key: cord-0733801-iwearl9q authors: Chonmaitree, Tasnee; Revai, Krystal; Grady, James J.; Clos, Audra; Patel, Janak A.; Nair, Sangeeta; Fan, Jiang; Henrickson, Kelly J. title: Viral upper respiratory tract infection and otitis media complication in young children date: 2008-03-15 journal: Clinical Infectious Diseases DOI: 10.1086/528685 sha: dcf6cf371a350038df834f05c6d7486c802ccb60 doc_id: 733801 cord_uid: iwearl9q BACKGROUND: The common cold or upper respiratory infection (URI) is highly prevalent in young children and often results in otitis media (OM). Incidence and characteristics of OM complicating URI by specific viruses have not been well studied. METHODS: We performed a prospective, longitudinal, cohort study of 294 healthy children (6 mos. to 3 yrs. of age). Each child was followed for 1 year for the occurrences of URI and acute otitis media (AOM) and otitis media with effusion (OME) complicating URI by specific viruses. RESULTS: There were 1295 URI episodes (5.06 episodes/child-year) and 440 AOM episodes (1.72 episodes/child-year) documented. Virus studies were performed in 864 URI episodes; 63% were virus positive. Rhinovirus and adenovirus were most commonly detected during URI. The overall incidence of OM complicating URI was 61%, including 37% AOM and 24% OME. Young age was the most important predictor for AOM complicating URI. AOM occurred in about half of children with URI associated with adenovirus, respiratory syncytial virus (RSV), and coronavirus, and about one-third of those with influenza, parainfluenza, enterovirus and rhinovirus. CONCLUSIONS: More than 60% of symptomatic URI episodes in young children were complicated by AOM and/or OME. Young age and specific virus types were predictors of AOM complicating URI. Preventive strategy for OM should be through preventing viral URI in young children. The strategy may be more effective if the priority is given to development of ways to prevent URI associated with adenovirus and RSV. obtain epidemiologic information on URI and to determine the specific virus types associated with URI and their ability to induce AOM and OME. This was a prospective, longitudinal cohort study designed to capture all symptomatic episodes of URI that occurred in children during a 1-year period, to study the incidence and characteristics of URI that is complicated by OM. The study was performed at the University of Texas Medical Branch (Galveston, TX) and was approved by the Institutional Review Board; written informed consent was obtained for all subjects. Healthy children living in Galveston who had received medical care at the University of Texas Medical Branch were recruited from the primary care clinic and via advertisements in the local newspaper and at local day care centers. Children were enrolled at the ages of 6 months to 3 years. They could be asymptomatic or have had URI or AOM at the time of enrollment. Children with chronic medical problems or an anatomical or physiological defect of the ear or nasopharynx were excluded from the study. During the year, parents were asked to notify the study office as soon as the child began to have symptoms of a cold or URI (e.g., nasal congestion, rhinorrhea, cough, sore throat, or fever). Children were seen by a study physician as soon as possible after the onset and were observed a few days later to assess whether there were complications of OM; parents were compensated for time and travel. Study personnel also provided 2 home visits during weeks 2 and 3 of the URI to perform tympanometry. If the tympanogram findings remained abnormal after 3 weeks, testing was repeated every 2 weeks until the findings were normal or the next URI episode occurred. Parents were advised to bring the child for examination whenever they suspected the child to have any AOM symptom. At each visit, information was collected on specific URIrelated symptoms; tympanometry was performed, and the child's ears were examined using pneumatic otoscopy by trained investigators (T.C., K.R., and J.A.P.). OM was considered to have complicated URI if it occurred within 28 days after the onset of URI, unless new-onset URI occurred during this period; in that case, OM was considered to have complicated the most recent URI. AOM was defined as the acute onset of symptoms (fever, irritability, or earache), signs of tympanic membrane inflammation, and presence of fluid, as documented by pneumatic otoscopy and/or tympanometry. Children who received a diagnosis of AOM were treated on the basis of the standard of care [19] . OME was considered to have complicated URI if new fluid and/or an air-fluid bubble was visualized or if a new type B tympanogram result was obtained without signs of tympanic membrane inflammation. New middle ear effusion was defined as the presence of middle ear effusion without a type B tympanogram finding having been documented in the previous 30 days. Children with AOM and OME in each ear were counted as having AOM. The day of onset of OM during the course of URI was determined from day 1 (the first day of symptoms) to the first day of diagnosis. In addition to parent's self-reported URI, the study personnel called the parents twice monthly to determine whether there were any current URI symptoms and occurrence of any URI or AOM episodes missed since prior contact. An extensive review of medical records was performed at the time of completion of each child's study participation. The University of Texas Medical Branch is the sole provider of pediatric health care in Galveston; diseases diagnosed and treated in our children are likely to be noted in our medical records. URI and AOM episodes not seen by the study group but captured from parent's interviews or from medical records were recorded as "missed episodes." Virologic studies. Respiratory specimens were collected for virus studies at the initial URI visit and at subsequent visits only if AOM was diagnosed. Nasal swab specimens were collected for viral culture; nasopharyngeal secretions were collected for respiratory syncytial virus (RSV) antigen detection by EIA (performed only during RSV season) and for virus detection by molecular techniques (performed at the Medical College of Wisconsin; Milwaukee) on culture-and RSV-EIA-negative specimens [20, 21] . Real-time RT-PCR was performed with the PRISM 7300 Sequence Detection System (Applied Biosystems). Positive and negative results were determined with the autoanalysis software and were rechecked manually. For RNA viruses, the first stage conditions were 50ЊC for 30 min followed by 95ЊC for 15 min, 94ЊC for 15 s, and 60ЊC for 60 s for 45 cycles; for adenovirus, the conditions were 95ЊC for 15 min, 94ЊC for 15 s, 55ЊC for 30 s, and 72ЊC for 35 s for 45 cycles. For adenovirus detection (hexon gene), the limit of detection was TCID 50 /mL. The limit of detection for coronavirus 2 1 ϫ 10 OC43/229E (N gene) and NL63 (N gene) were 10 Ϫ2 TCID 50 / mL and 10 Ϫ1 TCID 50 /mL, respectively; for rhinovirus (5 NTR gene), it was 10 Ϫ2 TCID 50 /mL; and for enterovirus (5 NTR), it was 10 Ϫ2 TCID 50 /mL. Analytical specificity was determined for each assay using American Type Culture Collection strains of adenovirus, enterovirus, OC43, 229E, rhinovirus, and influenza A virus; no cross-reactivity was detected. Validation using clinical samples demonstrated sensitivity of 95% (19 of 20 samples) versus tissue culture and 100% specificity (95% CI, 88%-100%) for adenovirus, sensitivity of 93% (13 of 14) and 97% specificity (95% CI, 83%-100%) for rhinovirus, and sensitivity of 75% (6 of 8) and 100% specificity (95% CI, 88%-100%) for enterovirus. RT-PCR with electronic microarray detection (NanoChip 400 system; Namogen) was performed for detection of RSV, parainfluenza types 1-3, and influenza A and B virus, as described elsewhere [21] . Statistical methods. The relationship of AOM or OME with a virus was analyzed using the general estimating equations approach, which treats the child as the unit of analysis while accounting for the multiple episodes of correlated data from each child. The repeated binary outcome of OM status was analyzed with a binomial distribution, logit link function, and AR(1) correlation structure. Analyses were conducted in the Genmod procedure in SAS software (SAS Institute) [22] . Rate ratios were calculated using Episheet 2001, Spreadsheets for the Analysis of Epidemiologic Data, by Rothman [23] . The episode-level culture-and molecular-positive rates of virus detection results were analyzed using Pearson x 2 statistics. During the period January 2003 through March 2006, a total of 294 children were enrolled in the study; 46% entered the study in the first year of life, 42% in the second year, and 12% in the third year. Demographic characteristics and risk factor data are shown in table 1. Overall, the total duration of follow-up was 256 child-years; the median duration of follow-up per subject was 12 months (mean duration, 9.8 months). Overall URI and OM episodes. A total of 1295 URI epi- ). P ! .005 b Rate ratio for subjects aged 6-11 months versus those aged 24-35 months, 2 (95% CI, 1.1-3.6; ). P p .01 sodes were documented during 256 child-years (a rate of 5.06 episodes per child-year); the median age at the time of URI onset was 17.7 months. Another 26 AOM episodes without URI symptoms were documented. Patients accounting for 867 episodes (67%) of URI and for 6 episodes of AOM without URI were seen by the study group. Overall, there were 1839 study visits, 647 home or day care visits, and 4972 tympanograms obtained. Of all URI episodes, 414 (32%) were complicated by AOM. The rate of AOM-complicated URI among episodes observed by the study group was 37%; the rate among missed URI episodes was 22%. With consideration of the 26 episodes of AOM that did not involve symptoms of URI, the total number of AOM episodes captured was 440 (1.72 episodes per child-year). The median age at the onset of AOM was 15.9 months. Of 294 subjects, 201 subjects were observed for the entire 12 month-period; the remainder dropped out before 1 year had passed. The frequency of URI for each subject ranged from 0 to 19 cases per year (figure 1). Six subjects had no URIs (as determined by parent reports and chart reviews), and 7 experienced 112 URI episodes in 1 year. Frequencies of URI and AOM by age at enrollment and sex are shown in table 2. Virologic findings. Of 867 URI episodes seen by the study group, specimens were collected during the first visit for 864; Table 3 lists the 708 viruses detected in 547 (63%) of 864 URI episodes. One virus was detected in 422 episodes (77%), 2 viruses were detected in 92 episodes, 3 viruses were detected in 30 episodes, and 4 viruses were detected in 3 episodes. For the 864 URI episodes observed by the study group that had available virus data, the incidence of URI complicated by AOM was 37% (319 of 864 episodes); 39% of AOM episodes were bilateral, 24% were right-side AOM, and 36% were left-side AOM. AOM was diagnosed on days 1-24 in the course of URI, with the peak occurring on days 3-5; the median time was day 4 (figure 2). In 28 of 864 URI episodes, middle ear effusion (type B tympanogram) was already present within the 30 days before URI onset; these chronic middle ear effusions were excluded from consideration for new-onset OME. The incidence of OMEcomplicated URI was 24% (203 of 836 episodes); 23% of cases were right-side OME, 27% were left-side OME, and 51% were bilateral OME. The overall incidence of URI complicated by OM (i.e., AOM and OME) was 61%. The time of OME diagnosis in the course of URI is also shown in figure 2 ; median time was day 3. Figure 3 illustrates the rate of OM-complicated URI for the 7 respiratory viruses; table 4 shows the rate of OM-complicated URI, the median day of diagnosis, and the age of the subjects at the time of onset of URI, AOM, and OME, by virus type. For each virus (except herpes simplex virus), the median age at onset of AOM was lower than that at onset of URI, suggesting that children who developed AOM were younger children. Coronavirus, RSV, and adenovirus were among the viruses associated with a higher rate of AOM. The episode data shown in table 4 were analyzed using the general estimating equations approach, which treated the child as the unit of analysis, thus accounting for the multiple correlated episodes in each child. The overall model indicated that age ( ) was the stron-P ! .001 gest predictor of AOM development after URI, followed by virus type ( ), controlling for sex ( ), race P p .05 P p .19 ( ), and ethnicity ( ). For age, the OR was 0.96 P p .92 P p .75 (95% CI, 0.94-0.98), meaning that, for each additional month of age in the URI episode, the chances of developing AOM decreased by 4%. Table 5 compares the differences among the rates of AOM associated with specific viruses. For OME outcome, general estimating equations data revealed statistical significance only for age (OR, 0.98; 95% CI, 0.96-0.99; ). P p .03 AOM rate determined by virus diagnostic methods. Because molecular assays are more sensitive than conventional viral diagnostic assays, which likely detect virus only when it is present in larger quantities (i.e., a high viral load), we compared the rate of AOM associated with specific viruses detected by different methods (table 6) . For each virus, the AOM rate was higher in cases diagnosed with viral culture, compared with those diagnosed with molecular assays. Overall, cases diagnosed with culture were associated with a higher rate of AOM, compared with cases diagnosed with molecular methods (P p ). .001 We have demonstrated a high susceptibility of young children to URI and a strikingly high rate of OM complications. Our statistical model identified the age of the child as the most important factor of AOM-complicated URI. The 2 viruses most commonly detected during URI were rhinovirus and adenovirus. Although adenovirus was associated with high rate of AOM-complicated URI, rhinovirus was associated with lower rate than that of coronavirus, RSV, and adenovirus. Our data emphasize the close relationship between viral URI and OM and suggest that one strategy to reduce OM incidence is to prevent viral URI in young children. The relative role of specific virus types in AOM reported in previous studies has been inconclusive, because different virus detection methods were used. Henderson et al. [24] used viral culture and reported that RSV, adenovirus, and influenza virus were closely associated with AOM; the incidence of AOM associated with rhinovirus URI was the lowest. Ruuskanen et al. [25] used conventional viral assays and reported similar results. Vesa et al. [26] found that URI associated with RSV and rhinovirus had a higher rate of AOM than did URI associated with adenovirus, but these authors used PCR alone for detection of rhinovirus and antigen detection for detection of other viruses. Pitkaranta et al. [27] used RT-PCR and reported that rhinovirus (35%) was the most common virus found in cases of nasopharyngeal secretions and/or middle ear effusion in children with AOM (the rates for RSV and coronavirus were 28% and 17%, respectively), although the children in that study were relatively old (median age, 30 months). We used more-com-prehensive diagnostic methods than other researchers and more often detected adenovirus during URI; the virus was also associated with the highest rate of AOM-complicated URI. We were surprised that RSV-associated URI was not diagnosed more frequently; this could be because there was an unusually low prevalence of RSV in our community during the study period or because young children with RSV infection tended to have lower respiratory tract disease rather than URI. Nevertheless, the rate of RSV-associated URI complicated by AOM was among one of the highest; this is consistent with findings from previous reports [24, 28, 29] . Our data together suggest that prevention of adenovirus-and RSV-associated URI-if and when possible-has the potential to make a significant impact on the incidence of AOM. Because URI is self-limiting, viral diagnosis is not clinically indicated. Research studies of viral etiology of URI that use a variety of viral diagnostic methods, including molecular techniques, have provided a virus yield of 42%-73% [26, 30, 31] . Virus yield depends on many factors, including sensitivity of the technique, specificity of the primers, and the number of viruses targeted. New viruses have recently been discovered as causes of URI and/or OM: human metapneumovirus, bocavirus, and coronavirus NL-63 [32] [33] [34] . The limited amount of samples and the high cost prohibited us from testing for all Recently, investigators found viruses in respiratory specimens obtained from children with no symptoms [31] ; rhinovirus has also been found to have a prolonged presence in the respiratory tract [35, 36] . Because viruses are intracellular pathogens, these cases constitute asymptomatic infections. We studied only symptomatic URI and assumed a cause-and-effect relationship. We only compared the rate of OM in URI episodes associated with single virus; of these, 11% revealed the same virus as in previous episodes (table 4 ). In addition, 125 (23%) of our 547 virus-positive samples contained у2 viruses. Other researchers have reported rates of dual-or multiple-virus infection of 5%-20% [26, 30, 31, 37] ; our relatively high rate could be associated with the more-comprehensive assays that we used or with frequent collection of samples from children with recurrent URI. In any event, it was possible that the molecular assays may have also detected some of the virus associated with previous URI episodes. The role of prolonged presence of viruses in the respiratory tract and that of dual-or multiple-virus infections in OM requires further investigations. Positive viral culture results are associated with detection of live virus and are a strong indication of the cause-and-effect relationship (i.e., a viral cause of current symptoms). The findings of high rates of AOM associated with isolation of adenovirus and RSV help confirm the significance of these viruses in AOM-complicated URI. We have also found that, for every virus that both culture and molecular assays detected, the rate of AOM was higher for cases detected by culture. Tissue culture is less sensitive than molecular assays and probably detects virus only in larger quantities (i.e., a high viral load). Therefore, our finding also suggests the role of high viral load in increasing URI severity. Correlations between virus concentrations and elevated levels of cytokines/inflammatory mediators (e.g., IL-6, TNF-a, IFN-g, IL-1, IL-8, and macrophage inflammatory protein-1a), and disease severity have been shown previously in respiratory virus infections [38] [39] [40] [41] [42] [43] [44] . It is likely that, in our cases, higher viral loads generated higher degrees of inflammation, which may have worsened the eustachian tube function, leading to OM complication. In conclusion, we found a high prevalence of symptomatic viral URI among young children, and 160% of cases were complicated by AOM and/or OME. 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Lizette Rangel, Kyralessa B. Ramirez, Syed Ahmad, Michelle Tran, Liliana Najera, Rafael Serna, and Carolina Pillion, for assistance with study subjects; and Andrea Kraft and Ruoyan Chen, for assistance in the laboratories.Financial support. National Institutes of Health (R01 DC005841 and DC 005841-02S1). The study was conducted at the General Clinical Research Center at the University of Texas Medical Branch, which is funded by National Center for Research Resources (National Institutes of Health, US Public Health Service; M01 RR 00073).Potential conflicts of interest. K.J.H. and J.F. hold stock in Prodesse. All other authors: no conflicts.