key: cord-0753287-9qnq5f60 authors: Schnaubelt, S.; Oppenauer, J.; Tihanyi, D.; Mueller, M.; Maldonado‐Gonzalez, E.; Zejnilovic, S.; Haslacher, H.; Perkmann, T.; Strassl, R.; Anders, S.; Stefenelli, T.; Zehetmayer, S.; Koppensteiner, R.; Domanovits, H.; Schlager, O. title: Arterial stiffness in acute COVID‐19 and potential associations with clinical outcome date: 2021-03-21 journal: J Intern Med DOI: 10.1111/joim.13275 sha: aefc01922c6d3e27aa0e5e0499679aa8445eb9a3 doc_id: 753287 cord_uid: 9qnq5f60 BACKGROUND: Coronavirus disease 2019 (COVID‐19) interferes with the vascular endothelium. It is not known whether COVID‐19 additionally affects arterial stiffness. METHODS: This case–control study compared brachial‐ankle pulse wave (baPWV) and carotid‐femoral pulse wave velocities (cfPWV) of acutely ill patients with and without COVID‐19. RESULTS: Twenty‐two COVID‐19 patients (50% females, 77 [67–84] years) were compared with 22 age‐ and sex‐matched controls. In COVID‐19 patients, baPWV (19.9 [18.4–21.0] vs. 16.0 [14.2–20.4], P = 0.02) and cfPWV (14.3 [13.4–16.0] vs. 11.0 [9.5–14.6], P = 0.01) were higher than in the controls. In multiple regression analysis, COVID‐19 was independently associated with higher cfPWV (β = 3.164, P = 0.004) and baPWV (β = 3.532, P = 0.003). PWV values were higher in nonsurvivors. In survivors, PWV correlated with length of hospital stay. CONCLUSION: COVID‐19 appears to be related to an enhanced PWV reflecting an increase in arterial stiffness. Higher PWV might be related to an increased length of hospital stay and mortality. The coronavirus disease 2019 (COVID-19) pandemic gravely affects the international community. Whilst the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is primarily spread via aerosols [1] and the infection has its onset in the respiratory tract, it frequently spreads into various other tissues, including the cardiovascular system [2, 3] . Cardiovascular comorbidities in severe COVID-19 are common, thereby impacting on mortality [4] . SARS-CoV-2 potentially damaging the vascular endothelium could be a trigger for vascular disease complications [5] . However, besides endothelial damage, it is not well known to what extent structural changes of the vascular wall occur. In the arterial system, structural wall changes result in deterioration of arterial elasticity, which can be revealed by measuring pulse wave velocity (PWV). Apart from reflecting arterial stiffness, PWV is a well-established surrogate marker of cardiovascular risk [6] . Focussing on arterial stiffness in COVID-19, the goal of the present study was to investigate whether, and to which extent PWV differs between acutely ill COVID-19 patients and acutely ill medical patients without COVID-19. Further, this study aimed to assess the prognostic value of PWV on clinical outcomes in severe COVID-19. Acutely ill, hospitalized adult patients with reversetranscription polymerase chain reaction (RT-PCR)confirmed COVID-19 infection were included in this case-control study. Patients were included at designated COVID-19 isolation wards of two Viennese hospitals (Clinic Penzing and Clinic Donaustadt). At time of inclusion, all patients were hemodynamically stable without catecholamine support or mechanical ventilation. In order to compare COVID-19 with non-COVID-19 patients, we recruited age-and sex-matched controls from a cohort of acutely ill medical patients that had been investigated in the scope of a previous cross-sectional study. Those Arterial stiffness was assessed by measuring PWV, using a certified oscillometric pulse wave device (BOSO ABI Systems 100 PWVâ, Bosch & Sohn GmbH, Jungingen, Germany) [7] . For oscillometric pulse wave recordings, appropriate sphygmomanometer cuffs were placed around both patients' upper arms and lower limbs, just above the ankles. Trained nurses performed the measurements under the supervision of one experienced investigator in quiet rooms with constant room temperature. PWV was measured in a supine patients' position and after an acclimatization period of 10 min. Brachial-ankle pulse wave velocity (baPWV) and carotid-femoral pulse wave velocity (cfPWV) were calculated from the time difference between the peripherally recorded pulse waves, considering the height of the respective patient. Following previous studies, the mean PWVs of the left and right sides were used for baPWV analyses [8, 9] . To determine variability, we performed three individual repetitive measurements in ten volunteers: The mean coefficients of variation were 1.9% for baPWV and 2.8% for cfPWV. COVID-19 patients and non-COVID-19 controls underwent a complete physical examination, measurements of blood pressure and heart rate, an electrocardiogram and routine blood tests. Besides, an ankle-brachial index (ABI) screening for peripheral arterial disease was obtained in all included patients. Data on length of hospital stay and all-cause 30day mortality of COVID-19 patients were collected from the Viennese hospital electronic patients' database and confirmed by reviewing the patients' medical reports. The control cohorts of 22 COVID-19 patients and 22 non-COVID-19 patients were matched for age and sex, and subsequently analysed. Baseline and demographic data were tabulated. Categorical variables are expressed as absolute and relative frequencies. Metric variables are presented as medians with interquartile ranges (IQRs). Boxplots were used to visualize data. Distributional assumptions were checked visually by quantilequantile (Q-Q) plots. As none of the variables met the assumption of normality, only nonparametric tests were conducted. For comparison of categorical data between two groups, the chi-square or Fisher exact tests were used. Differences in numerical data were analysed using the Wilcoxon ranksum test. Multiple linear regression was performed to determine the influence of COVID-19 on the pulse wave velocity; integrated variables into the model were chosen considering the Bonferroniniveau in order to avoid overadjusting. To further reduce bias of overadjusting, additional regression analyses were conducted: one only taking into account blood pressure values and one only considering markers of inflammation. All tests were two-sided, and P-values < 0.05 were considered statistically significant. Statistical analyses were Arterial stiffness and COVID-19 / S. Schnaubelt et al. Between May 2020 and June 2020, 22 acutely ill patients with RT-PCR-confirmed COVID-19 infections (11 females, 76.5 (67.0-84.0) years) were included in this study. Twenty-two age-and sexmatched acutely ill medical non-COVID-19 patients (12 females, 76.5 (67.0-83.0) years) served as controls. These controls were recruited from a cohort of 102 acutely ill medical patients, who had been investigated in the scope of a crosssectional study between December 2017 and January 2020. Demographic and clinical data of COVID-19 patients and matched controls are shown in Table 1 . Respective data of the total cohort of 102 of acutely ill medical patients in comparison with included COVID-19 patients are depicted in Table S1 . BaPWV and cfPWV were higher in patients with COVID-19 than in age-and sex-matched controls without COVID-19 ( Fig. 1a + b) . Similarly, baPWV and cfPWV were higher in COVID-19 patients than in the entire group of 102 acutely ill medical patients without COVID-19 ( Figure S1 ). In a multiple regression model including confounders on PWV (systolic and diastolic blood pressure, C-reactive protein, absolute lymphocyte count, procalcitonin, D-dimer, , the presence of COVID-19 remained associated with baPWV and cfPWV (COVID-19 and baPWV: b = 3.532, P = 0.003; COVID-19 and cfPWV: b = 3.164, P = 0.004). COVID-19 remained independently associated with increased baPWV and cfPWV in additional regression models adjusted for only systolic and diastolic blood pressure (COVID-19 and baPWV: b = 3.414, P = 0.001; COVID-19 and cfPWV: b = 4.034, P < 0.001), and only for markers of inflammation (COVID-19 and baPWV: b = 3.001, P < 0.001; COVID-19 and cfPWV: b = 3.517, P < 0.001; Table S2 ). Of 22 included COVID-19 patients, 11 (50%) died after a mean period of 20.7 AE 4.8 days of hospital stay (8 patients died from circulatory failure following sepsis or multi-organ failure; 3 patients died from respiratory failure defined as fulminant pneumonia in terms of acute respiratory distress syndrome). Eighteen patients were treated solely at a normal ward, and 4 patients were treated at an intermediate care unit before death. BaPWV and cfPWV were higher in nonsurvivors of COVID-19 than in COVID-19 survivors (Fig. 2) . In survivors of COVID-19, the mean length of hospital stay was 12.6 AE 4.3 days. In this subgroup of patients, baPWV and cfPWV were related to hospital length of stay ( Figure S2 ). No association was found between patients' age and hospital length of stay (ϱ = 0.445, P = 0.17). As the main finding, this study revealed substantial differences in arterial stiffness between acutely ill patients with and without COVID-19. Further, PWV appeared to be related to clinical outcomes in COVID-19 patients. We found typical, known clinical and laboratory values of COVID-19 patients, rendering them internationally comparable [10] . The association between COVID-19 and arterial stiffness could be attributed to various pathophysiological mechanisms: First, arterial stiffness potentially increases as a consequence of indirect endothelial damage: The integrity of the vascular endothelium is likely to be harmed by SARS-CoV-2-induced systemic hyperinflammation and cytokine release [3, 11] . In this acute systemic inflammation, the cytokine cascade reduces nitric oxide (NO) bioavailability, increasing arterial stiffness [12] . Secondly, SARS-CoV-2 additionally directly infects endothelial cells by binding to endothelial angiotensin-converting enzyme-2 receptors, which subsequently promotes cellular injury [3] . This results in endothelial dysfunction with abnormal medial vascular smooth muscle cell behaviour and structural changes of the extracellular matrix of the vascular wall (e.g., an increase in matrix metalloproteinase levels, enhancing central aortic stiffness [12] [13] [14] ). Thirdly, apart from promoting a hypercoagulable state commonly seen in COVID-19 patients [15] , endothelial cell damage potentially hampers Journal of Internal Medicine vascular tone regulation [13, 14] . Since the endothelium serves as a critical regulator of vascular homeostasis, endothelial alterations impact the balance of vasoconstriction and vasodilation [16] . Finally, severe systemic inflammation results in vascular adrenoceptor hyporeactivity, low endogenous vasopressin levels and corticosteroid insufficiency, which conjointly decreases the vascular tone and might thereby affect measures of arterial stiffness [17] . For measurements of arterial stiffness, we determined baPWV and cfPWV. PWV is a well-established surrogate marker of arterial stiffness, and several studies have demonstrated that an increase in PWV is associated with an elevated risk of future cardiovascular events and mortality [18] . The advantages of PWV measurements are the high Arterial stiffness and COVID-19 / S. Schnaubelt et al. sensitivity as well as their potential to efficiently capture short-term changes in arterial stiffness [19, 20] . In our personal experience, an additional benefit of oscillometric PWV determination is the practicability of this noninvasive method in acutely ill patients since it is quick and does not additionally burden the patient or medical personnel. Referring to the determination of PWV in patients with COVID-19, the present study further revealed differences in PWV between survivors and nonsurvivors of the disease. Also, PWV appeared to be related to hospital length of stay in COVID-19 patients. These observations suggest that a more pronounced increase in arterial stiffness appears to be associated with unfavourable clinical courses of COVID-19, rendering increased PWV a marker or risk. Therefore, the determination of PWV in COVID-19 admission wards could help identify patients at risk of clinical deterioration. Notably, this study has to be discussed in light of its strengths and limitations: the sample size of this case-control study can be viewed critically. Our primary aim was to compare two homogenous age-and sex-matched cohorts with as few additional influencing factors as possible, leading to a reduction of included subjects. Furthermore, the two cohorts were not assessed in parallel through the same period. One explanation is the difficulty to simultaneously assess patients with and without COVID-19, according to COVID-19 precautions during a national lockdown period. Therefore, according to the rapid spread of the COVID-19 pandemic, we recruited controls from a previously assessed cohort of acutely ill medical patients with cardiorespiratory symptoms. In this respect, it appears plausible that a simultaneous assessment of the two cohorts would not have changed the findings of the present investigation. In addition, follow-up PWV data of COVID-19 patients are yet to be provided. COVID-19 has been identified as a multi-organ disease, affecting the circulatory system throughout the bodyrespective patients should be treated in a generalized approach. PWV serves as a surrogate for arterial stiffness and can reflect the extent of present endothelial and vascular damage. We found that COVID-19 is independently associated with an enhanced PWV, reflecting an increase in arterial stiffness. As PWV appeared to be related to length of hospital stay and mortality, it could be used for identifying patients at risk of clinical deterioration. Up to now, this is the first study assessing arterial stiffness in COVID-19 patients in a small sample and might be the steppingstone for future research. Further studies are warranted to assess whether the observed changes in arterial stiffness sustain last in patients after recovering from COVID-19. Arterial stiffness, measured through pulse wave velocity, is higher in COVID-19 patients than in age-and sex-matched acutely ill medical patients without COVID-19. The difference of arterial stiffness appears to be independent of other coexisting diseases. Higher pulse wave velocity values were observed in COVID-19 patients who did not survive the disease. In COVID-19 survivors, the pulse wave velocity was related to the length of hospital stay. Additional Supporting Information may be found in the online version of this article: Table S1 . Demographic and clinical characteristics of 22 acutely-ill patients with COVID-19 and 102 acutely-ill medical patients without COVID-19. Table S2 . Results of (A) the multivariate analysis to test the association with pulse wave velocities, (B) the multivariate analysis only adjusted for systolic and diastolic blood pressure and COVID-19, (C) the multivariate analysis only adjusted for markers of inflammation and COVID-19, and (D) the various single multivariate analyses of the variables only adjusted for systolic blood pressure. COVID-19 and cardiovascular disease Overcoming barriers: the endothelium as a linchpin of coronavirus disease 2019 pathogenesis? Endothelial cell infection and endotheliitis in COVID-19 COVID-19-associated cardiovascular morbidity in older adults: a position paper from the Italian Society of Cardiovascular Researches Endotheliopathy in COVID-19-associated coagulopathy: evidence from a single-centre, cross-sectional study Expert consensus document on the measurement of aortic stiffness in daily practice using carotid-femoral pulse wave velocity Oscillometric measurement of ankle-brachial index in patients with suspected peripheral disease: comparison with Doppler method Reference values of brachial-ankle pulse wave velocity according to age and blood pressure in a central Asia population Arterial stiffness and b-Amyloid progression in nondemented elderly adults Clinical course and mortality risk of severe COVID-19 COVID-19: consider cytokine storm syndromes and immunosuppression Acute systemic inflammation increases arterial stiffness and decreases wave reflections in healthy individuals Arterial stiffness, atherosclerosis and cardiovascular risk: Pathophysiologic mechanisms and emerging clinical indications Longitudinal association among endothelial function, arterial stiffness and subclinical organ damage in hypertension Pulmonary arterial thrombosis in COVID-19 with fatal outcome: results from a prospective, single-center Role of endothelial dysfunction in atherosclerosis Adrenoceptor hyporeactivity is responsible for Escherichia coli endotoxin-induced acute vascular dysfunction in humans Prediction of cardiovascular events and all-cause mortality with arterial stiffness: a systematic review and meta-analysis ESC/ESH Guidelines for the management of arterial hypertension: The Task Force for the management of arterial hypertension of the European Society of Cardiology and the European Society of Hypertension: The Task Force for the management of arterial hypertension of the European Society of Cardiology and the European Society of Hypertension Endothelial dysfunction: a marker of atherosclerotic risk We thank all personnel involved in acquiring the study data. We especially acknowledge Alexander Antos, Mathias Bader, Carina Cuhaj, Hannes Diesenberger, Na Du, Manuel Istratoaie, Anita Oppenauer, Manuela Repl, Gerhard Ruzicka, Alexander Sommer, Saskia Ulz and Sophia Wirth. Furthermore, we thank Piotr Cicho n, Gerold Wilfing, Professor Sylvia Hartl and Professor Anton Laggner for their support. No funding was provided. None of the authors declare any financial or intellectual conflict of interest.