key: cord-0722583-ev7z1gl8
authors: Wang, Jiongjiong; Prinz, Richard A.; Liu, Xiufan; Xu, Xiulong
title: In Vitro and In Vivo Antiviral Activity of Gingerenone A on Influenza A Virus Is Mediated by Targeting Janus Kinase 2
date: 2020-10-08
journal: Viruses
DOI: 10.3390/v12101141
sha: eb1fbe5d0f3be10c3eb977ed723b94673926d4b1
doc_id: 722583
cord_uid: ev7z1gl8

Janus kinase (JAK) inhibitors have been developed as novel immunomodulatory drugs and primarily used for treating rheumatoid arthritis and other inflammatory diseases. Recent studies have suggested that this category of anti-inflammatory drugs could be potentially useful for the control of inflammation “storms” in respiratory virus infections. In addition to their role in regulating immune cell functions, JAK1 and JAK2 have been recently identified as crucial cellular factors involved in influenza A virus (IAV) replication and could be potentially targeted for antiviral therapy. Gingerenone A (Gin A) is a compound derived from ginger roots and a dual inhibitor of JAK2 and p70 S6 kinase (S6K1). Our present study aimed to determine the antiviral activity of Gin A on influenza A virus (IAV) and to understand its mechanisms of action. Here, we reported that Gin A suppressed the replication of three IAV subtypes (H1N1, H5N1, H9N2) in four cell lines. IAV replication was also inhibited by Ruxolitinib (Rux), a JAK inhibitor, but not by PF-4708671, an S6K1 inhibitor. JAK2 overexpression enhanced H5N1 virus replication and attenuated Gin A-mediated antiviral activity. In vivo experiments revealed that Gin A treatment suppressed IAV replication in the lungs of H5N1 virus-infected mice, alleviated their body weight loss, and prolonged their survival. Our study suggests that Gin A restricts IAV replication by inhibiting JAK2 activity; Gin A could be potentially useful for the control of influenza virus infections.

Influenza is a highly contagious acute respiratory illness [1] . The disease is often presented as seasonal influenza epidemics and causes several hundred thousand deaths per year. In the United States alone, influenza accounts for an average of 36,000 deaths and >226,000 hospitalizations annually [1] . Four influenza pandemics in the past century, namely the H1N1 Spanish flu (1918), the H2N2 Asian flu (1957) , the H3N2 Hong Kong flu (1968) , and the H1N1 swine flu (2009), claimed millions of lives [2, 3] . High morbidity and mortality, as well as the risk of an influenza pandemic, pose a major threat to public health [4] . Its causative agent, influenza virus, belongs to the Orthomyxoviridae family. Its genome

The experiments were approved by the Institutional Biosafety Committee of Yangzhou University. All experiments involving live virulent H5N1 viruses and animals were carried out in a P3-level biosafety lab certified by the Ministry of Agriculture, China. The protocols for the animal experiments were approved by the Jiangsu Administrative Committee for Laboratory Animals (approval number: SYXK-SU-2017-0007, 3 March, 2007) , and complied with the guidelines of Jiangsu laboratory animal welfare and ethics of Jiangsu Administrative Committee of Laboratory Animals. 

Ruxolitinib and PF-4708671 were purchased from Selleck Inc. (Shanghai, China). Gin A and antibodies against phospho-JAK2Y1007/Y1008 (sc-16566-R), β-actin (sc-47778), and GAPDH (sc-166574) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Antibodies against phospho-tyrosine (P-Y-1000, #8954), STAT3 (#9139), phospho-STAT3Y705 (#9145), JAK2 (#3230), AKT (#4691), phospho-AKTS473 (#4060), S6K1 (#2708), phospho-S6K1T389 (#9234), S6 (#2217), and phospho-S6S235/236 (#4858) were purchased from Cell Signaling Technology (Danvers, MA, USA). An anti-nucleoprotein (NP) polyclonal antibody was prepared by immunizing mice with purified recombinant NP protein. An anti-M1 monoclonal antibody was prepared in our lab. An anti-PB2 antibody (#GTX125926) was purchased from GeneTex Inc. (Irvine, CA, USA).

Madin-Darby canine kidney (MDCK), A549 (a human lung cancer cell line of alveolar epithelial cell origin), 293T (a human embryonic kidney cell line), and DF1 (a chicken fibroblast cell line) cells were purchased from the American Tissue Culture Collection (Manassas, VA, USA). Cells were grown in DMEM containing 10% fetal bovine serum (FBS). The A/mallard/Huadong/S/2005 H5N1 virus (SY strain), a moderately pathogenic avian influenza strain, was isolated from poultry [20] . The virus was plaque purified three times in MDCK cells. A/PR8/34 (PR8) virus, a murine-adapted H1N1 subtype, was kindly provided by Dr. Liqian Zhu, Yangzhou University. A/California/04/09 (CA09), a pandemic H1N1 subtype of swine-origin, was kindly provided by Dr. Jinhua Liu, China Agricultural University. Avian Ck/SH/F/98 (F98) virus, an H9N2 virus subtype has been reported previously [21] . All IAV strains were propagated in 10 day-old specific-pathogen-free embryonic chicken eggs. Virus titers were measured by a 10-fold serial dilution (10 1 to 10 9 ), and each dilution (10 5 -10 9 ) was used to infect chicken embryonic fibroblast (CEF) or MDCK cells. The 50% tissue culture infection dose (TCID 50 /mL) was calculated according to the Reed and Muench method. The IC 50 values of Gin A required to inhibit IAV replication were calculated by using GraphPad Prism software (Version 8.0.0 for Windows, GraphPad Software, San Diego, CA, USA).

To determine the effect of Gin A on virus growth, four cell lines (293T, A549, MDCK, and DF1) were infected with 0.01 multiplicity of infection (MOI) of H1N1, H5N1, or H9N2 virus and then incubated in the presence of various concentrations of Gin A (0, 10, 25, 50 µM). For H1N1 and H9N2 virus infection, tosyl-phenylalanyl-chloromethyl-ketone-treated trypsin (Sigma, St. Louis, MO, USA) (2 µg/mL) was added into the media during and after virus infection. The conditioned media were collected at 18, 24, or 36 hours post-infection (hpi). The TCID 50 values were determined on MDCK cells according to our previous publications [20] . Data represent the mean ± standard deviation (SD) of four independent experiments.

The 293T cells seeded in 6-well plates were infected with 0.01 MOI H5N1 virus and then incubated in the absence or presence of Gin A (0, 10, 25, 50 µM) for 16 h. Total RNA was extracted from H5N1 virus-infected cells by using a Qiagen RNA extraction kit. Viral RNA levels were measured by using a one-step reverse-transcription FRET-PCR to amplify the M gene according to our recent publication [22] .

The 293T cells were transfected with 100 ng of luciferase reporter plasmid (p-Luci, kindly provided by Dr. Jinhua Liu) and the mixture of pcDNA3.1(+) plasmid constructs expressing the polymerase subunits PB2, PB1, PA, and NP genes from the A/mallard/Huadong/S/2005 H5N1 virus (200 ng each), Viruses 2020, 12, 1141 4 of 18 and 20 ng internal control Renilla plasmid using Polyfect Transfection Reagent (Qiagen, Germantown, MD, USA). After incubation for 24 h, the cells were treated with dimethyl sulphoxide (DMSO) (0.5%) or with the indicated concentrations of Gin A (0, 10, 25, 50 µM) for another 24 h. The cells were harvested and analyzed for luciferase activity by using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA).

The 293T, DF1, MDCK, and A549 cells seeded in 6-well plates were infected with H5N1, H1N1, or H9N2 (0.1 MOI each), and then incubated in the absence or presence of the indicated concentrations of various inhibitors for 8 h in the media containing 1% FBS. The cells were harvested and lysed in NP-40 lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% NP-40, 5 mM EDTA, 5 mm EGTA, 1 mM NaF, and 2 mM sodium vanadate, the cocktail of protease inhibitors (1×) (Pierce Chemical Co., Rockford, IL, USA), 2 mM sodium pervanadate). Cell lysates were prepared and analyzed for the expression of viral proteins (PB2, NP, and M1) or cellular proteins with their specific antibodies, followed by horseradish peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) and SuperSignal Western Pico-enhanced chemiluminescence substrate (Pierce Chemical Co., Rockford, IL, USA). The density of the bands was analyzed by using an NIH Image-J software and normalized by the arbitrary units of their corresponding total proteins or β-actin. Quantified results were presented as the mean ± standard deviation (SD) from three experiments in bar graphs.

The use of animals was approved by the Institutional Animal Care and Use Committee of the College of Veterinary Medicine, Yangzhou University. Female C57BL/6 mice were purchased from the Center of Experimental Animals of the College of Veterinary Medicine, Yangzhou University. All mice were maintained on a 12 h light/dark cycle and housed in ventilated cages at an ambient temperature of 23 • C. Mice were fed ad libitum on a normal chow diet (NCD). Gin A was first dissolved in dimethyl sulfoxide (DMSO) and then diluted in polyethylene glycol (PEG) 200 (Tokyo Chemical Industry, Inc., Tokyo, Japan). Mice (8 weeks old) (5 mice per group) were treated with Gin A (20 mg/kg/day) by gavage. Twelve hours after the first treatment, mice under diethyl ether anesthesia were intranasally mock-infected with PBS or infected with the H5N1 virus (1 × 10 5 pfu in 50 µL PBS) by instillation. The H5N1 virus is a moderately pathogenic strain in mice. The dose was determined based on a previous publication from our group [23] . Mice were then treated with the same dose daily for three or five days. Mice were sacrificed by CO 2 inhalation. Lung tissues were homogenized in a radioimmunoprecipitation assay (RIPA) lysis buffer (Cell Signaling Technology, Danvers, MA, USA) for Western blot or in PBS for analyzing virus loads. To determine the therapeutic effect of Gin A, mice (10 mice per group) infected with H5N1 virus were treated daily with Gin A (5 mg/kg body weight in 20 µL PEG 200) by intranasal instillation for 7 days. To avoid physical irritation caused by gavage and poor drug absorbance in the gastrointestinal tract of the H5N1 virus-infected mice, Gin A was given intranasally at a low dose in this experiment, since the drug, administered this way, directly goes to the lung where the virus replicates. Mice in control groups were treated with PEG 200. Mice were monitored daily for body weights and survival for 2-3 weeks and were humanely sacrificed by CO2 inhalation when they became moribund or when the loss of body weight decreased by >25%. The body weights of a small fraction of mice dropped beyond 25% in the last day before they were sacrificed. The last-day data of these mice were not included.

The 293T cells were transiently transfected with the empty vector or pBABE-JAK2, a retroviral vector encoding JAK2 tagged with a fragment of yellow fluorescence protein (YFP) (kindly provided by Dr. Eric Chang, Baylor College of Medicine, Houston, TX, USA) [24] . After incubation for 36 h, the cells were left uninfected or infected with the indicated MOI of the H5N1 virus and then incubated Viruses 2020, 12, 1141 5 of 18 for 12 h. Alternatively, the cells were infected with the H5N1 virus (0.1 MOI) and then incubated in the absence or presence of various concentrations of Gin A (0, 10, 25, 50 µM) for 12 h. Cell lysates were prepared and analyzed for JAK2 and STAT3 phosphorylation and the levels of viral NP and M1 proteins. The virus titers in the conditioned media were collected and analyzed for the TCID 50 values. Relative virus titers were calculated as the percent of controls. The results represent the mean ± SD of three independent experiments.

To exclude the possibility that the inhibitory effect of Gin A on virus replication was due to inhibition of cell proliferation, 293T cells were incubated with various concentrations of Gin A for 12 h and then analyzed for cell proliferation by using a CellTiter-Glo kit (Promega, WI, USA). To determine how selectively Gin A inhibited virus replication versus its cytotoxicity in the cells infected with IAV, 293T, A549, MDCK, and DF-1 cells seeded in 96-well plates with an approximately 80% confluence were incubated in the presence of various concentrations (0, 12.5, 25, 50, 100, 200 µM) of Gin A for 24 h. Cell viability was measured by using a CellTiter-Glo kit as described above. The IC 50 values for cytotoxicity (C.C.) were calculated. The S.I. values were calculated by dividing the C.C. values with the IC 50 values of Gin A required to inhibit virus replication.

Differences in virus titers, viral mRNA levels, the arbitrary units of scanned Western blot band density, and cell proliferation index were statistically analyzed by using an unpaired Student t test. Differences in the body weights of untreated and Gin A-treated mice were analyzed using a repeated measures analysis of variance (ANOVA) test. Differences in the survival of untreated and Gin A-treated mice were statistically analyzed by using a Log-Rank test. A p value of < 0.05 was considered statistically significant. All statistics were analyzed with SigmaPlot 11 software (Systat Software, Inc., San Jose, CA, USA).

We first assessed the ability of Gin A to inhibit IAV replication in 293T cells, a human embryonic kidney cell line. The antiviral activity of Gin A was largely studied on the avian A/mallard/Huadong/S/2005 (SY) strain, a zoonotic virus of the H5N1 subtype with modest pathogenicity. The 293T cells infected with H5N1 virus (0.01 MOI) were incubated in the absence or presence of the indicated concentrations of Gin A (10-50 µM) for 18, 24, or 36 h. As shown in Figure 1B dose-dependently lowered the levels of the M1 gene of the H5N1 virus ( Figure 1F ). Consistently, Gin A also significantly decreased viral RNA polymerase activity in 293T cells in a luciferase reporter assay ( Figure 1G ). We then determined if the antiviral activity of Gin A was due to its anti-proliferative or cytotoxic effects. The 293T, A549, MDCK, and DF1 cells incubated in the presence of the indicated concentrations of Gin A for 12 h did not display any significant cytotoxicity or retarded cell growth ( Figure 1H ). After incubation for 24 h, the cells were incubated in the absence or presence of the indicated concentrations of Gin A for another 24 h. Cells were harvested and analyzed for luciferase activity in a plate reader. The results represent the mean ± SD from one of three experiments with similar results, each was done in triplicate; (H) Gin A has little effect on 293T cell proliferation. The 293T cells seeded in 96-well plates were incubated in the absence or presence of the indicated concentrations of Gin A for 12 h. Cell proliferation was analyzed as described in the Materials and Methods. Data are the mean ± SD of three experiments. * p < 0.05; ** p < 0.01, compared to the untreated controls. Then, we determined if Gin A also reduced the viral protein levels of three IAVs in four cell lines.

Then, we determined if Gin A also reduced the viral protein levels of three IAVs in four cell lines. As shown in Figure 2 , Gin A dose-dependently reduced the levels of the NP and M1 proteins in the MDCK, 293T, A549, and DF-1 cells infected with three IAV subtypes. Notably, Gin A reduced the levels of the M1 protein more profoundly than that of NP protein. Gin A lowered the viral protein levels of three IAV subtypes almost equally in these four cell line lines. We also examined the antiviral 

Then, we determined if Gin A also reduced the viral protein levels of three IAVs in four cell lines. As shown in Figure 2 , Gin A dose-dependently reduced the levels of the NP and M1 proteins in the MDCK, 293T, A549, and DF-1 cells infected with three IAV subtypes. Notably, Gin A reduced the levels of the M1 protein more profoundly than that of NP protein. Gin A lowered the viral protein levels of three IAV subtypes almost equally in these four cell line lines. We also examined the antiviral activity of Gin A on the CA09 virus, a pandemic H1N1 strain of swine-origin. The virus titers of this strain in the conditioned media of the 293T cells were too low to be detected. Western blot analysis revealed that Gin A reduced the levels of the NP and M1 proteins of this strain as effectively as those of the H1N1 PR8 strain ( Figure S1 ). Immunofluorescence staining revealed that the fluorescent signals of the viral HA, M1, and NP proteins in H5N1 virus-infected 293T cells in the presence of Gin A (25 µM) were significantly reduced ( Figure S2 ). This suggests that the antiviral activity of Gin A is indeed due to the restriction of virus replication but not due to its cytotoxic effect on 293T cells.

Viruses 2020, 12, x FOR PEER REVIEW 7 of 18 activity of Gin A on the CA09 virus, a pandemic H1N1 strain of swine-origin. The virus titers of this strain in the conditioned media of the 293T cells were too low to be detected. Western blot analysis revealed that Gin A reduced the levels of the NP and M1 proteins of this strain as effectively as those of the H1N1 PR8 strain ( Figure S1 ). Immunofluorescence staining revealed that the fluorescent signals of the viral HA, M1, and NP proteins in H5N1 virus-infected 293T cells in the presence of Gin A (25 μM) were significantly reduced ( Figure S2 ). This suggests that the antiviral activity of Gin A is indeed due to the restriction of virus replication but not due to its cytotoxic effect on 293T cells. 

Gin A is a dual inhibitor of JAK2 and S6K1 [19] . Here, we tested if Gin A also inhibited the activity of JAK2 and S6K1 in IAV-infected cells. We first examined the ability of Gin A to inhibit JAK2 and STAT3 tyrosine phosphorylation in H5N1 virus-infected 293 T cells. H5N1 virus infection did not significantly increase JAK2 tyrosine phosphorylation in 293T cells at 0.5 ( Figure 3A ) or 8 hpi ( Figure 3B ). Interestingly, STAT3 tyrosine phosphorylation was weakly increased at 0.5 hpi but slightly decreased at 8 hpi, a phenomenon consistent with the observation in a prior study [25] . Gin A dose-dependently inhibited JAK2 and STAT3 tyrosine phosphorylation in uninfected or H5N1 virus-infected 293T cells at 0.5 or 8 hpi ( Figure 3A,B) . Consistently, Gin A also inhibited JAK2 and STAT3 tyrosine phosphorylation in 293T cells infected with H1N1 ( Figure 3C ) or H9N2 ( Figure 3D ) virus at 8 hpi. Western blot analysis with an antibody against phospho-tyrosine revealed that Gin A inhibited the total protein tyrosine phosphorylation ( Figure 3E ). Consistent with two previous studies showing that S6K1 inhibitors induce the feedback activation of the PI-3 kinase pathway [26, 27] , Gin A decreased S6 phosphorylation but increased AKT phosphorylation in a dose-dependent manner ( Figure 3F ). Of note, Gin A should also increase S6K1 phosphorylation but instead inhibited S6K1 phosphorylation, an observation that was also made in our recent study, suggesting its ability to inhibit mTOR activity as well [27] .

Recent studies have shown that JAK1 and JAK2 play important roles in IAV replication [14, 28, 29] . We hypothesized that Gin A may inhibit IAV replication by inhibiting JAK2 activity. We investigated if Ruxolitinib (Rux), a JAK inhibitor, also inhibited IAV replication. Ruxdose dependently reduced the levels of the M1 and NP proteins ( Figure 4A ) in the lysates of 293T cells infected with the H5N1 virus and decreased the virus titers in the conditioned media ( Figure 4B ). Rux dose-dependently inhibited JAK2 tyrosine phosphorylation and dramatically inhibited the phosphorylation of its substrate STAT3 ( Figure 4A ). Western blot analysis with an anti-pTyr (P-Y-1000) revealed that Rux inhibited the total protein tyrosine phosphorylation ( Figure 4C ). The 293T cells incubated in the presence of the indicated concentrations of Rux for 12 h did not exhibit any significant cytotoxicity or retarded cell growth ( Figure 4D ).

If Gin A restricts IAV replication in part by inhibiting S6K1 activity, PF-4708671, an S6K1-specific inhibitor, should also inhibit IAV replication. As previously reported [26] , PF-4708671 inhibited S6 phosphorylation but induced AKT and S6K1 phosphorylation ( Figure 4E ). PF-4708671 did not affect the levels of viral NP or M1 protein levels. This suggests that the inhibition of S6K1 activity does not contribute to the antiviral activity of Gin A.

Prior studies have shown that JAK knockdown downregulates IAV replication [14, 15] . Gin A and Rux, two JAK inhibitors, were able to inhibit JAK activities [19, 30] . We tested if JAK2 overexpression led to increased IAV replication. As shown in Figure 5A , H5N1 virus dose-dependently decreased STAT3 phosphorylation, an observation consistent with the finding in a previous study [25] . JAK2 overexpression in 293T cells dramatically increased JAK2 and STAT3 phosphorylation ( Figure 5A ). JAK2 and STAT3 phosphorylation was slightly decreased in IAV-infected cells in a dose-dependent manner ( Figure 5A ). JAK2 overexpression significantly increased the levels of viral NP and M1 proteins ( Figure 5A ). JAK2 overexpression increased the virus loads in the conditioned media of the JAK2-transfected cells infected with 0.01, 0.1, and 1 MOI by 209%, 114%, 95%, respectively ( Figure 5B ). Of note, the actual infectious titers used to normalize to 100% in the pcDNA3.1-transfected cells infected with 0.01, 0.1, and 1 MOI were 103.11, 105, 105.5, respectively ( Figure 5B ). We then determined if JAK2 overexpression compromised the ability of Gin A to inhibit virus replication. As shown in Figure 5C , Gin A dose-dependently inhibited STAT3 and JAK2 phosphorylation and lowered the levels of viral NP and M1 proteins in pcDNA3.1-transfected 293T cells. However, Gin A inhibited STAT3 and JAK2 Viruses 2020, 12, 1141 9 of 18 phosphorylation and decreased the levels of viral NP and M1 proteins in JAK2-overexpressed 293T cells in a significantly lower magnitude than in pcDNA-transfected 293T cells ( Figure 5C ). The virus titers in untreated or Gin A-treated 293T cells overexpressing JAK2 were significantly higher than their corresponding counterparts in pcDNA3.1-transfected 293T cells ( Figure 5D ).

infected 293T cells at 0.5 or 8 hpi ( Figure 3A,B) . Consistently, Gin A also inhibited JAK2 and STAT3 tyrosine phosphorylation in 293T cells infected with H1N1 ( Figure 3C ) or H9N2 ( Figure 3D ) virus at 8 hpi. Western blot analysis with an antibody against phospho-tyrosine revealed that Gin A inhibited the total protein tyrosine phosphorylation ( Figure 3E ). Consistent with two previous studies showing that S6K1 inhibitors induce the feedback activation of the PI-3 kinase pathway [26, 27] , Gin A decreased S6 phosphorylation but increased AKT phosphorylation in a dose-dependent manner ( Figure 3F ). Of note, Gin A should also increase S6K1 phosphorylation but instead inhibited S6K1 phosphorylation, an observation that was also made in our recent study, suggesting its ability to inhibit mTOR activity as well [27] . Cell lysates were prepared and analyzed for JAK2 and STAT3 tyrosine phosphorylation and the levels of M1 expression by Western blot (B-D), for total protein tyrosine phosphorylation (E), and for the levels of protein phosphorylation in the PI-3 kinase pathway (F). Actin was detected as loading controls. The density of the phosphorylated JAK2 and STAT3 bands was analyzed by using NIH Image-J software and normalized by the arbitrary units of their corresponding total proteins. * p < 0.05, ** p < 0.01, compared to the untreated control.

Rux dose-dependently inhibited JAK2 tyrosine phosphorylation and dramatically inhibited the phosphorylation of its substrate STAT3 ( Figure 4A ). Western blot analysis with an anti-pTyr (P-Y-1000) revealed that Rux inhibited the total protein tyrosine phosphorylation ( Figure 4C ). The 293T cells incubated in the presence of the indicated concentrations of Rux for 12 h did not exhibit any significant cytotoxicity or retarded cell growth ( Figure 4D ). 

Finally, we investigated the antiviral effect of Gin A in mice infected with the H5N1 virus. Gin A treatment significantly decreased the levels of PB2, NP, and M1 proteins, and lowered the virus titers in the lungs on day 3 ( Figure 6A ,C) and 5 ( Figure 6B,D) , compared to the untreated controls. As shown in Figure 6E , the body weights of the Gin A-treated mice were significantly heavier starting on day 3 than that of the untreated mice. Forty percent of H5N1 virus-infected C57BL/6 mice intranasally treated with Gin A survived ( Figure 6F ). In contrast, the untreated C57BL/6 mice all died within 12 days post-infection. The median survival time of Gin A-treated mice was significantly longer than that of the untreated mice (p < 0.01) ( Figure 6F ). We also examined the antiviral activity of Gin A on H1N1 (PR8) virus-infected mice. Gin A treatment significantly alleviated the loss of body weight, compared to the body weight in the control group ( Figure S3A ). Twenty percent of the C57BL/6 mice treated with Gin A by gavage survived H1N1 infection ( Figure S3B ). The median survival time of Gin A-treated mice was significantly longer than that of the untreated mice (p < 0.01).

Viruses 2020, 12, x FOR PEER REVIEW 11 of 18 C57BL/6 mice treated with Gin A by gavage survived H1N1 infection ( Figure S3B ). The median survival time of Gin A-treated mice was significantly longer than that of the untreated mice (p < 0.01). The density of the phosphorylated JAK2 and STAT3 or viral protein bands was analyzed by using NIH Image-J software and normalized by the arbitrary units of their corresponding total proteins or β-actin. * p < 0.05, ** p < 0.01, compared to the untreated control. 

Natural products, particularly those derived from edible vegetables, tend to be more tolerable to the human body and to have the low levels of side effects [31, 32] . Our present study shows that Gin A, a compound enriched in ginger roots (which contains >447 g Gin A per 100 g) [19] , was able to inhibit the replication of three different IAV subtypes in four different cell lines. Mechanistic investigation revealed that Gin A exerted its antiviral activity by inhibiting JAK2 tyrosine kinase activity. In vivo experiments revealed that Gin A inhibited H5N1 virus replication in the lungs of H5N1 virus-infected mice, alleviated their weight loss, and prolonged their survival. Our study suggests that Gin A could be potentially developed as a novel antiviral agent for the treatment of IAV infections. 

Natural products, particularly those derived from edible vegetables, tend to be more tolerable to the human body and to have the low levels of side effects [31, 32] . Our present study shows that Gin A, a compound enriched in ginger roots (which contains >447 µg Gin A per 100 g) [19] , was able to inhibit the replication of three different IAV subtypes in four different cell lines. Mechanistic investigation revealed that Gin A exerted its antiviral activity by inhibiting JAK2 tyrosine kinase activity. In vivo experiments revealed that Gin A inhibited H5N1 virus replication in the lungs of H5N1 virus-infected mice, alleviated their weight loss, and prolonged their survival. Our study suggests that Gin A could be potentially developed as a novel antiviral agent for the treatment of IAV infections.

Protein tyrosine kinases have been increasingly recognized as potential molecular targets for antiviral therapy. There has been great interest in repurposing clinically approved PTK inhibitors as potential antiviral drugs for treating lethal virus infection. For example, c-Abl1 tyrosine kinase is involved in the productive replication of the Ebola virus. The inhibitor of c-Abl1 such as nilotinib suppresses the replication of the Ebola virus [33] . JAK inhibitors have been sought as potential antiviral drugs for treating HIV infection in vitro and in animal models [34] [35] [36] . JAK1 and JAK2 have been recently identified as the leading cellular factors that could be potentially targeted for anti-IAV therapy. Using an RNA-based screening assay, Watanabe et al. [14] reported that JAK1 plays a crucial role in the IAV virion assembly and that JAK1 siRNA and Rux inhibit IAV growth in A549 cells [14] . Using a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR)/Cas screening method, Han et al. [15] reported that JAK2 is involved in IAV replication. Whether JAK inhibitors control IAV infection in vivo remains unknown. Our study shows that Rux dose-dependently decreased viral M1 and NP protein levels ( Figure 4A ). The concentrations of Rux used in our study are comparable to that by Watanabe et al. [14] showing that the drug at the concentrations of 30-100 µM significantly inhibits IAV replication. Gin A is a dual inhibitor of JAK2 and S6K1 kinases [19, 27, 37] . This inhibits the activity of JAK2 with the IC 50 value of approximately 10 µM [19] . Our present study confirmed the ability of Gin A to inhibit JAK2 autophosphorylation and the phosphorylation of its substrate STAT3. Gin A lowered the levels of viral NP and M1 proteins in the lysates of IAV-infected cells and the virus titers in the conditioned media of IAV-infected 293T cells. These observations collectively suggest that Gin A inhibits IAV replication by inhibiting JAK activity.

How JAK1 and JAK2 promote IAV replication is incompletely understood. JAK1 binds multiple viral proteins and is required for vRNP incorporation into virions [14] . Wang et al. [29] reported that JAK2 phosphorylates M1 at Y132 by JAK2 and stimulates its binding with importin-α1 and promotes M1 nuclear translocation. AG490 and dasatinib, a specific inhibitor of JAK and Src kinase, respectively, inhibit M1 nuclear translocation and virus replication. Han et al. [15] reported that JAK2 silencing leads to the increased expression of antiviral genes such as IFN-β, MxA, IFIT1, Rig-1, and ISG15, suggesting that the inhibition of JAK2 may enhance the antiviral defense activity of host cells. Our present study showed that Gin A inhibited JAK2 activity in IAV-infected cells. We speculated that the antiviral activity of Gin A is likely mediated by interfering with virion assembly and by enhancing the antiviral immune response. It should be noted that, while the JAK-STAT pathway is implicated in playing a critical role in antiviral innate immunity, the inhibition of this pathway does not necessarily lead to increased virus replication. For example, Zhang et al. [38] recently reported that the inhibition of STAT1 phosphorylation by fludarabine does not enhance but rather suppresses the H5N1 virus replication. This decreases the production of proinflammatory cytokines such as IL-6, TNF-α, IP10, IFN-α, and IFN-β in A549 cells and in the lungs of H5N1 virus-infected mice. Consistently, Nicol et al. [39] reported that IFN-γ receptor deficiency leads to decreased IAV replication and to lower inflammatory cytokine production in IFNGR -/mice than in wild-type mice.

STAT3 is a transcription factor that plays a central role in regulating inflammation [40] . STAT3 is phosphorylated by JAKs at tyrosine 705 by many cytokine and growth factor receptors such as IL-6, IL-10, and EGF receptors [40, 41] . Recent studies have shown that STAT3 activation suppresses apoptosis by inducing Bcl-2 and Bcl-xL expression [42] . STAT3 promotes cancer stem cell self-renewal and metastasis and plays an important role in carcinogenesis [42] . Numerous studies suggest that STAT3 activation can promote virus replication [41] . For example, STAT3 activation by varicella-zoster (VZV) virus in human fibroblasts in vitro and in xenografts in vivo can enhance VZV replication and pathogenesis [43] . STAT3 is also activated by the hepatitis C virus (HCV) and promotes HCV replication [44] . IB-32, an inhibitor of STAT3, suppresses HCV replication in hepatocytes [45] . Whether STAT3 also regulates IAV replication remains controversial. Mahony et al. [46] reported that the suppression of STAT3 expression leads to increased IAV replication and the decreased expression of antiviral genes, including PKR, OAS2, MxB, and ISG15. This suggests that STAT3 activation can suppress IAV replication. In contrast, Liu et al. [47] reported that neither the overexpression of constitutively active STAT3 nor S31-201, a STAT3-specific inhibitor, significantly altered the H1N1 virus (A/WSN/33) replication in A549 cells. Moreover, Hui et al. [25] reported that the H1N1 virus (A/Hong Kong/54/98) more effectively inhibits STAT3 phosphorylation than the H5N1 virus (A/Vietnam/1203/04) in human alveolar epithelial cells. Consistent with this observation, we found that the H5N1 virus dose-dependently decreased STAT3 phosphorylation in 293T cells at 8 hpi ( Figure 5A ), albeit the virus infection transiently increased STAT3 phosphorylation immediately following virus inoculation, which was blocked by Gin A ( Figure 3A) . However, the overexpression of a constitutively active STAT3 did not affect H5N1 virus replication (Xu, X, unpublished observations). We speculate that Gin A may exert its antiviral activity independent of its inhibitory effect on STAT3 activation.

Ginger is a medicinal plant used in ancient China and an ingredient in Ge-Gen-Tang, a traditional Chinese medicine prescribed for treating human respiratory tract infections [17] . The water extract of fresh ginger and Ge-Gen-Tang inhibit human respiratory syncytial virus replication in vitro [18, 48, 49] . Imanishi et al. [50] reported that ginger extract can activate macrophages to produce cytokines that repress IAV virus replication. Ginger essential oil blocks herpes simplex virus type 2 (HSV-2) replication in RC-37 cells [51, 52] and has a viricidal effect on caprine alphaherpesvirus 1 [53] . Which chemical component in ginger extract accounts for its antiviral activities remains unknown. Ginger extract contains two main categories of bioactive ingredients, e.g., gingerols and shogaols. We and others recently reported that Gin A can sensitize insulin receptor signaling and improve glucose metabolism by activating AMPK and by suppressing S6K1 activity [27, 37] . In addition to its anti-hyperglycemic effect, our present study showed that Gin A was able to inhibit IAV replication in vitro and in vivo. Although the PI-3 kinase pathway has been implicated in playing an important role in IAV replication [12, 13] , our present study using an S6K1-specific inhibitor shows that the inhibition of S6K1 activity did not lead to the suppression of IAV replication, suggesting that Gin A inhibits IAV replication independent of its inhibitory effect on S6K1 activity.

In our study, we also determined the S.I. value to assess the specificity of Gin A on virus replication. We found that the S.I. values were modestly above 10 in 293T but below 10 in A549 and MDCK cells. We postulated that the S.I. values could be affected by multiple factors. For example, the dose of IAV used to infect cells will likely affect the IC 50 value of a candidate drug. Many studies have used a low MOI of IAV (0.01 or 0.001), which will lead to a much higher S.I. value. In our study, we used a 10-fold higher MOI (0.1) of IAV to infect four cell lines. In addition, the length of time used to measure the CC 50 values also affects the S.I. values. The CC 50 values in many studies are calculated based on the cytotoxicity data in which cells are incubated with antiviral agents for 24 h. In addition, Byun et al. reported that Gin A selectively induced apoptosis in cancer cell lines but not in normal cells [19] . This suggests that Gin A, even with modest S.I. values, preferentially causes cytotoxicity in tumor cell lines such as A549 and MDCK cells but becomes less cytotoxic in non-cancerous cell lines such as 293T and DF-1 cells. Gin A may not damage normal respiratory epithelial cells in vivo. In support of this notion, the antiviral activity of Gin A was achieved in our mouse model without any noticeable side effects when the mice were intranasally treated with Gin A at the dose of 5 mg/day. Moreover, Gin A has also been administered to mice in an obese mouse model [37] and a tumor xenograft model [19] for long-term uses without causing significant side-effects. Finally, Gin A is a natural product extracted from ginger roots, which should give a relatively safe profile. Of note, whilst ginger roots have been used in ancient Chinese as herbal medicine for treating febrile diseases, the concentrations of Gin A in raw ginger roots remain unknown. It is also not clear if the antiviral effects of ginger are mediated by other constituents.

In the past decade, JAK inhibitors have been developed as novel anti-inflammatory drugs for treating rheumatoid arthritis and other inflammatory diseases [54] . Several ongoing clinical trials are testing their anti-inflammatory effects in COVID-19 patients [55, 56] . We recently reported that A77 1726, the active metabolite of the anti-rheumatoid arthritis drug leflunomide, is able to inhibit the replication of influenza A virus and porcine epidemic diarrhea virus by targeting JAK1 and JAK2 [57, 58] . Interestingly, a recent small-scale clinical trial revealed that leflunomide is very effective in treating COVID-19 [59] . Our findings that Gin A functions as a JAK inhibitor to inhibit IAV replication suggest that Gin A may control influenza virus infections not only through its antiviral activity but also by damping inflammation. Although Gin A could be potentially developed as an adjunct antiviral agent for treating IAV infections, Gin A will likely not be as effective as conventional anti-IAV drugs, which target neuraminidase, M2, or the viral RNA polymerase. The second cautious note is that Gin A and other JAK inhibitors, which target protein tyrosine kinases of host cells, are likely to have more severe side-effects than conventional antiviral drugs. Moreover, other clinically approved JAK-specific inhibitors may substitute Gin A once their antiviral effects are confirmed in animal models.

In summary, our present study provides evidence that Gin A inhibits the replication of three IAV subtypes in vitro and controls IAV virus infection in vivo. Our mechanistic study suggests that Gin A suppresses IAV replication by inhibiting JAK2 activity. Gin A and other JAK inhibitors could be potentially developed as novel antiviral agents for the control of IAV infection in humans. Our study has validated the antiviral activity of a specific compound from ginger, a medicinal plant with antimicrobial activity.

Supplementary Materials: The following are available online at http://www.mdpi.com/1999-4915/12/10/1141/s1, Figure S1 : Gin A inhibits H1N1 viral protein synthesis, Figure S2 : Gin A decreases the expression of viral proteins, Figure S3 : Gin A inhibits H1N1 virus replication in vivo.

Author Contributions: Conceptualization, X.X. and J.W.; methodology, J.W.; validation, X.X., J.W.; formal analysis, J.W. and X.X.; investigation J.W.; resources, X.L.; data curation, J.W.; writing-X.X.; writing-review and editing, R.A.P.; visualization, J.W.; supervision, X.X.; project administration, X.X.; funding acquisition, X.X. All authors have read and agreed to the published version of the manuscript.

Funding: This research project was supported by a grant from the Priority Academic Program Development of Jiangsu Higher Education Institutions to Xiulong Xu.

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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license

We thank Liqian Zhu, Yangzhou University, for kindly providing the A/PR8/34 H1N1 virus, Jinhua Liu, China Agricultural University, for kindly providing the H1N1 (A/California/04/09) virus, and Eric Chang, Baylor College of Medicine, for kindly providing the pBABE-JAK2 plasmid.

The authors declare no conflict of interest.