key: cord-0252780-oez3l9kn authors: Wapling, Johanna; Srivastava, Seema; Shehu-Xhilaga, Miranda; Tachedjian, Gilda title: Targeting Human Immunodeficiency Virus Type 1 Assembly, Maturation and Budding date: 2007-07-20 journal: Drug Target Insights DOI: nan sha: f3042820ac417ec01ca0e15a4232e24b8d78ba74 doc_id: 252780 cord_uid: oez3l9kn The targets for licensed drugs used for the treatment of human immunodeficiency virus type 1 (HIV-1) are confined to the viral reverse transcriptase (RT), protease (PR), and the gp41 transmembrane protein (TM). While currently approved drugs are effective in controlling HIV-1 infections, new drug targets and agents are needed due to the eventual emergence of drug resistant strains and drug toxicity. Our increased understanding of the virus life-cycle and how the virus interacts with the host cell has unveiled novel mechanisms for blocking HIV-1 replication. This review focuses on inhibitors that target the late stages of virus replication including the synthesis and trafficking of the viral polyproteins, viral assembly, maturation and budding. Novel approaches to blocking the oligomerization of viral enzymes and the interactions between viral proteins and host cell factors, including their feasibility as drug targets, are discussed. HIV-1 is a major public health problem affecting an estimated 40 million individuals worldwide (www. unaids.org). Although it has been over 20 years since HIV-1 was identifi ed as the etiologic cause of acquired immune defi ciency syndrome (AIDS) an effective vaccine is not available. Thus, apart from public health measures that aim at HIV-1 prevention, the only effective strategy for controlling HIV-1 infections and lowering HIV-1 transmission is the use of antiretroviral drugs either for the treatment or prevention of infections. Current antiretroviral drugs belong to four classes, the nucleoside/nucleotide reverse transcriptase inhibitors (NRTIs), nonnucleoside reverse transcriptase inhibitors (NNRTIs), protease (PR) inhibitors (PI) and fusion inhibitors (Vivet-Boudou et al. 2006; De Clercq, 1998; Abdel-Rahman et al. 2002; Manfredi and Sabbatani, 2006) . NRTIs and NNRTIs are respectively, competitive and allosteric inhibitors of the HIV-1 reverse transcriptase (RT) and act early in the viral life-cycle by blocking the conversion of the viral RNA genome into a double stranded proviral DNA precursor (Shehu-Xhilaga et al. 2005) . Fuzeon (enfuvirtide or T20) is a peptide that also acts early in the virus life-cycle by preventing viral entry through interaction with the gp41 transmembrane protein (Shehu-Xhilaga et al. 2005) . In contrast, PIs inhibit the late stage of virus replication by blocking the specifi c cleavage of Gag and Gag-Pol polyproteins to mature structural proteins and enzymes (Shehu-Xhilaga et al. 2005) . Early antiretroviral regimens consisted of one or two RTIs, which were delivered as sequential monotherapy and led to treatment failure (Piacenti, 2006) . The advent of combination therapy, or highly active antiretroviral therapy (HAART) since 1996 has been responsible for a dramatic decrease in AIDS mortality (Palella et al. 1998) . Current HAART regimens generally comprise three antiretroviral drugs, usually two NRTIs and either a PI or an NNRTI (Yeni et al. 2002) . While an armoury of agents is available for the treatment of HIV-1 patients, new drugs and drug targets need to be identifi ed due to drug toxicity and the eventual emergence of drug resistant strains to current antiretroviral inhibitors (Clavel and Hance, 2004) . Moreover, resistance to one drug normally results in cross-resistance to inhibitors of the same class, rendering a large number of agents to limited clinical use (Clavel and Hance, 2004) . Therefore, the development or availability of new drugs such as Fuzeon, the HIV-1 integrase inhibitor raltegravir (MK-0518) (Grinsztejn et al. 2007 ) and the CCR5 antagonist maraviroc (Stephenson, 2007) that remain active against drug resistant virus is essential for the continuing success of HAART (Yeni, 2006) . The increased understanding of how HIV-1 reproduces and interacts with the host cell machinery has resulted in the identifi cation of potential drug targets, which can be exploited for the development of new classes of inhibitors. Here we describe strategies and agents that block the late stages of HIV-1 replication including the synthesis and traffi cking of viral polyproteins, viral assembly, maturation and budding. Novel approaches to blocking the oligomerization of viral enzymes and the interactions between viral proteins and host cell factors are discussed including their feasibility as drug targets. While peptidomimetic PIs act at the late stage of HIV-1 replication to block viral maturation, this review will deal with agents that inhibit HIV-1 PR by novel mechanisms that are distinct to these transition state mimetics that are competitive inhibitors of the HIV-1 PR. Following virus attachment, fusion and uncoating the single stranded positive sense RNA genome of HIV-1 is reverse transcribed by the viral RT into a proviral DNA precursor in a reverse transcription complex (RTC) containing viral and possibly host cell factors (Fig. 1) . The RTC matures into a preintegration complex (PIC) and traffi cs to the nucleus where the viral cDNA is inserted into the host cell chromosome by the HIV-1 integrase (IN) (Telesnitsky A. and Goff, 1997) . The processes from entry up to and including integration are defi ned as the early steps in the viral life cycle. The late stage of virus replication begins with transcription of the viral mRNAs from the integrated provirus (Fig.1) . Singly and multiply spliced mRNAs encode the HIV-1 envelope proteins and regulatory/accessory proteins, respectively (Rabson and Graves, 1997) . Pr55 gag (Gag) and Pr160 gag-pol (Gag-Pol) polyproteins are translated from unspliced mRNAs (Swanstrom, 1997) . Formation of two types of polyproteins from the same unspliced mRNA is mediated by a ribosomal frameshifting mechanism that brings the pol sequence in the same reading frame as gag. Perturbation of ribosomal frameshifting leads to changes in the Gag and Gag-Pol ratio that is detrimental to virus assembly, morphogenesis and release (Swanstrom, 1997) . Gag encodes the viral structural proteins matrix (MA), capsid (CA), nucleocapsid (NC), p6 and two spacer peptides, p1 and p2. Gag-Pol also encodes MA, CA and NC in addition to the three viral enzymes, PR, RT and IN. After translation, Gag and Gag-Pol are targeted to the host cell plasma membrane, a process that is dependent on the myristoylation of the N-terminus of Gag ( Fig. 1) (Swanstrom, 1997) . Inhibition of myristoylation disrupts the proper targeting of Gag and Gag-Pol to the plasma membrane (Swanstrom, 1997) . Gag-Gag, Gag/Gag-Pol and Gag-RNA interactions are also essential for the proper assembly and maturation of infectious virions. Gag and Gag-Pol assemble at the plasma membrane along with viral envelope glycoproteins gp120 and gp41 to form immature viral particles (Fig. 1) . Gag is necessary and suffi cient for virus particle formation (Freed, 1998; Swanstrom, 1997) . The viral genomic RNA is also packaged into virions through interactions with the NC of Gag and a psi packaging signal in the genome (Swanstrom, 1997) . As the newly assembled virions bud from the cell it is believed that Gag-Pol polyproteins oligomerize in order to activate the HIV-1 PR by forming an active PR homodimer. This results in the sequential cleavage of Gag and Gag-Pol into the mature structural proteins and enzymes Pettit et al. 1998) . Agents that bind to domains in Gag or Gag-Pol and modulate their oligomerization are likely to have a negative effect on virus assembly, maturation and budding (Fig. 1) . Agents that interfere with HIV-1 PR mediated cleavage of Gag and Gag-Pol result in the production of immature viral particles that are non-infectious (Kohl et al. 1988) . Virus particle budding and egress is mediated by interactions of viral proteins such as the p6 late domain with components of the endosomal sorting machinery. Ion channels formed by viral protein U (Vpu) also facilitate viral particle egress from the host cell. Below we describe in more detail the HIV-1 Gag and Gag-Pol polyproteins are encoded by overlapping open reading frames on the same unspliced mRNA. During translation Gag-Pol is synthesized by a -1 ribosomal frameshifting mechanism that occurs at a frequency of 5 to 10% of Gag translation events (Jacks et al. 1988b) . Similar frameshifting mechanisms are also used by other retroviruses including Rous sarcoma virus and Mouse mammary tumor virus in order to regulate expression of Gag-Pol (Jacks and Varmus, 1985; Jacks et al. 1987; Jacks et al. 1988a ). The HIV-1 frameshift site is a heptanucleotide AU-rich sequence (UUUUUUA) found at the 3′ end of the NC coding sequence and is conserved amongst HIV-1 isolates. This slippery sequence and a downstream RNA stem loop structure stall the ribosome during the synthesis of Gag, allowing the ribosome to slip Figure 1 . Overview of the HIV-1 life-cycle. Early events in virus replication include attachment, fusion and uncoating of the virus followed by reverse transcription in the cytoplasm of the cell, nuclear import of the preintegration complex and integration of the proviral DNA precursor into the host cell chromosome. Late events begin with transcription of unspliced and spliced RNA from the provirus and export of the mRNAs to the cytoplasm, resulting in the translation of Gag, Gag-Pol, Env and the accessory and regulatory proteins of HIV-1. Regulation of Gag-Pol synthesis is mediated by a ribosomal frameshifting mechanism from unspliced mRNA that also expresses Gag. Myristoylation of Gag is necessary for traffi cking of Gag and Gag-Pol to the site of viral assembly. Assembly is driven by interactions between Gag-Gag, Gag/Gag-Pol, Gag-RNA. Viral budding and egress involves host cell factors. During or shortly after budding the HIV-1 PR cleaves the Gag and Gag-Pol polyproteins resulting in a mature and infectious viral particle. back one nucleotide and enable synthesis of the Gag-Pol fusion protein (Jacks et al. 1988b ). This sequence, the stem-loop structure and its stability and adjacent interacting sequences are believed to be the key components of the frameshifting signal (Jacks et al. 1988b; Kollmus et al. 1994; Hill et al. 2005) . Details of a recently reported NMR structure and an analysis of current HIV-1 frameshifting models have recently been reviewed (Brierley and Dos Ramos, 2006) . Studies demonstrate that perturbation of the Gag/Gag-Pol ratio result in major defects in virus replication, suggesting that interfering with ribosomal frameshifting represents a viable drug target. Alteration of the Gag/Gag-Pol ratio, by engineering vectors with gag and pol genes in the same open reading frame, results in major defects in assembly and budding (Karacostas et al. 1993; Park and Morrow, 1991) . The block in virus assembly is partially overcome by inhibition of the HIV-1 PR, suggesting that increased HIV-1 PR activity is responsible for the defect (Karacostas et al. 1993) . A later study, in which the impact of decreasing the ratio of Gag/Gag-Pol on virion production was determined by co-transfection of plasmids expressing Gag and Gag-Pol alone demonstrate that the maintenance of this ratio is not only important for HIV-1 replication but also for virion RNA dimer formation and stability (Shehu-Xhilaga et al. 2001a ). Furthermore, a decrease in Gag-Pol translation results in major defects in virus maturation and HIV-1 infectivity (Dulude et al. 2006 ). The small molecule, 1,4bis- [N-(3-N,N-dimethylpropyl) amidino]benzene tetrahydrochloride (RG501 , Table 1 ), is thought to enhance ribosomal frameshifting of HIV-1 by binding to the RNA stem loop structure of the ribosomal frameshifting signal resulting in increased ribosomal pausing (Hung et al. 1998) . The imbalance in the resulting Gag/Gag-Pol ratio is associated with inhibition of acute and chronic HIV-1 infection in CCRF-CEM cells and peripheral blood mononuclear cells. During the late phase of the viral life cycle, Gag polyproteins are targeted to the plasma membrane, where they are believed to colocalise to lipid raft microdomains for assembly into immature virions (Morikawa et al. 1996; Bryant and Ratner, 1990; Bouamr et al. 2003; Ding et al. 2003; Holm et al. 2003; Tang et al. 2004) . Membrane targeting of Gag is mediated by the N-terminal myristoyl group in concert with conserved basic amino acids at the N-terminus of the MA domain of Gag (Bryant and Ratner, 1990; Facke et al. 1993; Ono and Freed, 1999; Ono et al. 2000) . Myristic acid is a saturated 14-carbon fatty acid, post transationally attached to the N-terminal glycine of both Gag and Gag-Pol (Veronese et al. 1988 ). Myristoylation of Gag but not Gag-Pol is critical for targeting these polyproteins to the plasma membrane (Park and Morrow, 1992; Smith et al. 1993) . Mutations that interfere with Gag myristoylation inhibit viral budding and misdirect virion assembly to the cytosolic fraction (Gottlinger et al. 1989; Bryant and Ratner, 1990) . However, complete inhibition of Gag myristoylation is necessary to block HIV-1 budding (Morikawa et al. 1996) . Myristoylation is a two-step process involving activation of myristate to myristoyl-CoA by acyl-CoA synthetase and transfer of the myristoyl moiety from the myristoyl-CoA substrate to the N-terminal glycine of Gag by the enzyme N-myristoyltransferase (NMT) (Morikawa et al. 1996; Veronese et al. 1988 ). This pathway has been utilized to deliver alternate myristoylation substrates that perturb viral assembly. The myristic acid analogue 12-azidododecanoic acid is a potent inhibitor of HIV-1 production in acute and chronically infected T-cell lines, exhibiting a maximum inhibitory effect between 10-50 µM at noncytotoxic concentrations, however the mechanism of action is not defi ned . Another analogue, 4-oxatetra-decanoic acid, reduces HIV-1 replication in a T-cell line at 18 µM (Langner et al. 1992) . Heteroatom-substituted analogs of myristic acid such as 12-methoxydodecanoate (13-oxamyristate or 13-OxaMyr), 5-octyloxypentanoate (6-oxamyristate or 6-OxaMyr), 11-ethylthioundecanoic acid and 12-thioethyldodecanoic acid act as alternate substrates for Gag myristoylation (Bryant et al. 1989; Bryant et al. 1991; Parang et al. 1997 ) and can prevent membrane binding of the modifi ed Gag proteins (Bryant et al. 1989; Bryant et al. 1991) . Of the heteroatom substituted analogs, 13-OxaMyr is the most potent inhibitor. 13-OxaMyr is added to Gag with an effi cacy similar to that of myristate and alters viral polyprotein processing, which is suggested to be a consequence of inhibiting Gag and Ribosomal frameshifting 1,4-bis-[N-(3-N,N Small molecule (Hung et al. 1998) . dimethylpropyl)amidino]benzene tetrahydrochloride (RG501) Myristoylation and Traffi cking 12-azidododecanoic acid Myristic acid analogue ) 4-oxatetra-decanoic acid (Langner et al. 1992) 12-methoxydodecanoate Heteroatom-substituted (Bryant et al. 1989 ) 5-ocytl-oxypentanoate myristic acid analogues (Bryant et al. 1991 ) 11-ethylthioundecanoic acid (Parang et al. 1997 ) 12-thioethyldodecanoic acid 5-cis-tetradecenoic acid Unsaturated 14-Carbon (Lindwasser and Resh, 2002) . (physeteric acid) fatty acids 5-cis,8-cis-tetradecenoic acid (goshuyic acid) Small molecule (Tang et al. 2003) PAATLEEMMTA CA derived peptide (Niedrig et al. 1994 ). GPG-NH 2 CA derived tripeptide amide (Hoglund et al. 2002) CAI Peptide ) (Ternois et al. 2005) Maturation-Gag processing 3-0-(3′-3′-dimethylsuccinyl)-betulinic Small molecule (Li et al. 2003 ) acid (Zhou et al. 2004 ) (PA-457/bevirimat) electrophilic disulfi de-substituted NC Zn fi nger inhibitor (Rice et al. 1995 ) benzamides (DIBAs) (Turpin et al. 1996) 1,2-dithiane-4,5-diol,1,1-dioxide NC Zn fi nger inhibitor (Rice et al. 1997 ) (NSC 624151) S-acyl 2-mercaptobenzamide thioester NC Zn fi nger inhibitor (Schito et al. 2006 ). (SAMT) Ac-TLNF-OH PR C-terminal tetrapeptide (Zhang et al. 1991) Pal-YDL-OH Modifi ed PR C-terminal (Schramm et al. 1999 ) Pal-YD-(biphenylalaine)-OH lipopeptides Pal-YDT-OH Apam(2)-YD-thyroxine-OH (Dumond et al. 2003) PQITL(GGG)CTLNF Glycine linked PR interface (Babe et al. 1992 ) tetra-peptides HO-FNLTS-NH-(CH 2 ) n -N-PQITLW-OH Alkyl linked PR interface (Zutshi et al. 1997 ) peptides (Ulysse and Chmielewski, 1998 ) (Zutshi and Chmielewski, 2000) (Continued) Scaffold constrained PR (Bouras et al. 1999 ) interface peptides (Breccia et al. 2003 ) (Merabet et al. 2004 Didemnaketal A analogue (Fan et al. 1998) Ursolic Acid Triterpene (Quere et al. 1996) NHGRNLLTQI (S8) PR LES peptide (Broglia et al. 2005 ) (Broglia et al. 2006) IVQVDAEG (p51) Random peptide (Park and Raines, 2000) . Vpr-(spacer)-TLNF-OH Vpr, PR C-terminal fusion peptide (Cartas et al. 2001) . RT connection subdomain peptide (Morris et al. 1999 ) (Depollier et al. 2005) TLMALELKGKLLLAGLAPSAFLPLSFP Designed peptide targeting (Campbell et al. 2002 RT connection subdomain (Hosokawa et al. 2004 ) INI 1 Host cell factor (Yung et al. 2001 ) (Sorin et al. 2006 ) (Ariumi et al. 2006 ) (Kalpana et al. 1994) Budding and Egress 5-(N,N-hexamethylene)amiloride (HMA) Amiloride analogue (Ewart et al. 2002) 5-(N,N-dimethyl) Gag-Pol traffi cking (Bryant et al. 1991) . In an acutely infected T-cell line 13-OxaMyr reduces HIV-1 replication in the 40-80 µM range (Bryant et al. 1989) . 13-OxaMyr also inhibits viral production in chronically infected H9/IIIB cells, which is consistent for an inhibitor that targets the late stage of HIV-1 replication (Bryant et al. 1991) . 13-OxaMyr exhibits a synergistic anti-HIV-1 effect with AZT suggesting its potential for use in combination therapy (Bryant et al. 1991) . The therapeutic effi cacy of 13-OxaMyr can be further enhanced by conjugation with glycerophospholipid L-∝-phosphatidylethanolamine (Pidgeon et al. 1993) . The selectivity of these heteroatom analogs for the target protein is dependent on the position of the substituted heteroatom, thus they can be exploited as a therapeutic antiretroviral strategy. Nevertheless, heteroatom-substituted myristic acid analogs are still expected to adversely affect a substantial range of cellular processes that depend on protein N-myristoylation (Lindwasser and Resh, 2002 ). An alternative strategy for targeting Gag myristoylation is the exogenous treatment of cells with unsaturated 14-carbon fatty acids including 5-cistetradecenoic acid (14:1n-9, physeteric acid) and 5-cis,8-cis-tetradecadienoic acid (14:2n-6, goshuyic acid) (Lindwasser and Resh, 2002) . As lipid rafts have preference for saturated fatty acids, treatment with unsaturated analogs interferes with membrane targeting of Gag and consequentially viral assembly and production (Lindwasser and Resh, 2002) . These inhibitors also interfere with certain Srckinase mediated cellular pathways, although they appear to have no effect on cell proliferation (Campbell and Vogt, 1995) . It is suggested that direct dietary intake of physeteric acid and goshuyic acid could be a useful therapeutic strategy for the treatment of HIV-1 infections. However, the effect of long term intake of these unsaturated fatty acids and their effect on N-myristoylated signaling proteins such as Src, G-proteins, Arf and heterogeneously N-acylated retinal proteins needs to be assessed (Lindwasser and Resh, 2002) . Recent studies also indicate a role for phosphatidylinositide 4,5-bisphosphate [PI(4,5)P2] in regulating Gag localization (Ono et al. 2004 ). In HIV-1, binding of PI(4,5)P2 to the MA domain in Gag activates the "myristyol switch" and also acts as the point of membrane attachment (Saad et al. 2006) . The binding site of PI(4,5)P2 on MA is highly conserved amongst HIV-1 strains and therefore represents an attractive antiviral target (Shkriabai et al. 2006; Saad et al. 2006 ). CA plays an important role in the HIV-1 life-cycle by promoting Gag-Gag interactions during virion maturation. The N-and C-terminal domains of this protein serve distinct functions. As shown by mutational analysis, the N terminal domain of CA (N-CA), otherwise known as the NTD, is responsible for maintaining the proper conformation of CA during the assembly process (Worthylake et al. 1999; Li et al. 2000) . The C-terminal domain of CA (C-CA) or the CTD, is critical for Gag-Gag interactions during assembly and maturation (Gamble et al. 1996; Gamble et al. 1997 ) and described mutations in this region have major consequences on virion maturation and infectivity (von Schwedler et al. 2003; Ganser-Pornillos et al. 2004 ). The NMR structure of CA has demonstrated that the protein consists mainly of seven α-helices, two β-hairpins and a loop structure (Momany et al. 1996; Gitti et al. 1996) . Five of the α-helices form a coiled-coiled structure while one of the β-hairpins is located on the surface of the N-terminal domain of the protein (Momany et al. 1996) . The second β-hairpin is predicted to be formed after cleavage by the HIV-1 PR (Tang et al. 2002) . Cleavage of CA from its neighbouring proteins is necessary for core condensation and conical capsid shell formation (Vogt, 1996; Wiegers et al. 1998) . Compounds that bind to these regions would be expected to disrupt proper CA shell formation and virion infectivity making CA an important and attractive target for the development of antiretroviral agents. A proof of concept study, demonstrating the potential of inhibiting CA-CA interactions as an antiretroviral target has been published (Tang et al. 2003) . Computational high throughput screening of a small molecule library and NMR analysis for binding specifi city resulted in the identifi cation of CAP-1 and CAP-2 which bind to an apical site on the NTD of both immature and mature CA (Tang et al. 2003) . While CAP-2, is toxic to U1 cells, CAP-1 reduces viral infectivity by 95% at 100 µM. The released virions lack cone shaped cores and resemble viral particles that have been observed in HIV-1 expressing mutations that disrupt CA-CA interactions (Dorfman et al. 1994; Reicin et al. 1996; von Schwedler et al. 2003; Lanman et al. 2003) . Despite aberrant viral morphology CAP-1 does not affect viral particle release or proteolytic processing (Tang et al. 2003) . CAP-1 and CAP-2 bind to a common site within the NTD thus preventing CA-CA interactions and proper Gag assembly. Peptides derived from HIV-1 CA have also been described to affect viral morphogenesis by interfering with capsid formation (Niedrig et al. 1994) . The synthetic peptide, PAATLEEMMTA, inhibits HIV-1 replication in cell culture assays at 20-200 µg/ml and results in the production of immature and aberrant viral particles (Niedrig et al. 1994) . Tripeptide amides derived from the carboxyl terminus of CA inhibit HIV-1 replication, with the three most potent peptides interacting with CA as demonstrated by capillary electrophoresis analysis (Hoglund et al. 2002) . Glycyl-prolyl-glycine-amide (GPG-NH 2 ) interferes with the formation of HIV-1 particles with a normal conical core structure (Hoglund et al. 2002) . G-NH 2 is an active metabolite of GPG-NH 2 indicating that the latter acts as a pro-drug (Andersson et al. 2005) . However, the development of HIV-1 resistance to either G-NH 2 or GPG-NH 2 has been elusive suggesting that the peptides mediate their effects through a host cell or other factor (Andersson et al. 2004 ). CAI, a small peptide selected by phage display screening, acts as an inhibitor of the assembly of immature Gag in vitro Ternois et al. 2005) . CAI binds to the C-terminus of CA (K d ~ 800 µM), thus preventing the necessary conformational changes in CA that lead to the formation of mature cores . The structure of CAI complexed with CA has revealed that the CAI binding region is a highly conserved hydrophobic pocket within the C terminus of CA where the peptide forms an extra α-helix, which binds to the four α-helices of CA (Ternois et al. 2005) . The resulting proteinpeptide complex is therefore a fi ve α-helix bundle with reduced CA-CA dimerization contacts that destabilizes the dimer interface. Binding of CAI to the C-CA not only affects the assembly of the immature capsid particles but also reduces the amount of correctly assembled mature capsids in vitro, thus acting as a promising two-step inhibitor . The C-terminal domain of Gag in the context of Gag-Pol is essential for its interaction with Gag and its incorporation into the virion (Srinivasakumar et al. 1995; Chiu et al. 2002; Chien et al. 2006) . This sequence includes a highly conserved "major homology region" (MHR) in the CA domain of Gag and the adjacent CA-SP1 (Srinivasakumar et al. 1995; Chien et al. 2006) . These sequences are also critical for HIV-1 Gag assembly as they drive Gag oligomerization. However, the magnitude of the virion incorporation defect of Gag-Pol MHR deletion mutants varies between different studies making the value of targeting this region of Gag-Pol unclear with respect to inhibition of the late stages of HIV-1 replication (Mammano et al. 1994; Srinivasakumar et al. 1995; Chiu et al. 2002; Chien et al. 2006) . Sequences involved in Gag and Gag-Pol interactions are assumed to be similar to those involved in Gag-Gag interactions. However, virions generated in the presence of CAP-1 are unlikely to affect Gag/Gag-Pol interactions as defects in proteolytic processing in the virus or virion associated RT activity were not observed (Tang et al. 2003) . The proline rich region of p6 has also been implicated in the packaging of cleaved Pol proteins into virions, which is suggested to be mediated by host cell proteins (Dettenhofer and Yu, 1999; Cen et al. 2004) . Identifying the host cell factor implicated in the virion incorporation of cleaved Pol will be necessary for establishing this process as a viable drug target. The HIV-1 NC (NCp7) contains two highly conserved zinc fi nger motifs C-X 2 -C-X 4 -H-X 4 -C (X, any amino acid). The zinc fi ngers of NC are critical in the early and late stages of HIV-1 replication with mutations in the zinc chelating amino acids resulting in formation of noninfectious virus (Aldovini and Young, 1990) . The zinc fi ngers of NCp7 are required for initiation, elongation and effi cient template switching during reverse transcription (Rodriguez-Rodriguez et al. 1995; Tanchou et al. 1995) . NCp7 is also involved in HIV-1 genomic RNA dimerization, IN cleavage activity and coats the viral RNA genome protecting it from nucleases (Lapadat-Tapolsky et al. 1993) . Given the critical role of NCp7 zinc fi ngers in HIV-1 replication it is not surprising that agents that covalently modify the zinc chelating residues of NCp7 have been described as inhibitors of HIV-1 replication (Rice et al. 1995) . The electrophilic disulfi de-substituted benzamides (DIBAs) inactivate cell free virus and inhibit the early and late stages of HIV-1 replication by interfering with reverse transcription and viral particle maturation (Rice et al. 1995; Turpin et al. 1996) . In the U1 cell line treatment with DIBAs results in the inhibition of virus particle release, processing of Gag, and the production of virions with reduced infectivity (Turpin et al. 1996) . The defect in viral particle release and maturation was attributed to the formation of intermolecular cross-linkages between the zinc fi ngers of adjacent Gag molecules, thereby preventing effi cient cleavage by the HIV-1 PR (Turpin et al. 1996) . The non-dissociable tethered dithiane compound 1,2-dithiane-4,5-diol,1,1-dioxide, (NSC 624151) also mediates similar defects in Gag processing (Rice et al. 1997) . Although cellular proteins also contain zinc fingers, these inhibitors appear to preferentially target retroviral zinc fingers. This may be explained by the inaccessibility of these inhibitors to the appropriate cellular compartments where zinc finger containing cellular proteins are located. The in vivo anti-HIV-1 activity of zinc finger inhibitors has been demonstrated in a transgenic murine model where infectious HIV-1 is induced from an integrated provirus (Schito et al. 2003) . A recent study in a nonhuman primate model demonstrated a reduction in the levels of SIV/ DeltaB670 in peripheral blood mononuclear cells during therapy with the zinc finger inhibitor, S-acyl 2-mercaptobenzamide thioester (SAMT), although there was no effect on viral load (Schito et al. 2006) . Further studies are in progress to optimise the bioavailability and pharmacokinetics of this promising inhibitor. Much of our understanding of how the PR domain in Gag-Pol is activated and the processing cascade of Gag and Gag-Pol are due to the contributions of Kaplan and colleagues Pettit et al. 2005) . Strict regulation of PR function is critical for effi cient production of mature viral particles. Premature activation, partial inhibition, or over-expression of HIV-1 PR leads to major defects in viral assembly and the production of non-infectious viral particles (Krausslich, 1991; Kaplan et al. 1993; Karacostas et al. 1993) . Hence novel inhibitors designed to prevent or perturb PR dimerization could potentially inhibit the mature PR homodimer and the immature Gag-Pol embedded PR. HIV-1 PR is a homodimeric aspartyl protease formed by the symmetrical association of two 99 amino acid subunits. The crystal structure reveals a compact, predominantly β-strand structure with a short α-helix region near the C terminus (Wlodawer et al. 1989 ). Dimerization of the PR monomers generates both the substrate-binding pocket and the catalytic centre and is essential for PR activity (Cheng et al. 1990 ). The PR dimer has a dissociation constant of 50 nM and Gibbs free energy of dimer stabilisation of 10 kcal/mol (25 o C, pH 3.4). Nearly 75% of the binding energy is contributed by the four-stranded β-sheet formed by the N-and C-termini (Todd et al. 1998 ).The four-stranded β-sheet comprising the N-and C-termini from each PR monomer represents an attractive drug target for the following reasons: 1. It is the major stabilising region of the active dimer, 2. The region is relatively free of known PR resistance mutations, 3. The sequence is highly conserved in most HIV-1 and HIV-2 isolates and 4. It provides a unique target minimising potential toxicity issues for eukaryotic aspartyl proteases (Gustchina and Weber, 1991) . A standard methodology for analysing potential PR inhibitors that prevent PR dimerization (dissociative inhibition) or target and bind to the PR active site (competitive inhibition) has been described (Zhang et al. 1991 ). An example of a dissociative inhibitor is the C-terminal tetrapeptide, Ac-T-L-N-F, which exhibits activity in the micromolar range (K i 45 µM) (Zhang et al. 1991 ). Other studies have also shown the capacity of N-and C-terminal peptides, or 'interface' peptides, to bind to PR monomers and thus prevent PR dimerization and activity Franciskovich et al. 1993; Schramm et al. 1991; Schramm et al. 1996) . The identifi cation of these lead peptides provides proof of concept that targeting the PR β-sheet region constitutes a viable strategy for the development of novel inhibitors of HIV-1 PR. The potency of C-terminal tetrapeptides are increased by truncation to a core tripeptide, amino acid modifi cation, and the addition of a linear hydrophobic moiety such as palmitoyl to the amino terminus of the peptide. The lipid moiety is thought to increase the dissociative activity of the peptides by directing it to the hydrophobic PR interface (Schramm et al. 1999) . However, despite their capacity to inhibit PR activity at low nanomolar concentrations, these lipopeptides are poorly soluble and susceptible to protein degradation. Further modifi cations have been made to the lipopeptides by making them less peptide-like (Cafl isch et al. 2000) and by modifying the lipid moiety to increase their solubility while retaining potency (Dumond et al. 2003) . Cross-linking interfacial peptides represent another strategy, with the aim to increase the affi nity of the peptides by presenting them in a conformation similar to a PR monomer. The fi rst interface tetrapeptides tethered with a glycine linker display greater potency (PF1, IC 50 = 40 µM) compared to free tetrapeptides (IC 50 ≥ 150 µM) (Babe et al. 1992) . This approach has evolved to linking peptides with fl exible alkyl tethers (Zutshi and Chmielewski, 2000) and semirigid alkyl based tethers (Ulysse and Chmielewski, 1998) , which increase the distance between the peptides to approximate that of the PR termini in the dimer (~10 Å). The conformational freedom of these linked peptides was addressed by the use of pyridinediol and naphthalene based molecularly constrained scaffolds (Bouras et al. 1999; Song et al. 2001; Merabet et al. 2004; Bannwarth et al. 2006) . Known as 'molecular tongs', these compounds are designed to position the interface peptides to clamp the termini of a PR monomer (Fig. 2) . These studies have culminated in a set of optimised tongs with symmetrical peptidomimetic sequences based on an optimised PR C-terminal sequence. The tongs inhibit the activity of HIV-1 PR that are either sensitive or resistant to PIs with Ki values from 0.4-4.8 µM in cell free assays (Bannwarth et al. 2006) . Other variations on the theme of interface peptides include combining the advantages of lipopeptides and molecular tongs. Interface peptides have been linked to lipophilic groups by a rigid bicyclic guanidinium scaffold (Breccia et al. 2003) . The most potent compound demonstrates PR inhibitory activity similar to tethered peptides and molecular tongs. Cross-linked interfacial peptides have been designed to irreversibly inhibit HIV-1 PR by formation of a disulfi de bond between the peptide and the conserved PR residues C-95 and C-67, and demonstrate a K i in the low micromolar range (Zutshi and Chmielewski, 2000) . The C-terminal tetrapeptide has also been tethered to a peptidic PR active site inhibitor, combining both dissociative and competitive methods of inhibition in one molecule (Uhlikova et al. 1996) . Random peptides that are dissociative inhibitors of HIV-1 PR have been described. The bacteriophage lambda repressor protein was utilised to develop a powerful two-hybrid PR dimerization assay. From a library of 5 × 10 8 random peptides, 300 were identifi ed as potential PR dimerization inhibitors. The most potent peptide identifi ed, p52, was a pure dissociative inhibitor with low K i of 780 nM (Park and Raines, 2000) . Ultimately, one of the major hurdles in developing peptidic inhibitors is to obtain a biologically stable compound that can be delivered inside the cell. One mechanism to achieve this is to fuse the peptide to amino acid sequences that promote either encapsidation into viral particles or entry into the host cell. Virus protein R (Vpr) is a HIV-1 accessory protein packaged into virions by its trans association with the Gag p6 motif. Both viral and cellular proteins have been successfully delivered into viral particles as Vpr fusion proteins (Wu et al. 1997 ). Inhibition of HIV-1 replication has been reported by the fusion of Vpr to viral PR recognition sequences (Serio et al. 2000) . Expression of the PR C-terminal tetrapeptide as a Vpr fusion [Vpr-(spacer)-T-L-N-F-OH] attenuates HIV-1 replication in chronically infected cells and in single-round replication assays (Cartas et al. 2001) . Most recently, inhibition of HIV-1 replication has been demonstrated by delivering PR interface peptides as a fusion peptide utilising the HIV-1 Tat derived cell permeable protein transduction domain (Davis et al. 2006) . Peptides P27/A are PR dimerization inhibitors that inhibit the activity of wild-type and drug resistant PR in cell free assays with IC 50 values in the 0.28-0.58 µM range. These peptides are successfully delivered into chronically HIV-1 infected cells and reduce viral particle production. This was observed by a reduction in p24, rather than inhibition of Gag processing which suggests that the peptide may interact with the Gag-Pol embedded PR and disrupt the ordered processing of Gag-Pol leading a decrease in viral particle production (Davis et al. 2006 ). Local elementary structures (LES) are comprised of strongly interacting, highly conserved amino acids that are usually hydrophobic. These amino acids are suggested to direct the folding of a protein into its native conformation. Short peptides corresponding to or mimicking the LES are hypothesised to act as folding inhibitors, preventing the protein achieving its native conformation (Broglia et al. 2005) . A peptide has been identifi ed from a LES in the HIV-1 PR (peptide S8, amino acids 83-93) that inhibits PR activity with a K i of 2.58 µM and results in disorganisation of the PR secondary structure by reducing the β-sheet content from 30% to 14% (Broglia et al. 2005; Broglia et al. 2006) . Current efforts are directed towards developing a shorter less hydrophobic peptide or mimetic based on the S8 lead peptide. Catalytically inactive PR monomers act in a dominant negative fashion to inhibit wild-type HIV-1 PR by forming inactive heterodimers in recombinant protein assays . When virus expressing a PR active site mutation is co-transfected with wild-type HIV-1, both viral replication and virus infectivity are reduced (Babe et al. 1995) . Computer modelling has been used to successfully design an optimised dominant-negative PR expressing D25K, G49W and I50W (KWW) , which also reduces viral replication and infectivity (Junker et al. 1996) . Biochemical studies on recombinant dominant negative PRs confi rm that the mechanism of action is by formation of inactive heterodimers (Rozzelle et al. 2000) . Interestingly, the mutant PRs cannot homodimerize and they fold only when expressed with wild-type PR. PR heterodimers are also more stable that the wild-type homodimer (Rozzelle et al. 2000) . Hence inactive PR heterodimers form the dominant species. Such a dominant negative strategy for the inhibition of HIV-1 PR would require in vivo delivery by a genetherapy system, the therapeutic use of which is unlikely in the near future. A screen of a crude extract from the marine organism magenta ascidian didemnum identifi ed two didemnaketals, A (a bicyclic ketal) and B (a linear heptaprenoid), that inhibit HIV-1 PR activity with IC 50 values of 2 µM and 10 µM, respectively (Potts et al. 1991) . These compounds are unsuitable drug candidates, but have given rise to a novel class of pentaesters, the most potent of which is a dissociative inhibitor of PR with a K i of 2.1 µM (Fan et al. 1998) . A novel class of PR dimerization inhibitors were identifi ed by searching the Cambridge structural database for pharmacophores that mimic the action of previously identified inhibitory interface peptides (Quere et al. 1996) . Several triterpene structures were identifi ed, of which ursolic acid acts as a dissociative inhibitor of PR with an IC 50 of 2 µM. It has been suggested that triterpene could provide another basic scaffold for building more effective peptidomimetics. Interestingly, another member of the triterpene family, PA-457, acts as a novel inhibitor of HIV-1 maturation which is discussed later in this review. A β-sheet mimetic was tested for its ability to inhibit PR homodimerization by perturbation of β-sheet formation (Song et al. 2001) . The β-sheet mimetic had a relatively high IC 50 of 30 µM and the method of inhibition appears to be complex, however the structure provides a non-peptidic lead compound for PR inhibitors. The rate and the specifi city of Gag cleavage by the HIV-1 PR is dependent on the amino acid composition of the different cleavage sites recognized by the viral PR (Swanstrom, 1997) . Based on the order of proteolysis by HIV-1 PR, these sites are classified as primary (p2/NC), secondary (MA/CA and p1/p6) or tertiary (CA/p2 or CA/SP2) cleavage sites. The lack of processing of any of these sites by PR results in the formation of aberrant particles (Swanstrom, 1997) . In particular, inhibition of cleavage at the CA/p2 site has severe consequences for core formation, stability and virion infectivity (Pettit et al. 1994; Wiegers et al. 1998; Pettit et al. 1998; Shehu-Xhilaga et al. 2001b ). The α-helical structure that stretches between the C-terminus of CA and the N-terminus of SP1 is critical for virion assembly and p2 function (Accola et al. 1998) . Clearly, these PR cleavage sites are potential targets for antiretroviral drug design. In this regard, a compound that interferes with viral maturation by blocking CA/p2 cleavage has been identifi ed. 3-O-(3′,3′-Dimethysuccinyl) betulinic acid (PA-457 or bevirimat) potently inhibits HIV-1 maturation and infectivity (Li et al. 2003; Zhou et al. 2004 ). PA-457 specifi cally blocks the cleavage of CA/p2 in cell based (Li et al. 2003) and in cell free assays (Zhou et al. 2005; Sakalian et al. 2006) , thus inhibiting core condensation and virion maturation. Inhibition of Gag processing at the CA/p2 junction results in the generation of the uncleaved p25 product in transfected cells at 0.1 µg/ml of PA-457 (Li et al. 2003) . Consistent with the proposed mechanism, PA-457 resistant HIV-1 selected in long term cultures in the presence of betulinic acid contain mutations in the regions that fl ank the P-P' scissile bond Zhou et al. 2006 ). These mutated sites in Gag are recognized by the viral PR during proteolysis. In addition, other single amino acid substitutions have been identifi ed that confer resistance to PA-457 and are exclusively located either at the C terminus of CA or within the fi rst three amino acids of the p2 spacer peptide . Interestingly, they all conferred resistance independently and were located within the boundaries of the CA/p2 proteins, a region well known to promote Gag multimerization . These data suggest that there is more than one mechanism by which these mutants have acquired resistance to PA-457. PA-457 has successfully undergone Phase 1 and 2 clinical trials and is currently in a Phase 2b trial to test the effi cacy of different doses of PA-457 in combination with approved HIV-1 inhibitors as part of an optimised regimen in patients failing therapy due to the emergence of drug resistant virus. Like other HIV-1 enzymes, RT subunits must oligomerize to form an active enzyme. The biologically relevant form that is present in the virion is an asymmetric heterodimer comprised of the p66 (66 kDa) and the p51 (51 kDa) subunits (Jacobo-Molina et al. 1993; Kohlstaedt et al. 1992) . The RT heterodimer is extremely stable and has an extensive protein surface area (4800 Å 2 ) that is buried upon subunit dimerization. Thermodynamic measurements of the association between the p66 and p51 RT subunits have estimated Gibbs free energy of dimer stabilization of approximately 10-12 kcal/mol -1 , corresponding to a dissociation constant of approximately 3 × 10 -7 M (Venezia et al. 2006) . For an extensive review on HIV-1 RT dimerization see Srivastava et al. 2006 . Regions both upstream and downstream of the PR region in Gag-Pol have been investigated for effects on PR activation (Bukovsky and Gottlinger, 1996; Partin et al. 1991; Louis et al. 1999 ). Large deletions within or C-terminal truncations of RT in the context of Gag-Pol result in an increase in virion associated Gag processing intermediates, suggesting a defect in PR activity (Cherry et al. 1998; Liao and Wang, 2004; Quillent et al. 1996) . These studies suggest that modulating RT dimerization in the context of Gag-Pol may have a negative impact on PR activation and HIV-1 maturation. The importance of the RT region in Gag-Pol for both RT maturation and viral particle production has been demonstrated by the study of RT point mutations that prevent RT heterodimerization and p66 homodimerization. Mutations at W401, a component of the highly conserved tryptophan repeat motif in the connection subdomain, blocks RT dimerization in vitro (Tachedjian et al. 2003; Tachedjian et al. 2005b) . When expressed in HIV-1 it manifests as defects in reverse transcription, aberrant processing of RT, and low levels of infectivity (Wapling et al. 2005) . The L234A mutation, located in the RT primer grip region, prevents RT dimerization and decreases Gag-Pol stability (Tachedjian et al. 2000; Yu et al. 1998 ). This mutation reduces PR incorporation into virions, increases the accumulation of Gag processing intermediates, and results in the production of non-infectious virus particles (Tachedjian et al. 2000; Yu et al. 1998) . These examples demonstrate the potential for targeting the Gag-Pol embedded RT for blocking HIV-1 maturation. Apart from classical NNRTIs, there exists a class of unconventional NNRTIs that bind to HIV-1 RT and inhibit enzyme activity by decreasing the overall stability of the heterodimer without dissociating the complex (Sluis- Cremer et al. 2000; Sluis-Cremer and Tachedjian, 2002; Camarasa et al. 2006 ). The TSAO-T derivatives ([2′,5′-bis-O-(tert-butyldimethylsilyl)-β-D-ribofuranosyl]-3′spiro-5″-(4″-amino-1″,2″-oxathiole-2″,2″-dioxide) thymine) destabilise both RT heterodimers and p66 homodimers by inducing changes at the RT dimer interface (Sluis- Cremer et al. 2000; Rodriguez-Barrios et al. 2001) . The putative binding site is at the RT dimer interface and overlaps in part with the NNRTI binding pocket (Rodriguez-Barrios et al. 2001) . Resistance mutations to the TSAO drugs are readily generated in cell culture indicating that these drugs are specific inhibitors of the HIV-1 RT (Balzarini et al. 1993) . While TSAO represent the fi rst class of small molecules that destabilize the RT heterodimer, preclinical studies demonstrate that the pharmacological profile of TSAO inhibitors is unfavourable for further clinical development . The N-acylhydrazone derivative N-(4-tert-Butylbenzoyl)-2-hydroxy-1-naphthaldehyde hydrazone (BBNH) binds to both the DNA polymerase and RNase H domains of RT and inhibits both enzymatic activities of the RT (Arion et al. 2002) . Similar to TSAO, BBNH prevents RT activity through destabilizing, but not dissociating the subunits. BBNH derivatives that bind to the DNA polymerase domain alone are suffi cient to induce dimer destabilization (Sluis- Cremer and Tachedjian, 2002) . The recently resolved structure of HIV-1 RT bound to a BBNH derivative has confi rmed that the binding site is in close proximity to, but distinct from both the polymerase active site and NNIBP. It is thought that BBNH destabilizes the RT heterodimer by inducing changes in the primer-grip motif, which is an important region for RT dimer stability (Himmel et al. 2006; Srivastava et al. 2006) . By targeting the RT dimerization interface, it is possible that unconventional NNRTIs may have an affect on the late stage of virus replication. In particular, the TSAO drugs that destabilize the p66 homodimer, may also perturb the process of RT maturation to the heterodimer, and arguably even target the RT domain in Gag-Pol and interfere with PR activation. However, these possible effects have not been described. Further elucidation of the impact of nonclassical NNRTIs on RT maturation, and the mechanism of destabilization would be advantageous for designing more potent inhibitors of both RT function and RT maturation. Interestingly, several classical NNRTIs have been shown to confer a concentration dependant increase in RT heterodimer formation, corresponding with a loss of RT polymerase function (Tachedjian et al. 2001; Venezia et al. 2006 ). Efavirenz (EFV) is a strong enhancer of RT dimerization, and also enhances the formation of p66 and p51 homodimers (Tachedjian et al. 2005a ). The exact mechanism for increasing RT subunit interactions is unknown but it is suspected that the binding of EFV to the RT mediates conformational changes in the p66 subunit that promotes interaction with p51 (Tachedjian et al. 2001 ). EFV has also demonstrated the capacity to enhance the homodimerization of p66 in vitro, and a 90kDa model Pol protein in an inducible bacterial expression system (Sluis- Cremer et al. 2004; Tachedjian et al. 2005a) . It has recently been demonstrated that NNRTIs enhance Gag-Pol dimerization, resulting in premature PR activation and a decrease in viral particle release (Figueiredo et al. 2006 ). In HIV-1 transfected cells, EFV, TMC120 and TMC125 increased Gag and Gag-Pol processing, and caused up to 45% decrease in viral particle production. Similar effects were not observed for NNRTIs that do not signifi cantly enhance p66 homodimerization and NRTIs (Figueiredo et al. 2006) . Hence, NNRTIs that are potent enhancers of RT dimerization also affect the late stage of viral replication, which represents a novel inhibitory mechanism for these drugs. However, the concentrations required to mediate this effect are two to three orders of magnitude higher then concentrations that block RT function. This is likely due to reduced binding affi nity of the NNRTIs to the proposed target which is the RT domain of Gag-Pol. Strategies to identify drugs that are more potent inhibitors of this late stage in the viral life-cycle could be identifi ed by screening for molecules that enhance Gag-Pol dimerization. Such a screen could be enhanced by incorporating mutations in the RT that are known to confer decreased susceptibility to current NNRTIs in order to select for drugs that have the potential to block NNRTI resistant strains of HIV-1. Two strategies have been utilised to generate peptides designed to target the RT dimer interface in order to block HIV-1 RT function, including peptides corresponding to regions that are known to have an important role in RT dimerization (Debyser and De Clercq, 1996; Depollier et al. 2005; Divita et al. 1994; Morris et al. 1999b; Morris et al. 1999a) . The most successful of these peptides, Pep-7, corresponds to RT residues 395-404, derived from the highly conserved tryptophan repeat motif (W398-W414) (Depollier et al. 2005) . Pep-7 interacts with p51, and destablizes both the RT heterodimer and the p66 homodimer. Similar to unconventional NNRTIs, Pep-7 is unable to induce RT dissociation (Depollier et al. 2005) . Pep-7 based peptides are potent suppressors of HIV-1 replication at noncytotoxic concentrations (Morris et al. 1999b ). The method of inhibition in HIV-1 infected cells has not been elucidated. However, given that Pep-7 cannot induce RT subunit dissociation, it has been suggested that it acts at the late stage of virus replication by preventing the formation of an active RT heterodimer (Morris et al. 1999b) . Rational strategies utilising the available RT structures to direct the design and manufacture of mimetic peptides targeting subunit interaction is a recent development (Campbell et al. 2002; Hosokawa et al. 2004 ). These studies have led to the synthesis of a peptide, TLMA2993, which also targets the RT connection subdomain. TLMA2993 inhibits RT activity at micromolar concentrations (Campbell et al. 2002) . Cells stably transfected with this peptide are protected from HIV-1 infection in a concentration dependant manner due to inhibition of reverse transcription, as observed by a decrease in HIV-1 DNA (Hosokawa et al. 2004 ). IN catalyses the insertion of viral DNA into the host chromosome and thus inhibits an early crucial step in the virus life cycle. IN is also implicated in reverse transcription, nuclear import of the pre-integration complex, viral assembly and budding (Engelman et al. 1995; Hehl et al. 2004 (Cotelle, 2006; Makhija, 2006) and have shown effi cacy in Phase III clinical trials (Stephenson, 2007) . Since IN is expressed as part of Gag-Pol, agents that bind to the IN domain in this polyprotein are likely to impact on the late stages of replication. Consistent with this notion, mutations in IN have been reported to effect virion formation (Shin et al. 1994) . Truncations of IN at the C-terminus of Gag-Pol result in aberrant virion core structures, with a reduction in the overall levels of cell-associated viral Gag, suggesting a defect in Gag-Pol processing (Engelman et al. 1995; Bukovsky and Gottlinger, 1996) . IN requires oligomerization for activity. Therefore, inhibitors of IN function that mediate their effects through negating IN subunit interactions are also likely to interfere with viral assembly. This would be manifested by interfering with Gag-Pol/Gag-Pol interactions leading to subsequent effects on HIV-1 PR activation (Muriaux et al. 2004) . In this regard peptide inhibitors of IN dimerization have been reported, however their effects on the late stages of the virus life-cycle remains to be determined (Maroun et al. 2001; Zhao et al. 2003) . Certain host cell factors are incorporated into the virion by interaction with the IN domain of Gag-Pol. A cellular factor that has been implicated in affecting the late stage of the virus life cycle is integrase interactor 1 (INI1). INI1 was identifi ed in a yeast two-hybrid screen for host cell proteins interacting with HIV-1 IN (Yung et al. 2001; Yung et al. 2004; Kalpana et al. 1994) . INI1 mutants that abrogate interaction with IN or cells defi cient in INI1 exhibit a substantial reduction in viral production (Yung et al. 2001) . INI1 affects several steps during HIV-1 replication (Ariumi et al. 2006; Sorin et al. 2006; Yung et al. 2001) and is also packaged into HIV-1 particles (Kalpana et al. 1994) . A 110amino-acid fragment of INI1 (S6) with a minimal IN-interaction domain inhibits viral production (Yung et al. 2001; Yung et al. 2004) . The inhibitory effect of S6 on HIV-1 production is mediated by binding of the ectopically expressed S6 to the Gag-Pol embedded IN. Furthermore, stable expression of a transdominant S6 mutant inhibits infection in T-cells. S6 represents a potential lead for the development of inhibitors of the late stage of HIV-1 replication (Yung et al. 2001 ). Intracellular degradation of misfolded, damaged or unwanted proteins is mediated by the proteosome, which is a multisubunit proteolytic complex of 26S (Schubert et al. 2000) . Proteins are tagged for proteolytic destruction by the covalent attachment of a chain of ubiquitin polypeptides on lysine residues of the protein (Schubert et al. 2000) . Proteosome inhibitors inhibit the late stages of the HIV-1 life-cycle by interfering with viral particle release and maturation (Schubert et al. 2000) . Decreased budding has also been demonstrated for retroviruses expressing the PPPY-or PTAP containing late domains but not those that use the YPDL type late domain (Schubert et al. 2000; Ott et al. 2003 ). The effect is not dependent on the viral particle assembly site (i.e. cytoplasm or plasma membrane) or on monoubiquitination of Gag (Ott et al. 2003) . In addition to a decrease in viral particle release (4-fold), virions released from cells treated with proteosome inhibitors have approximately a 10-fold decrease in infectivity (Schubert et al. 2000) . The impact of proteosome inhibitors is dependent on an active HIV-1 PR and the presence of the p6 late domain but is independent of Vpu function. Inhibition of HIV-1 maturation and budding is observed with reversible (zLLL also known as MG-132) and irreversible (lactocystin) proteosome inhibitors (Schubert et al. 2000) . Proteosome inhibitors also interfere with the activity of the HIV-1 viral infectivity factor (Vif) on the antiviral function of apolipoprotein B mRNAediting enzyme catalytic polypeptide-like 3G (APOBEC3G) in virus producing cells (Stopak et al. 2003; Sheehy et al. 2003; Mehle et al. 2004; Yu et al. 2003) . Wild-type viruses expressing Vif are able to prevent incorporation of APOBEC3G into the virion by promoting its degradation in the cytoplasm of the producer cell. Inhibition of APOBEC3G incorporation in the virus prevents hypermutation in newly synthesized viral DNA following infection of target cells due to C to U modifi cations during minus stand DNA synthesis mediated by APOBEC3G. Proteosome inhibitors interfere with Vif dependent degradation of APOBEC3G suggesting that these inhibitors can impede the mechanisms used by the virus to evade the innate defences of the host cell (Stopak et al. 2003; Sheehy et al. 2003; Mehle et al. 2004; Yu et al. 2003) . Targeting an essential cellular process like the proteosome is anticipated to be cytotoxic and not well tolerated in vivo. Nevertheless, the highly specifi c proteosome inhibitor epoxomicin, which also inhibits HIV-1 maturation, is well tolerated in mice (Meng et al. 1999) . The proteosome inhibitor, PS-341, is approved as a last resort treatment of multiple myelomas and is associated with adverse effects (Kane et al. 2006) . The use of proteosome inhibitors in HIV-1 infected individuals needs to be considered in the context of the potential risk benefi t and the net effect on inhibition of HIV-1 replication as proteosome inhibitors also enhance the early step of the virus life-cycle by preventing degradation of the reverse transcription complex mediated by TRIM5α (Schwartz et al. 1998; Wu et al. 2006; Wei et al. 2005 ). The HIV-1 accessory protein, Vpu is a 16 kDa type 1 integral membrane protein that is indispensable for viral pathogenesis (Li et al. 2005) . Vpu plays two distinctive roles in the viral life-cycle that include down regulating host cell CD4 receptors (Willey et al. 1992) and enhancing viral particle release from the cell surface, the latter associated with its ion channel forming properties (Schubert et al. 1996a) . Vpu is unique to HIV-1/SIVcpz viruses (Binette and Cohen, 2004) . Interestingly the two closely related retroviruses HIV-2 and SIV, which lack Vpu, are less pathogenic (Bour and Strebel, 2003) . The role of Vpu in the viral budding process is coupled to its ion channel forming properties, which is predicted to be a pentameric structure composed of fi ve transmembrane domains (Grice et al. 1997) . The Vpu ion channel is thought to function by altering the electric potential at the plasma membrane or alternatively by overcoming host restriction factors for viral release (Neil et al. 2006 ). The HIV-1 Vpu is a member of viral ion channel proteins called viroporins and is structurally similar to the M2 ion channel protein of infl uenza (Gonzalez and Carrasco, 2003; Hout et al. 2006 ). An interesting feature of Vpu is its role in viral particle release from nondividing cells such as macrophages (Deora and Ratner, 2001) . In this regard the rate of host cell proliferation is a determining factor for Vpu mediated viral particle release (Deora and Ratner, 2001) . Analogues of amiloride (a sodium channel blocker) inhibit Vpu ion channel activity. The amiloride analogues, 5-(N,N-hexamethylene) amiloride (HMA) and 5-(N,N-dimethyl)amiloride (DMA) inhibit Vpu mediated virus budding and viral replication in macrophages (Ewart et al. 2002; Ewart et al. 2004) . The inhibitory effects are observed in the absence of cytotoxicity. Both analogs exhibit strong inhibition of HIV-1 replication as measured by viral p24 levels in culture supernatants (Ewart et al. 2004 ). HMA at 4 µM suppresses viral p24 in culture supernatants to undetectable levels for more than 10 days in culture (Ewart et al. 2004) . While amiloride analogues demonstrate activity in macrophages, they fail to inhibit HIV-1 replication in T-cells (Ewart et al. 2002) . Nevertheless, Vpu ion channel inhibitors have the potential for use in combination therapy, targeting viral reservoirs and drug-resistant variants. An amiloride derivative, BIT225, is currently being pursued for drug development (Biotron Limited, Sydney, NSW, Australia). BIT225 represents a promising antiretroviral therapeutic although the evaluation of its in vivo effi cacy will present a challenge since inhibition of HIV-1 replication appears to be restricted to nonproliferating cells. Recent studies also suggest that rimantadine, an ion channel blocker of infl uenza A viruses, can be a useful lead compound for designing Vpu inhibitors. Rimantadine and amantadine belong to class of polycyclic amines that are active against the M2 ion channel of infl uenza A but not against HIV-1 Vpu (Hout et al. 2006) . Studies indicate that mutating histidine at residue 19 to alanine results in a rimantadine sensitive Vpu ion channel demonstrating the potential of this class of inhibitor as HIV-1 ion channel blockers (Hout et al. 2006) . Vpu is also implicated to interact with certain host cell restriction factors that interfere with viral particle egress. The host cell protein, Vpu-binding protein (UBP), is suggested to be a negative factor for virus assembly (Callahan et al. 1998; Bour and Strebel, 2003) . UBP is a 34-kDa protein that exhibits competitive binding with Vpu and Gag (Callahan et al. 1998; Handley et al. 2001 ). Overexpression of UBP has been reported to significantly suppress viral particle release suggesting that UBP is a negative factor that requires displacement by Gag or Vpu (Callahan et al. 1998) . It is suggested that Vpu mediates its effect on viral egress by facilitating membrane targeting of Gag precursors (Handley et al. 2001; Harila et al. 2006; Neil et al. 2006) . Supporting this notion, Vpu-defective particles appear in internal membrane-bound compartments suggesting a Gag targeting defect (Klimkait et al. 1990 ). Another host cell restriction factor implicated in viral release is an acid-sensitive potassium channel-forming protein, TASK-1, which is down regulated during viral infection (Hsu et al. 2004 ). Due to the structural homology of TASK-1 and HIV-1 Vpu, TASK-1 has been suggested to form hetero-oligomers with Vpu which interferes with both TASK-1 mediated conductance and Vpu ion channel function (Hsu et al. 2004 ). Further delineation of the how these host cell factors interact with Vpu is required in order to design small molecule inhibitors that inhibit viral particle release. In the recent years, major advances have been made in our understanding of how HIV-1 and other retroviruses are released from infected cells. In the early 1990s it was reported that mutations in the p6 late domain inhibit virion particle release (Gottlinger et al. 1991) . Moreover, a PTAP sequence, encompassing amino acids 7 to 10 of p6, is critical for virus particle production (Huang et al. 1995) . The PTAP motif binds specifi cally to the host cell protein, tumor suppressor gene 101 (TSG101), resulting in the recruitment of components of the endosomal sorting complex required for transport-I (ESCRT-I) (VerPlank et al. 2001; Martin-Serrano et al. 2001; Garrus et al. 2001; Demirov et al. 2002) . Deletion of the PTAP motif results in approximately 80% reduction in HIV-1 particle release. Similarly, overexpression and silencing of TSG101 abolish viral egress (Garrus et al. 2001; Demirov et al. 2002; Goila-Gaur et al. 2003) . NMR structure analysis of a 14 amino acid peptide derived from p6 encompassing the PTAP motif complexed with the UEV domain of TSG101 has revealed that the PTAP motif binds to a groove in TSG101. This binding creates two main pockets: the "A-P" pocket through contact of amino acids 7-10 and the "P" pocket through the binding of amino acids 9-10 of the peptide (Pornillos et al. 2002a; Pornillos et al. 2002b ). Interruption of this viral host protein-protein interaction with compounds that bind to this pocket in TSG101 and compete with Gag would potentially inhibit viral particle release and the rate of cell-cell HIV-1 transmission (Bieniasz, 2006) . So far there has been one report that describes the synthesis and selection of small molecules with up to fi ve fold better binding capacity than a peptide that contains the sequence of the wild type PTAP motif in the L domain of HIV-1 (Liu et al. 2006) . In this study, the authors describe an approach previously employed to obtain peptoids in which a key proline residue is substituted with glycine at the N-terminus of the parent Pro-rich sequence in order to improve binding specifi city to Src homology 3 (SH3) domains (Nguyen et al. 2000) . Similarly, the proline rich PTAP domain in p6 was considered a good candidate for the N-substituted glycine residue approach. In this study, the Tsg101 binding affi nity (K d ) of the 9-mer wild-type PTAP containing peptide was 50 µM (Liu et al. 2006) . Binding constants for the highest affi nity peptoidhydrazones (designated 11q and 11p) were 17.5 and 9.8 µM, respectively. The highest affi nity peptoid hydrazone was found to be the n-butyl-containing 11p peptide, with a fi ve-fold increase in binding affi nity compared to the wild-type 9-mer PTAP peptide. The capacity of these peptides to compete with HIV-1 Gag for TSG101 binding and their effect on HIV-1 egress in vitro and in vivo remains to be determined. Although disruption of viral-host cell interactions is a very attractive approach to abolish virion release and infectivity, interfering with the host cell machinery could have major consequences for the host (Bieniasz, 2006) . TSG101 is a multifunctional protein and plays a critical role in cell proliferation as shown by studies conducted in TSG101 defi cient mice (Ruland et al. 2001) . TSG101 is involved in cellular transcription and plays a central role in endosomal sorting of cargo protein that is destined for degradation by the proteasome. TSG101 is recruited to the endosomal sorting pathway by a specifi c interaction with the host cell protein, hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs), which binds to TSG101 through a PSAP motif (Lu et al. 2003) . Disruption of this interaction inhibits delivery of epidermal growth factor receptor (EGFR) to the late endosomes. In recruiting TSG101, it is believed that HIV-1 mimics Hrs in order to enter the endosomal sorting pathway and negotiate its release from the infected cell (Pornillos et al. 2003) . Thus, targeting TSG101 would result in the accumulation of proteins at the plasma membrane and the disruption of protein sorting within the infected cell. Specifi c inhibition of HIV-1 budding by targeting the late domain binding site on TSG101 will require preferential inhibition of p6 binding compared to Hrs. Considerable progress has been made in understanding the late steps of the viral life-cycle leading to the production of infectious viral particles. Many of these processes rely on protein:protein interactions either between viral proteins or viral proteins and host cell factors. The interactions between the host and viral proteins have either a role in facilitating virus replication, as is observed for the viral p6 and Tsg101, or are necessary to overcome negative effects of the host on virus replication, as mediated by Vif. HIV-1 also relies on posttranslational modifi cations mediated by the host cell machinery in order for viral polyproteins to be traffi cked to the appropriate compartment of the cell for viral assembly and budding. Arguably, one of the most effective drugs used to treat HIV-1 infected individuals are the HIV-1 PR inhibitors that block viral maturation. This underscores the effectiveness of targeting the late stage of virus replication. Nevertheless, while some of the strategies described in this review inhibit virus replication in cell culture assays, they require improvements in potency, specifi city and delivery before being viable for use in the clinic. HIV protease inhibitors: peptidomimetic drugs and future perspectives A putative alphahelical structure which overlaps the capsid-p2 boundary in the human immunodefi ciency virus type 1 Gag precursor is crucial for viral particle assembly Vitro Resistance to the Human Immunodeficiency Virus Type 1 Maturation Inhibitor PA-457 (Bevirimat) Mutations of RNA and protein sequences involved in human immunodeficiency virus type 1 packaging result in production of noninfectious virus Glycine-amide is an active metabolite of the antiretroviral tripeptide glycyl-prolyl-glycine-amide No crossresistance or selection of HIV-1 resistant mutants in vitro to the antiretroviral tripeptide glycyl-prolyl-glycine-amide Mutational analysis of Tyr-501 of HIV-1 reverse transcriptase. Effects on ribonuclease H activity and inhibition of this activity by N-acylhydrazones The integrase interactor 1 (INI1) proteins facilitate Tat-mediated human immunodefi ciency virus type 1 transcription Time dependent heterodimer formation leads to inhibition of HIV protease activity Inhibition of HIV protease activity by heterodimer formation Synthetic "interface" peptides alter dimeric assembly of the HIV 1 and 2 proteases Trans-dominant inhibitory human immunodefi ciency virus type 1 protease monomers prevent protease activation and virion maturation Human immunodefi ciency virus type 1 (HIV-1) strains selected for resistance against the HIV-1-specific [2′,5′-bis-O-(tertbutyldimethylsilyl)-3′-spiro-5″-(4″-amino-1″,2″-oxathiole-2″,2″-dioxide)]-beta-D-pentofurano syl (TSAO) nucleoside analogues retain sensitivity to HIV-1-specifi c nonnucleoside inhibitors Molecular tongs containing amino acid mimetic fragments: new inhibitors of wildtype and mutated HIV-1 protease dimerization Late budding domains and host proteins in enveloped virus release Recent advances in the understanding of HIV-1 Vpu accessory protein functions Role of myristylation in HIV-1 Gag assembly The HIV-1 Vpu protein: a multifunctional enhancer of viral particle release Design, synthesis, and evaluation of conformationally constrained tongs, new inhibitors of HIV-1 protease dimerization Dimerization inhibitors of HIV-1 protease based on a bicyclic guanidinium subunit Programmed ribosomal frameshifting in HIV-1 and the SARS-CoV A folding inhibitor of the HIV-1 protease Design of HIV-1-PR inhibitors that do not create resistance: blocking the folding of single monomers Myristoylation-dependent replication and assembly of human immunodefi ciency virus 1 Replication of human immunodefi ciency virus 1 and Moloney murine leukemia virus is inhibited by different heteroatom-containing analogs of myristic acid Incorporation of 12-methoxydodecanoate into the human immunodefi ciency virus 1 gag polyprotein precursor inhibits its proteolytic processing and virus production in a chronically infected human lymphoid cell line Lack of integrase can markedly affect human immunodefi ciency virus type 1 particle production in the presence of an active viral protease Design of dimerization inhibitors of HIV-1 aspartic proteinase: a computer-based combinatorial approach Functional interaction of human immunodefi ciency virus type 1 Vpu and Gag with a novel member of the tetratricopeptide repeat protein family TSAO derivatives, inhibitors of HIV-1 reverse transcriptase dimerization: recent progress Self-assembly in vitro of purifi ed CA-NC proteins from Rous sarcoma virus and human immunodefi ciency virus type 1 A novel genetic algorithm for designing mimetic peptides that interfere with the function of a target molecule Intravirion display of a peptide corresponding to the dimer structure of protease attenuates HIV-1 replication Incorporation of pol into human immunodeficiency virus type 1 Gag virus-like particles occurs independently of the upstream Gag domain in Gag-pol Stability and activity of human immunodefi ciency virus protease: comparison of the natural dimer with a homologous, singlechain tethered dimer Characterization of human immunodefi ciency virus type-1 (HIV-1) particles that express protease-reverse transcriptase fusion proteins A domain directly C-terminal to the major homology region of human immunodefi ciency t-ype 1 capsid protein plays a crucial role in directing both virus assembly and incorporation of Gag-Pol Coding sequences upstream of the human immunodefi ciency virus type 1 reverse transcriptase domain in Gag-Pol are not essential for incorporation of the Pr160(gag-pol) into virus particles HIV drug resistance Drug Target Insights Patented HIV-1 integrase inhibitors Inhibition of HIV-1 replication by a peptide dimerization inhibitor of HIV-1 protease The role of non-nucleoside reverse transcriptase inhibitors (NNRTIs) in the therapy of HIV-1 infection Chemical crosslinking of the subunits of HIV-1 reverse transcriptase Overexpression of the N-terminal domain of TSG101 inhibits HIV-1 budding by blocking late domain function Viral protein U (Vpu)-mediated enhancement of human immunodefi ciency virus type 1 particle release depends on the rate of cellular proliferation Insight into the mechanism of a peptide inhibitor of HIV reverse transcriptase dimerization Proline residues in human immunodefi ciency virus type 1 p6(Gag) exert a cell type-dependent effect on viral replication and virion incorporation of Pol proteins Substrate specifi city of Saccharomyces cerevisiae myristoyl-CoA: protein N-myristoyltransferase. Analysis of fatty acid analogs containing carbonyl groups, nitrogen heteroatoms, and nitrogen heterocycles in an in vitro enzyme assay and subsequent identifi cation of inhibitors of human immunodefi ciency virus I replication Independent segregation of human immunodefi ciency virus type 1 Gag protein complexes and lipid rafts Inhibition of human immunodefi ciency virus type 1 reverse transcriptase dimerization using synthetic peptides derived from the connection domain Functional domains of the capsid protein of human immunodefi ciency virus type 1 Decreasing the frameshift effi ciency translates into an equivalent reduction of the replication of the human immunodefi ciency virus type 1 Thyroxine-derivatives of lipopeptides: bifunctional dimerization inhibitors of human immunodefi ciency virus-1 protease Multiple effects of mutations in human immunodefi ciency virus type 1 integrase on viral replication Amiloride derivatives block ion channel activity and enhancement of virus-like particle budding caused by HIV-1 protein Vpu Potential new anti-human immunodefi ciency virus type 1 compounds depress virus replication in cultured human macrophages A large deletion in the matrix domain of the human immunodefi ciency virus gag gene redirects virus particle assembly from the plasma membrane to the endoplasmic reticulum Inhibition of HIV-1 protease by a subunit of didemnaketal A Potent Nonnucleoside Reverse Transcriptase Inhibitors Target HIV-1 Gag-Pol A systematic evaluation of the inhibition of HIV-1 protease by its C-and N-terminal peptides HIV-1 gag proteins: diverse functions in the virus life cycle Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid Structure of the carboxyl-terminal dimerization domain of the HIV-1 capsid protein. Science Assembly properties of the human immunodefi ciency virus type 1 CA protein Tsg101 and the vacuolar protein sorting pathway are essential for HIV-1 budding Structure of the amino-terminal core domain of the HIV-1 capsid protein Defects in human immunodefi ciency virus budding and endosomal sorting induced by TSG101 overexpression Effect of mutations affecting the p6 gag protein on human immunodefi ciency virus particle release Role of capsid precursor processing and myristoylation in morphogenesis and infectivity of human immunodefi ciency virus type 1 Ion channels formed by HIV-1 Vpu: a modelling and simulation study Safety and effi cacy of the HIV-1 integrase inhibitor raltegravir (MK-0518) in treatment-experienced patients with multidrug-resistant virus: a phase II randomised controlled trial Comparative analysis of the sequences and structures of HIV-1 and HIV-2 proteases Association of Vpu-binding protein with microtubules and Vpu-dependent redistribution of HIV-1 Gag protein Vpu and Tsg101 regulate intracellular targeting of the human immunodefi ciency virus type 1 core protein precursor Pr55gag Interaction between human immunodefi ciency virus type 1 reverse transcriptase and integrase proteins The packaging and maturation of the HIV-1 Pol proteins HIV-1 reverse transcriptase structure with RNase H inhibitor dihydroxy benzoyl naphthyl hydrazone bound at a novel site Tripeptide interference with human immunodefi ciency virus type 1 morphogenesis Human immunodefi ciency virus type 1 assembly and lipid rafts: Pr55(gag) associates with membrane domains that are largely resistant to Brij98 but sensitive to Triton X-100 Inhibition of HIV-1 infection in cells expressing an artifi cial complementary peptide A single amino acid substitution within the transmembrane domain of the human immunodefi ciency virus type 1 Vpu protein renders simian-human immunodefi ciency virus (SHIV(KU-1bMC33)) susceptible to rimantadine Mutual functional destruction of HIV-1 Vpu and host TASK-1 channel p6Gag is required for particle production from full-length human immunodefi ciency virus type 1 molecular clones expressing protease Importance of ribosomal frameshifting for human immunodefi ciency virus type 1 particle assembly and replication Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region Characterization of ribosomal frameshifting in HIV-1 gag-pol expression Two effi cient ribosomal frameshifting events are required for synthesis of mouse mammary tumor virus gag-related polyproteins Expression of the Rous sarcoma virus pol gene by ribosomal frameshifting Crystal structure of human immunodefi ciency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA Intracellular expression of human immunodefi ciency virus type 1 (HIV-1) protease variants inhibits replication of wild-type and protease inhibitor-resistant HIV-1 strains in human T-cell lines Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5 United States Food and Drug Administration approval summary: bortezomib for the treatment of progressive multiple myeloma after one prior therapy The activity of the protease of human immunodefi ciency virus type 1 is initiated at the membrane of infected cells before the release of viral proteins and is required for release to occur with maximum effi ciency Partial inhibition of the human immunodefi ciency virus type 1 protease results in aberrant virus assembly and the formation of noninfectious particles Overexpression of the HIV-1 gag-pol polyprotein results in intracellular activation of HIV-1 protease and inhibition of assembly and budding of virus-like particles The human immunodefi ciency virus type 1-specifi c protein vpu is required for effi cient virus maturation and release Active human immunodefi ciency virus protease is required for viral infectivity Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor The sequences of and distance between two cis-acting signals determine the efficiency of ribosomal frameshifting in human immunodefi ciency virus type 1 and human T-cell leukemia virus type II in vivo Human immunodefi ciency virus proteinase dimer as component of the viral polyprotein prevents particle assembly and viral infectivity 4-oxatetradecanoic acid is fungicidal for Cryptococcus neoformans and inhibits replication of human immunodefi ciency virus I Identifi cation of novel interactions in HIV-1 capsid protein assembly by high-resolution mass spectrometry Interactions between HIV-1 nucleocapsid protein and viral DNA may have important functions in the viral life cycle PA-457: a potent HIV inhibitor that disrupts core condensation by targeting a late step in Gag processing Roles of HIV-1 auxiliary proteins in viral pathogenesis and host-pathogen interactions Image reconstructions of helical assemblies of the HIV-1 CA protein Characterization of human immunodeficiency virus type 1 Pr160 gag-pol mutants with truncations downstream of the protease domain Myristoylation as a target for inhibiting HIV assembly: unsaturated fatty acids block viral budding Hydrazone-and hydrazidecontaining N-substituted glycines as peptoid surrogates for expedited library synthesis: Application to the preparation of Tsg101-directed HIV-1 budding antagonists Autoprocessing of HIV-1 protease is tightly coupled to protein folding TSG101 interaction with HRS mediates endosomal traffi cking and receptor down-regulation Designing HIV integrase inhibitors--shooting the last arrow Role of the major homology region of human immunodefi ciency virus type 1 in virion morphogenesis Drug Target Insights A novel antiretroviral class (fusion inhibitors) in the management of HIV infection. Present features and future perspectives of enfuvirtide (T-20) Peptide inhibitors of HIV-1 integrase dissociate the enzyme oligomers HIV-1 and Ebola virus encode small peptide motifs that recruit Tsg101 to sites of particle assembly to facilitate egress Engineering human immunodefi ciency virus 1 protease heterodimers as macromolecular inhibitors of viral maturation Vif overcomes the innate antiviral activity of APOBEC3G by promoting its degradation in the ubiquitin-proteasome pathway Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinfl ammatory activity New constrained "molecular tongs" designed to dissociate HIV-1 protease dimer Crystal structure of dimeric HIV-1 capsid protein Complete inhibition of human immunodefi ciency virus Gag myristoylation is necessary for inhibition of particle budding The thumb domain of the p51-subunit is essential for activation of HIV reverse transcriptase A new potent HIV-1 reverse transcriptase inhibitor. A synthetic peptide derived from the interface subunit domains Targeting the assembly of the human immunodefi ciency virus type I HIV-1 Vpu promotes release and prevents endocytosis of nascent retrovirus particles from the plasma membrane Improving SH3 domain ligand selectivity using a non-natural scaffold Inhibition of infectious human immunodeficiency virus type 1 particle formation by Gag protein-derived peptides Phosphatidylinositol (4,5) bisphosphate regulates HIV-1 Gag targeting to the plasma membrane Binding of human immunodefi ciency virus type 1 Gag to membrane: role of the matrix amino terminus Role of the Gag matrix domain in targeting human immunodefi ciency virus type 1 assembly Retroviruses have differing requirements for proteasome function in the budding process Declining morbidity and mortality among patients with advanced human immunodefi ciency virus infection. HIV outpatient study investigators In vitro antiviral activities of myristic acid analogs against human immunodefi ciency and hepatitis B viruses Overexpression of the gag-pol precursor from human immunodefi ciency virus type 1 proviral genomes results in effi cient proteolytic processing in the absence of virion production The nonmyristylated Pr160gag-pol polyprotein of human immunodefi ciency virus type 1 interacts with Pr55gag and is incorporated into virus like particles Genetic selection for dissociative inhibitors of designated protein-protein interactions Deletion of sequences upstream of the proteinase improves the proteolytic processing of human immunodefi ciency virus type 1 Processing sites in the human immunodefi ciency virus type Gag-Pro-Pol precursor are cleaved by the viral protease at different rates The p2 domain of human immunodefi ciency virus type 1 Gag regulates sequential proteolytic processing and is required to produce fully infectious virions The regulation of sequential processing of HIV-1 Gag by the viral protease An update and review of antiretroviral therapy Antiviral phospholipids. Anti-HIV drugs conjugated to the glycerobackbone of phospholipids Structure of the Tsg101 UEV domain in complex with the PTAP motif of the HIV-1 p6 protein Structure and functional interactions of the Tsg101 UEV domain HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein Didemnaketal A and B, HIV-1 Protease Inhibitors from the Accidian Didemnum sp Triterpenes as potential dimerization inhibitors of HIV-1 protease Extensive regions of pol are required for effi cient human immunodefi ciency virus polyprotein processing and particle maturation Synthesis and processing of viral RNA The role of Gag in human immunodefi ciency virus type 1 virion morphogenesis and early steps of the viral life cycle Inhibition of multiple phases of human immunodefi ciency virus type 1 replication by a dithiane compound that attacks the conserved zinc fingers of retroviral nucleocapsid proteins Inhibitors of HIV nucleocapsid protein zinc fingers as candidates for the treatment of Identifi cation of a putative binding site for [2′,5′-bis-O-(tert-butyldimethylsilyl)-beta-D-ribofuranosyl]-3′-spiro-5″-(4″-amino-1″,2″-oxathiole-2″,2″-dioxide)thymine (TSAO) derivatives at the p51-p66 interface of HIV-1 reverse transcriptase Infl uence of human immunodefi ciency virus nucleocapsid protein on synthesis and strand transfer by the reverse transcriptase in vitro Macromolecular inhibitors of HIV-1 protease. Characterization of designed heterodimers p53 accumulation, defective cell proliferation, and early embryonic lethality in mice lacking tsg101 Structural basis for targeting HIV-1 Gag proteins to the plasma membrane for virus assembly 3-O-(3′,3′-dimethysuccinyl) betulinic acid inhibits maturation of the human immunodefi ciency virus type 1 Gag precursor assembled in vitro In vivo antiviral activity of novel human immunodefi ciency virus type 1 nucleocapsid p7 zinc fi nger inhibitors in a transgenic murine model Preclinical evaluation of a zinc fi nger inhibitor targeting lentivirus nucleocapsid protein in SIV-infected monkeys The inhibition of human immunodefi ciency virus proteases by 'interface peptides Lipopeptides as dimerization inhibitors of HIV-1 protease HIV-1 reproduction is inhibited by peptides derived from the N-and C-termini of HIV-1 protease Identifi cation of an ion channel activity of the Vpu transmembrane domain and its involvement in the regulation of virus release from HIV-1-infected cells Proteasome inhibition interferes with gag polyprotein processing, release, and maturation of HIV-1 and HIV-2 Antiviral activity of the proteasome on incoming human immunodefi ciency virus type 1 Antiviral agent based on the non-structural protein targeting the maturation process of HIV-1: expression and susceptibility of chimeric Vpr as a substrate for cleavage by HIV-1 protease The antiretroviral enzyme APOBEC3G is degraded by the proteasome in response to HIV-1 Vif Maintenance of the Gag/Gag-Pol ratio is important for human immunodefi ciency virus type 1 RNA dimerization and viral infectivity Proteolytic processing of the p2/nucleocapsid cleavage site is critical for human immunodefi ciency virus type 1 RNA dimer maturation Antiretroviral compounds: mechanisms underlying failure of HAART to eradicate HIV-1 Genetic analysis of the human immunodefi ciency virus type 1 integrase protein Interactions of HIV-1 Gag with assembly cofactors Proteolytic processing of an HIV-1 pol polyprotein precursor: insights into the mechanism of reverse transcriptase p66/p51 heterodimer formation Human immunodefi ciency virus type 1 reverse transcriptase dimer destabilization by 1 Structure-activity relationships of [2′,5′-bis-O-(tert-butyldimethylsilyl)-beta-D-ribofuranosyl]-3′-spiro-5″-(4″-amino-1″,2″-oxathiole-2″,2″-dioxide)thymine derivatives as inhibitors of HIV-1 reverse transcriptase dimerization Modulation of the oligomeric structures of HIV-1 retroviral enzymes by synthetic peptides and small molecules Requirements for incorporation of Pr160gag-pol from human immunodefi ciency virus type 1 into virus-like particles Design and synthesis of new inhibitors of HIV-1 protease dimerization with conformationally constrained templates HIV-1 replication in cell lines harboring INI1/hSNF5 mutations Characterization of deletion mutations in the capsid region of human immunodefi ciency virus type 1 that affect particle formation and Gag-Pol precursor incorporation Dimerization of human immunodefi ciency virus type 1 reverse transcriptase as an antiviral target Researchers buoyed by novel HIV drugs: will expand drug arsenal against resistant virus A peptide inhibitor of HIV-1 assembly in vitro Drug Target Insights HIV-1 Vif blocks the antiviral activity of APOBEC3G by impairing both its translation and intracellular stability Synthesis, assembly, and processing of viral proteins Role of residues in the tryptophan repeat motif for HIV-1 reverse transcriptase dimerization Analysis of mutations and suppressors affecting interactions between the subunits of the HIV type 1 reverse transcriptase Efavirenz enhances the proteolytic processing of an HIV-1 pol polyprotein precursor and reverse transcriptase homodimer formation Nonnucleoside reverse transcriptase inhibitors are chemical enhancers of dimerization of the HIV type 1 reverse transcriptase Relationship between enzyme activity and dimeric structure of recombinant HIV-1 reverse transcriptase Formation of stable and functional HIV-1 nucleoprotein complexes in vitro Antiviral inhibition of the HIV-1 capsid protein Entropic switch regulates myristate exposure in the HIV-1 matrix protein Structure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein Reverse transcriptase and the generation of retroviral DNA The HIV-1 capsid protein C-terminal domain in complex with a virus assembly inhibitor The structural stability of the HIV-1 protease Inhibitors of human immunodefi ciency virus type 1 zinc fi ngers prevent normal processing of gag precursors and result in the release of noninfectious virus particles A modular approach to HIV-1 proteinase inhibitor design Restricting the fl exibility of crosslinked, interfacial peptide inhibitors of HIV-1 protease Effects of efavirenz binding on the subunit equilibria of HIV-1 reverse transcriptase Biochemical and immunological analysis of human immunodefi ciency virus gag gene products p17 and p24 Tsg101, a homologue of ubiquitin-conjugating (E2) enzymes, binds the L domain in HIV type 1 Pr55(Gag) Nucleoside and nucleotide inhibitors of HIV-1 replication Proteolytic processing and particle maturation Functional surfaces of the human immunodefi ciency virus type 1 capsid protein Mutations that abrogate human immunodefi ciency virus type 1 reverse transcriptase dimerization affect maturation of the reverse transcriptase heterodimer Inhibition of lysosome and proteasome function enhances human immunodeficiency virus type 1 infection Sequential steps in human immunodeficiency virus particle maturation revealed by alterations of individual Gag polyprotein cleavage sites Human immunodefi ciency virus type 1 Vpu protein induces rapid degradation of CD4 Conserved folding in retroviral proteases: crystal structure of a synthetic HIV-1 protease Structures of the HIV-1 capsid protein dimerization domain at 2.6 A resolution Proteasome inhibitors uncouple rhesus TRIM5alpha restriction of HIV-1 reverse transcription and infection Functional RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein Update on HAART in HIV Antiretroviral treatment for adult HIV infection in 2002: updated recommendations of the International AIDS Society-USA Panel Mutations in the primer grip of human immunodefi ciency virus type 1 reverse transcriptase impair proviral DNA synthesis and virion maturation Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Inhibition of HIV-1 virion production by a transdominant mutant of integrase interactor 1 Specifi city of interaction of INI1/hSNF5 with retroviral integrases and its functional signifi cance Dissociative inhibition of dimeric enzymes. Kinetic characterization of the inhibition of HIV-1 protease by its COOH-terminal tetrapeptide Interfacial peptide inhibitors of HIV-1 integrase activity and dimerization The sequence of the CA-SP1 junction accounts for the differential sensitivity of HIV-1 and SIV to the small molecule maturation inhibitor 3-O-{3′,3′-dimethylsucci-nyl}-betulinic acid Drug Target Insights HIV-1 Resistance to the Small Molecule Maturation Inhibitor 3-O-{3′,3′-dimethylsuccinyl}-betulinic acid is conferred by a variety of single amino acid substitutions at the CA-SP1 cleavage site in Gag Inhibition of HIV-1 maturation via drug association with the viral Gag protein in immature HIV-1 particles Targeting the dimerization interface for irreversible inhibition of HIV-1 protease