key: cord-0079070-st100bxe
authors: Zhang, Hao; Li, Zhen; Li, Chaoqun; Chen, Renfang; Liu, Tao; Jiang, Yiming
title: Antiviral Effect of Polyphenolic Substances in Geranium wilfordii Maxim against HSV-2 Infection Using in vitro and in silico Approaches
date: 2022-05-18
journal: Evid Based Complement Alternat Med
DOI: 10.1155/2022/7953728
sha: f54eeedd32306ca438cbad7e165686ad34900064
doc_id: 79070
cord_uid: st100bxe

BACKGROUND: Herpes simplex virus type 2 (HSV-2) infestation was the most widespread STD (sexually transmitted diseases) among humans and was the leading cause of infectious recurrent genital herpes. Existing therapies against HSV-2 did incompletely restrain the comeback of activated HSV-2 infestation. Geranium wilfordii Maxim had long been used as traditional Chinese medicine for treating the diseases owing to its anti-inflammatory and antiviral effects. Herein, the study was designed to investigate the antiviral activity of G.wilfordii and its potential effect in regulating the host's immune response. METHODS: To identify the stage of infection at which the compounds inhibited HSV-2, we performed virucidal, therapeutic, and prophylactic assays. The antiviral efficacy was evaluated by the analysis of viral components HSV-2 gD and VP16. The antiviral activities of these compounds were also evaluated by phenotypic analysis, such as cell proliferation and apoptosis. Molecular docking studies on candidate compounds were done to indicate binding interactions between the compounds and adopted compound targets. RESULTS: Quercetin, corilagin, and geraniin inhibited the replication of HSV-2, with geraniin showing greater TI. The obtained IC(50) value of quercetin was 204.7 μM and TI (IC(50)/EC(50)) was 5.1, whereas the obtained IC(50) value of corilagin was 118.0 μg/ml and TI was 4.05. Geraniin exhibited prominent antiviral activity with an IC(50) of 212.4 μM and an EC(50) of 18.37 μM, resulting in a therapeutic index (TI) of 11.56. Geraniin showed important in vitro virucidal activity through blocking viral attachment. Compared with the virus group, the apoptosis rates in quercetin-, corilagin-, and geraniin-treated groups were significantly decreased (p < 0.001).The expressions at the transcription genes of virus own replication key factors (including HSV-2 gD and VP16) and cytokines (including TBK1) of infected cells treated with quercetin, corilagin, and geraniin were inhibited. The in silico approaches demonstrated a high number of potential strong intermolecular interactions as hydrogen bonds between geraniin, corilagin, and the activity site of HSV-2 gD. Molecular docking studies demonstrated the effects of corilagin by targeting TBK1. CONCLUSIONS: Together, these results highlighted the importance of G.wilfordii treatment in HSV-2 infection and underscored its therapeutic potential. However, additional in vitro and in vivo research was required to validate our findings.

HSV-2 represented as a commonest contributor to genital ulcer illness globally, with epidemiological researches unanimously showing a tight association for HSV-2 and the threat of HIV infection and spreading [1] . HSV-2 was enveloped double-stranded DNA virus belonging to Herpesviridae [2] . HSV-2 was neurotropic pathogen that infected epithelial tissues and nerve termini, before retrograde spread within the peripheral nervous system, wherein viral latency was established [3] . Treatments currently directed against HSV infections were nucleoside analogs such as acyclovir, valacyclovir, penciclovir, and famciclovir that targeted viral DNA polymerase [4] . While current treatments inhibited active DNA replication during reactivation, there were currently no approved treatments targeting HSV-2 in their latent infection, reflecting the still-incomplete understanding of the mechanisms of latency [5] .

Natural products had been essential sources of new drugs for infectious diseases [6, 7] . It was reported that some natural compounds had shown some degree of anti-HSV-2 activity [8, 9] . As a traditional Chinese herbal medicine, G.wilfordii was frequently used for its antibacterial and antiviral properties [10] . G.wilfordii contained a variety of polyphenols [11] . Polyphenols exhibited a significant antimicrobial activity against a wide range of microbial infections [12] . Owing to their specific characters, polyphenolic substances were already put forward as wide-spectrum antiviral potential agents. Remarkably, geraniin and corilagin were the dominating active tannins of G.wilfordii, with the content of 14.34 and 12.32 mg/g, respectively [10] . Our previous studies used network pharmacology to identify anti-HSV-2 targets and pathways of certain bioactive components, and quercetin had the highest degree and betweenness centrality, thereby indicating that quercetin had the most important position in the network [13] . erefore in the current studies, we chose to carry out the design and experiments using geraniin, corilagin, and quercetin.

HaCaT cells were human keratinocytes that mimicked the cells HSV infects in vivo, and these cells could produce abundant quantities of HSV particles [14] . HaCaT keratinocytes also expressed cGAS, STING, TBK1, and IRF3 to similar extents [15] . e antiviral activities and toxicity of compounds, quercetin, corilagin, and geraniin, were assessed in vitro using HaCaT cells.

VP16, when combined to host proteins, was also a powerful transducible factor for five HSV instant early genes. HSV-2 VP16 protein was essential for lytic replication [16] . For all enveloped viruses, membrane fusion was a key early step for entering host cells and establishing infection. e binding of glycoprotein D with one of its receptors triggered the ability of gB to cause membrane fusion, and the gD determined the tropism of the HSV to the host cells [17] . RSAD2 (viperin) was an interferon-induced product associated with the restraint of reproduction of a striking array of RNA and DNA viruses. RSAD2 had been suggested to elicit these broad antiviral activities through interactions with a large number of functionally unrelated host and viral proteins [4] . In one previous experiment design, the co-localization of endogenous gD and RSAD2 was detected during HSV infection, but most of the endogenous RSAD2 mRNA would be degraded by UL41 when HSV infection [18] . In the cGAS-STING pathway, TBK1 and its upstream STING and downstream IRF3 constituted the core mechanism of IFNI production [19] , and HSV-2 interacted with this pathway through multiple mechanisms to produce immune escape [20] . TBK1 played a pivotal function in interferon generation and was an important constituent of antiviral immunity [21] . We measured the intracellular HSV-2 gD and HSV-2 VP16 gene expression levels to evaluate the effects of quercetin, corilagin, and geraniin on viral replication. Meanwhile, we assessed whether measuring compound-gene correlations would also be sufficient to elucidate compound targets. We tested whether the mRNA expression level of TBK1 and RSAD2 correlates with the potency of the compound.

Molecular docking of a compound molecule with its target molecule could provide vital information about the compound-receptor binding and affinity [22, 23] . Computer-aided compound design had become an important tool in discovering new molecules with particular pharmacological effects [24, 25] . Docosanol was a marketed HSV drug that inhibited viral fusion to the host cell. Docosanol was believed to prevent virus entry by interfering with the interaction between epithelial cell membrane receptors and HSV envelope proteins [26] . HSV-2 gD was an essential glycoprotein of HSV [27] . Furthermore, we used molecular docking to quantify the binding forces between three polyphenolic compounds and HSV-2 gD and compared it with that of docosanol and HSV-2 gD. Quercetin had interesting protective effects due to its large spectrum of biological activities. Studies had also shown that quercetin had antiviral effects on both RNA and DNA viruses [28] . To elucidate the mechanisms underlying the broad-spectrum antiviral activity of quercetin, we examined whether quercetin could interact with RSAD2 through molecular docking. BX795 was the first TBK1 inhibitor to be patented. One study demonstrated the antiviral activity of a kinase inhibitor, BX795, in inhibiting HSV infection [29] . is suggested that TBK1 and its functional interactors could be promising therapeutic targets towards HSV infection. To elucidate the mechanisms underlying the anti-HSV activity of quercetin and corilagin, we used molecular docking to quantify the binding forces between three polyphenolic compounds and TBK1 and compared it with that of MRT67307, a modified version of BX795 and TBK1.

us, the aim of the present study was to evaluate the antiherpes effects of quercetin, corilagin, and geraniin as well as investigate a potential mechanism of anti-HSV-2 action in vitro through a series of laboratory assays. Additionally, the candidate compounds were also assessed by molecular docking for determining the potential of physical interactions between the compounds and potential targets.

e virus strains used were aciclovir-sensitive HSV-2 G strains which were kindly donated by Prof Qinxue Hu, China Institute of Virology, Wuhan, China. HSV-2 were produced by propagating virus in Vero cells, and the titers of the virus based on TCID 50 were determined on HaCaT cells [14] . e cells were collected when 80% CPE (cytopathic effect) was observed, and HSV-2 was harvested by freezing at −80°C and thawing at 37°C and repeating it for 3 times [30] . Vero E6 cell line was donated by Qinxue Hu research group, and HaCaT cell line was obtained through Keygen Biotechnology Co., Ltd, Nanjing, Jiangsu, China. Cells were cultivated in DMEM complemented with 10% FBS and sustained in a 5% CO 2 humidified incubator at 37°C. 

Determination. Viral titers were determined by TCID 50 assay and MTT assay using HaCaT cells, which were loaded into 96-well tissue culture plates at a scale of 5,000 cells per well in DMEM and grown overnight in a monolayer to confluence. HaCaT cells were transfected with 10-fold serial dilutions of HSV-2 virus in a total final size of 100 μl and cultivated at 37°C for 2 hours. Cells were then cleaned with PBS to eliminate extracellular viruses and incubated in fresh medium for 72 hours. Cell survival rate was identified by MTT assay [14] .

Conditions. e same number of cells (0.5 × 10 4 ) of each group were seeded into each well of a 96-well tissue culture plate, and then seeded cells were cultivated overnight in an incubator at 37°C with 5% CO 2 . After a 2-h incubation with different concentrations (5x to 100x dilution) of virus stocks, plates were washed once with PBS. And then cells were incubated with fresh DMEM for 72 hours. Cell survival rate was determined by the MTT test. After incubating for 2 min to 120 min with 30x dilution of HSV-2 stocks (TCID 50 � 10 -1.5 ), cells were washed once with PBS to clear nonbinding virus. And again the cells were incubated with fresh DMEM for 72 hours, and, subsequently, cell survival rate was determined by the MTT test [32] .

e cytotoxicity of acyclovir, quercetin, corilagin, and geraniin were determined by MTT assay. HaCaTcells were seeded in 96-well plates and cultured in 10% DMEM for 24 h at 37°C in an atmosphere containing 5% CO 2 . e medium was then removed and working solution of the aforementioned compounds were severally added to individual HaCaT cells in plates with 6 wells in parallel for each dose and the plates were incubated for 72 h.

Cells treated without the experimental compounds were used as a control. ereafter, cell survival rate was determined by the MTT test as previously described. Subsequently, the half-maximal inhibitory concentration (IC 50 ) of experimental compound solution on HaCaT cells was automatically calculated using Bliss Principle according to the cell viability values obtained above [33] .

To evaluate the optimal antiviral concentration of the compounds for in vitro experiments, the cell survival rate was determined by MTT test. e different initial concentrations of compound dilutions were chosen according to previous literature and our preliminary experiment. Acyclovir, quercetin, corilagin, and geraniin were diluted with DMEM from 5000 ng/ml, 100 μM, 100 μg/ml, and 200 μM, respectively, at 2-fold multiplier for altogether 10 dilutions. Both untreated cell groups and virus-infected groups were set up. Virus infection conditions were performed as previously described. After virus infection of cells for 2 h, the cells were eluted with PBS and then each group was incubated using varied dilutions together by 72 hours followed by MTT experiments as described previously [34] . Viral inhibition rates were calculated according to the following formula: (OD experiment − OD virus)/(OD control − OD virus) ×100%. e concentration of each treatment, which reduced the virus replication rates by 50% (EC 50 ), was calculated using nonlinear regression in GraphPad Prism Software.

Modes. Virucidal assay: e direct viral inactivation was measured by MTT assay when compared to untreated controls. Mixtures of equal volumes of the ACV, quercetin, corilagin, or geraniin and 30-fold dilution of virus stock solution in serum-free DMEM were co-incubated for 120 min at 37°C. en, confluent monolayers of HaCaT cells received these treatments and were incubated for 120 min. e supernatants were subsequently removed, and infected cells were washed once with PBS and overlaid with serum-free DMEM, followed by incubation at 72 h. Acyclovir (156.25 ng/ml) was used as a positive control in all experiments. And then cell viability was determined by MTT assays. Viral inhibition rates were calculated as described above [35] . erapeutic assay: Confluent HaCaT cell monolayers were infected with 30-fold dilution of virus stock solution in serum-free DMEM for 120 min at 37°C. e viruses were removed after virus adsorption by washing with PBS, and cells were overlaid with DMEM containing different compound concentrations and acyclovir (156.25 ng/ml). e plates were processed after 72 h of incubation, as previously described for MTT assay. Viral inhibition rates were calculated as described above.

Prophylactic assay: Confluent HaCaT cell monolayers were pre-treated with different compound concentrations and acyclovir (156.25 ng/ml) at 37°C for 2 h before washing once with PBS. e medium was removed and 30-fold dilution of virus stock solution in serum-free DMEM was Evidence-Based Complementary and Alternative Medicine added to the cells for additional 2 h at 37°C. Unabsorbed viruses were removed by washing with PBS; cells were covered with DMEM and then processed after 72 h as previously described for the MTT assay. Viral inhibition rates were calculated as described above [36] .

HaCaT cells were plated at 2.5 × 10 4 cells per well in 24-well plates in DMEM overnight. HaCaT cells were then infected with HSV-2 virus as described above. Experimental design: HaCaT cells were divided into six groups; (i) the blank control group, (ii) virusinfected group without compound treatment, (iii) acyclovir (156.25 ng/ml)-treated group, (iv) quercetin (50 μM)-treated group, (v) corilagin (50 μg/ml)-treated group, and (vi) geraniin (100 μM)-treated group.

ree days after compound treatments, cell apoptosis was measured with the Annexin V apoptosis detection kit (Biyuntian Biotechnology Co. Ltd), and apoptosis data were analyzed using BD LSR II Flow Cytometer and FlowJo software [37] .

PCR primers were purchased from Invitrogen. Total RNA was extracted using TRIzol reagent (Sangon Biotech, B511311). All TaqMan expression reagents were purchased from Jiangsu realgen-bio Co. e quantitative PCR (qPCR) experiments were performed as per the manufacturer's instructions with TaKaRa RT-PCR Kit (Takara, RR064 A) [38] . All PCR primers are shown in Table 1 . [39] . e interactions were visualized using Discovery Studio Visualizer [40] . e binding efficiency of each target to the original ligand and prototype compounds was measured using LibDock score [41] . e LibDock scores were predicted values of the free energy of protein-ligand binding, and a higher absolute value represents a higher affinity. e most reliable docking pose of each molecule was accepted on the basis of the highest LibDock score and further appraised using Discovery studio visualizer to examine the molecular interactions.

Nonlinear regression of concentration-response curves was used with GraphPad Prism 8 for determination of the IC 50 and EC 50 values. Statistical analyses were determined by one-way ANOVA and were considered significant when p < 0.05.

Based on the results obtained from TCMSP database and PubChem database, the composition of G.wilfordii are presented in Table 2 , while 3D structures of quercetin, corilagin, and geraniin are presented in Figure 1 . Quercetin and geraniin were both relatively well absorbed in the intestine, while corilagin had poor oral bioavailability. However, the clinical application of geraniin was limited, due to its poor drug-like physicochemical properties, whereas corilagin exhibited better drug-like properties.

e TCID 50 value of HSV-2 stocks was 10 -1.5 (Figure 2(a) ). Our results indicated that significant difference in viability was observed (p < 0.0001) at 5x to 30x dilution of virus stock group with less than 60% cell viability (Figure 2 (b)). We observed (p < 0.0001) a sharp downward cell viability under the condition of virus adsorption for 50 min to 120 min with less than 60% cell viability (Figure 2(c) ). In this assay, HaCaT cells were incubated with 30x dilution of virus stocks for 2 h. ese conditions were employed in all experiments described below, except where specifically noted.

e maximum nontoxic concentrations in HaCaT cells were defined by MTT assay. Using HaCaT cell as a prototypical cell line, strong discrimination of cytotoxicity (cell viability � 53.9%, p < 0.0001) was clearly apparent at 5000 ng/ml of acyclovir (Figure 3(a) ). Quercetin showed significant cytotoxicity (cell viability＜60%, p < 0.0001) at concentrations of ≥15.625 μM (Figure 3(b) ). Corilagin exhibited significant cytotoxicity (cell viability＜60%) at concentrations of ≥50 μg/ml (Figure 3(c) ). Geraniin exhibited no obvious cytotoxicity (cell viability＞70%) at experimental concentrations ( Figure 3(d) ). (Figure 4(a) ), 50 μM of quercetin (Figure 4(b) ), 50 μg/ml of corilagin (Figure 4(c) ), and 100 μM of geraniin (Figure 4(d) ) were the ones exhibiting the strongest cytoprotective effect (p < 0.0001). Hence, these concentrations were selected to perform the following experiments. e inhibitory activities of acyclovir, quercetin, corilagin, and geraniin are summarized in Figure 5 along with therapeutic index (TI) values given as the ratio IC 50 /EC 50 . Quercetin, corilagin, and geraniin inhibited the replication of HSV-2, with geraniin showing greater TI. e obtained IC 50 value of acyclovir was 5449 ng/ml ( Figure 5(a) ) and a therapeutic index (IC 50 /EC 50 ) was 224.1 ( Figure 5(e) ). e obtained IC 50 value of quercetin was 204.7 μM ( Figure 5(b) ) and a therapeutic index (IC 50 /EC 50 ) was 5.1 ( Figure 5 (f ))), whereas the obtained IC 50 value of corilagin was 118.0 μg/ml ( Figure 5 (c))) and a therapeutic index (IC 50 / EC 50 ) was 4.05 ( Figure 5(g) ). In addition, geraniin exhibited prominent antiviral activity with an IC 50 of 212.4 μM ( Figure 5(d) ) and an EC 50 of 18.37 μM, resulting in a therapeutic index (TI) of 11.56 ( Figure 5(h) ).

Compound Presence. All concentrations of quercetin, corilagin, and geraniin reduced HSV-2 replication in a dosedependent manner. In therapeutic assay, acyclovir showed significant inhibition of HSV-2 with an 82.32% viral suppression rate. High-dose quercetin exhibited similar viral inhibition rates (77.286%) comparable to acyclovir (Figure 6(a) ). Corilagin and geraniin showed an inferior inhibition rate at high dose (50.58 and 48.3% compared to the control, respectively). In virucidal assay, the viral inhibition rate of geraniin was 187.2% (Figure 6(b) ), which proved superior to the 67.99% inhibition rate seen for acyclovir. Corilagin and quercetin showed an inferior inhibition rate at high dose (121.2 and 147.3% compared to the control, respectively). In prophylactic assay, the inhibition rate of acyclovir was markedly decreased (5.49% compared to the control). Corilagin and quercetin showed notable inhibition rates at high dose (46.03 and 45.1% compared to the control, respectively) ( Figure 6(c) ).

To examine the compound's antiviral mechanism of action, we examined apoptosis in vitro through Annexin V/propidium iodide flow cytometry. As depicted in Figure 7 (a), after 72 -h treatment, HSV-2-infected HaCaT cells underwent obviously late apoptosis and cell death rate (11.5% and 12.5%, respectively) during lytic infection compared to control group (1.12% and 1.45%, respectively). Compared with the virus group, the apoptosis rates in the other four compoundtreated groups were significantly decreased (p < 0.001) (Figure 7(b) )), suggesting the inhibition of cell apoptosis by acyclovir, quercetin, corilagin, and geraniin treatment. We found that HaCaT cells in quercetin-treated conditions exhibited apoptosis rates comparable to that of acyclovir (5.29% and 5.22%, respectively).

To determine if quercetin, corilagin, and geraniin specifically impacted virus replication, we checked viral gD and VP16 levels by QPCR. Quercetin, corilagin, and geraniin showed the greatest inhibition in therapeutic assay, being less potent in virucidal assay, and least potent in prophylactic assay (Figures 8(a) Evidence-Based Complementary and Alternative Medicine effect in virucidal assay was commensurable to that of geraniin in therapeutic assay (Figure 8(i), 8(j) ). We inferred from this that geraniin might have the potential to function as antiviral drugs by directly interacting with critical proteins of the virus. e ribonuclease UL41 of HSV could degrade the mRNA of RSAD2 to promote HSV replication [43] . Vaginal tissue from mice infected locally with HSV-2 showed strong RSAD2 expression in cells located to the area of infection [44] . e action of geraniin in prophylactic assay promoted TBK1 was an important regulator of innate immune responses. Optimized TBK1 action was indispensable for virus clearance, and overexcited TBK1 activation might accelerate inflammatory damage during viral infection [21] . BX795, an antagonist for TBK1, vigorously restrained HSV-1 infection by inhibiting viral protein synthesis. Quercetin and corilagin showed inhibitory effects on TBK1 indicating that they might inhibit HSV-2 replication by targeting TBK1 (Figure 8(d), 8(h)) ).

We speculated that quercetin, corilagin, and geraniin activity could fulfill a specific regulatory function by acting on particular targets including viral components and immune response-related factors. As a preliminary test for this hypothesis, we conducted molecular docking of quercetin, corilagin, and geraniin targeting HSV-2 gD, RSAD2, and TBK1 proteins (PDB ID : 4MYV, 6B4C, and 4IM0, respectively). e interaction energies of the quercetin, corilagin, and geraniin with HSV-2 gD, RSAD2, and TBK1 proteins are shown in Table 3 . Docosanol could repress HSV-2 entry by disrupting the mutual association for epithelial cell layer receptors and HSV-2 envelope proteins. BX795 could inhibit the phosphorylation of TBK1 and block HSV infection. MRT67307, a modified version of BX795, was a potent TBK1 inhibitor. e compounds docosanol (LibDock score � 101.728) (Figure 9 (g) and 9(h)) and MRT67307 (LibDock score � 119.478) (Figure 10 (g) and 10(h))were selected on the basis of the best interaction energies for further analysis. e binding modes of HSV-2 gD with four selected compounds are displayed in Figure 9 . Geraniin demonstrated high occupation in the active site of HSV-2 gD (Figure 9 (e), 9(f )). In addition, the conformation of quercetin, corilagin, and geraniin reviewed by the docking simulations occupied the same pocket with numerous hydrogen bonds for HSV-2 gD (Figure 9 (a), 9(c), 9(e)). Geraniin interacted with HSV-2 gD at site 3 by twelve Van der Waals, one carbon-hydrogen, and three hydrogen bonds (Figure 9(f ) ). Corilagin interacted with HSV-2 gD at site 3 by eleven Van der Waals, two carbonhydrogen, and four hydrogen bonds (Figure 9(d)) ). It also formed two hydrogen bonds and six Van der Waals interactions with TBK1 at site 2 ( Figure 10(e) , 10(f )). Quercetin interacted with HSV-2 gD at site 3 by eight Van der Waals, one carbon-hydrogen, and two hydrogen bonds (Figure 9(b) ). It also formed two hydrogen bonds, four Van der Waals interactions, and one carbon-hydrogen with RSAD2 at site 4 ( Figure 11(a), 11(b) ). Figure 9 showed that docosanol was docked into active sites of HSV-2 gD domain at ARG130 (Figure 9 (h)), and LibDock score was 101.728. Both corilagin and geraniin could bind to HSV-2 gD against the residues ARG82 and GLU146 and form hydrogen bonds (Figure 9 (d), 9(f )) with higher LibDock score of 139.12 and 133.906 compared with docosanol. e docking results demonstrated that quercetin was docked into active sites of RSAD2 domain at LYS80, LYS114, HIS115, GLU131, and THR133 (Figure 11(b) ) and showed good LibDock interaction energy (LibDock score � 94.3065) higher than ACV (LibDock score � 86.3175).

Structure-based molecular docking simulations in Figure 10 indicated that corilagin could bind to the active site GLU178, ARG228, and LYS567 of TBK1 by forming hydrogen bonds (Figure 10 

Vero cells were usually used to propagate and grow large batches of HSV-2 for research and especially virus batches grown for use in vaccine challenge studies [45] . Although Vero cells was a model cell line for pathogenic HSV-2, they likely did not fully recapitulate all aspects of infection in primary cells, such as human genital epithelial cells, nor does this system fully recapitulate the complex cellular milieu in a human patient [46] . HaCaT culture could artificially mimic the HSV-2 infection within the real reproductive tract microenvironment [47] . In the present study, we used HaCaT cells as an in vitro assay platform, which was a long-lived, spontaneously immortalized human keratinocyte line with potentially different signalling pathways compared to non-immortalized cells [15] . Our results showed that Hacat cells could be infected with HSV-2, and the infection efficiency on the HaCaT cells estimated that HaCaT cells could be used as a susceptible cell line for HSV-2 infection. HSV-2 infection represented a serious public threat and the unavailability of potential antiviral drugs emphasized the need for identification of new leads [48] . Multiple bioassays were used to identify quercetin, corilagin, and geraniin obtained from G.wilfordii with promising anti-HSV-2 effects. In the three ways of therapeutic, virucidal, and prophylactic modes, quercetin, corilagin, and geraniin could exert an individual antiviral activity on the infected HSV-2 cells. By confirming the effectiveness of geraniin, one promising compound, as a virus-host cell fusion inhibitor, HSV-2 entry host cells can be prevented to treat HSV-2 infection. In addition, geraniin had lower cytotoxicity against HaCaT cells and a better therapeutic index against HSV-2.

ese findings strongly suggested that geraniin might be a promising candidate compound for the effective treatment of HSV-2 infection. e function of the HSV tegument protein VP16 played a crucial role in the HSV life cycle, and it was a powerful transcriptional activator that specifically acted on IE (Immediate Early) genes [49] .

us, the analysis of the interaction between VP16 and compounds would be meaningful for the further development of antiviral compounds. HSV-2 was an enveloped DNA virus. For all enveloped viruses, membrane fusion was a key early step for entering host cells and establishing infection. e binding of glycoprotein D with one of its receptors triggered the ability of gB to cause membrane fusion, and the gD determined the tropism of the HSV to the host cells. e inhibition of the membrane fusion process was a promising strategy for combating infection [50] . It was found by QPCR assay that quercetin, corilagin, and geraniin could attenuate the expression of HSV-2 gD and VP16 at the transcription gene level. According to the above comprehensive data, the antiviral activity of quercetin, corilagin, and geraniin on infected cells was closely related to the replication process of HSV-2. HSV-encoded molecules also interfered with cellular apoptosis [51] , which was an important innate immune mechanism for eliminating pathogen-infected cells. HSV initiated apoptosis through a parallel pathway involving cGAS and STING [52] . HSV immediate-early protein ICP0 triggered apoptosis during HSV infection to influence viral pathogenesis [53] . However, the exact mechanisms underlying the promotive effect of apoptosis on such HSV infection-associated diseases remained unknown. e detection of apoptosis had provided the tools for drug discovery as well as the experimental means for validation of drug action in clinical specimens [54] . Our in vitro study indicated that the rate of apoptosis was obviously higher in virally infected group compared with quercetin, corilagin, and geraniin treatment group. After treatment of HaCaT cells with quercetin, corilagin, and geraniin, the apoptosis caused by HSV-2 was inhibited, indicating that above compounds could inhibit the apoptosis of HSV-2-infected cells and this might be an important mechanism by which above compounds exerted anti-HSV-2 effects.

Molecular docking approaches had rendered perceptions into the ligand-receptor interaction manner and in the discovery of potential HSV-2 inhibitors. e availability of an inhibitor bound protein structure rendered an outstanding opportunity to provide the data linked to their interaction [55] . It would be beneficial to consolidate the derived data in structure-based drug design [56] . TBK1 and RSAD2 protein played an essential role in innate immunity against HSV-2 infection, meanwhile HSV-2 gD had prime function in the entry of virus into the host, and thus they were chosen as potential targets for antiviral search. Quercetin, corilagin, and geraniin from G.wilfordii were docked on the predicted binding site. Corilagin and geraniin presented the most promising binding energies targeting HSV-2 gD. We also compared these results with the binding potential of docosanol.

e comparative analysis suggested that corilagin and geraniin showed more promising potential than anti-HSV-2 compounds docosanol. From molecular docking results based on the cumulative effect of estimated affinity and ligand efficiency, we selected corilagin, which gave similar results to the TBK1 inhibitor MRT67307 and could be considered to act as specific antagonists. Additionally, these in silico and in vitro findings required further experimental validation to advance in the drug discovery pipeline [57] .

ere was a growing interest in naturally derived products providing outstanding health merits with relatively safe profiles, useful for the treatment of HSV-2 infections. In this article, the antiviral activity and mechanism of quercetin, corilagin, and geraniin against HSV-2 were investigated in vitro. Geraniin exhibited prominent antiviral activity with an IC 50 of 212.4 μM and an EC 50 of 18.37 μM, resulting in a therapeutic index (TI) of 11.56. Both quercetin and corilagin solution could provide antiviral effects for therapeutic, virucidal, and prophylactic inactivation of HSV-2 infection, and geraniin showed important in vitro virucidal activity, and this indicated that geraniin might exert a good anti-HSV-2 activity in vitro through inhibiting membrane fusion of HSV-2 targeting HSV-2 gD. At the level of virus replication, quercetin, corilagin, and geraniin could significantly inhibit the transcriptional gene expressions levels of HSV-2 VP16 and gD. Apoptosis assay showed that the apoptosis rates of HaCaT cells infected by compound-treated HSV-2 were decreased markedly.

HSV-2 gD was a promising target for developing novel HSV-2 inhibitors. In this analysis, we selected a set of three compounds from G.wilfordii to examine their interaction posed inside the active site of the HSV-2 gD complex. Our findings revealed the efficacy of corilagin and geraniin against HSV-2 gD complex. In selected corilagin and geraniin, LYS122, ARG82, and GLU146 had the highest contribution.

Meanwhile, we studied the antagonistic behavior of the previously known molecule MRT67307 in comparison to quercetin and corilagin, which have natural origin towards its binding site in the TBK1. Quercetin and corilagin molecules successfully interacted with binding site of TBK1 in the receptor. It depicted a fairly similar binding affinity for the corilagin towards the receptor as compared to MRT67307.

As docking experiments only provided stable static binding modes for a ligand inside the active site of a protein [58] , further research focused on the authentication of its activity along with pharmacokinetic and pharmacodynamic properties in vivo and in clinical trials is required [22] .

All data are available from the corresponding author upon reasonable request.

e authors report that there are no conflicts of interest in this work.

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Design, synthesis and biological activity evaluation of S-substituted 1H-5-Mercapto-1,2,4-Triazole derivatives as antiproliferative agents in colorectal cancer

Discovery and in silico evaluation of aminoarylbenzosuberene molecules as novel checkpoint kinase 1 inhibitor determinants

Approved antiviral drugs over the past 50 years

e structural basis of herpesvirus entry

Immune-boosting, antioxidant and anti-inflammatory food supplements targeting pathogenesis of COVID-19

In vitro and in vivo activity, tolerability, and mechanism of action of BX795 as an antiviral against herpes simplex virus 2 genital infection

Human herpesvirus type 1 and type 2 disrupt mitochondrial dynamics in human keratinocytes

DNA repair of myeloma plasma cells correlates with clinical outcome: the effect of the nonhomologous end-joining inhibitor SCR7

Antiviral effect of Chinese herbal prescription JieZe-1 on adhesion and penetration of VK2/E6E7 with herpes simplex viruses type 2

Recycling drug screen repurposes hydroxyurea as a sensitizer of glioblastomas to temozolomide targeting de novo DNA synthesis, irrespective of molecular subtype

e Chinese herbal prescription JZ-1 induces autophagy to protect against herpes simplex Virus-2 in human vaginal epithelial cells by inhibiting the PI3K/Akt/mTOR pathway

Antiviral potential of spondias mombin L. Leaves extract against herpes simplex virus type-1 replication using in vitro and in silico 14 Evidence-Based Complementary and Alternative Medicine approaches

Antiviral mechanism of carvacrol on HSV-2 infectivity through inhibition of RIP3-mediated programmed cell necrosis pathway and ubiquitin-proteasome system in BSC-1 cells

6-Phosphofructo-2-kinase/ fructose-2,6-Biphosphatase-2 regulates TP53-dependent paclitaxel sensitivity in ovarian and breast cancers

Cardiac specific PRMT1 ablation causes heart failure through CaMKII dysregulation

Integrated phytochemical analysis based on UPLC-Q-TOF-MS/MS, network pharmacology, and experiment verification to explore the potential mechanism of platycodon grandiflorum for chronic bronchitis

Structure-based drug designing and immunoinformatics approach for SARS-CoV-2

Study on the interactions between caffeoylquinic acids with bovine serum albumin: spectroscopy, antioxidant activity, LC-MSn, and molecular docking approach

Applications of machine learning in drug discovery and development

e interaction mechanism between herpes simplex virus 1 glycoprotein D and host antiviral protein viperin

STING agonists enable antiviral cross-talk between human cells and confer protection against genital herpes in mice

Griffithsin carrageenan fast dissolving inserts prevent SHIV HSV-2 and HPV infections in vivo

Monoclonal antibodies, derived from humans vaccinated with the RV144 HIV vaccine containing the HVEM binding domain of herpes simplex virus (HSV) glycoprotein D, neutralize HSV infection, mediate antibody-dependent cellular cytotoxicity, and protect mice from ocular challenge with HSV-1

Herpesvirus gBinduced fusion between the virion envelope and outer nuclear membrane during virus egress is regulated by the viral US3 kinase

Natural products-derived chemicals: breaking barriers to novel anti-hsv drug development

Pathogenesis and virulence of herpes simplex virus

Receptors and ligands for herpes simplex viruses: novel insights for drug targeting

Reducing viral inhibition of host cellular apoptosis strengthens the immunogenicity and protective efficacy of an attenuated HSV-1 strain

Apoptosis and necroptosis as host defense strategies to prevent viral infection

e major tegument protein of bovine herpesvirus 1, VP8, interacts with DNA damage response proteins and induces apoptosis

Targeting apoptosis in cancer therapy

Identification of potential plant bioactive as SARS-CoV-2 Spike protein and human ACE2 fusion inhibitors

Targeting the protein-protein interface pocket of Aurora-A-TPX2 complex: rational drug design and validation

Plantbased analogues identified as potential inhibitor against tobacco mosaic virus: a biosimulation approach

A new insight into proteinprotein interactions and the effect of conformational alterations in PCNA

Hao Zhang conceived, designed, and planned the study. Hao Zhang and Chaoqun Li acquired and analyzed the data. Renfang Chen and Zhen Li interpreted the results. Hao Zhang drafted the manuscript. Tao Liu and Yiming Jiang contributed to the critical revision of the manuscript. All authors read and approved the final manuscript.