, 17, 24, 25] .

A Japanese study group recently proposed a variation on the Legionella prediction score, which includes dyspnoea and gender instead of on temperature and platelets. This score performed well (AUC 0.93) in a Japanese validation cohort. However, in study populations outside Japan, male gender and dyspnoea were not identified as risk factors for Legionella-related CAP. Therefore this score may be less relevant [26] .

This multi-centre study included a large number of patients with Legionella-related CAP. The number of participants considerably exceeds the number that is due sufficient for validation of a prediction score with a dichotomous outcome, according to Toll et al. [23] . All hospital admitted patients with CAP were eligible for inclusion and data was collected from five different large hospitals with a wide geographical spread. This adds to the external validity of the study because it closely resembles a real-life clinical population. We chose to only include patients with complete data, so imputation of missing data could be avoided which adds on to the internal validity of the study. However, this has the potential to introduce some sort of selection bias but given the large sample of patients we believe the effect of this potential bias is likely small.

A weak point of this study is that its retrospective. Missing data on occurrence of especially (dry) cough lead to many exclusions. In a prospective study setting, this parameter would be easy to obtain. Furthermore, cases were retrospectively selected, based on positive microbiological tests. Mostly, this was the UAT, which does not detect species other than Legionella pneumophila serogroup 1. Because cultures and PCR have not been performed in all participants, some Legionella cases might have been missed. This could potentially influence the performance of the score. A Japanese study demonstrated a better performance of the Legionella prediction score for Legionella serogroup 1 (N = 11) than for other Legionella species (n = 23) [27] . This suggests that the score is particularly useful for detecting Legionella serogroup 1, which was detected in 96% of the cases in the present study.

Future research should validate the diagnostic scoring system prospectively, preferably in an unselected CAP population, in which Legionella is detected via UAT, PCR and cultures. This research could also analyse the accuracy of the scoring system, give more insight into performance of the score over the course of the disease, mild versus advanced disease, and investigate its clinical significance in addition to UAT. Moreover, longitudinal studies on clinical outcomes resulting from implementation of the test, such as change in antibiotic prescriptions, mortality, ICU admissions and of length of stay in the hospital, are needed.

This six-items prediction score detects Legionella related CAP infections with a high specificity of 93.1% (sensitivity 58.8%) in patients who score positive for at least four items. It is easy to implement in day to day practice with data readily available in every CAP patient and. Overall, based on our data and previous studies we believe it shows promise for further prospective validation and could contribute to targeted antibiotic treatment of Legionella-related CAP. 

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All authors contributed to data collection. RB and RD analysed the data and wrote the manuscript. WB supervised the project. All authors read and approved the final manuscript.

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The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

No permission by the medical ethical board was required for this retrospective study.

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