key: cord-0752183-58ym7hlb
authors: Wang, Lan; Yang, Ling; Bai, Lang; Huang, Zhixin; Peng, Yong
title: Association between D-dimer level and chest CT severity score in patients with SARS-COV-2 pneumonia
date: 2021-06-02
journal: Sci Rep
DOI: 10.1038/s41598-021-91150-1
sha: bb15f05386a853ac3c0bebec9a2073ce9da0df24
doc_id: 752183
cord_uid: 58ym7hlb

The elevated level of D-dimer and its relationship with poor outcomes in SARS-COV-2 pneumonia patients have been demonstrated. In addition to a hypercoagulable state, D-dimer is also a biomarker of inflammation. We investigated the relationship between D-dimer level and chest computed tomography (CT) severity score, which could reflect the severity of inflammation in SARS-COV-2 pneumonia patients. We retrospectively enrolled 86 consecutive SARS-COV-2 pneumonia patients. CT severity scores were computed to quantify the overall lung involvement. The D-dimer level among CT score tertiles and the association of the D-dimer level with CT score were analyzed. Our results showed that the median D-dimer level was 0.70 mg/L (IQR 0.35–1.76). 42 patients (48.8%) had D-dimer levels above the median level. The D-dimer levels were significantly different across CT score tertiles (0.37 mg/l [IQR 0.31–0.87], 0.66 mg/l [IQR 0.39–1.43], 1.83 mg/l [IQR 0.85–4.41], P < 0.001). The natural logarithm of the D-dimer level was significantly associated with the CT score (r(s) = 0.586, P < 0.001). In conclusion, the D-dimer level may be associated with the severity of inflammation of SARS-COV-2 pneumonia prior to coagulopathy/thrombosis. This could be an additional explanation for the mechanism of the relationship between elevated D-dimer level and higher mortality.

], P < 0.047). There were more patients had oxygenation index < 300 in in the group with D-dimer level > 0.7 mg/L than ≤ 0.7 mg/L (57.6% vs. 25%, P = 0.043) ( Table 1) . Table 1 . Comparison of clinical characteristics between patients with D-dimer ≤ 0.7 mg/L and > 0.7 mg/L. Data are expressed as median (interquartile range) or counts and percentage, as appropriate. IHD Ischemic heart disease, COPD Chronic obstructive pulmonary disease, SBP Systolic blood pressure, DBP Diastolic blood pressure, ALT Alanine aminotransferase, AST Aspartate aminotransferase, CRP C-reactive protein, CK-MB Creative kinase MB, hs-cTnI high-sensitivity cardiac troponin I, URL upper reference limit, NT-proBNP N-terminal pro-B-type natriuretic peptide. # There were 28 patients in the group of D-dimer ≤ 0.70 mg/L and 33 patients in D-dimer > 0.70 mg/L had the data of Saturation. *There were 19 patients in the group of D-dimer ≤ 0.70 mg/L and 31 patients in D-dimer > 0.70 mg/L had the data of oxygenation. (Fig. 1A) . The natural logarithm of the D-dimer level was significantly associated with the CT score (r S = 0.586, P < 0.001) (Fig. 1B) .

Comparison of clinical events between patients with D-dimer ≤ 0.7 mg/L and > 0.7 mg/L. Patients with D-dimer level > 0.7 mg/L had a higher rate of mechanical ventilation (14.3% vs. 0.0%, P = 0.011) and higher incidence of composite endpoint (14.3% vs. 0.0%, P = 0.011) than the ones with D-dimer level ≤ 0.7 mg/L. Four patients had pulmonary thromboembolism (PE) in this study, whose D-dimer levels were all above 0.7 mg/L (Table 3 ). There was one patient died during the study period who had segmental PE.

In our study, the median D-dimer level of total patients was 0.7 mg/L. This is close to the level previously reported by Zhou et al. (0.8 mg/L) 2 in Wuhan but lower than the one by Cummings et al. in New York (1.6 ug/ml) 4 , whose study population were all critical cases. There were 48.8% of patients in our study had D-dimer levels > 0.7 mg/L. Their chest CT severity scores were higher than those with D-dimer level ≤ 0.7 mg/L, and more patients (83.3%) had reticulation and/or traction bronchiectasis in the Chest CT imaging. Furthermore, we also found that the D-dimer level was well correlated with the chest CT score. Since a recent study has proved the chest CT score could be an imaging tool for assessing the severity of SARS-COV-2 pneumonia 7,8 , our findings would lead us to conclude that D-dimer level could be a biomarker for severe SARS-COV-2 pneumonia by paralleling with the inflammation involvement extent in lungs which was reflected by chest CT score. The more frequent presence of reticulation and/or traction bronchiectasis in these patients was also evidence of more extensive and severe inflammation response and lung injury. Considering the limited sample size of our study, we didn't analyze the relationship between the D-dimer level and CT score adjusted for age. Further researches with a larger size are needed to investigate their relationship adjusted for age. The relationship between D-dimer level and coagulopathy/thrombosis in COVID-19 patients has been widely confirmed. However, whether the time points of D-dimer elevation and coagulopathy/thrombosis present were consistent has not been fully addressed. Based on our findings in which the D-dimer level's time point was matched to the time of CT scan, we have reasons to speculate that the D-dimer level may be associated with the severity of inflammation prior to coagulopathy/thrombosis. Uncontrolled inflammation response itself could Table 2 . Comparison of radiographic findings between patients with D-dimer ≤ 0.7 mg/L and > 0.7 mg/L. Data are expressed as counts and percentage. The Pearson Chi-square or Fisher's Exact Test was used to test the difference between D-dimer groups, as appropriate. HRCT high resolution computed tomography. www.nature.com/scientificreports/ result in severe lung injury and sequentially aggravate coagulopathy/thrombosis and then lead to poor outcomes, even death. This could be an additional explanation for the mechanism of the relationship between elevated D-dimer and higher mortality which previous studies have demonstrated. Further researches are needed to investigate the relationship between the dynamics of D-dimer level and the severity of inflammation and coagulopathy.

For this retrospective, single-center study, we enrolled consecutive patients from February 9 to March 4, 2020, in a COVID-19 ward of Renmin Hospital of Wuhan University (East Branch) in Wuhan, which is a governmentassigned center for COVID-19 treatment. The diagnosis was confirmed by microbiological and radiographic findings following the World Health Organization (WHO) interim guidance 9 and the Fifth Revised Trial Version of the Novel Coronavirus Pneumonia Diagnosis and Treatment Guidance in China 10 . The observed time of this study ended on April 7. Only the patients who had D-dimer results during the study period were included. The study was approved by the Clinical Rearech Ethics Committee of West China Hospital (2020-226), and the requirement for written informed consent was waived by the Ethics Committee. Demographic data, vital signs, laboratory test reports on admission, and outcomes were obtained from the patients' electronic medical records and scrutinized by investigators. Attending physicians in respiratory medicine reviewed the first high-resolution chest CT images of the patients for analyzing the radiographic patterns such as ground-glass opacities (GGOs), consolidation, reticulation, traction bronchiectasis, and pleural effusion, and for computing CT severity score to quantify overall lung involvement. Each lung was divided into three lung zones. Each lung zone (total of six lung zones) was assigned a score that was based on the following: score 0, 0% involvement; 1, 0-25%; 2, 25-50%; 3, 50-75%; and 4, > 75%. Summation of scores provided overall lung involvement (maximal CT score for both lungs was 24). D-dimer levels at the time point most nearby the date of CT were obtained in patients with high-resolution chest CT. D-dimer testing was performed using the Siemens Innovance D-Dimer Assay. According to the manufacturer's instructions, a value of 0.55 mg/L fibrinogen equivalent units (FEU) was selected as the cut-off value of the normal range with a sensitivity of ≥ 98%.

Data were expressed as the median and interquartile range (IQR [25th to 75th percentiles]) for continuous variables due to the skewed distribution (tested by the Shapiro-Wilk normality test) and as count and percentage for categorical variables. T-test or Mann-Whitney U test was used to analyze the differences between two groups dichotomized by the 50th percentile of D-dimer level for continuous variables. χ 2 test was used for categorical variables. The difference of D-dimer levels among CT score tertiles was tested using the Kruskal-Wallis test. Spearman's correlation coefficient was used to measure the association of the natural logarithm of the D-dimer level with CT score. All the statistical analyses were performed with the use of IBM SPSS Statistics software (version 26.0, https:// www. ibm. com/ suppo rt/ pages/ downl oading-ibm-spss-stati stics-26).

All the methods above were carried out in accordance with relevant guidelines and regulations. 

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L.W. and Y.P. contributed to the study design, data acquisition, data analysis, and interpretation; L.W. and L.Y. contributed to the manuscript; L.Y. contributed to data analysis and interpretation. L.B. and Z.X.H. contributed to data acquisition.

This research did not receive any specific grant from funding agencies in the public, commercial, or not-forprofit sectors.

The authors declare no competing interests.

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