key: cord-0722044-33vlcdbj authors: Salık, Fikret; Uzundere, Osman; Bıçak, Mustafa; Akelma, Hakan; Akgündüz, Mesut; Korhan, Zeki; Kandemir, Deniz; Kaçar, Cem Kıvılcım title: Liver function as a predictor of mortality in COVID-19. Retrospective study date: 2021-10-06 journal: Ann Hepatol DOI: 10.1016/j.aohep.2021.100553 sha: c6ddcb8121294d555300b79a9bf41c8cdc03801d doc_id: 722044 cord_uid: 33vlcdbj INTRODUCTION AND OBJECTIVES: In many studies, varying degrees of liver damage have been reported in more than half of the COVID-19 patients. The aim of this study is to determine the effect of liver biochemical parameters abnormality on mortality in critical COVID-19 patients who have been followed in the ICU since the beginning of the pandemic process. MATERIALS AND METHODS: In this study 533 critical patients who admitted to the ICU due to COVID-19 were included. The patients were divided into three groups according to their ALT, AST, and total bilirubin levels at their admission to the ICU. Group 1 was formed of patients with normal liver biochemical parameters values; Group 2 was formed of patients with liver biochemical parameters abnormality; Group 3 was formed of patients with liver injury. RESULTS: 353 (66.2%) of all patients died. Neutrophil, aPTT, CRP, LDH, CK, ALT, AST, bilirubin, procalcitonin and ferritin values in Group 2 and Group 3 were found to be statistically significantly higher than Group 1. It was detected that the days of stay in ICU of the patients in Group 1 was statistically significantly longer than others group. It was found that the patients in Groups 2 and 3 had higher total, 7-day, and 28-day mortality rates than expected. CONCLUSIONS: The study showed that liver disfunction was associated with higher mortality and shorter ICU occupation time. In a number of studies, varying degrees of liver damage have been noted in more than half of the patients who diagnosed COVID-19 [1, 2] . It has been shown that the SARS-CoV-2 receptor, called ACE 2, is also present highly in bile duct cells except alveolar epithelial cells. [3] [4] . Some studies support that SARS-CoV-2 can also infect bile duct cells through this receptor and cause liver biochemical parameters abnormality [5] . In previous studies, it has been noted that 14-78% of COVID-19 patients have an increase in liver biochemical parameters [1, 2, [6] [7] [8] [9] . Additionally, microvesicular steatosis, lobular activity and portal activity were demonstrated in liver biopsy specimens of one patient who died from COVID-19 [10] . This suggests that SARS-CoV-2 may have caused liver damage. Liver dysfunction that develops in these patients can cause to failure of liver and death [11] . For this reason, it is important to investigate liver damage in COVID-19 cases. Considered the highly contagious and pathogenic nature of SARS-CoV-2 and the high incidence of liver damage, evaluation of liver function in COVID-19 patients is important [5] . Previous studies have reported an association between liver function and duration of hospital stay [5] , risk of progression to severe COVID-19 [7] [8] [9] , and mortality [12] . The aim of this study is to retrospectively determine the effect of liver biochemical parameters abnormalities on mortality in critical patients with COVID-19 who have been followed in the ICU since the beginning of the pandemic process. Critical COVID-19 patients who admitted to the ICU of our hospital between April 2020 and October 2020 and who was confirmed with real-time reverse transcriptasepolymerase chain reaction (RT-PCR) test were included in this retrospective cohort study. The necessary permits have been taken from The Republic of Turkey Ministry of Health Scientific Research Platform (11/20/2020) Critical patients who had COVID-19 and admitted to the ICU on the dates specified, 18 > age, in serious need of oxygen support according to WHO [13] and the temporary guidelines of The Republic of Turkey Ministry of Health Scientific Committee [14] , (respiratory rate > 30 / min. and/or severe respiratory distress and/or oxygen saturation at room air < 90% (the patient receiving oxygen PaO2 / FiO2 < 300); bilateral diffuse pneumonia findings detected on chest images; developed or had severe pneumonia, ARDS, sepsis, septic shock and acute renal failure, were included. Patients who 18 < age, pregnant, with a history of liver disease or chronic viral hepatitis infection, whose data are not fully available in the hospital system or the patient file records, with mild-moderate symptoms, no respiratory distress, and no signs of diffuse pneumonia in chest radiography or tomography, and non COVID-19 were excluded. The patients' age, gender, comorbidity, and complaints were recorded. Acute Physiology and Chronic Health Evaluation II (APACHE II) and Sequential Organ Failure Assessment (SOFA) scores, hemogram parameters (white blood cell (WBC), neutrophil, lymphocyte, platelet count), coagulation parameters (prothrombin time (PTZ), Activated Partial Thromboplastin Time (aPTT), and D-dimer), blood biochemistry values (C-reactive protein (CRP), lactate dehydrogenase (LDH), creatine kinase (CK), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, direct bilirubin, and indirect bilirubin; c702-502 autoanalyzer, Roche, Ros-bach, Germany), procalcitonin and ferritin levels were recorded at the time of the ICU admission. In addition, the ICU days, 7-day, 28day, and total mortality were recorded. Clinical data were collected from the hospital's computer system. Patient data were rechecked for erroneous data before the last data entry and entered into a computerized database. All published literature which analyzed liver biochemical parameters in COVID-19 patients was examined through databases and shown in Table 1 [5, 6, 11, . Based on previous studies, liver biochemical parameters abnormalities were defined as the elevation of the following liver enzymes in serum: ALT > 40 U / L, AST > 40 U / L, and total bilirubin> 1.20 mg/d l. As in a previous study, we defined ALT and/or AST over three times upper limits of normal (ULN), and/or total bilirubin over two times ULN as liver injury [7] . Patients were divided into three groups according to liver biochemical parameters values at their admission to the ICU. Group 1 was formed of patients with normal liver biochemical parameters values; Group 2 was formed of patients with liver biochemical parameters abnormality; Group 3 was formed of patients with liver injury. All three groups were compared in terms of clinical characteristics, APACHE II and SOFA scores, laboratory values, days of ICU stay, 7-day, 28-day, and total mortality. SPSS 22 .0 for Windows program (SPSS Inc., Chicago, IL, USA) was used for statistical analysis. Numerical data were expressed as means with standard deviation. Categorical data as frequencies with percentages. Comparison of categorical data between groups was made using the chi-square test and the results were given as n%. Whether the numerical data fit the normality distribution was evaluated using the Kolmogorov-Smirnov test. The Kruskal Wallis test was used in the comparison of the groups, as the numerical data did not conform to the normal distribution. Student-t and Mann-Whitney U tests were used to compare groups in pairs. In all comparisons, p < 0.05 was considered significant. In the study, the data of 567 patients in total were accessed. After exclusion criteria, 34 patients were excluded, and the study was completed with 533 patients. The patients' mean age was 69.2 ± 14.8 years. 283 (53.1%) patients were male and 250 (46.9%) were female. 401 (75.2%) patients had at least one comorbidity and the most common comorbidities were hypertension (218, 40.9%) and diabetes (151, 28.3%). Between the dates of the study, 353 of all patients died. The mortality rate was found to be 66.2%. The average stay in the ICU was 11.3 ± 10.7 days. The patients' demographic and clinical datas are represented in Table 2 . When the groups were compared in terms of demographic and clinical characteristics, it was found that the rates of liver biochemical parameters abnormality and liver injury were higher in the male patients than in the female patients (p < 0.001). In addition, those with liver damage were found to have statistically significantly higher SOFA scores (p < 0.001) ( Table 2 ). When the groups were compared in terms of laboratory values at the time of first admission to the ICU, the neutrophil, aPTT, CRP, LDH, CK, ALT, AST, total bilirubin, direct bilirubin, indirect bilirubin, procalcitonin, and ferritin values were statistically significantly higher in Group 2 and Group 3 than Group 1 (p = 0.047 for neutrophil; p = 0.025 for aPTT; p = 0.033 for CRP; p 0.001 for other values). Although most laboratory values were found to be higher in Group 3 than Group 2, mean CRP and indirect bilirubin values were higher in Group 2 than Group 3, but this difference was not statistically significant in the dual analysis performed (p = for CRP. 0.36; p = 0.87 for indirect bilirubin) ( Table 2) . It was found that the length of ICU stay of the patients were statistically significantly longer in Group 1 than in Groups 2 and 3 (p = 0.033). When Group 2 and Group 3 were compared, it was found that the duration of stay in the ICU of the patients were statistically significantly shorter in Group 3 than in Group 2 (p = 0.042) ( Table 2 ). It was found that the patients in Groups 2 and 3 had higher total, 7-day, and 28-day mortality rates than expected (p-values: 0.004; 0.001; 0.003, respectively) ( Table 3 ). In this study, liver biochemical parameters abnormality and liver injury were detected in 52% of critical COVID-19 patients during the stay in the ICU. Neutrophil, CRP, LDH, CK, procalcitonin, and ferritin values were found to be higher in patients with liver biochemical parameters abnormality and liver injury compared to patients with normal liver biochemical parameters. In addition, it was determined that patients with liver biochemical parameters abnormality and liver injury had a shorter stay in the ICU than expected, and higher total, 7day, and 28-day mortality rates compared to patients with normal liver biochemical parameters. Liver damage in COVID-19 patients can be attributed to a number of secondary effects of the disease as well as the primary infection. Alternative etiologies such as systemic inflammatory response and cytokine storm associated with COVID-19, drug-induced liver damage, hypoxia, hepatic ischemia, and shock can be counted among these secondary effects [15, 38] . Compared to the normal liver biochemical parameters group, we found that patients with liver biochemical parameters abnormality and liver injury had higher levels of neutrophils, CRP, procalcitonin, and ferritin, which may be associated with an immune response after virus infection. This suggests that the inflammatory response also contributes to the occurrence of COVID-19 associated liver damage. In a systematic review of 107 studies from various countries, Kulkarni found that the rate of high AST value was higher in ICU patients (62%) than non-ICU patients (25%) (mean values respectively 44.0 U/L vs 34.0 U/L) [6] . In this study, 43.3% of critical COVID-19 patients were found to have liver biochemical parameters abnormality and 8.6% were found to have liver injury during the stay in the ICU. The results of our study are in line with previous studies. It has been stated in some publications that the rate of male patients is higher in severe COVID-19 compared to female patients [12, 21, 23, 41, 43] . In addition, it has been reported that patients with abnormal liver biochemical parameters and liver injury in COVID-19 patients are mostly male patients, but the underlying mechanism is not clear [5, 7] . In our study, 53.1% of our total number of patients were male, and in accordance with previous studies, it was determined that the groups with abnormal liver biochemical parameters and liver injury were mostly male patients. In studies conducted on COVID-19 patients, the length of hospital stay was longer in patients with abnormal liver biochemical parameters at the time of admission than in patients with normal liver biochemical parameters [5, 11] . Jiang et al. concluded that liver damage would lead to problems in the immune system and thus delay the clearance of the virus. This can be explained by the prolonged hospital stay, the more time needed for liver function to recover, or the failure to eradicate the virus. In the same study, the duration of hospitalization in patients with liver damage in the critically patient group was numerically higher than in patients without liver damage, but this was not statistically significant [11] . In our study, it was found that the time of stay at the ICU in patients with liver biochemical parameters abnormality and liver injury were shorter than patients with normal liver biochemical parameters. We think that hospitalization periods in critically patients who had COVID-19 are affected by many factors, and the high mortality rates in the liver biochemical parameters abnormality and liver injury groups may cause a short stay in the ICU. Many studies have reported that abnormal liver biochemical parameters, especially elevated AST and ALT, are associated with increased disease severity and mortality in COVID-19 patients [7,12,24,25,27,44,] . Yip et al. stated that ALT / AST elevation and acute liver damage in patients with COVID-19 were independently associated with mortality and that such biochemical changes had important consequences [21] . Huang et al. stated that dynamic changes of ALT and AST levels in COVID-19 patients were more pronounced in patients with liver function damage and in patients who died; The patients with AST > three times the upper limit of normal (ULN) have the highest risk of death and mechanical ventilation [45] . Lei et al. reported that elevated ALT, AST, ALP, and total bilirubin levels in COVID-19 patients are associated with an increased risk of death and that elevated AST among these liver enzymes is associated with the highest risk of death [12] . In their study, Chen et al. noted that ALT, AST, GGT, ALP, and total bilirubin concentrations were significantly higher in patients who died than in healed patients; and that approximately 52% of patients who died and 16% of those who recovered had high AST levels [29] . Again Kulkarni et al., in their meta-analysis, found a higher rate of abnormal liver biochemical parameters results in the non-survive group at the first admission compared to the survive group in COVID-19 patients [39] . In our study, in line with previous studies, it was found that in patients with liver biochemical parameters abnormality and liver injury had higher total, 7day, and 28-day mortality rates compared to patients with normal liver biochemical parameters. This study has some limitations. Our data were not capable of evaluating the causality of liver damage and poor clinical outcomes associated with COVID-19. As a result, liver dysfunction evaluated by biochemical blood analysis (AST, ALT, and total bilirubin levels) is common in critical COVID-19 patients followed in the ICU. Abnormal liver biochemical parameters are closely related to an increased risk of mortality in critically ill COVID-19 patients. Therefore, these indicators should be closely monitored during the stay in the ICU and special attention should be paid to liver damage. FS is the first author. Each author either made substantial contributions to the con-ception or design of the work. FS, CKK, and OU wrote the paper. Each author involved in the acquisition, analysis, or interpretation of data for the work. FS, MB and OU were involved in data clean-ing, mortality follow-up, and verification. Each author drafted the manuscript or revised it critically for important intellectual con-tent; and provided final approval of the version to be published. All authors have read and approved the final manuscript. FS and CKK are the study guarantors. The trial was registered with clinicaltrials.gov (NCT04669509) No funding was used toward the development of this study and there are no financial interests or conflicts of interests related to this work. 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The authors declare no conflict of interest.