key: cord-0279626-l2xb3wgn authors: Hollstein, M. M.; Muensterkoetter, L.; Schoen, M. P.; Bergmann, A.; Husar, T. M.; Abratis, A.; Eidizadeh, A.; Schaffrinski, M.; Zachmann, K.; Schmitz, A.; Holsapple, J. S.; Stanisz-Bogeski, H.; Schanz, J.; Gross, U.; Leha, A.; Zautner, A. E.; Schnelle, M.; Erpenbeck, L. title: Interdependencies between cellular and humoral immune responses in heterologous and homologous SARS-CoV-2 vaccination date: 2021-12-14 journal: nan DOI: 10.1101/2021.12.13.21267729 sha: 229a26ab04ea97f2ccd0e00b39a36057222c48bd doc_id: 279626 cord_uid: l2xb3wgn Background: Homologous and heterologous SARS-CoV-2-vaccinations yield different spike protein-directed humoral and cellular immune responses. However, their interdependencies remain elusive. Methods: COV-ADAPT is a prospective, observational cohort study of 417 healthcare workers who received homologous vaccination with Astra (ChAdOx1-S; AstraZeneca) or BNT (BNT162b2; Biontech/Pfizer) or heterologous vaccination with Astra/BNT. We assessed the humoral (anti-spike-RBD-IgG, neutralizing antibodies, antibody avidity) and cellular (spike-induced T cell interferon-y release) immune response in blood samples up to 2 weeks before (T1) and 2 to 12 weeks following secondary immunization (T2). Findings: Initial vaccination with Astra resulted in lower anti-spike-RBD-IgG responses compared to BNT (70+/-114 vs. 226+/-279 BAU/ml, p<0.01) at T1, whereas T cell activation did not differ significantly. Booster vaccination with BNT proved superior to Astra at T2 (anti-spike-RBD-IgG: Astra/BNT 2387+/-1627 and BNT/BNT 3202+/-2184 vs. Astra/Astra 413+/-461 BAU/ml, both p<0.001; spike-induced T cell interferon-y; release: Astra/BNT 5069+/-6733 and BNT/BNT 4880+/-7570 vs. Astra/Astra 1152+/-2243 mIU/ml, both p<0.001). No significant differences were detected between BNT-boostered groups at T2. For Astra, we observed no booster effect on T cell activation. We found associations between anti-spike-RBD-IgG levels (Astra/BNT and BNT/BNT) and T cell responses (Astra/Astra and Astra/BNT) from T1 to T2. There were also links between levels of anti-spike-RBD-IgG and T cell at both time points (all groups combined). All regimes yielded neutralizing antibodies and increased antibody avidity at T2. Interpretation: Interdependencies between humoral and cellular immune responses differ between common SARS-CoV-2 vaccination regimes. T cell activation is unlikely to compensate for poor humoral responses. Funding: Deutsche Forschungsgemeinschaft (DFG), ER723/3-1 We searched Pubmed for papers published between 01/01/2019 and 14/05/2021 with the search terms "covid-19" combined with "vaccination" and "heterologous", excluding "BCG". Of the 41 papers found, none addressed the combination of ChAdOx1-S by AstraZeneca (Astra) and BNT162b2 by Biontech/Pfizer (BNT). After our study was initiated, the CombiVacS trial reported a significant booster effect when BNT was given after initial vaccination with Astra. 1 The investigators of the CoCo trial subsequently published data on heterologous immunization in comparison to homologous Astra in a small population (n=87), with the heterologous immunization scheme showing a superior humoral and cellular immune response. 2 Further studies investigated heterologous vaccinations with Astra and BNT as well as homologous Astra and BNT regimes and also found superior humoral and cellular immune responses in the heterologous regimes compared to homologous Astra, and comparable or slightly superior immune responses when compared to homologous BNT vaccination. [3] [4] [5] [6] The body of research covering the effects of heterologous immunization regimes has recently been aggregated in a systematic review. 7 To our knowledge, this is the first study that evaluates the interdependencies of cellular and humoral immune responses following heterologous vaccination with Astra/BNT in a large group of individuals. Our data show strong correlations between humoral and cellular immune responses with the prime-boost combination Astra/BNT. The findings suggest that individuals with a robust initial response developed strong humoral and cellular immune responses after booster immunization. Our study and the available data suggest that due to its superior capacity to elicit a humoral and cellular immune response, mRNA-based vaccines such as BNT should be chosen for booster vaccination rather than Astra. This seems to be particularly important in individuals whose immune response was poor after initial vaccination with Astra. March 2020 and has since resulted in more than five million casualties worldwide. [8] [9] [10] [11] The virus enters macrophages, type II pneumocytes, pericytes and muscle cells by utilizing the angiotensin-converting enzyme 2. 12 As a reaction, innate and acquired immune responses are mounted, including the production of antibodies and specific T cells. 13, 14 Symptoms develop approximately five to six days after infection. 15, 16 During SARS-CoV-2 infection, some individuals appear to be at higher risk for severe or even life-threatening disease. Higher age, male sex and severe preexisting health conditions are the most important determinants for such outcomes. 17 In some patients, long-lasting symptoms occur, sometimes even after initially mild or asymptomatic disease in young and previously healthy individuals. Recently, the WHO defined "Long-COVID" as a disease course with symptoms such as fatigue, shortness of breath and cognitive dysfunction that continue for more than two months after a SARS-CoV-2 infection. 18 Thus, it is imperative to protect the population from infection. While SARS-CoV-2 expresses four major structural proteins (spike (S), membrane (M), envelope (E) and nucleocapsid (N) proteins), vaccine production has focused on the spike protein because of its immunogenicity and its importance for the induction of neutralising antibodies. [19] [20] [21] Subjects inoculated with the current EMA-authorized vaccines including ChAdOx1-S (Vaxzevria, AstraZeneca, Oxford, UK), Janssen United States) develop significant levels of antibodies against the spike protein. [22] [23] [24] [25] [26] Vaccines that immunize against SARS-CoV-2 epitopes provide efficient protection from the virus. Overall, levels of anti-spike RBD-IgG and neutralizing antibodies (which correlate strongly) appear to be good measures of vaccine efficacy. [27] [28] [29] Recent publications report a vaccine efficiency of 80% against asymptomatic infections with the Alpha variant (B.1.1.7), with anti-spike-RBD-IgG titers of 506 BAU/ml or 90% efficacy for titers above 775 BAU/ml. 29, 30 Similar studies for the Delta (B.1.617.2) or All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in 31 It must be assumed that higher antibody titers are necessary to prevent infections. We are still in the process of determining optimal dosing intervals and combinations of the currently available vaccines. The ongoing debate about waning IgG titers only months after vaccination in the face of new SARS-CoV-2 variants underscores the need for a better understanding of different vaccine regimes. When reports began to surface on cerebral venous thrombosis following Astra (ChAdOx1-S) vaccinations, particularly in younger individuals, many European governments, including Germany, restricted its use to individuals over a certain age. In Germany, this age was set at 60 years and older. This left some individuals who had been initially vaccinated with Astra insufficiently protected. As a consequence, a heterologous Astra/BNT (BNT162b2 immunization was proposed, even though at that time there was a lack of data on immunogenicity and safety. [32] [33] [34] [35] In the meantime, however, considerably more data have become available and these indicate that heterologous immunization with Astra/BNT is safe and at least equally effective to the homologous BNT/BNT regimen. [1] [2] [3] [4] [5] [6] Nevertheless, particularly cellular immune responses as well as individual titer developments after vaccination have not been assessed in larger cohorts. Due to the lack of comprehensive data on SARS-CoV-2specific humoral (e.g. IgG titers against spike protein) and cellular immune responses (e.g. spike protein-directed T cell responses) on the individual level, little is known about the relationship between these two branches of the adaptive immune system. There are associations between the level of anti-SARS-CoV-2 spike antibodies and/or the level of neutralising antibodies (nABs) and protection from symptomatic and/or asymptomatic COVID-19 following vaccination. [27] [28] [29] However, nABs do not seem to be the sole mechanistic correlate of protection; T cells may also contribute considerably to protective immunity against SARS-CoV-2. 36 Little is known about the relative importance of antibodies and T cells in preventing future SARS-CoV-2 infections. A first step towards clarification of this question is to assess T cells and antibodies following infection or vaccination at the individual level. All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in Recent research has shown declining antibody titers and waning vaccine efficacy over time. 26, 37, 38 For homologous BNT, vaccine efficacy declined from 96% shortly after completion of the vaccination regime to 84% four months later. 39 A decreasing vaccine efficacy will arguably be caused by a significant change in the immune reaction, but this is still not fully understood. Data on the correlation between humoral and cellular vaccination responses will over time provide guidance for establishing the ideal timing for booster vaccines. The goal of this study was thus to determine and correlate IgG and T cell responses against the spike protein following heterologous Astra/BNT vaccination and compare them to individuals vaccinated with the Astra/Astra or BNT/BNT regimens, respectively. The prospective, observational COV-ADAPT cohort study was conducted at the University Medical Center Göttingen, Germany (UMG). Employees and affiliates of the UMG between the ages of 18 and 75 years who received routine first (prime) and second (booster) vaccination against COVID-19 at the UMG vaccination center were eligible for inclusion in the study unless they were currently afflicted with COVID-19 or were in domestic quarantine. The COV-ADAPT study was approved by the ethics committee of the University Medical Center Göttingen (21/5/21). Study design and study implementation were performed in accordance with the guidelines of Good Clinical Practice (ICH 1996) perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in After written informed consent was obtained, a questionnaire was handed out and blood samples were collected. We assessed vaccination regime, age, gender, previous COVID-19 infection or contact, medications, comorbid diseases and whether any family members were attending childcare facilities. Study subjects were only labelled "post COVID-infection", if they had had a SARS-CoV-2 infection proven by PCR testing. At the UMG, positive SARS-CoV-2 antigen-tests are routinely verified by PCR testing and thus no study subject had a positive antigen test without subsequent confirmation by PCR testing. All other indications of a prior SARS-CoV-2 infection were summarized as "COVID contact". We registered and excluded all cases with immunosuppressive medication as well as those whose medication frequently has immunologically relevant side effects. The study was designed to exclude subjects with comorbidity that severely affect the immune system such as leukemia or innate immune deficiencies; however, no study participant reported such comorbid diseases. We did not exclude subjects whose concomitant diseases were expected to have no or at most moderate influence on the outcome of vaccination such as diabetes mellitus, cardiovascular disease, asthma or allergies. Blood samples were collected at the UMG up to two weeks before (T1) and two weeks to three months following booster immunization (T2). The following analyses were performed: 1) IgG antibodies directed against the receptor binding domain of the spike protein perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in The copyright holder for this this version posted December 14, 2021. ; https://doi.org/10.1101/2021.12.13.21267729 doi: medRxiv preprint 5) The cellular immune response using the SARS-CoV-2-spike-specific-IFN-γrelease assay (IGRA) (Euroimmun) on the DSX Automated ELISA System (Thermo Labsystems). Statistical analyses were performed by the Scientific Core Facility for Medical Biometry and Statistical Bioinformatics (MBSB) of the UMG using the statistics software R. The significance levels were set to alpha=5% for all statistical tests. For detailed information on methods and materials see supplementary material. From May 14 th to July 14 th , 2021, we recruited 417 participants between the ages of 18 and 65 years for a first blood sampling (T1) up to two weeks prior to their receiving a routine COVID-19 booster vaccination at the UMG vaccination center (see schematic in Figure 1 ). The average age was 35 years, with 309 female and 106 male participants. Two participants did not disclose their gender. From the 417 participants, 19 were analyzed separately for one of the following reasons: (i) a history of previously proven COVID-19 (n=8); (ii) detectable positive or border-line anti-NCP IgG antibody titers despite anamnestic negative COVID-history (n=7); (iii) insufficient information on the initial vaccine (n=1); (iv) immunosuppressive drug intake (n=3). In the latter, the immunosuppressive drugs included adalimumab (n=1), ropeginterferon alfa-2b (n=1) and apremilast (n=1). The rest of the study participants had received either Astra as their prime vaccination (n=326 subjects) or BNT (n=72). We asked the participants for a second blood sample to be taken 2 to 3 months after booster vaccination (T2). Of 417 invited subjects, 36 did not respond. Two of these individuals had had COVID-19 and chose not to receive a booster within the study period (n=2). Others did not show up for sampling but did provide us with information on their booster vaccination (n=20). The rest (n=14) did not respond at all, even after All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in The copyright holder for this this version posted December 14, 2021. ; https://doi.org/10.1101/2021.12.13.21267729 doi: medRxiv preprint we tried to contact them four times. The lack of information on their booster vaccinations was noted and they were excluded from further analysis. We also excluded the 19 aforementioned participants with immune system modifications or proven contact to SARS-CoV-2 from the analysis of the second blood samples, but samples from these individuals were analyzed separately. The remaining 384 study participants were allocated to groups according to their vaccination regimes: 27 participants had received homologous Astra/Astra, 287 had received Astra/BNT and 68 were vaccinated using homologous BNT/BNT. Two subjects had received an Astra/Moderna (mRNA-1273, Spikevax, Moderna, Cambridge, United States) combination. This group was too small in size and hence was excluded from further analysis. The three groups (Astra/Astra, Astra/BNT, and BNT/BNT) differed somewhat in terms of participant characteristics: Based on German vaccination policies, subjects with homologous Astra vaccinations were older on average than subjects with a BNT booster (see Table 1 ). For further analyses, we accounted for group inhomogeneity by controlling for age and sex. Strong primary immune response with mRNA (BNT) and high anti-spike-RBD-IgG titers after booster with BNT, independent of prime vaccination We analyzed titers of IgG against the spike-RBD at T1 ( Figure 1A ) and found that individuals who had received BNT as their prime vaccination had achieved significantly higher titers, with an average of 226±279 BAU/ml (mean ± standard deviation (SD)), as compared to individuals whose prime vaccination was with Astra (70±114 BAU/ml). Samples at T2 showed that booster vaccinations significantly increased the levels of anti-spike-RBD-IgG for all included vaccine combinations ( Figure 2A ). However, we observed a superior anti-spike-RBD-IgG response for subjects with a BNT booster regardless of the initial vaccine, as compared to homologous Astra vaccination ( p=0.039). Subjects with higher titers after prime vaccination achieved higher titers after All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in We further analyzed whether age (Supplementary Figure 1A) , time between booster and T2 (Supplementary Figure 1C) , or sex (Supplementary Figure 2) impacted humoral vaccination outcomes. It was found that anti-spike-RBD-IgG titers showed a trend towards lower titers at higher ages only in the groups with a BNT booster (Supplementary Figure 1A) , but not in the group that had received homologous Astra vaccine. Sex did not have a significant influence (Supplementary Figure 2) . Furthermore, the magnitude of anti-spike-RBD-IgG titers changed significantly between the booster vaccination and T2 (Supplementary Figure 1B) . While the antibody response tended to increase with elapsed time in the Astra/Astra group (albeit not significantly), titers declined in both groups that had received the BNT booster (significant in the Astra/BNT but not in the BNT/BNT group). Robust spike-directed T cell response after mRNA (BNT) booster but not after homologous Astra vaccination Next, we measured the spike-directed IFN-γ T cell response in our study groups. There was no significant difference between persons whose prime vaccination was with either Astra or BNT at T1, with averages of 707±1631 mIU/ml and 1277±2514 mIU/ml ( Figure 3A, dots) . In contrast to anti-spike-RBD-IgG titers, the distribution was not strikingly different as medians partially overlapped ( Figure 3B ). Remarkably, there was also no statistically significant booster effect between T1 and T2 in subjects homologously vaccinated with Astra. In contrast, boostering with BNT yielded a significantly increased IFN-γ T cell response at T2, regardless of the prime vaccination ( Figure 3A , blue triangles). Similar to the findings with the anti-spike-RBD-IgG, there was no significant difference in the T cell response between the Astra/BNT and the BNT/BNT group. Regression analysis between T cell responses at T1 and T2 ( Figure 3C perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in In all three groups (Astra/Astra, Astra/BNT and BNT/BNT), there were measurable titers of neutralizing antibodies already at T1. We found a significant increase in the NI from T1 to T2, most pronounced in both the Astra/Astra and the Astra/BNT groups, and more moderate in the BNT/BNT group. The moderate increase in the BNT/BNT group can be attributed to the already high level of neutralization indices at T1 ( Figure 6 ). Interestingly, in both BNT-boostered groups, a small number of participants (31/276 for the Astra/BNT group and 6/60 for the BNT/BNT group) presented with no neutralizing antibodies at T2, even though the majority of these participants had had measurable neutralizing antibodies at T1. Only three subjects showed neither neutralizing antibodies at T1 nor at T2, all of whom had received homologous BNT. Of note, the lack of neutralizing antibodies did not correspond to a lack of anti-spike-RBD-IgG, as these participants did have above average anti-spike-RBD-IgG titers. Avidity, a measure for the binding strength of a multivalent bond between antigen and antibody, is often used as a parameter of the quality of an antibody response. In our study, persons who were prime vaccinated with Astra reached a significantly higher avidity index at T1 when compared to BNT ( Figure 6B ). All groups showed a significant increase in avidity at T2. Interestingly, the BNT/BNT group also reached the lowest avidity at T2, which was significantly lower than for both groups with prime vaccination with Astra. We thus found that the ratio between antibody titers and antibody avidity is different between the vaccination schemes. The reason for this could either be that (i) Astra yields fewer but more avid antibodies or (ii) our avidity assay is insufficiently quantitative. COV-ADAPT is an observational cohort study providing real-world data on heterologous vaccination with Astra/BNT compared to homologous Astra/Astra and BNT/BNT-vaccinations in a large cohort of 417 health care workers. As the focus of this study was the evolution of the immune response after prime and booster vaccination, baseline values of participants before both vaccines were not included. All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in We correlated immune responses on an individual level after prime vaccination with the responses after booster to evaluate the predictability of the quality of the immune response. We were able to demonstrate that humoral and cellular immune responses correlate with one another, suggesting that a poor humoral immune response is unlikely to be ameliorated by a strong cellular response. In our study, we found a superior effectiveness in regard to immune stimulation for the mRNA-based vaccines (i.e. BNT), either in a homologous BNT/BNT vaccine regime or as a heterologous combination with Astra (Astra/BNT). This superior effectiveness was observed both in terms of the humoral immune response (anti-spike-RBD-IgG titers) as well as the cellular component, i.e. the spike-directed IFN-γ T cell responses. Our findings corroborate with previous studies which also demonstrated that the IgG antibody response against the spike protein in the heterologous Astra/BNT regime is comparable to the homologous BNT/BNT and superior to the homologous Astra/Astra regime. [2] [3] [4] [5] [6] Furthermore, these studies showed that heterologous immunization with Astra/BNT yields a tolerable and manageable reactogenicity compared to homologous Astra and BNT vaccinations. [1] [2] [3] [4] 6 The combination of vector-and mRNA-based vaccines was initially thought to provide the benefits of both vaccination techniques. The hope was that this combination would yield the strong IgG responses known from mRNA vaccines as well as enhanced T cell responses. 40 Both responses appear to play an important role in vaccine-induced protection against SARS-CoV-2 infection, particularly in the early phase after vaccination. 21, 36 CD8 + cell responses in particular were expected to be increased through heterologous vaccination with Astra/BNT, as based on animal models and pathophysiological considerations. 40, 41 Several studies in smaller subpopulations appeared to support this hypothesis. 2, 3, 42 However, in our large data set, we were not able to confirm a general superiority of the Astra/BNT regime compared to homologous mRNA vaccination (BNT/BNT) in terms of T cell stimulation. At T2, booster vaccination with BNT significantly increased spike-directed IFN-γ release of T cells in individuals from the Astra/BNT regime as well as the BNT/BNT regime, with no statistically relevant difference between these two groups. All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in Booster vaccination with Astra had no discernible influence on IFN-γ release by T cells, suggesting that booster immunization in this subgroup is ineffective for increasing T cell-mediated immunity. This is in line with recent findings by Hillus et al. 6 , who analyzed spike-specific IFN--release of T cells in a small cohort and found that Astra booster vaccination did not further increase the T cell response in individuals prime vaccinated with Astra. Of note, Schmidt et al. 3 showed in a small study that all three vaccination regimes (Astra/Astra, Astra/BNT, BNT/BNT) induced polyfunctional T cells, which slightly differed in the induced CD4 + and CD8 + T cell subpopulations, distinguishable by their particular cytokine profiles. We did not account for different T cell subpopulations and can hence not exclude subtle functional advantages of a heterologous vaccination. We designed the COV-ADAPT study to assess correlations between immune responses from the first to the second vaccination. We found that the humoral (antispike-RBD-IgG) response at T1 was positively correlated with the response at T2 for both BNT-boostered groups, with a stronger effect measured in the Astra/BNT group. The correlation within the Astra/Astra group showed a similar effect but did not reach statistical significance. This may be attributed to the small group size (n=27). Thus, a strong or a weak initial humoral response appears to be predictive for the development of a high or a low titer of protective anti-spike-RBD-IgG at T2, respectively. Additionally, we found a strong and significant positive correlation between IFN- release by T cells at T1 and T2 for the Astra/Astra and the Astra/BNT groups. Thus, participants who started off with a strong response also showed strong responses at T2, while weak responses remained weak at T2. The positive correlation was not associated with an increased T cell response from T1 to T2 ( Figure 3A ) for the Astra/Astra group. This may be attributed to the fact that T cell activity depended heavily on the reaction to the prime vaccine and could not be further increased by the Astra booster. In contrast, in the Astra/BNT group T cell responses generally increased from T1 to T2, indicating a BNT-induced booster effect. On an aggregate level, humoral and cellular responses correlated with each other at T1 and T2, respectively. When looking at the particular vaccine regimes, a significant association between these responses could only be detected for the Astra/Astra and All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in The copyright holder for this this version posted December 14, 2021. ; https://doi.org/10.1101/2021.12.13.21267729 doi: medRxiv preprint the Astra/BNT group at T1. The loss of significance for all vaccination regimes at T2 could be attributed to a stronger augmentation auf anti-spike-RBD-IgG titers compared to the T cell responses. One may speculate that antibody titers have a higher capacity to increase as compared to the cellular compartment of the immune system, resulting in a loss of linear association. An additional analysis investigating the correlation between IFN- response at T1 and anti-spike-RBD-IgG at T2 showed a significant correlation for the Astra/BNT and BNT/BNT groups, suggesting that good T cell responses after T1 corresponded with good anti-spike-RBD-IgG responses at T2 ( Figure 5 ). This is in line with the notion that functional T helper cells as well as memory T cells are fundamental for launching a successful immune response. 43 Thus, an effective spike-directed T cell activation at T1 leads to increased anti-spike-RBD titers at re-exposure to the antigen. While quantitative immune responses did not differ between males and females, subjects of higher age and male sex showed lower avidity after the prime vaccination (Supplementary Table 2 ). Such general sex-dependent differences in immune responses might contribute to increased COVID-19 severity in men and older individuals, as has been observed in previous studies. 17 However, due to the semiquantitative nature of the test used here, all subjects reached the maximum test category "high avidity" at T2 (Figure 6 ). We therefore did not find a correlation between vaccination regimes and/or specific participant characteristics. Most individuals who received BNT as a booster developed a significant immune response, which was, by all quantitative parameters measured in this study, highly superior to immunity induced by Astra/Astra. Unexpectedly, there were no significant differences between the three vaccination regimens in regard to the neutralization indices at T1 and T2. A possible explanation is that the relative neutralization capacity had already been high (90%) after prime vaccination. However, in contrast to the Astra/Astra group, some individuals presented with a lack of neutralizing antibodies at T2 following booster vaccination with BNT. Additionally, there were hints at a more short-lived immune response after BNT booster even within the relatively short observation time, as compared to the Astra/Astra vaccination (Supplementary Figure 1 ). This would be in line with other studies showing a faster decline in humoral All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in The copyright holder for this this version posted December 14, 2021. ; https://doi.org/10.1101/2021.12.13.21267729 doi: medRxiv preprint responses following mRNA vaccinations compared to vector-based vaccines. 26, 31 It should be taken into account that the Astra carrier virus DNA remains in the organism in a transcriptionally active form for months, while maintaining activated CD8-T cells. 44 In contrast, persistence of mRNA in the organism is short (at least in mice 45 ), with a maximum of 10 days. It is conceivable that prolonged spike protein exposure in vectorbased vaccines enables the organism to increase the quality of the immune response over a longer time. In conclusion, our study suggests that an mRNA-based vaccine such as BNT should generally be chosen for booster vaccination rather than Astra due to its superiority in the elicited humoral and cellular immune response. This appears particularly important for those individuals with a poor immune response after prime vaccination with Astra. Our findings revealed an association between humoral and cellular immune responses following vaccination, and that a poor humoral immune response is unlikely to be mitigated by a strong cellular immune response. The distinct differences between vaccination regimes, as demonstrated in this study, should be taken into account for population-based vaccine programs. All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in The copyright holder for this this version posted December 14, 2021. ; https://doi.org/10.1101/2021.12.13.21267729 doi: medRxiv preprint Ethics approval and consent to participate: The COV-ADAPT study was approved by the ethics committee of the University Medical Center Göttingen (21/5/21). Informed consent was obtained from all participants and study design and study implementation were performed in accordance with the guidelines of Good Clinical Practice (ICH 1996) perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in (C) Regression of TC_T2 (Anti-spike IGRA after the second blood sample) on TC_T1 (Anti-spike IGRA after the first blood draw) controlling for age, sex, and time between second vaccination and T2. All rights reserved. No reuse allowed without permission. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in is based ✓ *Give information separately for exposed and unexposed groups. perpetuity. preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in The copyright holder for this this version posted December 14, 2021. ; https://doi.org/10.1101/2021.12.13.21267729 doi: medRxiv preprint Immunogenicity and reactogenicity of BNT162b2 booster in ChAdOx1-S-primed participants (CombiVacS): a multicentre, openlabel, randomised, controlled, phase 2 trial Immune responses against SARS-CoV-2 variants after heterologous and homologous ChAdOx1 nCoV-19/BNT162b2 vaccination Immunogenicity and reactogenicity of heterologous ChAdOx1 nCoV-19/mRNA vaccination Safety and immunogenicity of heterologous versus homologous prime-boost schedules with an adenoviral vectored and mRNA COVID-19 vaccine (Com-COV): a single-blind, randomised, non-inferiority trial Immunogenicity and efficacy of heterologous ChadOx1/BNT162b2 vaccination Safety, reactogenicity, and immunogenicity of homologous and heterologous prime-boost immunisation with ChAdOx1 nCoV-19 and BNT162b2: a prospective cohort study The Effects of Heterologous Immunization with Prime-Boost COVID-19 Vaccination against SARS-CoV-2 WHO Declares COVID-19 a Pandemic A pneumonia outbreak associated with a new coronavirus of probable bat origin A Novel Coronavirus from Patients with Pneumonia in China The Johns Hopkins Coronavirus Resource Center (CRC). 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Heterologous vaccination regimens with self-amplifying RNA and adenoviral COVID vaccines induce robust immune responses in mice To mix or not to mix? A rapid systematic review of heterologous prime-boost covid-19 vaccination Targets of T Cell Responses to SARS-CoV-2 Coronavirus in Humans with COVID-19 Disease and Unexposed Individuals Adenoviral vectors persist in vivo and maintain activated CD8+ T cells: implications for their use as vaccines In the interest of transparency, we ask you to disclose all relationships/activities/interests listed below that are related to the content of your manuscript. "Related" means any relation with for-profit or not-for-profit third parties whose interests may be affected by the content of the manuscript. Disclosure represents a commitment to transparency and does not necessarily indicate a bias. 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