key: cord-0687272-ch9gfgal
authors: Puente, Nuria; Fayos, Marina; Pablo-Marcos, Daniel; Valero Díaz de Lamadrid, Carmen
title: Oropharyngeal persistence of SARS-CoV-2: Influence of viral load
date: 2022-05-02
journal: Med Clin (Engl Ed)
DOI: 10.1016/j.medcle.2021.10.014
sha: 28a9ff18b12ee3d2a1cdc3995fed13505e684da1
doc_id: 687272
cord_uid: ch9gfgal

nan

Oropharyngeal persistence of SARS-CoV-2: Influence of viral load Nuria Puente 1 , Marina Fayos 1 , Daniel Pablo-Marcos 2 Departamento de Medicina Interna 1 . Servicio de Microbiología 2 . Hospital Universitario Marqués de Valdecilla. Santander. España

Los autores declaran no tener ningún conflicto de intereses.

To the Editor: Several clinical factors have been associated with oropharyngeal persistence of SARS-CoV-2 1 . Our study included the viral load determination by quantitative PCR (Exact Diagnostics SARS-CoV-2 Standard; Bio-Rad, Texas, USA) of 33 COVID-19 patients with persistent PCR in nasopharyngeal samples░>░4 weeks and 33 controls, adjusted for age and sex, who tested negative before. Values are expressed as Log10 mean viral load (N and R genes) and cycle threshold (Ct) values. The Clinical Research Ethics Committee (CREC) of Cantabria approved the study. Student's t-test or Mann-Whitney U were used to compare quantitative variables, and chi-square or Fisher's test for qualitative variables. All analyses were performed using SPSS 23.0 software (Chicago, IL, USA). A p value ░<░0.05 was considered statistically significant.

The viral load of COVID-19 patients with mild disease (outpatients) and persistent SARS-CoV-2 was significantly higher than that of their controls in copies/ml (Log10: 7.04░±░1.81 copies/ml vs. 5.15░±░2.14 copies/ml; p░=░0.018) and Ct of the N gene (25.7░±░5.6 versus 31.3░±░6.7 in controls; p░=░0.02). Their clinical profile showed no peculiarities (table 1 table 1) . Hospitalized patients with persistent SARS-CoV-2 (49░±░20 days) had the same viral load as their controls (Log10: 6.21░±░2.06 copies/ml vs. 5.98░±░1.97 copies/mL; p░=░0.73 and Ct values of the N and R genes) and there were no differences in terms of their clinical characteristics (Table 1) . Viral shedding in respiratory samples varies from 2 to 3 weeks after the onset of symptoms, but it has been reported up to 83 days later 2 . Various clinical factors are related to this fact, including male gender, age over 65, the use of invasive mechanical ventilation, the presence of immunodeficiency or diabetes 2 . Some studies find that persistence is more common in seriously ill hospitalised patients with high comorbidity 3 ; however, others associate it with asymptomatic cases 2 . The use of corticosteroids and lopinavir/ritonavir also seems to be associated 4,5 . Our study has the limitations of observational studies and we do not know the clinical translation of the persistence of SARS-CoV-2; however, we consider it important to continue analysing the problem of prolonged shedding and fluctuations of the virus.

The authors declare that they have no conflicts of interest. 

Factors associated with prolonged viral RNA shedding in patients with COVID-19

Prolonged SARS-CoV-2 detection and reversed RT-PCR results in mild or asymptomatic patients