key: cord-0722612-1mqf6a8z authors: Klarica, Marijan; Radoš, Milan; Vukić, Miroslav; Orešković, Darko title: The physiology and pathophysiology of cerebrospinal fluid: new evidence date: 2021-08-03 journal: Croat Med J DOI: 10.3325/cmj.2021.62.307 sha: 7cd06387cd43ad524c145a32a76145cbf46ee7de doc_id: 722612 cord_uid: 1mqf6a8z nan This issue of the Croatian Medical Journal is dedicated to advancements in the diagnostics and treatment of neurosurgical diseases. The issue features some of the articles that were originally envisaged as lectures to be delivered at a joint meeting of Croatian and Japanese Neurosurgical Societies on the topic of cerebrospinal fluid (CSF) physiology and pathophysiology. The meeting entitled Physiology and Pathophysiology of Cerebrospinal Fluid -New Evidence was scheduled to be held in Vodice, Croatia, 2020, but was postponed due to the COVID-19 pandemic. Two parts of the meeting were planned: Cerebrospinal Fluid Volume Regulation and Movement -Revision of Classical Concept and Pathophysiology of Hydrocephalus -New Insights. The first part was to be dedicated to the role of the choroid plexus in physiology and pathophysiology. The classical concept defines the choroid plexus as the main CSF source (1, 2) , while a more recent theory, although not negating the importance of the choroid plexus in CSF formation, attributes to this organ a much lesser role than previously believed. The new theory postulates that what is important for CSF formation is the influx and exchange of fluid at the capillary level in the central nervous system (1, (3) (4) (5) (6) (7) . Furthermore, the first part of the meeting aimed to discuss the research on the fate of molecules applied in different parts of the CSF system with or without a blockade of the transport systems in various animal models, from genetically modified mice fetuses (8, 9) to large experimental an-imals (rabbits, cats, dog, pigs) (4-6,10). Mice fetus experiments showed that the movement and fate of molecules in the CSF system were determined by their molecular weight (low-weight molecules move faster), that CSF moved faster in the ventricles than in the subarachnoid space, and that CSF did not circulate (8, 9) . These observations accord with the new concept of CSF physiology, first published about ten years ago. The new concept draws on research involving big experimental animals showing that CSF was not formed exclusively in the brain ventricles, that it did not unidirectionally move from the ventricles to the subarachnoid space, and that it was not dominantly reabsorbed in the arachnoid granulations of the dural sinuses (4-6). Since substances and metabolites applied in different parts of the CSF system were observed to distribute in all directions, many questions remain regarding drug application in the CNS (2). In addition, advanced radiological techniques provide detailed images of the CNS, with excellent contrast between CSF and the surrounding structures (bones and parenchyma). Therefore, these techniques enable us to precisely segment all CSF spaces and quantify their volumes both in the intracranial and spinal part. Aside from the time spatial inversion pulse (Time-SLIP) method, volumetric MR imaging (for example T2 space and phasecontrast sequences, etc) depicts and even quantifies CSF movement inside the CSF system, especially in regions where this movement is pronounced (the foramen of Monro, mesencephalic aqueduct or cranio-cervical junction) (10, 11) . Time-SLIP method has shown CSF pulsation but not bulk flow from production site to absorption site (11) . The findings of these radiological diagnostic procedures often do not accord with the classical CSF hypothesis. Numerous clinical cases have shown that the choroid plexuses are not necessary for CSF production and that a blocked mesencephalic aqueduct will not always induce hypertensive hydrocephalus, as proposed by the classical concept. Furthermore, contrast applied in the spinal CSF space will almost always be distributed in the direction opposite to the one proposed by the classical concept (10) . Finally, a radiological study of arachnoid granulations in patients from birth to 80 years of age strongly suggests that the number, size, and distribution of arachnoid granulations in the superior sagittal sinus and the surrounding cranial bones change significantly over a lifetime (12) . Numerous individuals with a completely normal CSF system (without problems in intracranial fluid homeostasis) do not have arachnoid granulations in the dural sinuses. Contrary to what is generally accepted, arachnoid granulations seem not to play an essential role in CSF absorption (12) . Thus, the CSF absorption into the venous sinuses and/or lymphatics under physiological conditions, due to their small surface area, should be of minor importance compared with the huge absorptive surface area of the microvessel network of the central nervous tissue (4, 5) . All the mentioned studies warranted a revision of the classical concept and a development of a new approach to the investigation of CSF physiology. The second meeting part was planned to deal with different forms of hydrocephalus (acute and subchronic models of aqueductal or cervical blockade or stenosis; knockout models, models of congenital hydrocephalus, kaolin hydrocephalus, idiopathic normal pressure hydrocephalus, arrested hydrocephalus, communicating and noncommunicating hydrocephalus, LAMO, etc). Research in animals (6), newborns (13) , and adults (13) showed that acute aqueduct obstruction, with no artifacts of CSF motion through the aqueduct, did not result in hydrocephalus development in a longer time period (6, 13) . These cases raise the issue of acute hydrocephalus pathophysiology and the accuracy of the classical concept of CSF physiology (14) . Transitory acute hydrocephalus in subarachnoid hemorrhage was planned to be discussed as well (11, 15) . Besides, this part of the symposium would deal with the role of ependymal ciliary motion in the development of congenital hydrocephalus, ie, why this role was earlier perceived to be so important (16) . A further important issue is that pressure gradients within the CSF sys-tem arising during body posture changes and stenosis placement on different positions do not lead to ventricular enlargement as would be expected according to the classical concept (14, 17) . Several lectures were prepared on the pathophysiology of normal pressure hydrocephalus (18) (19) (20) (21) (22) . Idiopathic normal pressure hydrocephalus (iNPH) is a clinical condition with great variations and without a standard symptom pattern that would clearly separate it from other neurodegenerative diseases. Since CSF drainage treatment yields inconsistent results, the introduction of various CSF biomarkers could improve the clinical approach to this condition (22) . The symposium was planned to raise the unresolved questions related to the diagnostics and treatment of hydrocephalus, such as: The recent understanding of the correlation between CSF physiology and the development of some forms of hydrocephalus should be thoroughly presented, analyzed, evaluated, and discussed. This could bring about new insights into hydrocephalus etiopathology and new treatment approaches that are in accordance with the experimental and clinical data. Choroid plexus and CSF: an updated review Harnessing cerebrospinal fluid circulation for drug delivery to brain tissue Integrated understanding of hydrocephalus -a practical approach for a complex disease The formation of cerebrospinal fluid: nearly a hundred years of interpretations and misinterpretations Recent insights into a new hydrodynamics of the cerebrospinal fluid Development of hydrocephalus and classical hypothesis of cerebrospinal fluid hydrodynamics: facts and illusions Role ofchoroidplexusincerebrospinal fluid hydrodynamics Extracranial outflow of particles solved in cerebrospinal fluid: Fluorescein injection study. Congenital Animalies Cerebrospinal fluid may flow out from the brain through the frontal skull base and choroid plexus: a gold colloid and cadaverine injection study in mouse fetus The movement of cerebrospinal fluid and its relationship with substances behavior in cerebrospinal and interstitial fluid Cerebrospinal fluid dynamics No arachnoidgranulations-no problems: number, size and distribution of arachnoid granulations from birth to 80 years of age The role of mesencephalic aqueduct obstruction in the development of hydrocephalus -case report New concepts of cerebrospinal fluid physiologyand development of hydrocephalus Transient acute hydrocephalus after aneurysmal subarachnoid hemorrhage and aneurysm embolization: a single-center experience Ependymal ciliary motion and their role in congenital hydrocephalus. Child's Nervous System. 2021. Ahead of Print Contribution to understanding of ocular and cerebrospinal fluid dynamics in astronauts during longlasting spaceflight Comparison of CSF distribution between idiopathic normal pressure hydrocephalus and Alzheimer disease Fluid distribution pattern in Adult-onset congenital, idiopathic, and secondary normal-pressure hydrocephalus: implications for clinical use. Front Neurol Cerebrospinal fluid dynamics in idiopathic normal pressure hydrocephalus on four-dimensional flow imaging Changes in apparent diffusion coefficient (ADC) during cardiac cycle of the brain in idiopathic normal pressure hydrocephalus before and after cerebrospinal fluid drainage Differentiating comorbidities and predicting prognosis in idiopathic normal pressure hydrocephalus using cerebrospinal fluid biomarkers